################################## # NO CUT ENZYMES ( 60 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AhdI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BbrPI BcgI BfrI BlnI BsaBI BseAI BsiWI BspDI BspEI BspHI Bst1107I BstBI BstXI BstZ17I ClaI CpoI Eam1105I FseI FspI KpnI MamI MroI NdeI NheI NotI NruI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SexAI SfiI SfuI SgrAI SnaBI SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AccI 3332 GTCGAC GT^[AC][GT]AC AgeI 1856 ACCGGT A^CCGGT AocI 1382 CCTCAGG CC^T[ACGT]AGG Aor51HI 1268 AGCGCT AGC^GCT BamHI 2860 GGATCC G^GATCC BbsI 370 GAAGAC GAAGAC(2/6) BbvCI 5361 GCTGAGG CCTCAGC(-5/-2) BpuAI 370 GAAGAC GAAGAC(2/6) BsaAI 3169 TACGTG [CT]AC^GT[GA] BsmI 3606 GAATGC GAATGC(1/-1) BspLU11I 1590 ACATGT A^CATGT BsrBI 1158 GAGCGG CCGCTC(-3/-3) BstEII 2494 GGTAACC G^GT[ACGT]ACC BstPI 2494 GGTAACC G^GT[ACGT]ACC Bsu36I 1382 CCTCAGG CC^T[ACGT]AGG DrdI 340 GACAGAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1268 AGCGCT AGC^GCT Eco81I 1382 CCTCAGG CC^T[ACGT]AGG EcoO65I 2494 GGTAACC G^GT[ACGT]ACC EcoRV 4095 GATATC GAT^ATC HindIII 580 AAGCTT A^AGCTT MfeI 1049 CAATTG C^AATTG MluI 4421 ACGCGT A^CGCGT MunI 1049 CAATTG C^AATTG NaeI 4948 GCCGGC GCC^GGC NgoMIV 4948 GCCGGC G^CCGGC PflMI 2073 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 1856 ACCGGT A^CCGGT SalI 3332 GTCGAC G^TCGAC SpeI 2806 ACTAGT A^CTAGT StuI 4996 AGGCCT AGG^CCT Van91I 2073 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 2967 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### Alw44I 1850 GTGCAC G^TGCAC 3099 GTGCAC ApaI 4408 GGGCCC GGGCC^C 4879 GGGCCC ApaLI 1850 GTGCAC G^TGCAC 3099 GTGCAC BciVI 3229 GGATAC GTATCC(6/5) 5275 GGATAC BclI 756 TGATCA T^GATCA 2822 TGATCA BglII 1286 AGATCT A^GATCT 3215 AGATCT BsaWI 1705 ACCGGA [AT]^CCGG[AT] 1856 ACCGGT BsmBI 267 CGTCTC CGTCTC(1/5) 3869 CGTCTC BssSI 2437 CACGAG CACGAG(-5/-1) 847 CTCGTG BstAPI 2482 GCATATTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 5448 GCAGATCCTGC DraI 2155 TTTAAA TTT^AAA 2887 TTTAAA DraIII 844 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG 3832 CACTCGGTG EcoRI 2268 GAATTC G^AATTC 5192 GAATTC EcoT22I 826 ATGCAT ATGCA^T 1886 ATGCAT FbaI 756 TGATCA T^GATCA 2822 TGATCA HpaI 603 GTTAAC GTT^AAC 1321 GTTAAC NsiI 826 ATGCAT ATGCA^T 1886 ATGCAT PshAI 2900 GACATAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 4491 GACCCCCGTC SapI 979 GAAGAGC GCTCTTC(1/4) 1449 GCTCTTC SbfI 1544 CCTGCAGG CCTGCA^GG 5391 CCTGCAGG ScaI 761 AGTACT AGT^ACT 4622 AGTACT Sse8387I 1544 CCTGCAGG CCTGCA^GG 5391 CCTGCAGG ######################################### # 3 OR MORE CUT ENZYMES ( 143 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI ApoI Asp700I AspHI AvaI AvaII BalI BanI BanII BbeI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrDI BsrFI BsrGI BsrI BssHII BssKI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EarI EclXI Eco52I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KasI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI SacI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI SmlI SphI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmaCI XmaI XmnI