Restriction map for pF1KB9101
##################################
# NO CUT ENZYMES (  110 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AcyI        AflIII      AgeI        Aor51HI     ApaI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AviII       AvrII       BanI        BanII       BbeI        
BbrPI       BbvCI       BciVI       BclI        BcnI        
BglI        BglII       BlnI        BsaAI       BsaHI       
BsaWI       BseAI       BsiEI       BsiWI       BsmBI       
BspDI       BspEI       BspLU11I    BsrBI       BsrDI       
BsrFI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstZ17I     
Cfr10I      ClaI        CpoI        DrdI        DsaI        
EagI        EclXI       Eco47III    Eco52I      EcoO65I     
EcoT14I     FbaI        FseI        FspI        HaeII       
HapII       HgaI        Hin1I       HpaII       KasI        
KpnI        KspI        MluI        MroI        MspI        
MvnI        NaeI        NarI        NciI        NcoI        
NgoMIV      NotI        NruI        NspV        PacI        
PinAI       PmaCI       PmeI        PmlI        PshBI       
PvuI        RsrII       SacI        SacII       SalI        
SbfI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        Sse8387I    
StuI        StyI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AclI         1869  AACGTT               AA^CGTT
AflII        2196  CTTAAG               C^TTAAG
AspI         1184  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          839  CTCGAG               C^[CT]CG[GA]G
BamHI        1413  GGATCC               G^GATCC
BcgI          831  GCAGTATTCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI         2196  CTTAAG               C^TTAAG
BlpI         2747  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     2747  GCTCAGC              GC^T[ACGT]AGC
BseRI         653  CTCCTC               GAGGAG(10/8)
BsoBI         839  CTCGAG               C^[CT]CG[GA]G
Bsp1407I     2954  TGTACA               T^GTACA
BsrGI        2954  TGTACA               T^GTACA
CelII        2747  GCTCAGC              GC^T[ACGT]AGC
DraIII       1904  CACAATGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoNI        2080  CCTGAACCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV        1435  GATATC               GAT^ATC
EcoT22I        76  ATGCAT               ATGCA^T
FauI         2850  CCCGC                CCCGC(4/6)
HpaI         1962  GTTAAC               GTT^AAC
MfeI         1504  CAATTG               C^AATTG
MunI         1504  CAATTG               C^AATTG
NsiI           76  ATGCAT               ATGCA^T
PaeR7I        839  CTCGAG               C^TCGAG
PflFI        1184  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI        2506  GACAGGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     1869  AACGTT               AA^CGTT
SphI          230  GCATGC               GCATG^C
SspBI        2954  TGTACA               T^GTACA
SspI         1247  AATATT               AAT^ATT
SwaI         2724  ATTTAAAT             ATTT^AAAT
Tth111I      1184  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI          839  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AhdI          112  GACAAAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2126  GACGTACTGTC         
AocI         1555  CCTCAGG              CC^T[ACGT]AGG
             2422  CCTCAGG             
AspEI         112  GACAAAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2126  GACGTACTGTC         
BbsI         2395  GAAGAC               GAAGAC(2/6)
              264  GTCTTC              
BpmI         1646  CTCCAG               CTGGAG(16/14)
             2137  CTCCAG              
BpuAI        2395  GAAGAC               GAAGAC(2/6)
              264  GTCTTC              
BsaBI         598  GATACGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1255  GATAACAATC          
BsaI         1944  GAGACC               GGTCTC(1/5)
             1688  GGTCTC              
BsmI          279  GAATGC               GAATGC(1/-1)
             1913  GAATGC              
BspMI        2376  ACCTGC               ACCTGC(4/8)
             1078  GCAGGT              
BstF5I       1922  CATCC                GGATG(2/0)
              930  GGATG               
BstXI         969  CCATCTTAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1925  CCATGTCAGTGG        
BstYI         223  GGATCT               [GA]^GATC[CT]
             1413  GGATCC              
Bsu36I       1555  CCTCAGG              CC^T[ACGT]AGG
             2422  CCTCAGG             
CfoI         2372  GCGC                 GCG^C
             3413  GCGC                
Eam1105I      112  GACAAAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2126  GACGTACTGTC         
Eco81I       1555  CCTCAGG              CC^T[ACGT]AGG
             2422  CCTCAGG             
EcoRI        1464  GAATTC               G^AATTC
             1731  GAATTC              
FokI         1922  CATCC                GGATG(9/13)
              930  GGATG               
HhaI         2372  GCGC                 GCG^C
             3413  GCGC                
HinP1I       2372  GCGC                 G^CGC
             3413  GCGC                
MamI          598  GATACGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1255  GATAACAATC          
MflI          223  GGATCT               [GA]^GATC[CT]
             1413  GGATCC              
MspA1I       1957  CAGCTG               C[AC]G^C[GT]G
             2812  CAGCTG              
NdeI         1742  CATATG               CA^TATG
             3382  CATATG              
NheI          345  GCTAGC               G^CTAGC
             1012  GCTAGC              
NspI          230  GCATGC               [GA]CATG^[CT]
             3209  ACATGC              
PvuII        1957  CAGCTG               CAG^CTG
             2812  CAGCTG              
XbaI         2538  TCTAGA               T^CTAGA
             3067  TCTAGA              
XcmI          924  CCAGAAGGATGTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2972  CCAGCAGTTGCATGG     
XhoII         223  GGATCT               [GA]^GATC[CT]
             1413  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   84 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        Alw44I      
AlwI        AlwNI       ApaLI       ApoI        AspHI       
AvaII       BalI        BbvI        BfaI        BmyI        
BsaJI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BspHI       BsrI        
BssKI       BstNI       Cac8I       Cfr13I      DdeI        
DpnII       DraI        DraII       EaeI        EarI        
EcoO109I    EcoRII      Fnu4HI      HaeIII      HincII      
HindII      HindIII     HinfI       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MluNI       MnlI        MscI        MseI        
MslI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       PflMI       PleI        PpuMI       PstI        
RcaI        RsaI        SapI        Sau3AI      Sau96I      
ScaI        ScrFI       SfaNI       SfcI        SmlI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      
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