Restriction map for pF1KSDA0842
 (Linear) (Six-base) MAPSORT of: KIAA0842.seq  Check: 8612  from: 1  to: 3057



KIAA0842.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:29  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1784    3057 
   Size:       1784    1273
 
AatII G_ACGT'C

Cuts at:      0    2438    3057 
   Size:       2438     619
 
AccI GT'mk_AC

Cuts at:      0    1763    1904    3057 
   Size:       1763     141    1153

  Fragments arranged by size: 

                1763    1153     141
 
AfeI AGC'GCT

Cuts at:      0    1162    1213    1692    3057 
   Size:       1162      51     479    1365

  Fragments arranged by size: 

                1365    1162     479      51
 
AflIII A'CryG_T

Cuts at:      0    1796    3057 
   Size:       1796    1261
 
AhdI GACnn_n'nnGTC

Cuts at:      0     573    1980    3057 
   Size:        573    1407    1077

  Fragments arranged by size: 

                1407    1077     573
 
AleI CACnn'nnGTG

Cuts at:      0     859    3057 
   Size:        859    2198
 
AlwNI CAG_nnn'CTG

Cuts at:      0     132     522     835     900    1227    1274    1578    1630 
   Size:        132     390     313      65     327      47     304      52

Cuts at:   1630    1875    2043    2654    3002    3023    3057 
   Size:        245     168     611     348      21      34

  Fragments arranged by size: 

                 611     390     348     327     313     304     245     168
                 132      65      52      47      34      21
 
ApaI G_GGCC'C

Cuts at:      0    2278    3057 
   Size:       2278     779
 
ApaLI G'TGCA_C

Cuts at:      0     862    2019    2242    3057 
   Size:        862    1157     223     815

  Fragments arranged by size: 

                1157     862     815     223
 
ApoI r'AATT_y

Cuts at:      0    2179    2968    3057 
   Size:       2179     789      89

  Fragments arranged by size: 

                2179     789      89
 
AvaI C'yCGr_G

Cuts at:      0     928    1324    2302    3041    3057 
   Size:        928     396     978     739      16

  Fragments arranged by size: 

                 978     928     739     396      16
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     502     535    3057 
   Size:        502      33    2522

  Fragments arranged by size: 

                2522     502      33
 
BamHI G'GATC_C

Cuts at:      0      26    2405    3057 
   Size:         26    2379     652

  Fragments arranged by size: 

                2379     652      26
 
BanI G'GyrC_C

Cuts at:      0    2308    2347    3057 
   Size:       2308      39     710

  Fragments arranged by size: 

                2308     710      39
 
BanII G_rGCy'C

Cuts at:      0     793     929    1295    1729    2278    3057 
   Size:        793     136     366     434     549     779

  Fragments arranged by size: 

                 793     779     549     434     366     136
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     542    2383    3057 
   Size:        542    1841     674

  Fragments arranged by size: 

                1841     674     542
 
BbvCI CC'TCA_GC

Cuts at:      0    1694    3057 
   Size:       1694    1363
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     196     230     909     943    1221    1255    3057 
   Size:        196      34     679      34     278      34    1802

  Fragments arranged by size: 

                1802     679     278     196      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1548    3057 
   Size:       1548    1509
 
BclI T'GATC_A

Cuts at:      0    2105    3057 
   Size:       2105     952
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1064    2581    3057 
   Size:       1064    1517     476

  Fragments arranged by size: 

                1517    1064     476
 
BlpI GC'TnA_GC

Cuts at:      0    1289    2395    3057 
   Size:       1289    1106     662

  Fragments arranged by size: 

                1289    1106     662
 
Bme1580I G_kGCm'C

Cuts at:      0     866    1853    2023    2246    2278    2311    2350    2743 
   Size:        866     987     170     223      32      33      39     393

Cuts at:   2743    3057 
   Size:        314

  Fragments arranged by size: 

                 987     866     393     314     223     170      39      33
                  32
 
BmgBI CAC'GTC

Cuts at:      0    2381    3057 
   Size:       2381     676
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     201     714    2256    3057 
   Size:        201     513    1542     801

  Fragments arranged by size: 

                1542     801     513     201
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      57    1586    3057 
   Size:         57    1529    1471

  Fragments arranged by size: 

                1529    1471      57
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     634    1600    2572    3057 
   Size:         52     582     966     972     485

  Fragments arranged by size: 

                 972     966     582     485      52
 
Bpu10I CC'TnA_GC

Cuts at:      0    1228    1571    1694    2854    3057 
   Size:       1228     343     123    1160     203

  Fragments arranged by size: 

                1228    1160     343     203     123
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1642    3003    3057 
   Size:       1642    1361      54

  Fragments arranged by size: 

                1642    1361      54
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     710    1658    3038    3057 
   Size:        710     948    1380      19

  Fragments arranged by size: 

                1380     948     710      19
 
BsaAI yAC'GTr

Cuts at:      0     255    1797    1881    2699    3057 
   Size:        255    1542      84     818     358

  Fragments arranged by size: 

                1542     818     358     255      84
 
BsaHI Gr'CG_yC

Cuts at:      0    1537    2435    3057 
   Size:       1537     898     622

  Fragments arranged by size: 

                1537     898     622
 
BsaWI w'CCGG_w

Cuts at:      0      23     104    1835    1913    3057 
   Size:         23      81    1731      78    1144

  Fragments arranged by size: 

                1731    1144      81      78      23
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     295     325    1305    1335    3003    3033    3057 
   Size:        295      30     980      30    1668      30      24

  Fragments arranged by size: 

                1668     980     295      30      30      30      24
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     171     834     835     901    1006    1135    1532    1535 
   Size:        171     663       1      66     105     129     397       3

Cuts at:   1535    1538    2821    3057 
   Size:          3    1283     236

  Fragments arranged by size: 

                1283     663     397     236     171     129     105      66
                   3       3       1
 
BseYI C'CCAG_C

Cuts at:      0     547     694     797    1267    1586    1672    2134    2647 
   Size:        547     147     103     470     319      86     462     513

Cuts at:   2647    2751    3057 
   Size:        104     306

  Fragments arranged by size: 

                 547     513     470     462     319     306     147     104
                 103      86
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     903    2317    2844    2873    3057 
   Size:        903    1414     527      29     184

  Fragments arranged by size: 

                1414     903     527     184      29
 
BsiEI CG_ry'CG

Cuts at:      0    2541    3033    3057 
   Size:       2541     492      24

  Fragments arranged by size: 

                2541     492      24
 
BsiHKAI G_wGCw'C

Cuts at:      0     143     200     866     929    1282    1846    2023    2246 
   Size:        143      57     666      63     353     564     177     223

Cuts at:   2246    2396    3057 
   Size:        150     661

  Fragments arranged by size: 

                 666     661     564     353     223     177     150     143
                  63      57
 
BsmI GAATG_Cn'

Cuts at:      0     384    3057 
   Size:        384    2673
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2775    3057 
   Size:       2775     282
 
Bsp1286I G_dGCh'C

Cuts at:      0     143     200     793     866     929    1282    1295    1729 
   Size:        143      57     593      73      63     353      13     434

Cuts at:   1729    1846    1853    2023    2246    2278    2311    2350    2396 
   Size:        117       7     170     223      32      33      39      46

Cuts at:   2396    2743    3057 
   Size:        347     314

  Fragments arranged by size: 

                 593     434     353     347     314     223     170     143
                 117      73      63      57      46      39      33      32
                  13       7
 
BspEI T'CCGG_A

Cuts at:      0     104    1835    1913    3057 
   Size:        104    1731      78    1144

  Fragments arranged by size: 

                1731    1144     104      78
 
BspHI T'CATG_A

Cuts at:      0    1787    3057 
   Size:       1787    1270
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     335     530    1784    1874    1916    3057 
   Size:        335     195    1254      90      42    1141

  Fragments arranged by size: 

                1254    1141     335     195      90      42
 
BsrBI CCG'CTC

Cuts at:      0     746    2732    3057 
   Size:        746    1986     325

  Fragments arranged by size: 

                1986     746     325
 
BsrFI r'CCGG_y

Cuts at:      0     825    1315    2540    3057 
   Size:        825     490    1225     517

  Fragments arranged by size: 

                1225     825     517     490
 
BssSI C'ACGA_G

Cuts at:      0     199    1560    3057 
   Size:        199    1361    1497

  Fragments arranged by size: 

                1497    1361     199
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1277    2043    2663    3057 
   Size:       1277     766     620     394

  Fragments arranged by size: 

                1277     766     620     394
 
BstEII G'GTnAC_C

Cuts at:      0     942    3057 
   Size:        942    2115
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     267     612    2184    3057 
   Size:        267     345    1572     873

  Fragments arranged by size: 

                1572     873     345     267
 
BstYI r'GATC_y

Cuts at:      0      26      92     459    2405    3057 
   Size:         26      66     367    1946     652

  Fragments arranged by size: 

                1946     652     367      66      26
 
Bsu36I CC'TnA_GG

Cuts at:      0    1490    3057 
   Size:       1490    1567
 
BtgI C'CryG_G

Cuts at:      0     558    1145    1205    2156    2501    3027    3057 
   Size:        558     587      60     951     345     526      30

  Fragments arranged by size: 

                 951     587     558     526     345      60      30
 
BtsI GCAGTG_nn'

Cuts at:      0     686    2289    2479    3057 
   Size:        686    1603     190     578

  Fragments arranged by size: 

                1603     686     578     190
 
DraIII CAC_nnn'GTG

Cuts at:      0    1277    3057 
   Size:       1277    1780
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     426    1900    3057 
   Size:        426    1474    1157

  Fragments arranged by size: 

                1474    1157     426
 
EaeI y'GGCC_r

Cuts at:      0     257    1437    1461    1500    2174    2582    3030    3057 
   Size:        257    1180      24      39     674     408     448      27

  Fragments arranged by size: 

                1180     674     448     408     257      39      27      24
            
 
EagI C'GGCC_G

Cuts at:      0    3030    3057 
   Size:       3030      27
 
EarI CTCTTCn'nnn_

Cuts at:      0     418    1505    1808    1943    3057 
   Size:        418    1087     303     135    1114

  Fragments arranged by size: 

                1114    1087     418     303     135
 
EcoICRI GAG'CTC

Cuts at:      0     927    3057 
   Size:        927    2130
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     634    1600    2572    3057 
   Size:         52     582     966     972     485

  Fragments arranged by size: 

                 972     966     582     485      52
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     783    1231    2362    3057 
   Size:        783     448    1131     695

  Fragments arranged by size: 

                1131     783     695     448
 
EcoO109I rG'GnC_Cy

Cuts at:      0     120     897    1609    1634    2253    2275    3057 
   Size:        120     777     712      25     619      22     782

  Fragments arranged by size: 

                 782     777     712     619     120      25      22
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     559    2400    3057 
   Size:        559    1841     657

  Fragments arranged by size: 

                1841     657     559
 
HaeII r_GCGC'y

Cuts at:      0     804    1164    1215    1694    2068    2768    3002    3057 
   Size:        804     360      51     479     374     700     234      55

  Fragments arranged by size: 

                 804     700     479     374     360     234      55      51
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     166     198     212     244     559     591     823     855 
   Size:        166      32      14      32     315      32     232      32

Cuts at:    855    1305    1337    1966    1998    2583    2615    3003    3035 
   Size:        450      32     629      32     585      32     388      32

Cuts at:   3035    3057 
   Size:         22

  Fragments arranged by size: 

                 629     585     450     388     315     232     166      32
                  32      32      32      32      32      32      32      22
                  14
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     782    2561    2981    3057 
   Size:        782    1779     420      76

  Fragments arranged by size: 

                1779     782     420      76
 
MscI TGG'CCA

Cuts at:      0     259    1463    1502    2176    3057 
   Size:        259    1204      39     674     881

  Fragments arranged by size: 

                1204     881     674     259      39
 
MslI CAynn'nnrTG

Cuts at:      0     859    1204    3057 
   Size:        859     345    1853

  Fragments arranged by size: 

                1853     859     345
 
MspA1I CmG'CkG

Cuts at:      0    1260    1470    1590    1821    1872    3009    3029    3057 
   Size:       1260     210     120     231      51    1137      20      28

  Fragments arranged by size: 

                1260    1137     231     210     120      51      28      20
            
 
NaeI GCC'GGC

Cuts at:      0     827    3057 
   Size:        827    2230
 
NcoI C'CATG_G

Cuts at:      0    1205    2156    3057 
   Size:       1205     951     901

  Fragments arranged by size: 

                1205     951     901
 
NgoMIV G'CCGG_C

Cuts at:      0     825    3057 
   Size:        825    2232
 
NspI r_CATG'y

Cuts at:      0      83     498    1477    2331    3057 
   Size:         83     415     979     854     726

  Fragments arranged by size: 

                 979     854     726     415      83
 
PasI CC'CwG_GG

Cuts at:      0    2271    2634    2840    3057 
   Size:       2271     363     206     217

  Fragments arranged by size: 

                2271     363     217     206
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     900    1078    1274    1630    3057 
   Size:        900     178     196     356    1427

  Fragments arranged by size: 

                1427     900     356     196     178
 
PfoI T'CCnGG_A

Cuts at:      0      29     338    2945    3057 
   Size:         29     309    2607     112

  Fragments arranged by size: 

                2607     309     112      29
 
PmlI CAC'GTG

Cuts at:      0     255    1797    3057 
   Size:        255    1542    1260

  Fragments arranged by size: 

                1542    1260     255
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1807    1839    3057 
   Size:       1807      32    1218

  Fragments arranged by size: 

                1807    1218      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     120    1634    2253    3057 
   Size:        120    1514     619     804

  Fragments arranged by size: 

                1514     804     619     120
 
PspOMI G'GGCC_C

Cuts at:      0    2274    3057 
   Size:       2274     783
 
PspXI vC'TCGA_Gb

Cuts at:      0     928    3057 
   Size:        928    2129
 
PstI C_TGCA'G

Cuts at:      0     251     394    1021    1455    1871    3057 
   Size:        251     143     627     434     416    1186

  Fragments arranged by size: 

                1186     627     434     416     251     143
 
PvuII CAG'CTG

Cuts at:      0    1260    1590    1821    1872    3057 
   Size:       1260     330     231      51    1185

  Fragments arranged by size: 

                1260    1185     330     231      51
 
RsrII CG'GwC_CG

Cuts at:      0    1313    2424    3057 
   Size:       1313    1111     633

  Fragments arranged by size: 

                1313    1111     633
 
SacI G_AGCT'C

Cuts at:      0     929    3057 
   Size:        929    2128
 
SacII CC_GC'GG

Cuts at:      0    3030    3057 
   Size:       3030      27
 
SanDI GG'GwC_CC

Cuts at:      0    1634    3057 
   Size:       1634    1423
 
SapI GCTCTTCn'nnn_

Cuts at:      0     418    1808    3057 
   Size:        418    1390    1249

  Fragments arranged by size: 

                1390    1249     418
 
SbfI CC_TGCA'GG

Cuts at:      0    1455    3057 
   Size:       1455    1602
 
ScaI AGT'ACT

Cuts at:      0     367    2794    3057 
   Size:        367    2427     263

  Fragments arranged by size: 

                2427     367     263
 
SexAI A'CCwGG_T

Cuts at:      0    1273    1940    3057 
   Size:       1273     667    1117

  Fragments arranged by size: 

                1273    1117     667
 
SfcI C'TryA_G

Cuts at:      0     124     247     390    1017    1451    1867    3057 
   Size:        124     123     143     627     434     416    1190

  Fragments arranged by size: 

                1190     627     434     416     143     124     123
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    2581    3057 
   Size:       2581     476
 
SmaI CCC'GGG

Cuts at:      0    1326    3057 
   Size:       1326    1731
 
SmlI C'TyrA_G

Cuts at:      0     928    1657    2982    3057 
   Size:        928     729    1325      75

  Fragments arranged by size: 

                1325     928     729      75
 
SphI G_CATG'C

Cuts at:      0    2331    3057 
   Size:       2331     726
 
StuI AGG'CCT

Cuts at:      0     778    3057 
   Size:        778    2279
 
StyI C'CwwG_G

Cuts at:      0     314     422    1077    1205    2156    3057 
   Size:        314     108     655     128     951     901

  Fragments arranged by size: 

                 951     901     655     314     128     108
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     183     543     811    2891    3057 
   Size:        183     360     268    2080     166

  Fragments arranged by size: 

                2080     360     268     183     166
 
TatI w'GTAC_w

Cuts at:      0     365    2792    3057 
   Size:        365    2427     265

  Fragments arranged by size: 

                2427     365     265
 
Tth111I GACn'n_nGTC

Cuts at:      0     118     730     837    3057 
   Size:        118     612     107    2220

  Fragments arranged by size: 

                2220     612     118     107
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1212    2163    2919    3057 
   Size:       1212     951     756     138

  Fragments arranged by size: 

                1212     951     756     138
 
XhoI C'TCGA_G

Cuts at:      0     928    3057 
   Size:        928    2129
 
XmaI C'CCGG_G

Cuts at:      0    1324    3057 
   Size:       1324    1733
 
XmnI GAAnn'nnTTC

Cuts at:      0     811    2968    3057 
   Size:        811    2157      89

  Fragments arranged by size: 

                2157     811      89
 
ZraI GAC'GTC

Cuts at:      0    2436    3057 
   Size:       2436     621
 

 Enzymes that do cut:

     AarI    AatII     AccI     AfeI   AflIII     AhdI     AleI    AlwNI
     ApaI    ApaLI     ApoI     AvaI     BaeI    BamHI     BanI    BanII
     BbsI    BbvCI     BcgI    BciVI     BclI     BglI     BlpI Bme1580I
    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrFI
    BssSI   BstAPI   BstEII    BstXI    BstYI   Bsu36I     BtgI     BtsI
   DraIII     DrdI     EaeI     EagI     EarI  EcoICRI  Eco57MI    EcoNI
 EcoO109I     FalI    HaeII    Hin4I     MmeI     MscI     MslI   MspA1I
     NaeI     NcoI   NgoMIV     NspI     PasI    PflMI     PfoI     PmlI
     PpiI    PpuMI   PspOMI    PspXI     PstI    PvuII    RsrII     SacI
    SacII    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SmaI     SmlI     SphI     StuI     StyI    TaqII     TatI  Tth111I
     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AcuI    AflII     AgeI     AlfI     AloI     AscI
     AseI    AsiSI    AvrII     BbeI    BfrBI    BglII     BmtI    BsaBI
    BsiWI    BsrDI    BsrGI   BssHII    BstBI  BstZ17I    BtgZI     ClaI
    CspCI     DraI     EciI    EcoRI    EcoRV     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     NarI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PciI     PmeI
    PshAI     PsiI     PsrI     PvuI     SalI     SfoI     SgfI    SgrAI
    SnaBI     SpeI     SrfI     SspI     SwaI     TstI     XbaI
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