Restriction map for pF1KSDA0742
 (Linear) (Six-base) MAPSORT of: KIAA0742.seq  Check: 2824  from: 1  to: 3963



KIAA0742.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:28  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1360    3963 
   Size:       1360    2603
 
AccI GT'mk_AC

Cuts at:      0     239    3011    3963 
   Size:        239    2772     952

  Fragments arranged by size: 

                2772     952     239
 
AclI AA'CG_TT

Cuts at:      0    1826    3963 
   Size:       1826    2137
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     202    1082    1406    1445    1701    2967    3347    3803 
   Size:        202     880     324      39     256    1266     380     456

Cuts at:   3803    3963 
   Size:        160

  Fragments arranged by size: 

                1266     880     456     380     324     256     202     160
                  39
 
AflII C'TTAA_G

Cuts at:      0    3133    3439    3963 
   Size:       3133     306     524

  Fragments arranged by size: 

                3133     524     306
 
AflIII A'CryG_T

Cuts at:      0     748    3963 
   Size:        748    3215
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     849    3545    3963 
   Size:        849    2696     418

  Fragments arranged by size: 

                2696     849     418
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2195    2227    3963 
   Size:       2195      32    1736

  Fragments arranged by size: 

                2195    1736      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    2389    3963 
   Size:       2389    1574
 
ApaI G_GGCC'C

Cuts at:      0    3292    3963 
   Size:       3292     671
 
ApoI r'AATT_y

Cuts at:      0     196     308     505     587     995    1034    1142    1446 
   Size:        196     112     197      82     408      39     108     304

Cuts at:   1446    2667    3243    3401    3559    3835    3963 
   Size:       1221     576     158     158     276     128

  Fragments arranged by size: 

                1221     576     408     304     276     197     196     158
                 158     128     112     108      82      39
 
AseI AT'TA_AT

Cuts at:      0    3316    3963 
   Size:       3316     647
 
AvaI C'yCGr_G

Cuts at:      0    3214    3963 
   Size:       3214     749
 
BamHI G'GATC_C

Cuts at:      0     376    3963 
   Size:        376    3587
 
BanII G_rGCy'C

Cuts at:      0    3292    3741    3963 
   Size:       3292     449     222

  Fragments arranged by size: 

                3292     449     222
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     457     782    2309    3655    3963 
   Size:        457     325    1527    1346     308

  Fragments arranged by size: 

                1527    1346     457     325     308
 
BclI T'GATC_A

Cuts at:      0    1191    3624    3963 
   Size:       1191    2433     339

  Fragments arranged by size: 

                2433    1191     339
 
BglII A'GATC_T

Cuts at:      0    3282    3963 
   Size:       3282     681
 
Bme1580I G_kGCm'C

Cuts at:      0    3292    3963 
   Size:       3292     671
 
BmgBI CAC'GTC

Cuts at:      0     102    3963 
   Size:        102    3861
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     845    2028    3963 
   Size:        845    1183    1935

  Fragments arranged by size: 

                1935    1183     845
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1151    2341    2348    2957    3782    3963 
   Size:       1151    1190       7     609     825     181

  Fragments arranged by size: 

                1190    1151     825     609     181       7
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     986    2683    2765    3963 
   Size:        986    1697      82    1198

  Fragments arranged by size: 

                1697    1198     986      82
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1871    3963 
   Size:       1871    2092
 
BsaWI w'CCGG_w

Cuts at:      0    2063    2654    3963 
   Size:       2063     591    1309

  Fragments arranged by size: 

                2063    1309     591
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2036    2066    2195    2225    3041    3071    3963 
   Size:       2036      30     129      30     816      30     892

  Fragments arranged by size: 

                2036     892     816     129      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      57     691    2447    2813    3963 
   Size:         57     634    1756     366    1150

  Fragments arranged by size: 

                1756    1150     634     366      57
 
BseYI C'CCAG_C

Cuts at:      0      95     645    1548    2800    3600    3689    3963 
   Size:         95     550     903    1252     800      89     274

  Fragments arranged by size: 

                1252     903     800     550     274      95      89
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     886    1999    2369    3963 
   Size:        886    1113     370    1594

  Fragments arranged by size: 

                1594    1113     886     370
 
BsiHKAI G_wGCw'C

Cuts at:      0       8    2357    3516    3741    3963 
   Size:          8    2349    1159     225     222

  Fragments arranged by size: 

                2349    1159     225     222       8
 
BsmI GAATG_Cn'

Cuts at:      0    1521    2804    3963 
   Size:       1521    1283    1159

  Fragments arranged by size: 

                1521    1283    1159
 
Bsp1286I G_dGCh'C

Cuts at:      0       8    2357    3292    3516    3741    3963 
   Size:          8    2349     935     224     225     222

  Fragments arranged by size: 

                2349     935     225     224     222       8
 
BspEI T'CCGG_A

Cuts at:      0    2654    3963 
   Size:       2654    1309
 
BspHI T'CATG_A

Cuts at:      0    3621    3963 
   Size:       3621     342
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1360    2021    3963 
   Size:       1360     661    1942

  Fragments arranged by size: 

                1942    1360     661
 
BsrFI r'CCGG_y

Cuts at:      0      27    2419    3963 
   Size:         27    2392    1544

  Fragments arranged by size: 

                2392    1544      27
 
BsrGI T'GTAC_A

Cuts at:      0     324     729    3699    3963 
   Size:        324     405    2970     264

  Fragments arranged by size: 

                2970     405     324     264
 
BstEII G'GTnAC_C

Cuts at:      0    1808    3963 
   Size:       1808    2155
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2808    3963 
   Size:       2808    1155
 
BstYI r'GATC_y

Cuts at:      0     177     376    3282    3963 
   Size:        177     199    2906     681

  Fragments arranged by size: 

                2906     681     199     177
 
Bsu36I CC'TnA_GG

Cuts at:      0    1241    3963 
   Size:       1241    2722
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      92    3963 
   Size:         92    3871
 
BtsI GCAGTG_nn'

Cuts at:      0    2357    3208    3813    3914    3963 
   Size:       2357     851     605     101      49

  Fragments arranged by size: 

                2357     851     605     101      49
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1208    1243    3963 
   Size:       1208      35    2720

  Fragments arranged by size: 

                2720    1208      35
 
DraI TTT'AAA

Cuts at:      0     551    1451    2537    3963 
   Size:        551     900    1086    1426

  Fragments arranged by size: 

                1426    1086     900     551
 
DraIII CAC_nnn'GTG

Cuts at:      0    2022    3963 
   Size:       2022    1941
 
EaeI y'GGCC_r

Cuts at:      0      25    1865    2471    3142    3197    3277    3963 
   Size:         25    1840     606     671      55      80     686

  Fragments arranged by size: 

                1840     686     671     606      80      55      25
 
EarI CTCTTCn'nnn_

Cuts at:      0     665    2065    2125    3575    3963 
   Size:        665    1400      60    1450     388

  Fragments arranged by size: 

                1450    1400     665     388      60
 
EcoICRI GAG'CTC

Cuts at:      0    3739    3963 
   Size:       3739     224
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     202    1082    1151    1406    1445    1701    2341    2348 
   Size:        202     880      69     255      39     256     640       7

Cuts at:   2348    2957    2967    3347    3782    3803    3963 
   Size:        609      10     380     435      21     160

  Fragments arranged by size: 

                 880     640     609     435     380     256     255     202
                 160      69      39      21      10       7
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1240    3963 
   Size:       1240    2723
 
EcoO109I rG'GnC_Cy

Cuts at:      0     936    3289    3963 
   Size:        936    2353     674

  Fragments arranged by size: 

                2353     936     674
 
EcoRI G'AATT_C

Cuts at:      0    2667    3401    3835    3963 
   Size:       2667     734     434     128

  Fragments arranged by size: 

                2667     734     434     128
 
EcoRV GAT'ATC

Cuts at:      0    3844    3963 
   Size:       3844     119
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1266    1878    3454    3963 
   Size:       1266     612    1576     509

  Fragments arranged by size: 

                1576    1266     612     509
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     193     225    2777    2809    3963 
   Size:        193      32    2552      32    1154

  Fragments arranged by size: 

                2552    1154     193      32      32
 
HincII GTy'rAC

Cuts at:      0     669    1922    2496    3963 
   Size:        669    1253     574    1467

  Fragments arranged by size: 

                1467    1253     669     574
 
HindIII A'AGCT_T

Cuts at:      0     512    3963 
   Size:        512    3451
 
MfeI C'AATT_G

Cuts at:      0    1796    3963 
   Size:       1796    2167
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     338    2181    2357    3963 
   Size:        338    1843     176    1606

  Fragments arranged by size: 

                1843    1606     338     176
 
MscI TGG'CCA

Cuts at:      0    1867    3144    3199    3963 
   Size:       1867    1277      55     764

  Fragments arranged by size: 

                1867    1277     764      55
 
MslI CAynn'nnrTG

Cuts at:      0     533    3362    3527    3775    3963 
   Size:        533    2829     165     248     188

  Fragments arranged by size: 

                2829     533     248     188     165
 
MspA1I CmG'CkG

Cuts at:      0      95    1166    2389    2486    3963 
   Size:         95    1071    1223      97    1477

  Fragments arranged by size: 

                1477    1223    1071      97      95
 
NaeI GCC'GGC

Cuts at:      0    2421    3963 
   Size:       2421    1542
 
NgoMIV G'CCGG_C

Cuts at:      0    2419    3963 
   Size:       2419    1544
 
NspI r_CATG'y

Cuts at:      0     752    1327    3808    3963 
   Size:        752     575    2481     155

  Fragments arranged by size: 

                2481     752     575     155
 
PciI A'CATG_T

Cuts at:      0     748    3963 
   Size:        748    3215
 
PfoI T'CCnGG_A

Cuts at:      0     133    1525    2178    3963 
   Size:        133    1392     653    1785

  Fragments arranged by size: 

                1785    1392     653     133
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2195    2227    3963 
   Size:       2195      32    1736

  Fragments arranged by size: 

                2195    1736      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     936    3963 
   Size:        936    3027
 
PspOMI G'GGCC_C

Cuts at:      0    3288    3963 
   Size:       3288     675
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     308     340    3963 
   Size:        308      32    3623

  Fragments arranged by size: 

                3623     308      32
 
PstI C_TGCA'G

Cuts at:      0     918    1310    3963 
   Size:        918     392    2653

  Fragments arranged by size: 

                2653     918     392
 
PvuII CAG'CTG

Cuts at:      0      95    1166    2389    3963 
   Size:         95    1071    1223    1574

  Fragments arranged by size: 

                1574    1223    1071      95
 
SacI G_AGCT'C

Cuts at:      0    3741    3963 
   Size:       3741     222
 
SapI GCTCTTCn'nnn_

Cuts at:      0    3575    3963 
   Size:       3575     388
 
ScaI AGT'ACT

Cuts at:      0     954    3963 
   Size:        954    3009
 
SfcI C'TryA_G

Cuts at:      0     240     914    1306    2675    3963 
   Size:        240     674     392    1369    1288

  Fragments arranged by size: 

                1369    1288     674     392     240
 
SmlI C'TyrA_G

Cuts at:      0    1001    2698    2780    3133    3439    3963 
   Size:       1001    1697      82     353     306     524

  Fragments arranged by size: 

                1697    1001     524     353     306      82
 
StuI AGG'CCT

Cuts at:      0     844    1585    2778    3963 
   Size:        844     741    1193    1185

  Fragments arranged by size: 

                1193    1185     844     741
 
StyI C'CwwG_G

Cuts at:      0     984    3963 
   Size:        984    2979
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2010    2383    3659    3963 
   Size:       2010     373    1276     304

  Fragments arranged by size: 

                2010    1276     373     304
 
TatI w'GTAC_w

Cuts at:      0     324     729     910     952    1168    1856    3218    3699 
   Size:        324     405     181      42     216     688    1362     481

Cuts at:   3699    3963 
   Size:        264

  Fragments arranged by size: 

                1362     688     481     405     324     264     216     181
                  42
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2036    2068    2699    2731    3963 
   Size:       2036      32     631      32    1232

  Fragments arranged by size: 

                2036    1232     631      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     611    1827    3515    3963 
   Size:        611    1216    1688     448

  Fragments arranged by size: 

                1688    1216     611     448
 
XmnI GAAnn'nnTTC

Cuts at:      0    3086    3963 
   Size:       3086     877
 

 Enzymes that do cut:

     AarI     AccI     AclI     AcuI    AflII   AflIII     AlfI     AloI
    AlwNI     ApaI     ApoI     AseI     AvaI    BamHI    BanII     BbsI
     BclI    BglII Bme1580I    BmgBI     BmrI     BpmI    BpuEI     BsaI
    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrFI    BsrGI   BstEII    BstXI    BstYI
   Bsu36I    BtgZI     BtsI    CspCI     DraI   DraIII     EaeI     EarI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI    Hin4I
   HincII  HindIII     MfeI     MmeI     MscI     MslI   MspA1I     NaeI
   NgoMIV     NspI     PciI     PfoI     PpiI    PpuMI   PspOMI     PsrI
     PstI    PvuII     SacI     SapI     ScaI     SfcI     SmlI     StuI
     StyI    TaqII     TatI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AfeI     AgeI     AhdI     AleI    ApaLI     AscI
    AsiSI    AvrII     BaeI     BanI     BbeI    BbvCI     BcgI    BciVI
    BfrBI     BglI     BlpI     BmtI     BplI   Bpu10I    BsaAI    BsaBI
    BsaHI    BsiEI    BsiWI    BsmBI    BsrBI    BsrDI   BssHII    BssSI
   BstAPI    BstBI  BstZ17I     BtgI     ClaI     DrdI     EagI     EciI
     FseI     FspI    FspAI    HaeII     HpaI     KasI     KpnI     MluI
     NarI     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PasI    PflMI     PmeI     PmlI    PshAI     PsiI    PspXI     PvuI
    RsrII    SacII     SalI    SanDI     SbfI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI  Tth111I     XbaI     XhoI     XmaI     ZraI
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