Restriction map for pF1KB7024
##################################
# NO CUT ENZYMES (  123 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      Aor51HI     ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvrII       BalI        BamHI       BbrPI       
BbvCI       BcgI        BciVI       BclI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BsaAI       
BsaBI       BsaI        BsaWI       BseAI       BsiWI       
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrDI       Bst1107I    BstBI       BstXI       BstZ17I     
CelII       ClaI        CpoI        DraI        DraII       
DrdI        Eam1105I    EarI        Eco47III    EcoO109I    
EcoRI       EcoT14I     EcoT22I     FbaI        FseI        
HincII      HindII      HindIII     HpaI        KpnI        
Ksp632I     MaeII       MamI        MfeI        MluI        
MluNI       MroI        MscI        MseI        MunI        
NcoI        NdeI        NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfuI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
StuI        StyI        SwaI        Tru9I       Tth111I     
XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AocI          871  CCTCAGG              CC^T[ACGT]AGG
ApaI          408  GGGCCC               GGGCC^C
AspHI         295  GTGCTC               G[AT]GC[AT]^C
AvaII           8  GGACC                G^G[AT]CC
AviII         438  TGCGCA               TGC^GCA
BbsI          807  GAAGAC               GAAGAC(2/6)
BglI          244  GCCGGCGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI         807  GAAGAC               GAAGAC(2/6)
BsiHKAI       295  GTGCTC               G[AT]GC[AT]^C
BsmBI          34  CGTCTC               CGTCTC(1/5)
BsmI          507  GCATTC               GAATGC(1/-1)
Bsp1407I      995  TGTACA               T^GTACA
BsrBI         313  GAGCGG               CCGCTC(-3/-3)
BsrGI         995  TGTACA               T^GTACA
BssHII        428  GCGCGC               G^CGCGC
BssSI        1147  CACGAG               CACGAG(-5/-1)
BstEII        211  GGTCACC              G^GT[ACGT]ACC
BstPI         211  GGTCACC              G^GT[ACGT]ACC
Bsu36I        871  CCTCAGG              CC^T[ACGT]AGG
Eco81I        871  CCTCAGG              CC^T[ACGT]AGG
EcoNI         183  CCTGGGCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       211  GGTCACC              G^GT[ACGT]ACC
EcoRV         853  GATATC               GAT^ATC
FspI          438  TGCGCA               TGC^GCA
MslI          776  CACACTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NlaIII        142  CATG                 CATG^
PflMI         336  CCAGGCCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SmlI          298  CTCAAG               C^T[CT][GA]AG
SspBI         995  TGTACA               T^GTACA
TaqI         1106  TCGA                 T^CGA
TthHB8I      1106  TCGA                 T^CGA
Van91I        336  CCAGGCCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AlwNI         677  CAGATGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1236  CAGCAGCTG           
BanII         408  GGGCCC               G[GA]GC[CT]^C
              841  GAGCCC              
BfaI          610  CTAG                 C^TAG
             1255  CTAG                
BsgI          928  GTGCAG               GTGCAG(16/14)
             1183  GTGCAG              
BsiEI         251  CGGCCG               CG[GA][CT]^CG
              387  CGGCCG              
BsmAI          35  GTCTC                GTCTC(1/5)
              615  GTCTC               
BsmFI         358  GGGAC                GGGAC(10/14)
              348  GTCCC               
BstYI         270  GGATCT               [GA]^GATC[CT]
              498  GGATCT              
EagI          251  CGGCCG               C^GGCCG
              387  CGGCCG              
EclXI         251  CGGCCG               C^GGCCG
              387  CGGCCG              
Eco52I        251  CGGCCG               C^GGCCG
              387  CGGCCG              
HphI          213  TCACC                GGTGA(8/7)
              588  TCACC               
KspI          236  CCGCGG               CCGC^GG
              351  CCGCGG              
MaeI          610  CTAG                 C^TAG
             1255  CTAG                
MflI          270  GGATCT               [GA]^GATC[CT]
              498  GGATCT              
NaeI          244  GCCGGC               GCC^GGC
              424  GCCGGC              
NgoMIV        244  GCCGGC               G^CCGGC
              424  GCCGGC              
SacII         236  CCGCGG               CCGC^GG
              351  CCGCGG              
SgrAI         243  CGCCGGCG             C[GA]^CCGG[CT]G
              423  CGCCGGCG            
SmaI          162  CCCGGG               CCC^GGG
              411  CCCGGG              
TfiI          541  GAATC                G^A[AT]TC
              742  GATTC               
Tsp45I        212  GTCAC                ^GT[GC]AC
              774  GTCAC               
Tsp509I       715  AATT                 ^AATT
              904  AATT                
XcmI          635  CCAGATACCCGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1055  CCAGGACGCTGTTGG     
XhoII         270  GGATCT               [GA]^GATC[CT]
              498  GGATCT              
XmaCI         162  CCCGGG               C^CCGGG
              411  CCCGGG              
XmaI          162  CCCGGG               C^CCGGG
              411  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   76 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AvaI        BanI        BbeI        BbvI        
BcnI        BmyI        BpmI        BsaHI       BsaJI       
BseRI       BsiYI       BslI        BsoBI       Bsp1286I    
BsrFI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstUI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraIII      DsaI        
EaeI        EcoRII      FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       ItaI        
KasI        MaeIII      MboI        MboII       MnlI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NarI        NciI        NdeII       NlaIV       PleI        
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SfoI        TseI        
TspRI       
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