Restriction map for
pF1KSDA1100
(Circular) (Six-base) MAPSORT of: pF1KSDA1100.seq Check: 8206 from: 1 to: 4435
pF1KSDA1100 4435 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 411 411
Size: 4435
AccI GT'mk_AC
Cuts at: 1471 1471
Size: 4435
Acc65I G'GTAC_C
Cuts at: 1449 1449
Size: 4435
AclI AA'CG_TT
Cuts at: 4275 4275
Size: 4435
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1260 2258 2690 3439 3707 1260
Size: 998 432 749 268 1988
Fragments arranged by size:
1988 998 749 432 268
AfeI AGC'GCT
Cuts at: 280 280
Size: 4435
AflIII A'CryG_T
Cuts at: 1372 1785 2891 3868 1372
Size: 413 1106 977 1939
Fragments arranged by size:
1939 1106 977 413
AgeI A'CCGG_T
Cuts at: 2823 2823
Size: 4435
AleI CACnn'nnGTG
Cuts at: 463 463
Size: 4435
AlwNI CAG_nnn'CTG
Cuts at: 618 912 987 1260 1738 3307 618
Size: 294 75 273 478 1569 1746
Fragments arranged by size:
1746 1569 478 294 273 75
ApaI G_GGCC'C
Cuts at: 250 1088 250
Size: 838 3597
ApaLI G'TGCA_C
Cuts at: 424 603 3205 424
Size: 179 2602 1654
Fragments arranged by size:
2602 1654 179
ApoI r'AATT_y
Cuts at: 1437 3788 4044 1437
Size: 2351 256 1828
Fragments arranged by size:
2351 1828 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4435
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4435
AvaI C'yCGr_G
Cuts at: 213 241 269 1181 1453 213
Size: 28 28 912 272 3195
Fragments arranged by size:
3195 912 272 28 28
BamHI G'GATC_C
Cuts at: 1458 1458
Size: 4435
BanI G'GyrC_C
Cuts at: 717 870 1404 1449 1863 2111 2146 717
Size: 153 534 45 414 248 35 3006
Fragments arranged by size:
3006 534 414 248 153 45 35
BanII G_rGCy'C
Cuts at: 141 250 364 397 1088 1447 2477 141
Size: 109 114 33 691 359 1030 2099
Fragments arranged by size:
2099 1030 691 359 114 109 33
BbeI G_GCGC'C
Cuts at: 721 1867 2115 721
Size: 1146 248 3041
Fragments arranged by size:
3041 1146 248
BciVI GTATCCnnnnn_n'
Cuts at: 2331 3094 4435 2331
Size: 763 1341 2331
Fragments arranged by size:
2331 1341 763
BclI T'GATC_A
Cuts at: 1953 1953
Size: 4435
BglI GCCn_nnn'nGGC
Cuts at: 245 726 245
Size: 481 3954
BglII A'GATC_T
Cuts at: 1948 3987 1948
Size: 2039 2396
BlpI GC'TnA_GC
Cuts at: 1545 1545
Size: 4435
Bme1580I G_kGCm'C
Cuts at: 250 428 522 607 629 875 1088 1409 2058
Size: 178 94 85 22 246 213 321 649
Cuts at: 2058 2151 3209 250
Size: 93 1058 1476
Fragments arranged by size:
1476 1058 649 321 246 213 178 94
93 85 22
BmgBI CAC'GTC
Cuts at: 789 1110 1373 789
Size: 321 263 3851
Fragments arranged by size:
3851 321 263
BmrI ACTGGGnnnn_n'
Cuts at: 1645 1726 2061 4207 1645
Size: 81 335 2146 1873
Fragments arranged by size:
2146 1873 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2800 2800
Size: 4435
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 639 754 639
Size: 115 4320
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1609 2488 2982 3280 1609
Size: 879 494 298 2764
Fragments arranged by size:
2764 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 1192 4139 1192
Size: 2947 1488
BsaAI yAC'GTr
Cuts at: 1425 1786 1425
Size: 361 4074
BsaBI GATnn'nnATC
Cuts at: 6 1947 3986 6
Size: 1941 2039 455
Fragments arranged by size:
2039 1941 455
BsaHI Gr'CG_yC
Cuts at: 718 1394 1864 2112 2816 4312 718
Size: 676 470 248 704 1496 841
Fragments arranged by size:
1496 841 704 676 470 248
BsaWI w'CCGG_w
Cuts at: 1367 1622 2143 2823 3097 3244 3655 1367
Size: 255 521 680 274 147 411 2147
Fragments arranged by size:
2147 680 521 411 274 255 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 637 667 796 826 1405 1435 637
Size: 30 129 30 579 30 3637
Fragments arranged by size:
3637 579 129 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 343 343
Size: 4435
BseYI C'CCAG_C
Cuts at: 290 378 386 521 531 701 749 852 1030
Size: 88 8 135 10 170 48 103 178
Cuts at: 1030 1164 1858 2616 3195 3691 290
Size: 134 694 758 579 496 1034
Fragments arranged by size:
1034 758 694 579 496 178 170 135
134 103 88 48 10 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 358 658 862 886 358
Size: 300 204 24 3907
Fragments arranged by size:
3907 300 204 24
BsiEI CG_ry'CG
Cuts at: 104 1975 2021 3231 104
Size: 1871 46 1210 1308
Fragments arranged by size:
1871 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 428 607 961 1447 2225 2415 3209 428
Size: 179 354 486 778 190 794 1654
Fragments arranged by size:
1654 794 778 486 354 190 179
BsmI GAATG_Cn'
Cuts at: 374 374
Size: 4435
Bsp1286I G_dGCh'C
Cuts at: 141 250 364 397 428 522 607 629 875
Size: 109 114 33 31 94 85 22 246
Cuts at: 875 961 1088 1409 1447 2058 2151 2225 2415
Size: 86 127 321 38 611 93 74 190
Cuts at: 2415 2477 3209 141
Size: 62 732 1367
Fragments arranged by size:
1367 732 611 321 246 190 127 114
109 94 93 86 85 74 62 38
33 31 22
BspEI T'CCGG_A
Cuts at: 3655 3655
Size: 4435
BspHI T'CATG_A
Cuts at: 3611 3611
Size: 4435
BspMI ACCTGCnnnn'nnnn_
Cuts at: 411 803 893 1483 1697 1999 2380 411
Size: 392 90 590 214 302 381 2466
Fragments arranged by size:
2466 590 392 381 302 214 90
BsrBI CCG'CTC
Cuts at: 50 1161 2725 2779 4265 4430 50
Size: 1111 1564 54 1486 165 55
Fragments arranged by size:
1564 1486 1111 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1085 2345 1085
Size: 1260 3175
BsrFI r'CCGG_y
Cuts at: 352 687 2431 2612 2823 352
Size: 335 1744 181 211 1964
Fragments arranged by size:
1964 1744 335 211 181
BssSI C'ACGA_G
Cuts at: 415 2704 3064 415
Size: 2289 360 1786
Fragments arranged by size:
2289 1786 360
BstAPI GCAn_nnn'nTGC
Cuts at: 618 885 987 1810 618
Size: 267 102 823 3243
Fragments arranged by size:
3243 823 267 102
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4435
BstEII G'GTnAC_C
Cuts at: 456 456
Size: 4435
BstXI CCAn_nnnn'nTGG
Cuts at: 714 1098 714
Size: 384 4051
BstYI r'GATC_y
Cuts at: 1 1458 1925 1948 2283 2529 3543 3629 3641
Size: 1457 467 23 335 246 1014 86 12
Cuts at: 3641 3987 1
Size: 346 449
Fragments arranged by size:
1457 1014 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 133 173 946 133
Size: 40 773 3622
Fragments arranged by size:
3622 773 40
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2383 2564 2727 2383
Size: 181 163 4091
Fragments arranged by size:
4091 181 163
BtsI GCAGTG_nn'
Cuts at: 152 522 985 1676 152
Size: 370 463 691 2911
Fragments arranged by size:
2911 691 463 370
DraI TTT'AAA
Cuts at: 1432 1432
Size: 4435
DraIII CAC_nnn'GTG
Cuts at: 1340 1340
Size: 4435
DrdI GACnn_nn'nnGTC
Cuts at: 2139 2999 2139
Size: 860 3575
EaeI y'GGCC_r
Cuts at: 157 254 1154 2018 2192 2583 2610 3957 157
Size: 97 900 864 174 391 27 1347 635
Fragments arranged by size:
1347 900 864 635 391 174 97 27
EagI C'GGCC_G
Cuts at: 2018 2018
Size: 4435
EarI CTCTTCn'nnn_
Cuts at: 2456 2666 2456
Size: 210 4225
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 156 2641 2953 3099 4245 156
Size: 2485 312 146 1146 346
Fragments arranged by size:
2485 1146 346 312 146
EcoICRI GAG'CTC
Cuts at: 1445 1445
Size: 4435
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 639 754 1260 2258 2690 3439 3707 639
Size: 115 506 998 432 749 268 1367
Fragments arranged by size:
1367 998 749 506 432 268 115
EcoO109I rG'GnC_Cy
Cuts at: 199 246 247 433 1084 1140 1572 199
Size: 47 1 186 651 56 432 3062
Fragments arranged by size:
3062 651 432 186 56 47 1
EcoRI G'AATT_C
Cuts at: 1437 1437
Size: 4435
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2452 2452
Size: 4435
FspI TGC'GCA
Cuts at: 2214 2214
Size: 4435
HaeII r_GCGC'y
Cuts at: 282 336 721 1867 2115 3139 4118 282
Size: 54 385 1146 248 1024 979 599
Fragments arranged by size:
1146 1024 979 599 385 248 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 796 828 2275 2305 2307 2337 796
Size: 32 1447 30 2 30 2894
Fragments arranged by size:
2894 1447 32 30 30 2
HincII GTy'rAC
Cuts at: 1347 1472 1347
Size: 125 4310
KasI G'GCGC_C
Cuts at: 717 1863 2111 717
Size: 1146 248 3041
Fragments arranged by size:
3041 1146 248
KpnI G_GTAC'C
Cuts at: 1453 1453
Size: 4435
MluI A'CGCG_T
Cuts at: 3868 3868
Size: 4435
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1229 3106 3290 1229
Size: 1877 184 2374
Fragments arranged by size:
2374 1877 184
MscI TGG'CCA
Cuts at: 159 256 2194 159
Size: 97 1938 2400
Fragments arranged by size:
2400 1938 97
MslI CAynn'nnrTG
Cuts at: 463 712 2422 2549 463
Size: 249 1710 127 2349
Fragments arranged by size:
2349 1710 249 127
MspA1I CmG'CkG
Cuts at: 183 618 774 900 1299 1544 1858 2218 3233
Size: 435 156 126 399 245 314 360 1015
Cuts at: 3233 3478 183
Size: 245 1140
Fragments arranged by size:
1140 1015 435 399 360 314 245 245
156 126
NaeI GCC'GGC
Cuts at: 354 2614 354
Size: 2260 2175
NarI GG'CG_CC
Cuts at: 718 1864 2112 718
Size: 1146 248 3041
Fragments arranged by size:
3041 1146 248
NcoI C'CATG_G
Cuts at: 133 133
Size: 4435
NgoMIV G'CCGG_C
Cuts at: 352 2612 352
Size: 2260 2175
NruI TCG'CGA
Cuts at: 2796 2796
Size: 4435
NspI r_CATG'y
Cuts at: 539 1486 1986 2895 539
Size: 947 500 909 2079
Fragments arranged by size:
2079 947 909 500
PasI CC'CwG_GG
Cuts at: 723 816 1409 723
Size: 93 593 3749
Fragments arranged by size:
3749 593 93
PciI A'CATG_T
Cuts at: 2891 2891
Size: 4435
PmeI GTTT'AAAC
Cuts at: 1432 1432
Size: 4435
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3574 3606 3574
Size: 32 4403
PpuMI rG'GwC_Cy
Cuts at: 199 433 199
Size: 234 4201
PspOMI G'GGCC_C
Cuts at: 246 1084 246
Size: 838 3597
PspXI vC'TCGA_Gb
Cuts at: 241 241
Size: 4435
PstI C_TGCA'G
Cuts at: 517 746 980 1480 2165 517
Size: 229 234 500 685 2787
Fragments arranged by size:
2787 685 500 234 229
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4435
PvuII CAG'CTG
Cuts at: 618 1299 1858 2218 618
Size: 681 559 360 2835
Fragments arranged by size:
2835 681 559 360
RsrII CG'GwC_CG
Cuts at: 2628 2628
Size: 4435
SacI G_AGCT'C
Cuts at: 1447 1447
Size: 4435
SalI G'TCGA_C
Cuts at: 1470 1470
Size: 4435
SapI GCTCTTCn'nnn_
Cuts at: 2456 2666 2456
Size: 210 4225
SbfI CC_TGCA'GG
Cuts at: 1480 1480
Size: 4435
ScaI AGT'ACT
Cuts at: 3952 3952
Size: 4435
SfcI C'TryA_G
Cuts at: 33 491 513 742 976 1476 2161 3156 3347
Size: 458 22 229 234 500 685 995 191
Cuts at: 3347 33
Size: 1121
Fragments arranged by size:
1121 995 685 500 458 234 229 191
22
SfiI GGCCn_nnn'nGGCC
Cuts at: 245 245
Size: 4435
SfoI GGC'GCC
Cuts at: 719 1865 2113 719
Size: 1146 248 3041
Fragments arranged by size:
3041 1146 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4435
SmaI CCC'GGG
Cuts at: 215 271 1183 1455 215
Size: 56 912 272 3195
Fragments arranged by size:
3195 912 272 56
SmlI C'TyrA_G
Cuts at: 241 1588 2503 2997 3259 241
Size: 1347 915 494 262 1417
Fragments arranged by size:
1417 1347 915 494 262
SnaBI TAC'GTA
Cuts at: 1425 1425
Size: 4435
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4435
SphI G_CATG'C
Cuts at: 539 1486 1986 539
Size: 947 500 2988
Fragments arranged by size:
2988 947 500
StuI AGG'CCT
Cuts at: 637 637
Size: 4435
StyI C'CwwG_G
Cuts at: 133 474 1567 1921 133
Size: 341 1093 354 2647
Fragments arranged by size:
2647 1093 354 341
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 392 400 724 976 2016 2149 2608 2819 392
Size: 8 324 252 1040 133 459 211 2008
Fragments arranged by size:
2008 1040 459 324 252 211 133 8
TatI w'GTAC_w
Cuts at: 3950 3950
Size: 4435
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 637 669 3952 3984 637
Size: 32 3283 32 1088
Fragments arranged by size:
3283 1088 32 32
Tth111I GACn'n_nGTC
Cuts at: 2230 2230
Size: 4435
XbaI T'CTAG_A
Cuts at: 1464 1464
Size: 4435
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 505 1095 1928 505
Size: 590 833 3012
Fragments arranged by size:
3012 833 590
XhoI C'TCGA_G
Cuts at: 241 241
Size: 4435
XmaI C'CCGG_G
Cuts at: 213 269 1181 1453 213
Size: 56 912 272 3195
Fragments arranged by size:
3195 912 272 56
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NruI NspI
PasI PciI PmeI PpiI PpuMI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AatII AflII AhdI AlfI AloI AscI AvrII BaeI
BbsI BbvCI BcgI BfrBI BmtI Bpu10I BsiWI BsmBI
BsrGI BssHII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NsiI PacI PflMI PfoI PmlI PshAI PsiI PsrI
SacII SanDI SexAI SgrAI SrfI SspI SwaI XmnI
ZraI