Restriction map for
pF1KSDA0742
(Circular) (Six-base) MAPSORT of: pF1KSDA0742.seq Check: 9942 from: 1 to: 7102
pF1KSDA0742 7102 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1486 1486
Size: 7102
AccI GT'mk_AC
Cuts at: 365 3137 4138 365
Size: 2772 1001 3329
Fragments arranged by size:
3329 2772 1001
Acc65I G'GTAC_C
Cuts at: 4116 4116
Size: 7102
AclI AA'CG_TT
Cuts at: 1952 6942 1952
Size: 4990 2112
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 1208 1532 1571 1827 3093 3473 3929 4925
Size: 880 324 39 256 1266 380 456 996
Cuts at: 4925 5357 6106 6374 328
Size: 432 749 268 1056
Fragments arranged by size:
1266 1056 996 880 749 456 432 380
324 268 256 39
AflII C'TTAA_G
Cuts at: 3259 3565 3259
Size: 306 6796
AflIII A'CryG_T
Cuts at: 874 4452 5558 6535 874
Size: 3578 1106 977 1441
Fragments arranged by size:
3578 1441 1106 977
AgeI A'CCGG_T
Cuts at: 5490 5490
Size: 7102
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 975 3671 975
Size: 2696 4406
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2321 2353 2321
Size: 32 7070
AlwNI CAG_nnn'CTG
Cuts at: 2515 4405 5974 2515
Size: 1890 1569 3643
Fragments arranged by size:
3643 1890 1569
ApaI G_GGCC'C
Cuts at: 3418 3418
Size: 7102
ApaLI G'TGCA_C
Cuts at: 5872 5872
Size: 7102
ApoI r'AATT_y
Cuts at: 322 434 631 713 1121 1160 1268 1572 2793
Size: 112 197 82 408 39 108 304 1221
Cuts at: 2793 3369 3527 3685 3961 4104 6455 6711 322
Size: 576 158 158 276 143 2351 256 713
Fragments arranged by size:
2351 1221 713 576 408 304 276 256
197 158 158 143 112 108 82 39
AseI AT'TA_AT
Cuts at: 20 3442 20
Size: 3422 3680
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7102
AvaI C'yCGr_G
Cuts at: 3340 4120 3340
Size: 780 6322
BamHI G'GATC_C
Cuts at: 502 4125 502
Size: 3623 3479
BanI G'GyrC_C
Cuts at: 4116 4530 4778 4813 4116
Size: 414 248 35 6405
Fragments arranged by size:
6405 414 248 35
BanII G_rGCy'C
Cuts at: 3418 3867 4114 5144 3418
Size: 449 247 1030 5376
Fragments arranged by size:
5376 1030 449 247
BbeI G_GCGC'C
Cuts at: 4534 4782 4534
Size: 248 6854
BbsI GAAGACnn'nnnn_
Cuts at: 583 908 2435 3781 583
Size: 325 1527 1346 3904
Fragments arranged by size:
3904 1527 1346 325
BciVI GTATCCnnnnn_n'
Cuts at: 4998 5761 7102 4998
Size: 763 1341 4998
Fragments arranged by size:
4998 1341 763
BclI T'GATC_A
Cuts at: 1317 3750 4620 1317
Size: 2433 870 3799
Fragments arranged by size:
3799 2433 870
BglII A'GATC_T
Cuts at: 3408 4615 6654 3408
Size: 1207 2039 3856
Fragments arranged by size:
3856 2039 1207
BlpI GC'TnA_GC
Cuts at: 4212 4212
Size: 7102
Bme1580I G_kGCm'C
Cuts at: 3418 4725 4818 5876 3418
Size: 1307 93 1058 4644
Fragments arranged by size:
4644 1307 1058 93
BmgBI CAC'GTC
Cuts at: 228 228
Size: 7102
BmrI ACTGGGnnnn_n'
Cuts at: 971 2154 4312 4393 4728 6874 971
Size: 1183 2158 81 335 2146 1199
Fragments arranged by size:
2158 2146 1199 1183 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5467 5467
Size: 7102
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1277 2467 2474 3083 3908 1277
Size: 1190 7 609 825 4471
Fragments arranged by size:
4471 1190 825 609 7
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1112 2809 2891 4276 5155 5649 5947 1112
Size: 1697 82 1385 879 494 298 2267
Fragments arranged by size:
2267 1697 1385 879 494 298 82
BsaI GGTCTCn'nnnn_
Cuts at: 1997 6806 1997
Size: 4809 2293
BsaAI yAC'GTr
Cuts at: 4092 4453 4092
Size: 361 6741
BsaBI GATnn'nnATC
Cuts at: 6 4614 6653 6
Size: 4608 2039 455
Fragments arranged by size:
4608 2039 455
BsaHI Gr'CG_yC
Cuts at: 4531 4779 5483 6979 4531
Size: 248 704 1496 4654
Fragments arranged by size:
4654 1496 704 248
BsaWI w'CCGG_w
Cuts at: 2189 2780 4289 4810 5490 5764 5911 6322 2189
Size: 591 1509 521 680 274 147 411 2969
Fragments arranged by size:
2969 1509 680 591 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2162 2192 2321 2351 3167 3197 2162
Size: 30 129 30 816 30 6067
Fragments arranged by size:
6067 816 129 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 183 817 2573 2939 183
Size: 634 1756 366 4346
Fragments arranged by size:
4346 1756 634 366
BseYI C'CCAG_C
Cuts at: 221 771 1674 2926 3726 3815 4525 5283 5862
Size: 550 903 1252 800 89 710 758 579
Cuts at: 5862 6358 221
Size: 496 965
Fragments arranged by size:
1252 965 903 800 758 710 579 550
496 89
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1012 2125 2495 1012
Size: 1113 370 5619
Fragments arranged by size:
5619 1113 370
BsiEI CG_ry'CG
Cuts at: 104 4642 4688 5898 104
Size: 4538 46 1210 1308
Fragments arranged by size:
4538 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 134 2483 3642 3867 4114 4892 5082 5876 134
Size: 2349 1159 225 247 778 190 794 1360
Fragments arranged by size:
2349 1360 1159 794 778 247 225 190
BsmI GAATG_Cn'
Cuts at: 1647 2930 1647
Size: 1283 5819
Bsp1286I G_dGCh'C
Cuts at: 134 2483 3418 3642 3867 4114 4725 4818 4892
Size: 2349 935 224 225 247 611 93 74
Cuts at: 4892 5082 5144 5876 134
Size: 190 62 732 1360
Fragments arranged by size:
2349 1360 935 732 611 247 225 224
190 93 74 62
BspEI T'CCGG_A
Cuts at: 2780 6322 2780
Size: 3542 3560
BspHI T'CATG_A
Cuts at: 3747 6278 3747
Size: 2531 4571
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1486 2147 4150 4364 4666 5047 1486
Size: 661 2003 214 302 381 3541
Fragments arranged by size:
3541 2003 661 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5392 5446 6932 7097 50
Size: 5342 54 1486 165 55
Fragments arranged by size:
5342 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 5012 5012
Size: 7102
BsrFI r'CCGG_y
Cuts at: 153 2545 5098 5279 5490 153
Size: 2392 2553 181 211 1765
Fragments arranged by size:
2553 2392 1765 211 181
BsrGI T'GTAC_A
Cuts at: 450 855 3825 450
Size: 405 2970 3727
Fragments arranged by size:
3727 2970 405
BssSI C'ACGA_G
Cuts at: 5371 5731 5371
Size: 360 6742
BstAPI GCAn_nnn'nTGC
Cuts at: 4477 4477
Size: 7102
BstBI TT'CG_AA
Cuts at: 110 110
Size: 7102
BstEII G'GTnAC_C
Cuts at: 1934 1934
Size: 7102
BstXI CCAn_nnnn'nTGG
Cuts at: 2934 2934
Size: 7102
BstYI r'GATC_y
Cuts at: 1 303 502 3408 4125 4592 4615 4950 5196
Size: 302 199 2906 717 467 23 335 246
Cuts at: 5196 6210 6296 6308 6654 1
Size: 1014 86 12 346 449
Fragments arranged by size:
2906 1014 717 467 449 346 335 302
246 199 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1367 1367
Size: 7102
BtgI C'CryG_G
Cuts at: 125 125
Size: 7102
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 218 5050 5231 5394 218
Size: 4832 181 163 1926
Fragments arranged by size:
4832 1926 181 163
BtsI GCAGTG_nn'
Cuts at: 2483 3334 3939 4040 4343 2483
Size: 851 605 101 303 5242
Fragments arranged by size:
5242 851 605 303 101
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1334 1369 1334
Size: 35 7067
DraI TTT'AAA
Cuts at: 677 1577 2663 4099 677
Size: 900 1086 1436 3680
Fragments arranged by size:
3680 1436 1086 900
DraIII CAC_nnn'GTG
Cuts at: 2148 2148
Size: 7102
DrdI GACnn_nn'nnGTC
Cuts at: 4806 5666 4806
Size: 860 6242
EaeI y'GGCC_r
Cuts at: 151 1991 2597 3268 3323 3403 4685 4859 5250
Size: 1840 606 671 55 80 1282 174 391
Cuts at: 5250 5277 6624 151
Size: 27 1347 629
Fragments arranged by size:
1840 1347 1282 671 629 606 391 174
80 55 27
EagI C'GGCC_G
Cuts at: 4685 4685
Size: 7102
EarI CTCTTCn'nnn_
Cuts at: 791 2191 2251 3701 5123 5333 791
Size: 1400 60 1450 1422 210 2560
Fragments arranged by size:
2560 1450 1422 1400 210 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5308 5620 5766 6912 5308
Size: 312 146 1146 5498
Fragments arranged by size:
5498 1146 312 146
EcoICRI GAG'CTC
Cuts at: 3865 4112 3865
Size: 247 6855
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 1208 1277 1532 1571 1827 2467 2474 3083
Size: 880 69 255 39 256 640 7 609
Cuts at: 3083 3093 3473 3908 3929 4925 5357 6106 6374
Size: 10 380 435 21 996 432 749 268
Cuts at: 6374 328
Size: 1056
Fragments arranged by size:
1056 996 880 749 640 609 435 432
380 268 256 255 69 39 21 10
7
EcoNI CCTnn'n_nnAGG
Cuts at: 1366 1366
Size: 7102
EcoO109I rG'GnC_Cy
Cuts at: 1062 3415 4239 1062
Size: 2353 824 3925
Fragments arranged by size:
3925 2353 824
EcoRI G'AATT_C
Cuts at: 2793 3527 3961 4104 2793
Size: 734 434 143 5791
Fragments arranged by size:
5791 734 434 143
EcoRV GAT'ATC
Cuts at: 3970 3970
Size: 7102
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1392 2004 3580 5119 1392
Size: 612 1576 1539 3375
Fragments arranged by size:
3375 1576 1539 612
FspI TGC'GCA
Cuts at: 4881 4881
Size: 7102
HaeII r_GCGC'y
Cuts at: 4534 4782 5806 6785 4534
Size: 248 1024 979 4851
Fragments arranged by size:
4851 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 319 351 2903 2935 4942 4972 4974 5004 319
Size: 32 2552 32 2007 30 2 30 2417
Fragments arranged by size:
2552 2417 2007 32 32 30 30 2
HincII GTy'rAC
Cuts at: 795 2048 2622 4139 795
Size: 1253 574 1517 3758
Fragments arranged by size:
3758 1517 1253 574
HindIII A'AGCT_T
Cuts at: 638 638
Size: 7102
KasI G'GCGC_C
Cuts at: 4530 4778 4530
Size: 248 6854
KpnI G_GTAC'C
Cuts at: 4120 4120
Size: 7102
MfeI C'AATT_G
Cuts at: 1922 1922
Size: 7102
MluI A'CGCG_T
Cuts at: 6535 6535
Size: 7102
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 464 2307 2483 5773 5957 464
Size: 1843 176 3290 184 1609
Fragments arranged by size:
3290 1843 1609 184 176
MscI TGG'CCA
Cuts at: 1993 3270 3325 4861 1993
Size: 1277 55 1536 4234
Fragments arranged by size:
4234 1536 1277 55
MslI CAynn'nnrTG
Cuts at: 659 3488 3653 3901 5089 5216 659
Size: 2829 165 248 1188 127 2545
Fragments arranged by size:
2829 2545 1188 248 165 127
MspA1I CmG'CkG
Cuts at: 221 1292 2515 2612 4211 4525 4885 5900 6145
Size: 1071 1223 97 1599 314 360 1015 245
Cuts at: 6145 221
Size: 1178
Fragments arranged by size:
1599 1223 1178 1071 1015 360 314 245
97
NaeI GCC'GGC
Cuts at: 2547 5281 2547
Size: 2734 4368
NarI GG'CG_CC
Cuts at: 4531 4779 4531
Size: 248 6854
NcoI C'CATG_G
Cuts at: 125 125
Size: 7102
NgoMIV G'CCGG_C
Cuts at: 2545 5279 2545
Size: 2734 4368
NruI TCG'CGA
Cuts at: 5463 5463
Size: 7102
NspI r_CATG'y
Cuts at: 878 1453 3934 4153 4653 5562 878
Size: 575 2481 219 500 909 2418
Fragments arranged by size:
2481 2418 909 575 500 219
PciI A'CATG_T
Cuts at: 874 5558 874
Size: 4684 2418
PfoI T'CCnGG_A
Cuts at: 259 1651 2304 259
Size: 1392 653 5057
Fragments arranged by size:
5057 1392 653
PmeI GTTT'AAAC
Cuts at: 4099 4099
Size: 7102
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2321 2353 6241 6273 2321
Size: 32 3888 32 3150
Fragments arranged by size:
3888 3150 32 32
PpuMI rG'GwC_Cy
Cuts at: 1062 1062
Size: 7102
PspOMI G'GGCC_C
Cuts at: 3414 3414
Size: 7102
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 434 466 434
Size: 32 7070
PstI C_TGCA'G
Cuts at: 1044 1436 4147 4832 1044
Size: 392 2711 685 3314
Fragments arranged by size:
3314 2711 685 392
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7102
PvuII CAG'CTG
Cuts at: 221 1292 2515 4525 4885 221
Size: 1071 1223 2010 360 2438
Fragments arranged by size:
2438 2010 1223 1071 360
RsrII CG'GwC_CG
Cuts at: 5295 5295
Size: 7102
SacI G_AGCT'C
Cuts at: 3867 4114 3867
Size: 247 6855
SalI G'TCGA_C
Cuts at: 4137 4137
Size: 7102
SapI GCTCTTCn'nnn_
Cuts at: 3701 5123 5333 3701
Size: 1422 210 5470
Fragments arranged by size:
5470 1422 210
SbfI CC_TGCA'GG
Cuts at: 4147 4147
Size: 7102
ScaI AGT'ACT
Cuts at: 1080 6619 1080
Size: 5539 1563
SfcI C'TryA_G
Cuts at: 33 366 1040 1432 2801 4143 4828 5823 6014
Size: 333 674 392 1369 1342 685 995 191
Cuts at: 6014 33
Size: 1121
Fragments arranged by size:
1369 1342 1121 995 685 674 392 333
191
SfoI GGC'GCC
Cuts at: 4532 4780 4532
Size: 248 6854
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7102
SmaI CCC'GGG
Cuts at: 4122 4122
Size: 7102
SmlI C'TyrA_G
Cuts at: 1127 2824 2906 3259 3565 4255 5170 5664 5926
Size: 1697 82 353 306 690 915 494 262
Cuts at: 5926 1127
Size: 2303
Fragments arranged by size:
2303 1697 915 690 494 353 306 262
82
SnaBI TAC'GTA
Cuts at: 4092 4092
Size: 7102
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7102
SphI G_CATG'C
Cuts at: 4153 4653 4153
Size: 500 6602
StuI AGG'CCT
Cuts at: 970 1711 2904 970
Size: 741 1193 5168
Fragments arranged by size:
5168 1193 741
StyI C'CwwG_G
Cuts at: 125 1110 4234 4588 125
Size: 985 3124 354 2639
Fragments arranged by size:
3124 2639 985 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2136 2509 3785 4683 4816 5275 5486 2136
Size: 373 1276 898 133 459 211 3752
Fragments arranged by size:
3752 1276 898 459 373 211 133
TatI w'GTAC_w
Cuts at: 450 855 1036 1078 1294 1982 3344 3825 6617
Size: 405 181 42 216 688 1362 481 2792
Cuts at: 6617 450
Size: 935
Fragments arranged by size:
2792 1362 935 688 481 405 216 181
42
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2162 2194 2825 2857 6619 6651 2162
Size: 32 631 32 3762 32 2613
Fragments arranged by size:
3762 2613 631 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4897 4897
Size: 7102
XbaI T'CTAG_A
Cuts at: 4131 4131
Size: 7102
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 737 1953 3641 4595 737
Size: 1216 1688 954 3244
Fragments arranged by size:
3244 1688 1216 954
XmaI C'CCGG_G
Cuts at: 4120 4120
Size: 7102
XmnI GAAnn'nnTTC
Cuts at: 3212 3212
Size: 7102
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NruI NspI
PciI PfoI PmeI PpiI PpuMI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AleI AscI AvrII BaeI BbvCI
BcgI BfrBI BglI BmtI Bpu10I BsiWI BsmBI BssHII
BstZ17I ClaI FseI FspAI HpaI NdeI NheI NotI
NsiI PacI PasI PflMI PmlI PshAI PsiI PspXI
SacII SanDI SexAI SfiI SgrAI SrfI SspI SwaI
XhoI ZraI