Restriction map for pF1KSDA1100
 (Linear) (Six-base) MAPSORT of: KIAA1100.seq  Check: 8506  from: 1  to: 1296



KIAA1100.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:29  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     285    1296 
   Size:        285    1011
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1134    1296 
   Size:       1134     162
 
AfeI AGC'GCT

Cuts at:      0     154    1296 
   Size:        154    1142
 
AflIII A'CryG_T

Cuts at:      0    1246    1296 
   Size:       1246      50
 
AleI CACnn'nnGTG

Cuts at:      0     337    1296 
   Size:        337     959
 
AlwNI CAG_nnn'CTG

Cuts at:      0     492     786     861    1134    1296 
   Size:        492     294      75     273     162

  Fragments arranged by size: 

                 492     294     273     162      75
 
ApaI G_GGCC'C

Cuts at:      0     124     962    1296 
   Size:        124     838     334

  Fragments arranged by size: 

                 838     334     124
 
ApaLI G'TGCA_C

Cuts at:      0     298     477    1296 
   Size:        298     179     819

  Fragments arranged by size: 

                 819     298     179
 
AvaI C'yCGr_G

Cuts at:      0      87     115     143    1055    1296 
   Size:         87      28      28     912     241

  Fragments arranged by size: 

                 912     241      87      28      28
 
BanI G'GyrC_C

Cuts at:      0     591     744    1278    1296 
   Size:        591     153     534      18

  Fragments arranged by size: 

                 591     534     153      18
 
BanII G_rGCy'C

Cuts at:      0      15     124     238     271     962    1296 
   Size:         15     109     114      33     691     334

  Fragments arranged by size: 

                 691     334     114     109      33      15
 
BbeI G_GCGC'C

Cuts at:      0     595    1296 
   Size:        595     701
 
BglI GCCn_nnn'nGGC

Cuts at:      0     119     600    1296 
   Size:        119     481     696

  Fragments arranged by size: 

                 696     481     119
 
Bme1580I G_kGCm'C

Cuts at:      0     124     302     396     481     503     749     962    1283 
   Size:        124     178      94      85      22     246     213     321

Cuts at:   1283    1296 
   Size:         13

  Fragments arranged by size: 

                 321     246     213     178     124      94      85      22
                  13
 
BmgBI CAC'GTC

Cuts at:      0     663     984    1247    1296 
   Size:        663     321     263      49

  Fragments arranged by size: 

                 663     321     263      49
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     513     628    1296 
   Size:        513     115     668

  Fragments arranged by size: 

                 668     513     115
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1066    1296 
   Size:       1066     230
 
BsaHI Gr'CG_yC

Cuts at:      0     592    1268    1296 
   Size:        592     676      28

  Fragments arranged by size: 

                 676     592      28
 
BsaWI w'CCGG_w

Cuts at:      0    1241    1296 
   Size:       1241      55
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     511     541     670     700    1296 
   Size:        511      30     129      30     596

  Fragments arranged by size: 

                 596     511     129      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     217    1296 
   Size:        217    1079
 
BseYI C'CCAG_C

Cuts at:      0     164     252     260     395     405     575     623     726 
   Size:        164      88       8     135      10     170      48     103

Cuts at:    726     904    1038    1296 
   Size:        178     134     258

  Fragments arranged by size: 

                 258     178     170     164     135     134     103      88
                  48      10       8
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     232     532     736     760    1296 
   Size:        232     300     204      24     536

  Fragments arranged by size: 

                 536     300     232     204      24
 
BsiHKAI G_wGCw'C

Cuts at:      0     302     481     835    1296 
   Size:        302     179     354     461

  Fragments arranged by size: 

                 461     354     302     179
 
BsmI GAATG_Cn'

Cuts at:      0     248    1296 
   Size:        248    1048
 
Bsp1286I G_dGCh'C

Cuts at:      0      15     124     238     271     302     396     481     503 
   Size:         15     109     114      33      31      94      85      22

Cuts at:    503     749     835     962    1283    1296 
   Size:        246      86     127     321      13

  Fragments arranged by size: 

                 321     246     127     114     109      94      86      85
                  33      31      22      15      13
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     285     677     767    1296 
   Size:        285     392      90     529

  Fragments arranged by size: 

                 529     392     285      90
 
BsrBI CCG'CTC

Cuts at:      0    1035    1296 
   Size:       1035     261
 
BsrDI GCAATG_nn'

Cuts at:      0     959    1296 
   Size:        959     337
 
BsrFI r'CCGG_y

Cuts at:      0     226     561    1296 
   Size:        226     335     735

  Fragments arranged by size: 

                 735     335     226
 
BssSI C'ACGA_G

Cuts at:      0     289    1296 
   Size:        289    1007
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     492     759     861    1296 
   Size:        492     267     102     435

  Fragments arranged by size: 

                 492     435     267     102
 
BstEII G'GTnAC_C

Cuts at:      0     330    1296 
   Size:        330     966
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     588     972    1296 
   Size:        588     384     324

  Fragments arranged by size: 

                 588     384     324
 
BtgI C'CryG_G

Cuts at:      0       7      47     820    1296 
   Size:          7      40     773     476

  Fragments arranged by size: 

                 773     476      40       7
 
BtsI GCAGTG_nn'

Cuts at:      0      26     396     859    1296 
   Size:         26     370     463     437

  Fragments arranged by size: 

                 463     437     370      26
 
DraIII CAC_nnn'GTG

Cuts at:      0    1214    1296 
   Size:       1214      82
 
EaeI y'GGCC_r

Cuts at:      0      31     128    1028    1296 
   Size:         31      97     900     268

  Fragments arranged by size: 

                 900     268      97      31
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      30    1296 
   Size:         30    1266
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     513     628    1134    1296 
   Size:        513     115     506     162

  Fragments arranged by size: 

                 513     506     162     115
 
EcoO109I rG'GnC_Cy

Cuts at:      0      73     120     121     307     958    1014    1296 
   Size:         73      47       1     186     651      56     282

  Fragments arranged by size: 

                 651     282     186      73      56      47       1
 
HaeII r_GCGC'y

Cuts at:      0     156     210     595    1296 
   Size:        156      54     385     701

  Fragments arranged by size: 

                 701     385     156      54
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     670     702    1296 
   Size:        670      32     594

  Fragments arranged by size: 

                 670     594      32
 
HincII GTy'rAC

Cuts at:      0    1221    1296 
   Size:       1221      75
 
KasI G'GCGC_C

Cuts at:      0     591    1296 
   Size:        591     705
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1103    1296 
   Size:       1103     193
 
MscI TGG'CCA

Cuts at:      0      33     130    1296 
   Size:         33      97    1166

  Fragments arranged by size: 

                1166      97      33
 
MslI CAynn'nnrTG

Cuts at:      0     337     586    1296 
   Size:        337     249     710

  Fragments arranged by size: 

                 710     337     249
 
MspA1I CmG'CkG

Cuts at:      0      57     492     648     774    1173    1296 
   Size:         57     435     156     126     399     123

  Fragments arranged by size: 

                 435     399     156     126     123      57
 
NaeI GCC'GGC

Cuts at:      0     228    1296 
   Size:        228    1068
 
NarI GG'CG_CC

Cuts at:      0     592    1296 
   Size:        592     704
 
NcoI C'CATG_G

Cuts at:      0       7    1296 
   Size:          7    1289
 
NgoMIV G'CCGG_C

Cuts at:      0     226    1296 
   Size:        226    1070
 
NspI r_CATG'y

Cuts at:      0     413    1296 
   Size:        413     883
 
PasI CC'CwG_GG

Cuts at:      0     597     690    1283    1296 
   Size:        597      93     593      13

  Fragments arranged by size: 

                 597     593      93      13
 
PpuMI rG'GwC_Cy

Cuts at:      0      73     307    1296 
   Size:         73     234     989

  Fragments arranged by size: 

                 989     234      73
 
PspOMI G'GGCC_C

Cuts at:      0     120     958    1296 
   Size:        120     838     338

  Fragments arranged by size: 

                 838     338     120
 
PspXI vC'TCGA_Gb

Cuts at:      0     115    1296 
   Size:        115    1181
 
PstI C_TGCA'G

Cuts at:      0     391     620     854    1296 
   Size:        391     229     234     442

  Fragments arranged by size: 

                 442     391     234     229
 
PvuII CAG'CTG

Cuts at:      0     492    1173    1296 
   Size:        492     681     123

  Fragments arranged by size: 

                 681     492     123
 
SfcI C'TryA_G

Cuts at:      0     365     387     616     850    1296 
   Size:        365      22     229     234     446

  Fragments arranged by size: 

                 446     365     234     229      22
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     119    1296 
   Size:        119    1177
 
SfoI GGC'GCC

Cuts at:      0     593    1296 
   Size:        593     703
 
SmaI CCC'GGG

Cuts at:      0      89     145    1057    1296 
   Size:         89      56     912     239

  Fragments arranged by size: 

                 912     239      89      56
 
SmlI C'TyrA_G

Cuts at:      0     115    1296 
   Size:        115    1181
 
SphI G_CATG'C

Cuts at:      0     413    1296 
   Size:        413     883
 
StuI AGG'CCT

Cuts at:      0     511    1296 
   Size:        511     785
 
StyI C'CwwG_G

Cuts at:      0       7     348    1296 
   Size:          7     341     948

  Fragments arranged by size: 

                 948     341       7
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     266     274     598     850    1296 
   Size:        266       8     324     252     446

  Fragments arranged by size: 

                 446     324     266     252       8
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     511     543    1296 
   Size:        511      32     753

  Fragments arranged by size: 

                 753     511      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     379     969    1296 
   Size:        379     590     327

  Fragments arranged by size: 

                 590     379     327
 
XhoI C'TCGA_G

Cuts at:      0     115    1296 
   Size:        115    1181
 
XmaI C'CCGG_G

Cuts at:      0      87     143    1055    1296 
   Size:         87      56     912     241

  Fragments arranged by size: 

                 912     241      87      56
 

 Enzymes that do cut:

     AarI     AcuI     AfeI   AflIII     AleI    AlwNI     ApaI    ApaLI
     AvaI     BanI    BanII     BbeI     BglI Bme1580I    BmgBI     BpmI
     BsaI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
     BsmI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI
   BstEII    BstXI     BtgI     BtsI   DraIII     EaeI     EciI  Eco57MI
 EcoO109I    HaeII    Hin4I   HincII     KasI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PasI    PpuMI
   PspOMI    PspXI     PstI    PvuII     SfcI     SfiI     SfoI     SmaI
     SmlI     SphI     StuI     StyI    TaqII     TstI     XcmI     XhoI
     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII     AgeI     AhdI     AlfI
     AloI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI
     BmrI     BmtI     BplI   Bpu10I    BpuEI    BsaAI    BsaBI    BsiEI
    BsiWI    BsmBI    BspEI    BspHI    BsrGI   BssHII    BstBI    BstYI
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI     DrdI     EagI
     EarI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI  HindIII     HpaI     KpnI     MfeI     MluI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PshAI     PsiI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SgfI
    SgrAI    SnaBI     SpeI     SrfI     SspI     SwaI     TatI  Tth111I
     XbaI     XmnI     ZraI
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