Restriction map for pF1KSDA0679
 (Linear) (Six-base) MAPSORT of: KIAA0679.seq  Check: 8747  from: 1  to: 2748



KIAA0679.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:28  ..

AccI GT'mk_AC

Cuts at:      0    2413    2748 
   Size:       2413     335
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1985    2623    2748 
   Size:       1985     638     125

  Fragments arranged by size: 

                1985     638     125
 
AflIII A'CryG_T

Cuts at:      0    1916    2748 
   Size:       1916     832
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1697    2748 
   Size:       1697    1051
 
ApaI G_GGCC'C

Cuts at:      0     883    2748 
   Size:        883    1865
 
ApoI r'AATT_y

Cuts at:      0     773     949    1741    2381    2657    2665    2748 
   Size:        773     176     792     640     276       8      83

  Fragments arranged by size: 

                 792     773     640     276     176      83       8
 
AvaI C'yCGr_G

Cuts at:      0    2618    2748 
   Size:       2618     130
 
AvrII C'CTAG_G

Cuts at:      0     615    2748 
   Size:        615    2133
 
BamHI G'GATC_C

Cuts at:      0    2390    2748 
   Size:       2390     358
 
BanI G'GyrC_C

Cuts at:      0     105    2748 
   Size:        105    2643
 
BanII G_rGCy'C

Cuts at:      0      50     171     822     883    1307    2748 
   Size:         50     121     651      61     424    1441

  Fragments arranged by size: 

                1441     651     424     121      61      50
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     122     216    2588    2748 
   Size:        122      94    2372     160

  Fragments arranged by size: 

                2372     160     122      94
 
BbvCI CC'TCA_GC

Cuts at:      0    2284    2748 
   Size:       2284     464
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     462    2748 
   Size:        462    2286
 
BclI T'GATC_A

Cuts at:      0     858    2748 
   Size:        858    1890
 
BlpI GC'TnA_GC

Cuts at:      0      49     347    2748 
   Size:         49     298    2401

  Fragments arranged by size: 

                2401     298      49
 
Bme1580I G_kGCm'C

Cuts at:      0     883    2347    2748 
   Size:        883    1464     401

  Fragments arranged by size: 

                1464     883     401
 
BmtI G_CTAG'C

Cuts at:      0    1147    2748 
   Size:       1147    1601
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      69    2748 
   Size:         69    2679
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     106     238     806    1684    2748 
   Size:        106     132     568     878    1064

  Fragments arranged by size: 

                1064     878     568     132     106
 
Bpu10I CC'TnA_GC

Cuts at:      0    2284    2748 
   Size:       2284     464
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1350    2748 
   Size:       1350    1398
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2015    2634    2748 
   Size:       2015     619     114

  Fragments arranged by size: 

                2015     619     114
 
BsaBI GATnn'nnATC

Cuts at:      0     857    2748 
   Size:        857    1891
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     131     161    1906    1936    2748 
   Size:        131      30    1745      30     812

  Fragments arranged by size: 

                1745     812     131      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      46    2748 
   Size:         46    2702
 
BseYI C'CCAG_C

Cuts at:      0     133    2748 
   Size:        133    2615
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      25     359     516     563    2748 
   Size:         25     334     157      47    2185

  Fragments arranged by size: 

                2185     334     157      47      25
 
BsiHKAI G_wGCw'C

Cuts at:      0      50     822    1728    2748 
   Size:         50     772     906    1020

  Fragments arranged by size: 

                1020     906     772      50
 
Bsp1286I G_dGCh'C

Cuts at:      0      50     171     822     883    1307    1728    2347    2748 
   Size:         50     121     651      61     424     421     619     401

  Fragments arranged by size: 

                 651     619     424     421     401     121      61      50
            
 
BsrBI CCG'CTC

Cuts at:      0      36     142    2748 
   Size:         36     106    2606

  Fragments arranged by size: 

                2606     106      36
 
BsrDI GCAATG_nn'

Cuts at:      0    1568    2748 
   Size:       1568    1180
 
BsrFI r'CCGG_y

Cuts at:      0      93    2748 
   Size:         93    2655
 
BsrGI T'GTAC_A

Cuts at:      0    1640    1971    2183    2748 
   Size:       1640     331     212     565

  Fragments arranged by size: 

                1640     565     331     212
 
BssSI C'ACGA_G

Cuts at:      0     377    1826    2748 
   Size:        377    1449     922

  Fragments arranged by size: 

                1449     922     377
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1614    2748 
   Size:       1614    1134
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     830    1398    2748 
   Size:        830     568    1350

  Fragments arranged by size: 

                1350     830     568
 
BstYI r'GATC_y

Cuts at:      0     895    2390    2748 
   Size:        895    1495     358

  Fragments arranged by size: 

                1495     895     358
 
BstZ17I GTA'TAC

Cuts at:      0    2414    2748 
   Size:       2414     334
 
Bsu36I CC'TnA_GG

Cuts at:      0    2165    2748 
   Size:       2165     583
 
BtgI C'CryG_G

Cuts at:      0     913    1391    2748 
   Size:        913     478    1357

  Fragments arranged by size: 

                1357     913     478
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      65    2748 
   Size:         65    2683
 
BtsI GCAGTG_nn'

Cuts at:      0    1095    1643    2748 
   Size:       1095     548    1105

  Fragments arranged by size: 

                1105    1095     548
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    2705    2740    2748 
   Size:       2705      35       8

  Fragments arranged by size: 

                2705      35       8
 
DraI TTT'AAA

Cuts at:      0    1268    2748 
   Size:       1268    1480
 
DraIII CAC_nnn'GTG

Cuts at:      0    1213    2748 
   Size:       1213    1535
 
EaeI y'GGCC_r

Cuts at:      0     149    2567    2748 
   Size:        149    2418     181

  Fragments arranged by size: 

                2418     181     149
 
EarI CTCTTCn'nnn_

Cuts at:      0     643     788     859    1838    2748 
   Size:        643     145      71     979     910

  Fragments arranged by size: 

                 979     910     643     145      71
 
EcoICRI GAG'CTC

Cuts at:      0      48     820    2748 
   Size:         48     772    1928

  Fragments arranged by size: 

                1928     772      48
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     106     238     806    1684    1985    2623    2748 
   Size:        106     132     568     878     301     638     125

  Fragments arranged by size: 

                 878     638     568     301     132     125     106
 
EcoO109I rG'GnC_Cy

Cuts at:      0     751    2748 
   Size:        751    1997
 
EcoRI G'AATT_C

Cuts at:      0    2657    2748 
   Size:       2657      91
 
EcoRV GAT'ATC

Cuts at:      0     426    1938    2748 
   Size:        426    1512     810

  Fragments arranged by size: 

                1512     810     426
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     410     442    1906    1938    2161    2193    2748 
   Size:        410      32    1464      32     223      32     555

  Fragments arranged by size: 

                1464     555     410     223      32      32      32
 
HindIII A'AGCT_T

Cuts at:      0     718    2748 
   Size:        718    2030
 
MfeI C'AATT_G

Cuts at:      0    1782    1887    2081    2748 
   Size:       1782     105     194     667

  Fragments arranged by size: 

                1782     667     194     105
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0       8      80    2719    2748 
   Size:          8      72    2639      29

  Fragments arranged by size: 

                2639      72      29       8
 
MscI TGG'CCA

Cuts at:      0    2569    2748 
   Size:       2569     179
 
MslI CAynn'nnrTG

Cuts at:      0     848    1396    1402    1433    2748 
   Size:        848     548       6      31    1315

  Fragments arranged by size: 

                1315     848     548      31       6
 
NaeI GCC'GGC

Cuts at:      0      95    2748 
   Size:         95    2653
 
NcoI C'CATG_G

Cuts at:      0    1391    2748 
   Size:       1391    1357
 
NdeI CA'TA_TG

Cuts at:      0    2326    2748 
   Size:       2326     422
 
NgoMIV G'CCGG_C

Cuts at:      0      93    2748 
   Size:         93    2655
 
NheI G'CTAG_C

Cuts at:      0    1143    2748 
   Size:       1143    1605
 
NspI r_CATG'y

Cuts at:      0    1920    2748 
   Size:       1920     828
 
PasI CC'CwG_GG

Cuts at:      0    2033    2748 
   Size:       2033     715
 
PciI A'CATG_T

Cuts at:      0    1916    2748 
   Size:       1916     832
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2086    2748 
   Size:       2086     662
 
PfoI T'CCnGG_A

Cuts at:      0    2386    2748 
   Size:       2386     362
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     379     411    2748 
   Size:        379      32    2337

  Fragments arranged by size: 

                2337     379      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     751    2748 
   Size:        751    1997
 
PspOMI G'GGCC_C

Cuts at:      0     879    2748 
   Size:        879    1869
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    2471    2503    2748 
   Size:       2471      32     245

  Fragments arranged by size: 

                2471     245      32
 
PstI C_TGCA'G

Cuts at:      0     162    2748 
   Size:        162    2586
 
SacI G_AGCT'C

Cuts at:      0      50     822    2748 
   Size:         50     772    1926

  Fragments arranged by size: 

                1926     772      50
 
ScaI AGT'ACT

Cuts at:      0    1053    2489    2748 
   Size:       1053    1436     259

  Fragments arranged by size: 

                1436    1053     259
 
SexAI A'CCwGG_T

Cuts at:      0     476     988    2748 
   Size:        476     512    1760

  Fragments arranged by size: 

                1760     512     476
 
SfcI C'TryA_G

Cuts at:      0     158    1119    2348    2748 
   Size:        158     961    1229     400

  Fragments arranged by size: 

                1229     961     400     158
 
SmaI CCC'GGG

Cuts at:      0    2620    2748 
   Size:       2620     128
 
SmlI C'TyrA_G

Cuts at:      0    1329    2748 
   Size:       1329    1419
 
SpeI A'CTAG_T

Cuts at:      0    1857    2748 
   Size:       1857     891
 
StyI C'CwwG_G

Cuts at:      0     214     443     615    1355    1391    2339    2748 
   Size:        214     229     172     740      36     948     409

  Fragments arranged by size: 

                 948     740     409     229     214     172      36
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     482     700    2748 
   Size:        482     218    2048

  Fragments arranged by size: 

                2048     482     218
 
TatI w'GTAC_w

Cuts at:      0    1051    1113    1640    1919    1971    2183    2487    2748 
   Size:       1051      62     527     279      52     212     304     261

  Fragments arranged by size: 

                1051     527     304     279     261     212      62      52
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     131     163     696     722     728     754    2748 
   Size:        131      32     533      26       6      26    1994

  Fragments arranged by size: 

                1994     533     131      32      26      26       6
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1398    2577    2748 
   Size:       1398    1179     171

  Fragments arranged by size: 

                1398    1179     171
 
XmaI C'CCGG_G

Cuts at:      0    2618    2748 
   Size:       2618     130
 
XmnI GAAnn'nnTTC

Cuts at:      0     719    2711    2748 
   Size:        719    1992      37

  Fragments arranged by size: 

                1992     719      37
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AlfI     ApaI     ApoI     AvaI    AvrII
    BamHI     BanI    BanII     BbsI    BbvCI    BciVI     BclI     BlpI
 Bme1580I     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaBI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BsrBI    BsrDI
    BsrFI    BsrGI    BssSI   BstAPI    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI    CspCI     DraI   DraIII     EaeI     EarI
  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV    Hin4I  HindIII     MfeI
     MmeI     MscI     MslI     NaeI     NcoI     NdeI   NgoMIV     NheI
     NspI     PasI     PciI    PflMI     PfoI     PpiI    PpuMI   PspOMI
     PsrI     PstI     SacI     ScaI    SexAI     SfcI     SmaI     SmlI
     SpeI     StyI    TaqII     TatI     TstI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AloI    AlwNI    ApaLI     AscI     AseI    AsiSI     BaeI
     BbeI     BcgI    BfrBI     BglI    BglII    BmgBI     BmrI    BsaAI
    BsaHI    BsaWI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI   BssHII    BstBI   BstEII     ClaI     DrdI     EagI     EciI
    EcoNI     FalI     FseI     FspI    FspAI    HaeII   HincII     HpaI
     KasI     KpnI     MluI   MspA1I     NarI     NotI     NruI     NsiI
     PacI     PmeI     PmlI    PshAI     PsiI    PspXI     PvuI    PvuII
    RsrII    SacII     SalI    SanDI     SapI     SbfI     SfiI     SfoI
     SgfI    SgrAI    SnaBI     SphI     SrfI     SspI     StuI     SwaI
  Tth111I     XbaI     XhoI     ZraI
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