################################## # NO CUT ENZYMES ( 122 enzymes) # ################################## Acc65I AccII AccIII AclI AflII AflIII AhdI Alw44I ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaII AviII AvrII BamHI BbeI BbrPI BbsI BcgI BciVI BclI BfrI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssHII BstAPI BstBI BstEII BstPI BstUI CelII ClaI CpoI DraI EagI Eam1105I EclXI Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI MvnI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AatII 1513 GACGTC GACGT^C AccI 22 GTATAC GT^[AC][GT]AC AcsI 1075 GAATTT [GA]^AATT[CT] AgeI 77 ACCGGT A^CCGGT AocI 931 CCTCAGG CC^T[ACGT]AGG Aor51HI 134 AGCGCT AGC^GCT ApoI 1075 GAATTT [GA]^AATT[CT] AspHI 1688 GAGCTC G[AT]GC[AT]^C AvaI 1805 CCCGAG C^[CT]CG[GA]G BalI 648 TGGCCA TGG^CCA BanII 1688 GAGCTC G[GA]GC[CT]^C BbvCI 1567 GCTGAGG CCTCAGC(-5/-2) BcnI 207 CCGGG CC^[GC]GG BglI 8 GCCTGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1409 AGATCT A^GATCT BsaWI 77 ACCGGT [AT]^CCGG[AT] BseRI 1049 GAGGAG GAGGAG(10/8) BsiEI 732 CGACCG CG[GA][CT]^CG BsiHKAI 1688 GAGCTC G[AT]GC[AT]^C BsmAI 1787 GTCTC GTCTC(1/5) BsmFI 492 GGGAC GGGAC(10/14) BsmI 1575 GAATGC GAATGC(1/-1) BsoBI 1805 CCCGAG C^[CT]CG[GA]G BspMI 466 ACCTGC ACCTGC(4/8) BsrFI 77 ACCGGT [GA]^CCGG[CT] BssSI 1442 CTCGTG CACGAG(-5/-1) Bst1107I 22 GTATAC GTA^TAC BstXI 897 CCATGCTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 22 GTATAC GTA^TAC Bsu36I 931 CCTCAGG CC^T[ACGT]AGG CfoI 135 GCGC GCG^C Cfr10I 77 ACCGGT [GA]^CCGG[CT] DraIII 551 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 244 GACGGGTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 134 AGCGCT AGC^GCT Eco81I 931 CCTCAGG CC^T[ACGT]AGG HaeII 134 AGCGCT [GA]GCGC^[CT] HgaI 660 GACGC GACGC(5/10) HhaI 135 GCGC GCG^C HinP1I 135 GCGC G^CGC HincII 1474 GTCAAC GT[CT]^[GA]AC HindII 1474 GTCAAC GT[CT]^[GA]AC HindIII 752 AAGCTT A^AGCTT MluNI 648 TGGCCA TGG^CCA MscI 648 TGGCCA TGG^CCA NciI 207 CCGGG CC^[GC]GG NdeI 973 CATATG CA^TATG NspI 382 GCATGC [GA]CATG^[CT] PinAI 77 ACCGGT A^CCGGT PstI 1518 CTGCAG CTGCA^G SacI 1688 GAGCTC GAGCT^C SapI 967 GCTCTTC GCTCTTC(1/4) SphI 382 GCATGC GCATG^C StuI 981 AGGCCT AGG^CCT TfiI 1422 GATTC G^A[AT]TC Tsp45I 1540 GTCAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcyI 660 GACGCC G[GA]^CG[CT]C 1513 GACGTC AlwNI 934 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG 1286 CAGATGCTG BsaHI 660 GACGCC G[GA]^CG[CT]C 1513 GACGTC BsiYI 368 CCCCCCTGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 369 CCCCCTGCGGG BslI 368 CCCCCCTGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 369 CCCCCTGCGGG BstNI 9 CCTGG CC^[AT]GG 1797 CCTGG DraII 5 AGGGCCT [GA]G^G[ACGT]CC[CT] 1208 AGGCCCC EarI 968 CTCTTC CTCTTC(1/4) 1861 GAAGAG EcoO109I 5 AGGGCCT [GA]G^G[ACGT]CC[CT] 1208 AGGCCCC EcoRII 9 CCTGG ^CC[AT]GG 1797 CCTGG EcoT14I 327 CCATGG C^C[AT][AT]GG 651 CCATGG FauI 161 CCCGC CCCGC(4/6) 375 GCGGG Hin1I 660 GACGCC G[GA]^CG[CT]C 1513 GACGTC Ksp632I 968 CTCTTC CTCTTC(1/4) 1861 GAAGAG MspA1I 284 CAGCTG C[AC]G^C[GT]G 581 CAGCTG MvaI 9 CCTGG CC^[AT]GG 1797 CCTGG NcoI 327 CCATGG C^CATGG 651 CCATGG PvuII 284 CAGCTG CAG^CTG 581 CAGCTG SfcI 635 CTATAG C^T[GA][CT]AG 1518 CTGCAG SmlI 1166 CTCAAG C^T[CT][GA]AG 1789 CTCAAG SspI 1018 AATATT AAT^ATT 1279 AATATT StyI 327 CCATGG C^C[AT][AT]GG 651 CCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 58 enzymes) # ######################################### AciI AfaI AluI AlwI BanI BbvI BfaI BmyI BpmI BsaJI BsgI Bsp1286I BsrDI BsrI BssKI BstF5I BstYI Cac8I Cfr13I DdeI DpnII DsaI EaeI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI TaqI Tru9I TseI Tsp509I TspRI TthHB8I XhoII