Restriction map for pF1KSDA0975
##################################
# NO CUT ENZYMES (   70 enzymes) #
##################################

AatII       AclI        AgeI        Alw44I      Aor51HI     
ApaLI       AscI        AseI        AsnI        AviII       
AvrII       BamHI       BclI        BlnI        BsaBI       
BsaI        BsiWI       BsmI        Bsp1407I    BspDI       
BspLU11I    BsrGI       Bst1107I    BstBI       BstEII      
BstPI       BstZ17I     ClaI        CpoI        DraI        
DraIII      DrdI        Eco47III    EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HpaI        MamI        MfeI        MluI        MunI        
NcoI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PinAI       
PmeI        PshBI       Psp1406I    PvuI        RsrII       
SalI        SfiI        SfuI        SgrAI       SnaBI       
SpeI        SspBI       SwaI        XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccIII       1184  TCCGGA               T^CCGGA
AcsI          790  GAATTT               [GA]^AATT[CT]
AflIII       1217  ACGTGT               A^C[GA][CT]GT
AhdI         3691  GACCTGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         3203  CCTCAGG              CC^T[ACGT]AGG
ApoI          790  GAATTT               [GA]^AATT[CT]
Asp700I      1042  GAAGGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        3691  GACCTGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         4156  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbeI         2890  GGCGCC               GGCGC^C
BciVI        2162  GTATCC               GTATCC(6/5)
BglII        4136  AGATCT               A^GATCT
BseAI        1184  TCCGGA               T^CCGGA
BsmBI        3265  GAGACG               CGTCTC(1/5)
BspEI        1184  TCCGGA               T^CCGGA
BspHI        4317  TCATGA               T^CATGA
BstAPI        618  GCAGCTCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I       3203  CCTCAGG              CC^T[ACGT]AGG
EagI         2609  CGGCCG               C^GGCCG
Eam1105I     3691  GACCTGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        2609  CGGCCG               C^GGCCG
Eco52I       2609  CGGCCG               C^GGCCG
Eco81I       3203  CCTCAGG              CC^T[ACGT]AGG
KasI         2890  GGCGCC               G^GCGCC
KspI          377  CCGCGG               CCGC^GG
MroI         1184  TCCGGA               T^CCGGA
NarI         2890  GGCGCC               GG^CGCC
PflFI        4156  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
RcaI         4317  TCATGA               T^CATGA
SacII         377  CCGCGG               CCGC^GG
SbfI         2490  CCTGCAGG             CCTGCA^GG
ScaI          327  AGTACT               AGT^ACT
SexAI        2576  ACCTGGT              A^CC[AT]GGT
SfoI         2890  GGCGCC               GGC^GCC
SmaI         3049  CCCGGG               CCC^GGG
SphI         4070  GCATGC               GCATG^C
Sse8387I     2490  CCTGCAGG             CCTGCA^GG
SspI          642  AATATT               AAT^ATT
Tth111I      4156  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI        3049  CCCGGG               C^CCGGG
XmaI         3049  CCCGGG               C^CCGGG
XmnI         1042  GAAGGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

Acc65I       2930  GGTACC               G^GTACC
             4211  GGTACC              
AflII         559  CTTAAG               C^TTAAG
              568  CTTAAG              
Asp718I      2930  GGTACC               G^GTACC
             4211  GGTACC              
BcgI          432  CGAGGTCTTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             3300  CGAGGCCCCTGC        
BfrI          559  CTTAAG               C^TTAAG
              568  CTTAAG              
BglI           37  GCCGAGCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             3407  GCCTGCGGGGC         
BsaWI        1184  TCCGGA               [AT]^CCGG[AT]
             1259  ACCGGA              
BsiEI           5  CGACCG               CG[GA][CT]^CG
             2609  CGGCCG              
BstXI         323  CCAGAGTACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1265  CCAAGGTGCTGG        
DsaI          377  CCGCGG               C^C[GA][CT]GG
             2787  CCGTGG              
HincII       1138  GTCAAC               GT[CT]^[GA]AC
             3862  GTTGAC              
HindII       1138  GTCAAC               GT[CT]^[GA]AC
             3862  GTTGAC              
HindIII       246  AAGCTT               A^AGCTT
             3682  AAGCTT              
KpnI         2930  GGTACC               GGTAC^C
             4211  GGTACC              
NaeI           42  GCCGGC               GCC^GGC
              427  GCCGGC              
NgoMIV         42  GCCGGC               G^CCGGC
              427  GCCGGC              
NspI         1710  ACATGC               [GA]CATG^[CT]
             4070  GCATGC              
StuI         3236  AGGCCT               AGG^CCT
             4055  AGGCCT              


#########################################
# 3 OR MORE CUT ENZYMES (  128 enzymes) #
#########################################

AccI        AccII       AciI        AcyI        AfaI        
AluI        AlwI        AlwNI       ApaI        AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbrPI       BbsI        BbvCI       BbvI        BcnI        
BfaI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaHI       BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrBI       
BsrDI       BsrFI       BsrI        BssHII      BssKI       
BssSI       BstF5I      BstNI       BstUI       BstYI       
Cac8I       CelII       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       EaeI        EarI        
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PflMI       
PleI        PmaCI       PmlI        PpuMI       PshAI       
PstI        PvuII       RsaI        SacI        SapI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XcmI        XhoII       
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