Restriction map for pF1KB7785
 (Linear) (Six-base) MAPSORT of: KIBB7785.seq  Check: 3854  from: 1  to: 1371



KIBB7785.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:24  ..

AccI GT'mk_AC

Cuts at:      0     513    1371 
   Size:        513     858
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52    1164    1248    1371 
   Size:         52    1112      84     123

  Fragments arranged by size: 

                1112     123      84      52
 
ApoI r'AATT_y

Cuts at:      0     317     794    1046    1332    1371 
   Size:        317     477     252     286      39

  Fragments arranged by size: 

                 477     317     286     252      39
 
BanII G_rGCy'C

Cuts at:      0    1276    1371 
   Size:       1276      95
 
BclI T'GATC_A

Cuts at:      0     305    1371 
   Size:        305    1066
 
BfrBI ATG'CAT

Cuts at:      0     568     908    1108    1371 
   Size:        568     340     200     263

  Fragments arranged by size: 

                 568     340     263     200
 
BglII A'GATC_T

Cuts at:      0     219    1371 
   Size:        219    1152
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     142     291     728    1371 
   Size:        142     149     437     643

  Fragments arranged by size: 

                 643     437     149     142
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1081    1371 
   Size:       1081     290
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     572     908    1076    1160    1244    1290    1371 
   Size:        572     336     168      84      84      46      81

  Fragments arranged by size: 

                 572     336     168      84      84      81      46
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     132    1371 
   Size:        132    1239
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      67    1371 
   Size:         67    1304
 
BseYI C'CCAG_C

Cuts at:      0     195    1371 
   Size:        195    1176
 
BsiHKAI G_wGCw'C

Cuts at:      0    1276    1371 
   Size:       1276      95
 
BsmI GAATG_Cn'

Cuts at:      0     910    1371 
   Size:        910     461
 
Bsp1286I G_dGCh'C

Cuts at:      0    1276    1371 
   Size:       1276      95
 
BsrGI T'GTAC_A

Cuts at:      0      89    1371 
   Size:         89    1282
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     496    1371 
   Size:        496     875
 
BstYI r'GATC_y

Cuts at:      0     219    1371 
   Size:        219    1152
 
BtsI GCAGTG_nn'

Cuts at:      0     855    1371 
   Size:        855     516
 
DraI TTT'AAA

Cuts at:      0     351    1371 
   Size:        351    1020
 
EaeI y'GGCC_r

Cuts at:      0      32     117    1371 
   Size:         32      85    1254

  Fragments arranged by size: 

                1254      85      32
 
EarI CTCTTCn'nnn_

Cuts at:      0    1123    1371 
   Size:       1123     248
 
EcoICRI GAG'CTC

Cuts at:      0    1274    1371 
   Size:       1274      97
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     572     908    1076    1160    1164    1244    1248 
   Size:         52     520     336     168      84       4      80       4

Cuts at:   1248    1290    1371 
   Size:         42      81

  Fragments arranged by size: 

                 520     336     168      84      81      80      52      42
                   4       4
 
EcoO109I rG'GnC_Cy

Cuts at:      0     331     476    1371 
   Size:        331     145     895

  Fragments arranged by size: 

                 895     331     145
 
EcoRI G'AATT_C

Cuts at:      0     317     794    1046    1371 
   Size:        317     477     252     325

  Fragments arranged by size: 

                 477     325     317     252
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     277     309    1290    1322    1371 
   Size:        277      32     981      32      49

  Fragments arranged by size: 

                 981     277      49      32      32
 
HincII GTy'rAC

Cuts at:      0     514    1371 
   Size:        514     857
 
HindIII A'AGCT_T

Cuts at:      0    1007    1371 
   Size:       1007     364
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      86     797    1371 
   Size:         86     711     574

  Fragments arranged by size: 

                 711     574      86
 
MscI TGG'CCA

Cuts at:      0      34     119    1371 
   Size:         34      85    1252

  Fragments arranged by size: 

                1252      85      34
 
MslI CAynn'nnrTG

Cuts at:      0     494     567    1371 
   Size:        494      73     804

  Fragments arranged by size: 

                 804     494      73
 
MspA1I CmG'CkG

Cuts at:      0    1268    1371 
   Size:       1268     103
 
NdeI CA'TA_TG

Cuts at:      0     452     564    1371 
   Size:        452     112     807

  Fragments arranged by size: 

                 807     452     112
 
NsiI A_TGCA'T

Cuts at:      0     570     910    1110    1371 
   Size:        570     340     200     261

  Fragments arranged by size: 

                 570     340     261     200
 
NspI r_CATG'y

Cuts at:      0     572    1108    1371 
   Size:        572     536     263

  Fragments arranged by size: 

                 572     536     263
 
PvuII CAG'CTG

Cuts at:      0    1268    1371 
   Size:       1268     103
 
SacI G_AGCT'C

Cuts at:      0    1276    1371 
   Size:       1276      95
 
SalI G'TCGA_C

Cuts at:      0     512    1371 
   Size:        512     859
 
ScaI AGT'ACT

Cuts at:      0     465    1371 
   Size:        465     906
 
SphI G_CATG'C

Cuts at:      0    1108    1371 
   Size:       1108     263
 
StuI AGG'CCT

Cuts at:      0     757    1371 
   Size:        757     614
 
TatI w'GTAC_w

Cuts at:      0      89     463     739    1371 
   Size:         89     374     276     632

  Fragments arranged by size: 

                 632     374     276      89
 
XmnI GAAnn'nnTTC

Cuts at:      0     317     863    1009    1371 
   Size:        317     546     146     362

  Fragments arranged by size: 

                 546     362     317     146
 

 Enzymes that do cut:

     AccI     AcuI     ApoI    BanII     BclI    BfrBI    BglII     BmrI
     BplI     BpmI     BsaI    BseRI    BseYI  BsiHKAI     BsmI Bsp1286I
    BsrGI    BstXI    BstYI     BtsI     DraI     EaeI     EarI  EcoICRI
  Eco57MI EcoO109I    EcoRI    Hin4I   HincII  HindIII     MmeI     MscI
     MslI   MspA1I     NdeI     NsiI     NspI    PvuII     SacI     SalI
     ScaI     SphI     StuI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
     BbsI    BbvCI     BcgI    BciVI     BglI     BlpI Bme1580I    BmgBI
     BmtI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I
   Bsu36I     BtgI    BtgZI     ClaI    CspCI   DraIII     DrdI     EagI
     EciI    EcoNI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
     HpaI     KasI     KpnI     MfeI     MluI     NaeI     NarI     NcoI
   NgoMIV     NheI     NotI     NruI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII    SacII    SanDI     SapI
     SbfI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SrfI     SspI     StyI     SwaI    TaqII
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI
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