Restriction map for pF1KB9409
##################################
# NO CUT ENZYMES (  131 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AvaI        AvrII       BanI        BbeI        
BbrPI       BbvCI       BcgI        BciVI       BclI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaWI       BseAI       BsiWI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspLU11I    BsrBI       
BsrFI       BsrGI       BssHII      Bst1107I    BstBI       
BstEII      BstPI       BstXI       BstZ17I     CelII       
Cfr10I      ClaI        DraI        EagI        Eam1105I    
EclXI       Eco52I      EcoNI       EcoO65I     EcoRI       
EcoT14I     EcoT22I     FbaI        FseI        Hin1I       
HincII      HindII      HpaI        KasI        KpnI        
MaeI        MamI        MfeI        MluI        MroI        
MunI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
TfiI        XbaI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AocI          397  CCTCAGG              CC^T[ACGT]AGG
Aor51HI       104  AGCGCT               AGC^GCT
BamHI         802  GGATCC               G^GATCC
BbsI          226  GTCTTC               GAAGAC(2/6)
BcnI           27  CCCGG                CC^[GC]GG
BpuAI         226  GTCTTC               GAAGAC(2/6)
BsaI          340  GAGACC               GGTCTC(1/5)
BsgI          139  GTGCAG               GTGCAG(16/14)
BsiEI          69  CGACCG               CG[GA][CT]^CG
BsmBI          65  GAGACG               CGTCTC(1/5)
BspHI         468  TCATGA               T^CATGA
BsrDI         552  CATTGC               GCAATG(2/0)
BssSI          16  CACGAG               CACGAG(-5/-1)
BstYI         802  GGATCC               [GA]^GATC[CT]
Bsu36I        397  CCTCAGG              CC^T[ACGT]AGG
CpoI           29  CGGTCCG              CG^G[AT]CCG
DraII         269  GGGTCCC              [GA]G^G[ACGT]CC[CT]
DraIII         16  CACGAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          356  CCGCGG               C^C[GA][CT]GG
EarI          220  GAAGAG               CTCTTC(1/4)
Eco47III      104  AGCGCT               AGC^GCT
Eco81I        397  CCTCAGG              CC^T[ACGT]AGG
EcoO109I      269  GGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoRV         856  GATATC               GAT^ATC
HaeII         104  AGCGCT               [GA]GCGC^[CT]
HapII          28  CCGG                 C^CGG
HgaI           74  GCGTC                GACGC(5/10)
HindIII       731  AAGCTT               A^AGCTT
HinfI         276  GACTC                G^A[ACGT]TC
HpaII          28  CCGG                 C^CGG
Ksp632I       220  GAAGAG               CTCTTC(1/4)
KspI          356  CCGCGG               CCGC^GG
MflI          802  GGATCC               [GA]^GATC[CT]
MseI          575  TTAA                 T^TAA
MspI           28  CCGG                 C^CGG
NciI           27  CCCGG                CC^[GC]GG
PflMI          15  CCACGAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          276  GACTC                GAGTC(4/5)
PpuMI         269  GGGTCCC              [GA]G^G[AT]CC[CT]
PvuII         455  CAGCTG               CAG^CTG
RcaI          468  TCATGA               T^CATGA
RsrII          29  CGGTCCG              CG^G[AT]CCG
SacI          660  GAGCTC               GAGCT^C
SacII         356  CCGCGG               CCGC^GG
SphI          537  GCATGC               GCATG^C
Tru9I         575  TTAA                 T^TAA
Tsp509I       244  AATT                 ^AATT
TspRI         118  CAGTG                CA[GC]TG[ACGT][ACGT]^
Van91I         15  CCACGAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         802  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AccII          73  CGCG                 CG^CG
              357  CGCG                
AfaI          588  GTAC                 GT^AC
              780  GTAC                
AlwNI          82  CAGAACCTG            CAG[ACGT][ACGT][ACGT]^CTG
              400  CAGGAGCTG           
AspI           25  GACCCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
               70  GACCGCGTC           
AviII         164  TGCGCA               TGC^GCA
              491  TGCGCA              
BalI          176  TGGCCA               TGG^CCA
              236  TGGCCA              
BanII         637  GGGCTC               G[GA]GC[CT]^C
              660  GAGCTC              
BsaJI         300  CCTGGG               C^C[ACGT][ACGT]GG
              356  CCGCGG              
BspMI          56  ACCTGC               ACCTGC(4/8)
               86  ACCTGC              
BstAPI         81  GCAGAACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              483  GCACATCTTGC         
BstUI          73  CGCG                 CG^CG
              357  CGCG                
DrdI           67  GACGACCGCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              276  GACTCCGAAGTC        
EaeI          176  TGGCCA               [CT]^GGCC[GA]
              236  TGGCCA              
FspI          164  TGCGCA               TGC^GCA
              491  TGCGCA              
HphI           92  TCACC                GGTGA(8/7)
              704  TCACC               
MaeIII        460  GTCAC                ^GT[ACGT]AC
              797  GTGAC               
MluNI         176  TGGCCA               TGG^CCA
              236  TGGCCA              
MscI          176  TGGCCA               TGG^CCA
              236  TGGCCA              
MslI          180  CACCTGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              711  CACAGACGTG          
MvnI           73  CGCG                 CG^CG
              357  CGCG                
PflFI          25  GACCCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
               70  GACCGCGTC           
PstI           79  CTGCAG               CTGCA^G
              772  CTGCAG              
RsaI          588  GTAC                 GT^AC
              780  GTAC                
SfaNI         186  GATGC                GCATC(5/9)
              676  GATGC               
Tsp45I        460  GTCAC                ^GT[GC]AC
              797  GTGAC               
Tth111I        25  GACCCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
               70  GACCGCGTC           


#########################################
# 3 OR MORE CUT ENZYMES (   51 enzymes) #
#########################################

AciI        AluI        AlwI        AspHI       AvaII       
BbvI        BmyI        BpmI        BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       EcoRII      
FauI        Fnu4HI      FokI        HaeIII      HhaI        
HinP1I      ItaI        MaeII       MboI        MboII       
MnlI        MspA1I      MvaI        MwoI        NdeII       
NlaIII      NlaIV       NspI        Sau3AI      Sau96I      
ScrFI       SfcI        TaqI        TseI        TthHB8I     
XcmI        
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