Restriction map for pF1KSDA0679
##################################
# NO CUT ENZYMES (  108 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AlwNI       Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AviII       
BbeI        BbrPI       BcgI        BfrI        BglI        
BglII       BsaAI       BsaHI       BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       BsmI        BspDI       
BspEI       BspHI       BspMI       BssHII      BstBI       
BstEII      BstPI       BstUI       CfoI        ClaI        
CpoI        DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoNI       EcoO65I     EcoT22I     
FseI        FspI        HaeII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HpaI        
KasI        KpnI        KspI        MluI        MroI        
MspA1I      MvnI        NarI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        PvuII       RcaI        
RsrII       SacII       SalI        SapI        SbfI        
SfiI        SfoI        SfuI        SgrAI       SnaBI       
SphI        Sse8387I    SspI        StuI        SwaI        
Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccI         2412  GTATAC               GT^[AC][GT]AC
AflIII       1916  ACATGT               A^C[GA][CT]GT
AocI         2164  CCTAAGG              CC^T[ACGT]AGG
ApaI          879  GGGCCC               GGGCC^C
AvaI         2618  CCCGGG               C^[CT]CG[GA]G
AvrII         615  CCTAGG               C^CTAGG
BalI         2567  TGGCCA               TGG^CCA
BamHI        2390  GGATCC               G^GATCC
BanI          105  GGCACC               G^G[CT][GA]CC
BbvCI        2283  CCTCAGC              CCTCAGC(-5/-2)
BciVI         451  GTATCC               GTATCC(6/5)
BclI          858  TGATCA               T^GATCA
BlnI          615  CCTAGG               C^CTAGG
BsaBI         853  GATTATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI          31  GAGGAG               GAGGAG(10/8)
BsoBI        2618  CCCGGG               C^[CT]CG[GA]G
BspLU11I     1916  ACATGT               A^CATGT
BsrDI        1569  CATTGC               GCAATG(2/0)
BsrFI          93  GCCGGC               [GA]^CCGG[CT]
Bst1107I     2412  GTATAC               GTA^TAC
BstAPI       1608  GCAGTTTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I      2412  GTATAC               GTA^TAC
Bsu36I       2164  CCTAAGG              CC^T[ACGT]AGG
Cfr10I         93  GCCGGC               [GA]^CCGG[CT]
DraI         1266  TTTAAA               TTT^AAA
DraII         750  AGGTCCC              [GA]G^G[ACGT]CC[CT]
DraIII       1208  CACACAGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco81I       2164  CCTAAGG              CC^T[ACGT]AGG
EcoO109I      750  AGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoRI        2657  GAATTC               G^AATTC
FbaI          858  TGATCA               T^GATCA
HindIII       718  AAGCTT               A^AGCTT
MamI          853  GATTATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        2567  TGGCCA               TGG^CCA
MscI         2567  TGGCCA               TGG^CCA
NaeI           93  GCCGGC               GCC^GGC
NcoI         1391  CCATGG               C^CATGG
NdeI         2325  CATATG               CA^TATG
NgoMIV         93  GCCGGC               G^CCGGC
NheI         1143  GCTAGC               G^CTAGC
NspI         1916  ACATGT               [GA]CATG^[CT]
PflMI        2080  CCAATTGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         750  AGGTCCC              [GA]G^G[AT]CC[CT]
PstI          158  CTGCAG               CTGCA^G
SmaI         2618  CCCGGG               CCC^GGG
SmlI         1329  CTTGAG               C^T[CT][GA]AG
SpeI         1857  ACTAGT               A^CTAGT
TfiI         1522  GAATC                G^A[AT]TC
Van91I       2080  CCAATTGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI        2618  CCCGGG               C^CCGGG
XmaI         2618  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

Asp700I       715  GAAAAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2707  GAAGGATTTC          
BlpI           48  GCTCAGC              GC^T[ACGT]AGC
              346  GCTGAGC             
Bpu1102I       48  GCTCAGC              GC^T[ACGT]AGC
              346  GCTGAGC             
BsaI         2021  GAGACC               GGTCTC(1/5)
             2640  GAGACC              
BsrBI          34  GAGCGG               CCGCTC(-3/-3)
              140  GAGCGG              
BssSI         377  CACGAG               CACGAG(-5/-1)
             1826  CACGAG              
BstXI         823  CCAGTAATCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1391  CCATGGACATGG        
BstYI         895  AGATCC               [GA]^GATC[CT]
             2390  GGATCC              
CelII          48  GCTCAGC              GC^T[ACGT]AGC
              346  GCTGAGC             
DsaI          913  CCACGG               C^C[GA][CT]GG
             1391  CCATGG              
EaeI          149  TGGCCG               [CT]^GGCC[GA]
             2567  TGGCCA              
EcoRV         424  GATATC               GAT^ATC
             1936  GATATC              
MflI          895  AGATCC               [GA]^GATC[CT]
             2390  GGATCC              
SacI           46  GAGCTC               GAGCT^C
              818  GAGCTC              
ScaI         1051  AGTACT               AGT^ACT
             2487  AGTACT              
SexAI         476  ACCAGGT              A^CC[AT]GGT
              988  ACCTGGT             
SfaNI          79  GCATC                GCATC(5/9)
             2176  GCATC               
TaqI          791  TCGA                 T^CGA
             1755  TCGA                
TthHB8I       791  TCGA                 T^CGA
             1755  TCGA                
XcmI         1391  CCATGGACATGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2570  CCAAAAGCATGATGG     
XhoII         895  AGATCC               [GA]^GATC[CT]
             2390  GGATCC              
XmnI          715  GAAAAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2707  GAAGGATTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   77 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AspHI       AvaII       BanII       BbsI        
BbvI        BcnI        BfaI        BmyI        BpmI        
BpuAI       BsaJI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    Bsp1407I    
BsrGI       BsrI        BssKI       BstF5I      BstNI       
Cac8I       Cfr13I      DdeI        DpnII       EarI        
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MfeI        MnlI        MseI        
MslI        MspI        MunI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PleI        
RsaI        Sau3AI      Sau96I      ScrFI       SfcI        
SspBI       StyI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       
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