Restriction map for pF1KSDA1098
 (Linear) (Six-base) MAPSORT of: KIAA1098.seq  Check: 9244  from: 1  to: 1479



KIAA1098.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:29  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     144     175     984    1479 
   Size:        144      31     809     495

  Fragments arranged by size: 

                 809     495     144      31
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     894    1479 
   Size:        894     585
 
AflIII A'CryG_T

Cuts at:      0      17    1119    1479 
   Size:         17    1102     360

  Fragments arranged by size: 

                1102     360      17
 
AhdI GACnn_n'nnGTC

Cuts at:      0     189    1479 
   Size:        189    1290
 
AleI CACnn'nnGTG

Cuts at:      0    1454    1479 
   Size:       1454      25
 
ApaLI G'TGCA_C

Cuts at:      0    1403    1479 
   Size:       1403      76
 
AvaI C'yCGr_G

Cuts at:      0      25     337    1134    1179    1479 
   Size:         25     312     797      45     300

  Fragments arranged by size: 

                 797     312     300      45      25
 
BamHI G'GATC_C

Cuts at:      0     557    1479 
   Size:        557     922
 
BanI G'GyrC_C

Cuts at:      0     392    1023    1411    1479 
   Size:        392     631     388      68

  Fragments arranged by size: 

                 631     392     388      68
 
BanII G_rGCy'C

Cuts at:      0     856    1042    1063    1479 
   Size:        856     186      21     416

  Fragments arranged by size: 

                 856     416     186      21
 
BbeI G_GCGC'C

Cuts at:      0    1027    1415    1479 
   Size:       1027     388      64

  Fragments arranged by size: 

                1027     388      64
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1041    1479 
   Size:       1041     438
 
BbvCI CC'TCA_GC

Cuts at:      0    1142    1479 
   Size:       1142     337
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     773     807     813     847    1479 
   Size:        773      34       6      34     632

  Fragments arranged by size: 

                 773     632      34      34       6
 
BglI GCCn_nnn'nGGC

Cuts at:      0     487    1037    1479 
   Size:        487     550     442

  Fragments arranged by size: 

                 550     487     442
 
BlpI GC'TnA_GC

Cuts at:      0     322    1479 
   Size:        322    1157
 
Bme1580I G_kGCm'C

Cuts at:      0     395    1407    1479 
   Size:        395    1012      72

  Fragments arranged by size: 

                1012     395      72
 
BmgBI CAC'GTC

Cuts at:      0      18    1250    1479 
   Size:         18    1232     229

  Fragments arranged by size: 

                1232     229      18
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     166    1479 
   Size:        166    1313
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1167    1479 
   Size:       1167     312
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1122    1315    1406    1479 
   Size:       1122     193      91      73

  Fragments arranged by size: 

                1122     193      91      73
 
Bpu10I CC'TnA_GC

Cuts at:      0    1142    1479 
   Size:       1142     337
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     612    1479 
   Size:        612     867
 
BsaAI yAC'GTr

Cuts at:      0    1120    1479 
   Size:       1120     359
 
BsaHI Gr'CG_yC

Cuts at:      0    1024    1412    1479 
   Size:       1024     388      67

  Fragments arranged by size: 

                1024     388      67
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     340     370     838     868    1282    1312    1479 
   Size:        340      30     468      30     414      30     167

  Fragments arranged by size: 

                 468     414     340     167      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      64     268     616     646     700    1008    1318    1479 
   Size:         64     204     348      30      54     308     310     161

  Fragments arranged by size: 

                 348     310     308     204     161      64      54      30
            
 
BseYI C'CCAG_C

Cuts at:      0     143    1035    1242    1479 
   Size:        143     892     207     237

  Fragments arranged by size: 

                 892     237     207     143
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     172     292     421     462     777    1012    1479 
   Size:        172     120     129      41     315     235     467

  Fragments arranged by size: 

                 467     315     235     172     129     120      41
 
BsiHKAI G_wGCw'C

Cuts at:      0    1407    1479 
   Size:       1407      72
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     682    1479 
   Size:        682     797
 
Bsp1286I G_dGCh'C

Cuts at:      0     395     856    1042    1063    1407    1479 
   Size:        395     461     186      21     344      72

  Fragments arranged by size: 

                 461     395     344     186      72      21
 
BspHI T'CATG_A

Cuts at:      0     758    1479 
   Size:        758     721
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     144     175     212     984    1479 
   Size:        144      31      37     772     495

  Fragments arranged by size: 

                 772     495     144      37      31
 
BsrBI CCG'CTC

Cuts at:      0       6      38     717    1479 
   Size:          6      32     679     762

  Fragments arranged by size: 

                 762     679      32       6
 
BsrFI r'CCGG_y

Cuts at:      0     405    1479 
   Size:        405    1074
 
BsrGI T'GTAC_A

Cuts at:      0    1349    1479 
   Size:       1349     130
 
BssHII G'CGCG_C

Cuts at:      0     266     268    1479 
   Size:        266       2    1211

  Fragments arranged by size: 

                1211     266       2
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     399     462    1479 
   Size:        399      63    1017

  Fragments arranged by size: 

                1017     399      63
 
BstYI r'GATC_y

Cuts at:      0     557    1479 
   Size:        557     922
 
BtgI C'CryG_G

Cuts at:      0     123     312     623     797     978    1479 
   Size:        123     189     311     174     181     501

  Fragments arranged by size: 

                 501     311     189     181     174     123
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     498    1479 
   Size:        498     981
 
BtsI GCAGTG_nn'

Cuts at:      0     416     460    1224    1338    1479 
   Size:        416      44     764     114     141

  Fragments arranged by size: 

                 764     416     141     114      44
 
ClaI AT'CG_AT

Cuts at:      0     833    1479 
   Size:        833     646
 
DraIII CAC_nnn'GTG

Cuts at:      0     399    1230    1479 
   Size:        399     831     249

  Fragments arranged by size: 

                 831     399     249
 
EaeI y'GGCC_r

Cuts at:      0     108     626     656    1265    1479 
   Size:        108     518      30     609     214

  Fragments arranged by size: 

                 609     518     214     108      30
 
EarI CTCTTCn'nnn_

Cuts at:      0     334     793    1479 
   Size:        334     459     686

  Fragments arranged by size: 

                 686     459     334
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     894    1122    1315    1406    1479 
   Size:        894     228     193      91      73

  Fragments arranged by size: 

                 894     228     193      91      73
 
FspI TGC'GCA

Cuts at:      0     208    1435    1479 
   Size:        208    1227      44

  Fragments arranged by size: 

                1227     208      44
 
HaeII r_GCGC'y

Cuts at:      0     501    1027    1142    1338    1415    1479 
   Size:        501     526     115     196      77      64

  Fragments arranged by size: 

                 526     501     196     115      77      64
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     175     207    1162    1194    1479 
   Size:        175      32     955      32     285

  Fragments arranged by size: 

                 955     285     175      32      32
 
HindIII A'AGCT_T

Cuts at:      0     577    1479 
   Size:        577     902
 
KasI G'GCGC_C

Cuts at:      0    1023    1411    1479 
   Size:       1023     388      68

  Fragments arranged by size: 

                1023     388      68
 
MscI TGG'CCA

Cuts at:      0    1267    1479 
   Size:       1267     212
 
MslI CAynn'nnrTG

Cuts at:      0    1454    1479 
   Size:       1454      25
 
MspA1I CmG'CkG

Cuts at:      0     125     140     314     934    1160    1479 
   Size:        125      15     174     620     226     319

  Fragments arranged by size: 

                 620     319     226     174     125      15
 
NarI GG'CG_CC

Cuts at:      0    1024    1412    1479 
   Size:       1024     388      67

  Fragments arranged by size: 

                1024     388      67
 
NcoI C'CATG_G

Cuts at:      0     623     797     978    1479 
   Size:        623     174     181     501

  Fragments arranged by size: 

                 623     501     181     174
 
NruI TCG'CGA

Cuts at:      0     584    1479 
   Size:        584     895
 
NspI r_CATG'y

Cuts at:      0     460     828    1479 
   Size:        460     368     651

  Fragments arranged by size: 

                 651     460     368
 
PspXI vC'TCGA_Gb

Cuts at:      0     337    1479 
   Size:        337    1142
 
PstI C_TGCA'G

Cuts at:      0     725     863    1097    1479 
   Size:        725     138     234     382

  Fragments arranged by size: 

                 725     382     234     138
 
PvuII CAG'CTG

Cuts at:      0     934    1160    1479 
   Size:        934     226     319

  Fragments arranged by size: 

                 934     319     226
 
SacII CC_GC'GG

Cuts at:      0     126     315    1479 
   Size:        126     189    1164

  Fragments arranged by size: 

                1164     189     126
 
SexAI A'CCwGG_T

Cuts at:      0     230    1479 
   Size:        230    1249
 
SfcI C'TryA_G

Cuts at:      0     721     859    1093    1479 
   Size:        721     138     234     386

  Fragments arranged by size: 

                 721     386     234     138
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     487    1479 
   Size:        487     992
 
SfoI GGC'GCC

Cuts at:      0    1025    1413    1479 
   Size:       1025     388      66

  Fragments arranged by size: 

                1025     388      66
 
SmaI CCC'GGG

Cuts at:      0      27    1479 
   Size:         27    1452
 
SmlI C'TyrA_G

Cuts at:      0     337     591    1479 
   Size:        337     254     888

  Fragments arranged by size: 

                 888     337     254
 
SphI G_CATG'C

Cuts at:      0     460     828    1479 
   Size:        460     368     651

  Fragments arranged by size: 

                 651     460     368
 
StuI AGG'CCT

Cuts at:      0     481    1479 
   Size:        481     998
 
StyI C'CwwG_G

Cuts at:      0     476     623     797     978    1083    1479 
   Size:        476     147     174     181     105     396

  Fragments arranged by size: 

                 476     396     181     174     147     105
 
TatI w'GTAC_w

Cuts at:      0    1349    1479 
   Size:       1349     130
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     340     372    1282    1314    1479 
   Size:        340      32     910      32     165

  Fragments arranged by size: 

                 910     340     165      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0      91    1479 
   Size:         91    1388
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1254    1366    1479 
   Size:       1254     112     113

  Fragments arranged by size: 

                1254     113     112
 
XhoI C'TCGA_G

Cuts at:      0     337    1479 
   Size:        337    1142
 
XmaI C'CCGG_G

Cuts at:      0      25    1479 
   Size:         25    1454
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AhdI     AleI    ApaLI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI     BglI     BlpI
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI    BsaAI
    BsaHI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I
    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII   BstAPI    BstYI
     BtgI    BtgZI     BtsI     ClaI   DraIII     EaeI     EarI  Eco57MI
     FspI    HaeII    Hin4I  HindIII     KasI     MscI     MslI   MspA1I
     NarI     NcoI     NruI     NspI    PspXI     PstI    PvuII    SacII
    SexAI     SfcI     SfiI     SfoI     SmaI     SmlI     SphI     StuI
     StyI     TatI     TstI  Tth111I     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AlfI
     AloI    AlwNI     ApaI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BciVI     BclI    BfrBI    BglII     BmtI     BsaI    BsaBI
    BsaWI    BsiEI    BsiWI     BsmI    BspEI    BsrDI    BssSI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I    CspCI     DraI     DrdI     EagI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
    FspAI   HincII     HpaI     KpnI     MfeI     MluI     MmeI     NaeI
     NdeI   NgoMIV     NheI     NotI     NsiI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI     PsrI     PvuI    RsrII     SacI     SalI    SanDI     SapI
     SbfI     ScaI     SgfI    SgrAI    SnaBI     SpeI     SrfI     SspI
     SwaI    TaqII     XbaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com