Restriction map for pF1KSDA1100
##################################
# NO CUT ENZYMES (  121 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AgeI        AhdI        AocI        
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AviII       AvrII       
BamHI       BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BfrI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BseAI       BsiEI       BsiWI       BsmBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrGI       
BssHII      Bst1107I    BstBI       BstYI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DraI        
DrdI        EagI        Eam1105I    EarI        EclXI       
Eco52I      Eco81I      EcoNI       EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HindIII     
HpaI        KpnI        Ksp632I     KspI        MamI        
MboII       MfeI        MflI        MluI        MroI        
MunI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfuI        SgrAI       SnaBI       
SpeI        Sse8387I    SspBI       SspI        SwaI        
Tsp509I     Tth111I     Van91I      XbaI        XhoII       
XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccII        1166  CGCG                 CG^CG
AfaI         1095  GTAC                 GT^AC
AflIII       1246  ACGTGT               A^C[GA][CT]GT
AlwI          769  GATCC                GGATC(4/5)
Aor51HI       152  AGCGCT               AGC^GCT
BbeI          591  GGCGCC               GGCGC^C
BfaI           26  CTAG                 C^TAG
BsaI         1060  GGTCTC               GGTCTC(1/5)
BsaWI        1241  ACCGGA               [AT]^CCGG[AT]
BseRI         202  GAGGAG               GAGGAG(10/8)
BsmI          242  GAATGC               GAATGC(1/-1)
BsrBI        1033  GAGCGG               CCGCTC(-3/-3)
BsrDI         952  GCAATG               GCAATG(2/0)
BsrI          530  CCAGT                ACTGG(1/-1)
BssSI         289  CACGAG               CACGAG(-5/-1)
BstEII        330  GGTCACC              G^GT[ACGT]ACC
BstPI         330  GGTCACC              G^GT[ACGT]ACC
BstUI        1166  CGCG                 CG^CG
DraIII       1209  CACCAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco47III      152  AGCGCT               AGC^GCT
EcoO65I       330  GGTCACC              G^GT[ACGT]ACC
HincII       1219  GTTGAC               GT[CT]^[GA]AC
HindII       1219  GTTGAC               GT[CT]^[GA]AC
KasI          591  GGCGCC               G^GCGCC
MaeI           26  CTAG                 C^TAG
MseI         1103  TTAA                 T^TAA
MvnI         1166  CGCG                 CG^CG
NaeI          226  GCCGGC               GCC^GGC
NarI          591  GGCGCC               GG^CGCC
NcoI            7  CCATGG               C^CATGG
NgoMIV        226  GCCGGC               G^CCGGC
NspI          409  GCATGC               [GA]CATG^[CT]
PaeR7I        115  CTCGAG               C^TCGAG
PleI         1181  GAGTC                GAGTC(4/5)
RsaI         1095  GTAC                 GT^AC
SfiI          112  GGCCTCGAGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI          591  GGCGCC               GGC^GCC
SmlI          115  CTCGAG               C^T[CT][GA]AG
SphI          409  GCATGC               GCATG^C
StuI          509  AGGCCT               AGG^CCT
TfiI           62  GATTC                G^A[AT]TC
Tru9I        1103  TTAA                 T^TAA
XhoI          115  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AcyI          591  GGCGCC               G[GA]^CG[CT]C
             1267  GACGCC              
Alw44I        298  GTGCAC               G^TGCAC
              477  GTGCAC              
ApaI          120  GGGCCC               GGGCC^C
              958  GGGCCC              
ApaLI         298  GTGCAC               G^TGCAC
              477  GTGCAC              
BalI           31  TGGCCA               TGG^CCA
              128  TGGCCA              
BglI          113  GCCTCGAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              594  GCCCCTGGGGC         
BpmI          528  CTCCAG               CTGGAG(16/14)
              643  CTCCAG              
BsaHI         591  GGCGCC               G[GA]^CG[CT]C
             1267  GACGCC              
BsrFI         226  GCCGGC               [GA]^CCGG[CT]
              561  ACCGGC              
BstXI         581  CCACCCACATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              965  CCATCAGCCTGG        
Cfr10I        226  GCCGGC               [GA]^CCGG[CT]
              561  ACCGGC              
DdeI          829  CTGAG                C^T[ACGT]AG
             1292  CTGAG               
DpnII         283  GATC                 ^GATC
              769  GATC                
EcoT14I         7  CCATGG               C^C[AT][AT]GG
              348  CCAAGG              
HgaI         1131  GACGC                GACGC(5/10)
             1267  GACGC               
Hin1I         591  GGCGCC               G[GA]^CG[CT]C
             1267  GACGCC              
HinfI          62  GATTC                G^A[ACGT]TC
             1181  GAGTC               
MboI          283  GATC                 ^GATC
              769  GATC                
MluNI          31  TGGCCA               TGG^CCA
              128  TGGCCA              
MscI           31  TGGCCA               TGG^CCA
              128  TGGCCA              
MslI          333  CACCACAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              582  CACCCACATG          
NdeII         283  GATC                 ^GATC
              769  GATC                
PpuMI          72  GGGACCT              [GA]G^G[AT]CC[CT]
              306  GGGACCT             
PvuII         490  CAGCTG               CAG^CTG
             1171  CAGCTG              
Sau3AI        283  GATC                 ^GATC
              769  GATC                
StyI            7  CCATGG               C^C[AT][AT]GG
              348  CCAAGG              
XcmI          372  CCAGCACATCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              962  CCACCATCAGCCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   67 enzymes) #
#########################################

AciI        AluI        AlwNI       AspHI       AvaI        
AvaII       BanI        BanII       BbvI        BcnI        
BmyI        BsaJI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspMI       BssKI       BstAPI      BstF5I      BstNI       
Cac8I       CfoI        Cfr13I      DraII       DsaI        
EaeI        EcoO109I    EcoRII      FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
HinP1I      HpaII       HphI        ItaI        MaeII       
MaeIII      MnlI        MspA1I      MspI        MvaI        
MwoI        NciI        NlaIII      NlaIV       PstI        
Sau96I      ScrFI       SfaNI       SfcI        SmaI        
TaqI        TseI        Tsp45I      TspRI       TthHB8I     
XmaCI       XmaI        
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