Restriction map for pF1KB9359
 (Linear) (Six-base) MAPSORT of: KIBB9359.seq  Check: 4147  from: 1  to: 876



KIBB9359.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:28  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     725     876 
   Size:        725     151
 
AfeI AGC'GCT

Cuts at:      0     109     214     876 
   Size:        109     105     662

  Fragments arranged by size: 

                 662     109     105
 
AleI CACnn'nnGTG

Cuts at:      0     706     876 
   Size:        706     170
 
AlwNI CAG_nnn'CTG

Cuts at:      0      90     303     876 
   Size:         90     213     573

  Fragments arranged by size: 

                 573     213      90
 
ApaI G_GGCC'C

Cuts at:      0     833     876 
   Size:        833      43
 
AvaI C'yCGr_G

Cuts at:      0      38     809     876 
   Size:         38     771      67

  Fragments arranged by size: 

                 771      67      38
 
AvrII C'CTAG_G

Cuts at:      0     432     876 
   Size:        432     444
 
BanI G'GyrC_C

Cuts at:      0      22     646     876 
   Size:         22     624     230

  Fragments arranged by size: 

                 624     230      22
 
BanII G_rGCy'C

Cuts at:      0     674     817     833     876 
   Size:        674     143      16      43

  Fragments arranged by size: 

                 674     143      43      16
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     222     876 
   Size:        222     654
 
Bme1580I G_kGCm'C

Cuts at:      0     833     876 
   Size:        833      43
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     426     876 
   Size:        426     450
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     121     214     771     801     876 
   Size:        121      93     557      30      75

  Fragments arranged by size: 

                 557     121      93      75      30
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     427     876 
   Size:        427     449
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     337     876 
   Size:        337     539
 
BsaAI yAC'GTr

Cuts at:      0     114     876 
   Size:        114     762
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      25     831     861     876 
   Size:         25     806      30      15

  Fragments arranged by size: 

                 806      30      25      15
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     118     220     235     876 
   Size:        118     102      15     641

  Fragments arranged by size: 

                 641     118     102      15
 
BseYI C'CCAG_C

Cuts at:      0      63     792     832     876 
   Size:         63     729      40      44

  Fragments arranged by size: 

                 729      63      44      40
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     163     478     731     876 
   Size:        163     315     253     145

  Fragments arranged by size: 

                 315     253     163     145
 
BsiHKAI G_wGCw'C

Cuts at:      0     674     876 
   Size:        674     202
 
Bsp1286I G_dGCh'C

Cuts at:      0     674     817     833     876 
   Size:        674     143      16      43

  Fragments arranged by size: 

                 674     143      43      16
 
BsrBI CCG'CTC

Cuts at:      0     775     876 
   Size:        775     101
 
BsrFI r'CCGG_y

Cuts at:      0     497     876 
   Size:        497     379
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      90     876 
   Size:         90     786
 
Bsu36I CC'TnA_GG

Cuts at:      0     763     876 
   Size:        763     113
 
BtgI C'CryG_G

Cuts at:      0      55     611     876 
   Size:         55     556     265

  Fragments arranged by size: 

                 556     265      55
 
BtsI GCAGTG_nn'

Cuts at:      0     638     876 
   Size:        638     238
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      76     391     876 
   Size:         76     315     485

  Fragments arranged by size: 

                 485     315      76
 
EaeI y'GGCC_r

Cuts at:      0     239     876 
   Size:        239     637
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      85     876 
   Size:         85     791
 
EcoICRI GAG'CTC

Cuts at:      0     672     876 
   Size:        672     204
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     121     214     725     771     801     876 
   Size:        121      93     511      46      30      75

  Fragments arranged by size: 

                 511     121      93      75      46      30
 
EcoO109I rG'GnC_Cy

Cuts at:      0     450     829     876 
   Size:        450     379      47

  Fragments arranged by size: 

                 450     379      47
 
HaeII r_GCGC'y

Cuts at:      0     111     216     799     876 
   Size:        111     105     583      77

  Fragments arranged by size: 

                 583     111     105      77
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     831     863     876 
   Size:        831      32      13

  Fragments arranged by size: 

                 831      32      13
 
MscI TGG'CCA

Cuts at:      0     241     876 
   Size:        241     635
 
MslI CAynn'nnrTG

Cuts at:      0     706     876 
   Size:        706     170
 
MspA1I CmG'CkG

Cuts at:      0      57     147     876 
   Size:         57      90     729

  Fragments arranged by size: 

                 729      90      57
 
NspI r_CATG'y

Cuts at:      0     168     321     544     876 
   Size:        168     153     223     332

  Fragments arranged by size: 

                 332     223     168     153
 
PpuMI rG'GwC_Cy

Cuts at:      0     450     876 
   Size:        450     426
 
PspOMI G'GGCC_C

Cuts at:      0     829     876 
   Size:        829      47
 
PstI C_TGCA'G

Cuts at:      0      86     633     876 
   Size:         86     547     243

  Fragments arranged by size: 

                 547     243      86
 
SacI G_AGCT'C

Cuts at:      0     674     876 
   Size:        674     202
 
SacII CC_GC'GG

Cuts at:      0      58     876 
   Size:         58     818
 
SfcI C'TryA_G

Cuts at:      0      82     569     629     851     876 
   Size:         82     487      60     222      25

  Fragments arranged by size: 

                 487     222      82      60      25
 
SmaI CCC'GGG

Cuts at:      0      40     811     876 
   Size:         40     771      65

  Fragments arranged by size: 

                 771      65      40
 
SmlI C'TyrA_G

Cuts at:      0     442     876 
   Size:        442     434
 
SnaBI TAC'GTA

Cuts at:      0     114     876 
   Size:        114     762
 
SphI G_CATG'C

Cuts at:      0     321     544     876 
   Size:        321     223     332

  Fragments arranged by size: 

                 332     321     223
 
StuI AGG'CCT

Cuts at:      0     526     641     876 
   Size:        526     115     235

  Fragments arranged by size: 

                 526     235     115
 
StyI C'CwwG_G

Cuts at:      0     432     527     825     876 
   Size:        432      95     298      51

  Fragments arranged by size: 

                 432     298      95      51
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     295     533     634     876 
   Size:        295     238     101     242

  Fragments arranged by size: 

                 295     242     238     101
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      27     461     493     876 
   Size:         27     434      32     383

  Fragments arranged by size: 

                 434     383      32      27
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     249     876 
   Size:        249     627
 
XmaI C'CCGG_G

Cuts at:      0      38     809     876 
   Size:         38     771      67

  Fragments arranged by size: 

                 771      67      38
 

 Enzymes that do cut:

     AcuI     AfeI     AleI    AlwNI     ApaI     AvaI    AvrII     BanI
    BanII     BbsI Bme1580I     BmrI     BpmI    BpuEI     BsaI    BsaAI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BsrBI    BsrFI
   BstAPI   Bsu36I     BtgI     BtsI     DrdI     EaeI     EciI  EcoICRI
  Eco57MI EcoO109I    HaeII    Hin4I     MscI     MslI   MspA1I     NspI
    PpuMI   PspOMI     PstI     SacI    SacII     SfcI     SmaI     SmlI
    SnaBI     SphI     StuI     StyI    TaqII     TstI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI
     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI    BmgBI     BmtI     BplI   Bpu10I    BsaBI
    BsaHI    BsaWI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrDI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI
    BstYI  BstZ17I    BtgZI     ClaI    CspCI     DraI   DraIII     EagI
     EarI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII    RsrII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SpeI     SrfI     SspI     SwaI     TatI  Tth111I
     XbaI     XhoI     XmnI     ZraI
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