Restriction map for pF1KSDA0438
 (Linear) (Six-base) MAPSORT of: KIAA0438.seq  Check: 1524  from: 1  to: 2124



KIAA0438.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:28  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2046    2124 
   Size:       2046      78
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     869    1444    1454    1709    2124 
   Size:        869     575      10     255     415

  Fragments arranged by size: 

                 869     575     415     255      10
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1699    1731    2124 
   Size:       1699      32     393

  Fragments arranged by size: 

                1699     393      32
 
ApoI r'AATT_y

Cuts at:      0     222     302     764    1496    1564    2124 
   Size:        222      80     462     732      68     560

  Fragments arranged by size: 

                 732     560     462     222      80      68
 
BamHI G'GATC_C

Cuts at:      0    1128    1707    2124 
   Size:       1128     579     417

  Fragments arranged by size: 

                1128     579     417
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     120     767    1637    1837    2124 
   Size:        120     647     870     200     287

  Fragments arranged by size: 

                 870     647     287     200     120
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     381    2124 
   Size:        381    1743
 
BlpI GC'TnA_GC

Cuts at:      0    1746    2124 
   Size:       1746     378
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     263    2124 
   Size:        263    1861
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     794    1632    2124 
   Size:        794     838     492

  Fragments arranged by size: 

                 838     794     492
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1602    1627    2124 
   Size:       1602      25     497

  Fragments arranged by size: 

                1602     497      25
 
Bpu10I CC'TnA_GC

Cuts at:      0     165    1406    2124 
   Size:        165    1241     718

  Fragments arranged by size: 

                1241     718     165
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     764    1125    1341    2124 
   Size:        764     361     216     783

  Fragments arranged by size: 

                 783     764     361     216
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1846    2124 
   Size:       1846     278
 
BseYI C'CCAG_C

Cuts at:      0    1370    2124 
   Size:       1370     754
 
BsmI GAATG_Cn'

Cuts at:      0    1294    2124 
   Size:       1294     830
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1563    2046    2124 
   Size:       1563     483      78

  Fragments arranged by size: 

                1563     483      78
 
BsrDI GCAATG_nn'

Cuts at:      0      38    2124 
   Size:         38    2086
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      46     243    2124 
   Size:         46     197    1881

  Fragments arranged by size: 

                1881     197      46
 
BstYI r'GATC_y

Cuts at:      0    1128    1707    2124 
   Size:       1128     579     417

  Fragments arranged by size: 

                1128     579     417
 
Bsu36I CC'TnA_GG

Cuts at:      0     228     393    2124 
   Size:        228     165    1731

  Fragments arranged by size: 

                1731     228     165
 
BtgI C'CryG_G

Cuts at:      0     915    2124 
   Size:        915    1209
 
BtsI GCAGTG_nn'

Cuts at:      0    1299    1431    1542    1799    1874    1920    2124 
   Size:       1299     132     111     257      75      46     204

  Fragments arranged by size: 

                1299     257     204     132     111      75      46
 
ClaI AT'CG_AT

Cuts at:      0    1326    2124 
   Size:       1326     798
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     959     994    2124 
   Size:        959      35    1130

  Fragments arranged by size: 

                1130     959      35
 
DraI TTT'AAA

Cuts at:      0     135    2124 
   Size:        135    1989
 
EaeI y'GGCC_r

Cuts at:      0    1808    2124 
   Size:       1808     316
 
EarI CTCTTCn'nnn_

Cuts at:      0     686    1463    1481    2124 
   Size:        686     777      18     643

  Fragments arranged by size: 

                 777     686     643      18
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     869    1444    1454    1602    1627    1709    2124 
   Size:        869     575      10     148      25      82     415

  Fragments arranged by size: 

                 869     575     415     148      82      25      10
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     137    2124 
   Size:        137    1987
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      28      60    1339    1371    2124 
   Size:         28      32    1279      32     753

  Fragments arranged by size: 

                1279     753      32      32      28
 
HindIII A'AGCT_T

Cuts at:      0     162    2124 
   Size:        162    1962
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     176     590    1568    2124 
   Size:        176     414     978     556

  Fragments arranged by size: 

                 978     556     414     176
 
MscI TGG'CCA

Cuts at:      0    1810    2124 
   Size:       1810     314
 
MslI CAynn'nnrTG

Cuts at:      0     122     371    2124 
   Size:        122     249    1753

  Fragments arranged by size: 

                1753     249     122
 
MspA1I CmG'CkG

Cuts at:      0    1204    2124 
   Size:       1204     920
 
NcoI C'CATG_G

Cuts at:      0     915    2124 
   Size:        915    1209
 
NdeI CA'TA_TG

Cuts at:      0    1701    2124 
   Size:       1701     423
 
NspI r_CATG'y

Cuts at:      0     121    2012    2124 
   Size:        121    1891     112

  Fragments arranged by size: 

                1891     121     112
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1758    2124 
   Size:       1758     366
 
PfoI T'CCnGG_A

Cuts at:      0     441    2124 
   Size:        441    1683
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1394    1426    2124 
   Size:       1394      32     698

  Fragments arranged by size: 

                1394     698      32
 
PstI C_TGCA'G

Cuts at:      0     555    2061    2124 
   Size:        555    1506      63

  Fragments arranged by size: 

                1506     555      63
 
SfcI C'TryA_G

Cuts at:      0     551    2057    2124 
   Size:        551    1506      67

  Fragments arranged by size: 

                1506     551      67
 
SmlI C'TyrA_G

Cuts at:      0     779    1140    1356    2124 
   Size:        779     361     216     768

  Fragments arranged by size: 

                 779     768     361     216
 
StyI C'CwwG_G

Cuts at:      0     915    1481    1501    2124 
   Size:        915     566      20     623

  Fragments arranged by size: 

                 915     623     566      20
 
TatI w'GTAC_w

Cuts at:      0       8     363     403    1511    2124 
   Size:          8     355      40    1108     613

  Fragments arranged by size: 

                1108     613     355      40       8
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1227    1259    2124 
   Size:       1227      32     865

  Fragments arranged by size: 

                1227     865      32
 

 Enzymes that do cut:

     AarI     AcuI     AloI     ApoI    BamHI     BbsI    BciVI     BlpI
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BseYI     BsmI
    BspMI    BsrDI    BstXI    BstYI   Bsu36I     BtgI     BtsI     ClaI
    CspCI     DraI     EaeI     EarI  Eco57MI     FalI    Hin4I  HindIII
     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NspI    PflMI
     PfoI     PsrI     PstI     SfcI     SmlI     StyI     TatI     TstI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI     BanI    BanII     BbeI    BbvCI
     BcgI     BclI    BfrBI     BglI    BglII Bme1580I    BmgBI     BmtI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI
  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I    BtgZI
   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FseI     FspI    FspAI    HaeII   HincII     HpaI     KasI
     KpnI     MfeI     MluI     NaeI     NarI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI
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