Restriction map for pF1KSDA0945
 (Linear) (Six-base) MAPSORT of: KIAA0945.seq  Check: 2802  from: 1  to: 2337



KIAA0945.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:29  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     947    1014    1542    2337 
   Size:        947      67     528     795

  Fragments arranged by size: 

                 947     795     528      67
 
Acc65I G'GTAC_C

Cuts at:      0    1770    2337 
   Size:       1770     567
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     392     991    1543    2337 
   Size:        392     599     552     794

  Fragments arranged by size: 

                 794     599     552     392
 
AflII C'TTAA_G

Cuts at:      0    2323    2337 
   Size:       2323      14
 
AflIII A'CryG_T

Cuts at:      0    1716    2337 
   Size:       1716     621
 
AleI CACnn'nnGTG

Cuts at:      0    1333    2337 
   Size:       1333    1004
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     232    1449    2337 
   Size:        232    1217     888

  Fragments arranged by size: 

                1217     888     232
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1023    1222    1352    2153    2337 
   Size:       1023     199     130     801     184

  Fragments arranged by size: 

                1023     801     199     184     130
 
ApoI r'AATT_y

Cuts at:      0    1456    2337 
   Size:       1456     881
 
AvaI C'yCGr_G

Cuts at:      0     124     732    1963    2337 
   Size:        124     608    1231     374

  Fragments arranged by size: 

                1231     608     374     124
 
BamHI G'GATC_C

Cuts at:      0     447    2337 
   Size:        447    1890
 
BanI G'GyrC_C

Cuts at:      0     149     350     424     986    1273    1511    1770    2337 
   Size:        149     201      74     562     287     238     259     567

  Fragments arranged by size: 

                 567     562     287     259     238     201     149      74
            
 
BanII G_rGCy'C

Cuts at:      0      65     133     716     919    1118    1244    1261    1303 
   Size:         65      68     583     203     199     126      17      42

Cuts at:   1303    1547    1580    2051    2337 
   Size:        244      33     471     286

  Fragments arranged by size: 

                 583     471     286     244     203     199     126      68
                  65      42      33      17
 
BbeI G_GCGC'C

Cuts at:      0     153     354     990    2337 
   Size:        153     201     636    1347

  Fragments arranged by size: 

                1347     636     201     153
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      83     326    2337 
   Size:         83     243    2011

  Fragments arranged by size: 

                2011     243      83
 
BbvCI CC'TCA_GC

Cuts at:      0    1049    2337 
   Size:       1049    1288
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1245    1279    2337 
   Size:       1245      34    1058

  Fragments arranged by size: 

                1245    1058      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0      50     422     744     818    2337 
   Size:         50     372     322      74    1519

  Fragments arranged by size: 

                1519     372     322      74      50
 
BglII A'GATC_T

Cuts at:      0    1940    2337 
   Size:       1940     397
 
Bme1580I G_kGCm'C

Cuts at:      0     684    1268    1516    1603    2337 
   Size:        684     584     248      87     734

  Fragments arranged by size: 

                 734     684     584     248      87
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     491    1210    1517    2337 
   Size:        491     719     307     820

  Fragments arranged by size: 

                 820     719     491     307
 
BmtI G_CTAG'C

Cuts at:      0     617    2337 
   Size:        617    1720
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     923    2337 
   Size:        923    1414
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     250     415     536     931    1352    1638    2337 
   Size:        250     165     121     395     421     286     699

  Fragments arranged by size: 

                 699     421     395     286     250     165     121
 
Bpu10I CC'TnA_GC

Cuts at:      0     898    1013    1049    1112    2009    2270    2337 
   Size:        898     115      36      63     897     261      67

  Fragments arranged by size: 

                 898     897     261     115      67      63      36
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     584    2337 
   Size:        584    1753
 
BsaAI yAC'GTr

Cuts at:      0    1717    2337 
   Size:       1717     620
 
BsaHI Gr'CG_yC

Cuts at:      0     150     351     987    2337 
   Size:        150     201     636    1350

  Fragments arranged by size: 

                1350     636     201     150
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     486     545    1012    1234    1249    1293    2125    2337 
   Size:        486      59     467     222      15      44     832     212

  Fragments arranged by size: 

                 832     486     467     222     212      59      44      15
            
 
BseYI C'CCAG_C

Cuts at:      0     298     335     516     594     717    1482    1621    1766 
   Size:        298      37     181      78     123     765     139     145

Cuts at:   1766    2035    2188    2337 
   Size:        269     153     149

  Fragments arranged by size: 

                 765     298     269     181     153     149     145     139
                 123      78      37
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     275    1106    1208    2337 
   Size:        275     831     102    1129

  Fragments arranged by size: 

                1129     831     275     102
 
BsiHKAI G_wGCw'C

Cuts at:      0    1118    1261    2015    2337 
   Size:       1118     143     754     322

  Fragments arranged by size: 

                1118     754     322     143
 
Bsp1286I G_dGCh'C

Cuts at:      0      65     133     684     716     919    1118    1244    1261 
   Size:         65      68     551      32     203     199     126      17

Cuts at:   1261    1268    1303    1516    1547    1580    1603    2015    2051 
   Size:          7      35     213      31      33      23     412      36

Cuts at:   2051    2337 
   Size:        286

  Fragments arranged by size: 

                 551     412     286     213     203     199     126      68
                  65      36      35      33      32      31      23      17
                   7
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     293     395     947    1014    1542    2138    2337 
   Size:        293     102     552      67     528     596     199

  Fragments arranged by size: 

                 596     552     528     293     199     102      67
 
BsrBI CCG'CTC

Cuts at:      0     654    1857    2337 
   Size:        654    1203     480

  Fragments arranged by size: 

                1203     654     480
 
BsrFI r'CCGG_y

Cuts at:      0     241    2337 
   Size:        241    2096
 
BssSI C'ACGA_G

Cuts at:      0     677    1231    2044    2337 
   Size:        677     554     813     293

  Fragments arranged by size: 

                 813     677     554     293
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1023    2337 
   Size:       1023    1314
 
BstEII G'GTnAC_C

Cuts at:      0     136    2337 
   Size:        136    2201
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     417     609     821    2337 
   Size:        417     192     212    1516

  Fragments arranged by size: 

                1516     417     212     192
 
BstYI r'GATC_y

Cuts at:      0     447    1438    1940    2337 
   Size:        447     991     502     397

  Fragments arranged by size: 

                 991     502     447     397
 
Bsu36I CC'TnA_GG

Cuts at:      0    1199    1269    2337 
   Size:       1199      70    1068

  Fragments arranged by size: 

                1199    1068      70
 
BtgI C'CryG_G

Cuts at:      0     844    2159    2310    2337 
   Size:        844    1315     151      27

  Fragments arranged by size: 

                1315     844     151      27
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2057    2337 
   Size:       2057     280
 
BtsI GCAGTG_nn'

Cuts at:      0    1118    1171    1667    2337 
   Size:       1118      53     496     670

  Fragments arranged by size: 

                1118     670     496      53
 
EaeI y'GGCC_r

Cuts at:      0      51     239     847    1149    1476    1934    2162    2337 
   Size:         51     188     608     302     327     458     228     175

  Fragments arranged by size: 

                 608     458     327     302     228     188     175      51
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2296    2337 
   Size:       2296      41
 
EcoICRI GAG'CTC

Cuts at:      0    1116    1259    2337 
   Size:       1116     143    1078

  Fragments arranged by size: 

                1116    1078     143
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     250     392     415     536     931     991    1352    1543 
   Size:        250     142      23     121     395      60     361     191

Cuts at:   1543    1638    2337 
   Size:         95     699

  Fragments arranged by size: 

                 699     395     361     250     191     142     121      95
                  60      23
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     901    2337 
   Size:        901    1436
 
EcoO109I rG'GnC_Cy

Cuts at:      0     522     543     574     729    2337 
   Size:        522      21      31     155    1608

  Fragments arranged by size: 

                1608     522     155      31      21
 
FspI TGC'GCA

Cuts at:      0    1645    2337 
   Size:       1645     692
 
HaeII r_GCGC'y

Cuts at:      0     153     180     354     705     990    1613    1728    2172 
   Size:        153      27     174     351     285     623     115     444

Cuts at:   2172    2337 
   Size:        165

  Fragments arranged by size: 

                 623     444     351     285     174     165     153     115
                  27
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      26     120     152     688     720    2337 
   Size:         26      94      32     536      32    1617

  Fragments arranged by size: 

                1617     536      94      32      32      26
 
KasI G'GCGC_C

Cuts at:      0     149     350     986    2337 
   Size:        149     201     636    1351

  Fragments arranged by size: 

                1351     636     201     149
 
KpnI G_GTAC'C

Cuts at:      0    1774    2337 
   Size:       1774     563
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1433    2337 
   Size:       1433     904
 
MscI TGG'CCA

Cuts at:      0     849    1151    1478    1936    2164    2337 
   Size:        849     302     327     458     228     173

  Fragments arranged by size: 

                 849     458     327     302     228     173
 
MslI CAynn'nnrTG

Cuts at:      0    1333    2297    2337 
   Size:       1333     964      40

  Fragments arranged by size: 

                1333     964      40
 
MspA1I CmG'CkG

Cuts at:      0     628    1053    1062    1625    1658    2337 
   Size:        628     425       9     563      33     679

  Fragments arranged by size: 

                 679     628     563     425      33       9
 
NarI GG'CG_CC

Cuts at:      0     150     351     987    2337 
   Size:        150     201     636    1350

  Fragments arranged by size: 

                1350     636     201     150
 
NcoI C'CATG_G

Cuts at:      0     844    2159    2337 
   Size:        844    1315     178

  Fragments arranged by size: 

                1315     844     178
 
NheI G'CTAG_C

Cuts at:      0     613    2337 
   Size:        613    1724
 
NruI TCG'CGA

Cuts at:      0    2076    2337 
   Size:       2076     261
 
NspI r_CATG'y

Cuts at:      0     123    2043    2337 
   Size:        123    1920     294

  Fragments arranged by size: 

                1920     294     123
 
PasI CC'CwG_GG

Cuts at:      0    1295    1443    2337 
   Size:       1295     148     894

  Fragments arranged by size: 

                1295     894     148
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     779    2337 
   Size:        779    1558
 
PfoI T'CCnGG_A

Cuts at:      0    1466    1880    2337 
   Size:       1466     414     457

  Fragments arranged by size: 

                1466     457     414
 
PmlI CAC'GTG

Cuts at:      0    1717    2337 
   Size:       1717     620
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2263    2295    2337 
   Size:       2263      32      42

  Fragments arranged by size: 

                2263      42      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     522     543     574    2337 
   Size:        522      21      31    1763

  Fragments arranged by size: 

                1763     522      31      21
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    2134    2166    2337 
   Size:       2134      32     171

  Fragments arranged by size: 

                2134     171      32
 
PstI C_TGCA'G

Cuts at:      0      71     810    1166    1438    1539    1663    2149    2223 
   Size:         71     739     356     272     101     124     486      74

Cuts at:   2223    2337 
   Size:        114

  Fragments arranged by size: 

                 739     486     356     272     124     114     101      74
                  71
 
PvuII CAG'CTG

Cuts at:      0     628    1053    1658    2337 
   Size:        628     425     605     679

  Fragments arranged by size: 

                 679     628     605     425
 
SacI G_AGCT'C

Cuts at:      0    1118    1261    2337 
   Size:       1118     143    1076

  Fragments arranged by size: 

                1118    1076     143
 
ScaI AGT'ACT

Cuts at:      0    2143    2337 
   Size:       2143     194
 
SfcI C'TryA_G

Cuts at:      0      67     806    1162    1344    1434    1535    1659    2145 
   Size:         67     739     356     182      90     101     124     486

Cuts at:   2145    2219    2337 
   Size:         74     118

  Fragments arranged by size: 

                 739     486     356     182     124     118     101      90
                  74      67
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      50     744    2337 
   Size:         50     694    1593

  Fragments arranged by size: 

                1593     694      50
 
SfoI GGC'GCC

Cuts at:      0     151     352     988    2337 
   Size:        151     201     636    1349

  Fragments arranged by size: 

                1349     636     201     151
 
SmlI C'TyrA_G

Cuts at:      0    2323    2337 
   Size:       2323      14
 
SphI G_CATG'C

Cuts at:      0     123    2043    2337 
   Size:        123    1920     294

  Fragments arranged by size: 

                1920     294     123
 
StuI AGG'CCT

Cuts at:      0     205    1695    2183    2337 
   Size:        205    1490     488     154

  Fragments arranged by size: 

                1490     488     205     154
 
StyI C'CwwG_G

Cuts at:      0     200     525     725     749     773     844    2159    2337 
   Size:        200     325     200      24      24      71    1315     178

  Fragments arranged by size: 

                1315     325     200     200     178      71      24      24
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     738     943    1496    1514    1581    2337 
   Size:        738     205     553      18      67     756

  Fragments arranged by size: 

                 756     738     553     205      67      18
 
TatI w'GTAC_w

Cuts at:      0    1142    2141    2337 
   Size:       1142     999     196

  Fragments arranged by size: 

                1142     999     196
 
Tth111I GACn'n_nGTC

Cuts at:      0    1888    2337 
   Size:       1888     449
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1929    2337 
   Size:       1929     408
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI    AflII   AflIII     AleI     AlfI    AlwNI
     ApoI     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI     BglI    BglII Bme1580I     BmrI     BmtI     BplI     BpmI
   Bpu10I     BsaI    BsaAI    BsaHI    BseRI    BseYI     BsgI  BsiHKAI
 Bsp1286I    BspMI    BsrBI    BsrFI    BssSI   BstAPI   BstEII    BstXI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI     EaeI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I     FspI    HaeII    Hin4I     KasI     KpnI
     MmeI     MscI     MslI   MspA1I     NarI     NcoI     NheI     NruI
     NspI     PasI    PflMI     PfoI     PmlI     PpiI    PpuMI     PsrI
     PstI    PvuII     SacI     ScaI     SfcI     SfiI     SfoI     SmlI
     SphI     StuI     StyI    TaqII     TatI  Tth111I     XcmI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI     AgeI     AhdI     AloI     ApaI
    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BciVI     BclI
    BfrBI     BlpI    BmgBI    BpuEI    BsaBI    BsaWI    BsaXI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrDI    BsrGI   BssHII
    BstBI  BstZ17I     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EarI    EcoRI    EcoRV     FalI     FseI    FspAI   HincII  HindIII
     HpaI     MfeI     MluI     NaeI     NdeI   NgoMIV     NotI     NsiI
     PacI     PciI     PmeI    PshAI     PsiI   PspOMI    PspXI     PvuI
    RsrII    SacII     SalI    SanDI     SapI     SbfI    SexAI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI     SwaI     TstI
     XbaI     XhoI     XmaI     XmnI     ZraI
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