Restriction map for pF1KB7024
 (Circular) (Six-base) MAPSORT of: pF1KB7024.seq  Check: 6002  from: 1  to: 4391



pF1KB7024  4391 bp

 With 182 enzymes: SgfI * 

                        February 20, 2009 16:23  ..

AccI GT'mk_AC

Cuts at:   1429    1429 
   Size:       4391
 
Acc65I G'GTAC_C

Cuts at:   1407    1407 
   Size:       4391
 
AclI AA'CG_TT

Cuts at:   4231    4231 
   Size:       4391
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    403     436    2216    2648    3001    3663     403 
   Size:         33    1780     432     353     662    1131

  Fragments arranged by size: 

                1780    1131     662     432     353      33
 
AflIII A'CryG_T

Cuts at:   1743    3543    3824    1743 
   Size:       1800     281    2310

  Fragments arranged by size: 

                2310    1800     281
 
AleI CACnn'nnGTG

Cuts at:    888     888 
   Size:       4391
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    319     319 
   Size:       4391
 
AlwNI CAG_nnn'CTG

Cuts at:    790    1349    1696    3134     790 
   Size:        559     347    1438    2047

  Fragments arranged by size: 

                2047    1438     559     347
 
ApaI G_GGCC'C

Cuts at:    520     520 
   Size:       4391
 
ApaLI G'TGCA_C

Cuts at:   3229    3229 
   Size:       4391
 
ApoI r'AATT_y

Cuts at:   1395    3744    4000    1395 
   Size:       2349     256    1786

  Fragments arranged by size: 

                2349    1786     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4391
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4391
 
AvaI C'yCGr_G

Cuts at:    270     519     670    1411     270 
   Size:        249     151     741    3250

  Fragments arranged by size: 

                3250     741     249     151
 
BamHI G'GATC_C

Cuts at:   1416    1416 
   Size:       4391
 
BanI G'GyrC_C

Cuts at:    310     349     367     525    1407    1821    2069    2104     310 
   Size:         39      18     158     882     414     248      35    2597

  Fragments arranged by size: 

                2597     882     414     248     158      39      35      18
            
 
BanII G_rGCy'C

Cuts at:    520     953    1405    2435     520 
   Size:        433     452    1030    2476

  Fragments arranged by size: 

                2476    1030     452     433
 
BbeI G_GCGC'C

Cuts at:    353     371     529    1825    2073     353 
   Size:         18     158    1296     248    2671

  Fragments arranged by size: 

                2671    1296     248     158      18
 
BbsI GAAGACnn'nnnn_

Cuts at:    922     922 
   Size:       4391
 
BciVI GTATCCnnnnn_n'

Cuts at:   2289    3345    4391    2289 
   Size:       1056    1046    2289

  Fragments arranged by size: 

                2289    1056    1046
 
BclI T'GATC_A

Cuts at:   1911    1911 
   Size:       4391
 
BglI GCCn_nnn'nGGC

Cuts at:    358     358 
   Size:       4391
 
BglII A'GATC_T

Cuts at:   1906    3943    1906 
   Size:       2037    2354
 
BlpI GC'TnA_GC

Cuts at:   1503    1503 
   Size:       4391
 
Bme1580I G_kGCm'C

Cuts at:    520    2016    2109    3233     520 
   Size:       1496      93    1124    1678

  Fragments arranged by size: 

                1678    1496    1124      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    915    1603    1684    2019    4163     915 
   Size:        688      81     335    2144    1143

  Fragments arranged by size: 

                2144    1143     688     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2758    2758 
   Size:       4391
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    168     427     451     726     886    1146     168 
   Size:        259      24     275     160     260    3413

  Fragments arranged by size: 

                3413     275     260     259     160      24
 
Bpu10I CC'TnA_GC

Cuts at:    700     700 
   Size:       4391
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    391    1567    2446    3160    3458     391 
   Size:       1176     879     714     298    1324

  Fragments arranged by size: 

                1324    1176     879     714     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   4095    4095 
   Size:       4391
 
BsaAI yAC'GTr

Cuts at:   1744    1744 
   Size:       4391
 
BsaBI GATnn'nnATC

Cuts at:      6    1905    3942       6 
   Size:       1899    2037     455

  Fragments arranged by size: 

                2037    1899     455
 
BsaHI Gr'CG_yC

Cuts at:    141     350     368     526    1822    2070    2772    4268     141 
   Size:        209      18     158    1296     248     702    1496     264

  Fragments arranged by size: 

                1496    1296     702     264     248     209     158      18
            
 
BsaWI w'CCGG_w

Cuts at:   1580    2101    2779    3190    3337    3611    1580 
   Size:        521     678     411     147     274    2360

  Fragments arranged by size: 

                2360     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    141     171    1144    1174     141 
   Size:         30     973      30    3358

  Fragments arranged by size: 

                3358     973      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    165     193     196     218     221     925     165 
   Size:         28       3      22       3     704    3631

  Fragments arranged by size: 

                3631     704      28      22       3       3
 
BseYI C'CCAG_C

Cuts at:    513    1313    1816    2574    3239    3647     513 
   Size:        800     503     758     665     408    1257

  Fragments arranged by size: 

                1257     800     758     665     503     408
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1057    1312    1057 
   Size:        255    4136
 
BsiEI CG_ry'CG

Cuts at:    104     362     498    1933    1979    3209     104 
   Size:        258     136    1435      46    1230    1286

  Fragments arranged by size: 

                1435    1286    1230     258     136      46
 
BsiHKAI G_wGCw'C

Cuts at:    407    1405    2183    2373    3233     407 
   Size:        998     778     190     860    1565

  Fragments arranged by size: 

                1565     998     860     778     190
 
BsmI GAATG_Cn'

Cuts at:    615     615 
   Size:       4391
 
BsmBI CGTCTCn'nnnn_

Cuts at:    148     148 
   Size:       4391
 
Bsp1286I G_dGCh'C

Cuts at:    407     520     953    1405    2016    2109    2183    2373    2435 
   Size:        113     433     452     611      93      74     190      62

Cuts at:   2435    3233     407 
   Size:        798    1565

  Fragments arranged by size: 

                1565     798     611     452     433     190     113      93
                  74      62
 
BspHI T'CATG_A

Cuts at:   2823    2823 
   Size:       4391
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1441    1655    1957    2338    1441 
   Size:        214     302     381    3494

  Fragments arranged by size: 

                3494     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     423    2683    2737    4221    4386      50 
   Size:        373    2260      54    1484     165      55

  Fragments arranged by size: 

                2260    1484     373     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2303    2303 
   Size:       4391
 
BsrFI r'CCGG_y

Cuts at:    256     352     532    2389    2570     256 
   Size:         96     180    1857     181    2077

  Fragments arranged by size: 

                2077    1857     181     180      96
 
BsrGI T'GTAC_A

Cuts at:   1103    1103 
   Size:       4391
 
BssHII G'CGCG_C

Cuts at:    536     536 
   Size:       4391
 
BssSI C'ACGA_G

Cuts at:   1255    2662    3370    1255 
   Size:       1407     708    2276

  Fragments arranged by size: 

                2276    1407     708
 
BstAPI GCAn_nnn'nTGC

Cuts at:    402     479    1044    1768     402 
   Size:         77     565     724    3025

  Fragments arranged by size: 

                3025     724     565      77
 
BstBI TT'CG_AA

Cuts at:   2752    2752 
   Size:       4391
 
BstEII G'GTnAC_C

Cuts at:    319     319 
   Size:       4391
 
BstYI r'GATC_y

Cuts at:      1     378     606    1416    1883    1906    2241    2487    2793 
   Size:        377     228     810     467      23     335     246     306

Cuts at:   2793    2805    2891    3943       1 
   Size:         12      86    1052     449

  Fragments arranged by size: 

                1052     810     467     449     377     335     306     246
                 228      86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    980     980 
   Size:       4391
 
BtgI C'CryG_G

Cuts at:    344     449     459    1273     344 
   Size:        105      10     814    3462

  Fragments arranged by size: 

                3462     814     105      10
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2341    2522    2685    2341 
   Size:        181     163    4047

  Fragments arranged by size: 

                4047     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1634    1634 
   Size:       4391
 
DraI TTT'AAA

Cuts at:   1390    1390 
   Size:       4391
 
DraIII CAC_nnn'GTG

Cuts at:    402     479    1185     402 
   Size:         77     706    3608

  Fragments arranged by size: 

                3608     706      77
 
DrdI GACnn_nn'nnGTC

Cuts at:   2097    3441    2097 
   Size:       1344    3047
 
EaeI y'GGCC_r

Cuts at:    359     495     601    1976    2150    2541    2568    3913     359 
   Size:        136     106    1375     174     391      27    1345     837

  Fragments arranged by size: 

                1375    1345     837     391     174     136     106      27
            
 
EagI C'GGCC_G

Cuts at:    359     495    1976     359 
   Size:        136    1481    2774

  Fragments arranged by size: 

                2774    1481     136
 
EarI CTCTTCn'nnn_

Cuts at:   2414    2624    2414 
   Size:        210    4181
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    391    1128    2599    3341    3487    4201     391 
   Size:        737    1471     742     146     714     581

  Fragments arranged by size: 

                1471     742     737     714     581     146
 
EcoICRI GAG'CTC

Cuts at:   1403    1403 
   Size:       4391
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    168     403     427     436     451     726     886    1146    2216 
   Size:        235      24       9      15     275     160     260    1070

Cuts at:   2216    2648    3001    3663     168 
   Size:        432     353     662     896

  Fragments arranged by size: 

                1070     896     662     432     353     275     260     235
                 160      24      15       9
 
EcoNI CCTnn'n_nnAGG

Cuts at:    295     295 
   Size:       4391
 
EcoO109I rG'GnC_Cy

Cuts at:   1530    1530 
   Size:       4391
 
EcoRI G'AATT_C

Cuts at:   1395    1395 
   Size:       4391
 
EcoRV GAT'ATC

Cuts at:    963     963 
   Size:       4391
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2410    2410 
   Size:       4391
 
FspI TGC'GCA

Cuts at:    548    2172     548 
   Size:       1624    2767
 
HaeII r_GCGC'y

Cuts at:    353     371     391     529    1825    2073    3303    4074     353 
   Size:         18      20     138    1296     248    1230     771     670

  Fragments arranged by size: 

                1296    1230     771     670     248     138      20      18
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    116     148    2233    2263    2265    2295     116 
   Size:         32    2085      30       2      30    2212

  Fragments arranged by size: 

                2212    2085      32      30      30       2
 
HincII GTy'rAC

Cuts at:   1430    1430 
   Size:       4391
 
KasI G'GCGC_C

Cuts at:    349     367     525    1821    2069     349 
   Size:         18     158    1296     248    2671

  Fragments arranged by size: 

                2671    1296     248     158      18
 
KpnI G_GTAC'C

Cuts at:   1411    1411 
   Size:       4391
 
MluI A'CGCG_T

Cuts at:   3824    3824 
   Size:       4391
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   3150    3334    3150 
   Size:        184    4207
 
MscI TGG'CCA

Cuts at:   2152    2152 
   Size:       4391
 
MslI CAynn'nnrTG

Cuts at:    888    2380    2507     888 
   Size:       1492     127    2772

  Fragments arranged by size: 

                2772    1492     127
 
MspA1I CmG'CkG

Cuts at:    346     429     461     805    1032    1074    1242    1349    1502 
   Size:         83      32     344     227      42     168     107     153

Cuts at:   1502    1816    2176    2960    3205     346 
   Size:        314     360     784     245    1532

  Fragments arranged by size: 

                1532     784     360     344     314     245     227     168
                 153     107      83      42      32
 
NaeI GCC'GGC

Cuts at:    354     534    2572     354 
   Size:        180    2038    2173

  Fragments arranged by size: 

                2173    2038     180
 
NarI GG'CG_CC

Cuts at:    350     368     526    1822    2070     350 
   Size:         18     158    1296     248    2671

  Fragments arranged by size: 

                2671    1296     248     158      18
 
NgoMIV G'CCGG_C

Cuts at:    352     532    2570     352 
   Size:        180    2038    2173

  Fragments arranged by size: 

                2173    2038     180
 
NspI r_CATG'y

Cuts at:   1444    1944    3547    1444 
   Size:        500    1603    2288

  Fragments arranged by size: 

                2288    1603     500
 
PasI CC'CwG_GG

Cuts at:    835     835 
   Size:       4391
 
PciI A'CATG_T

Cuts at:   3543    3543 
   Size:       4391
 
PflMI CCAn_nnn'nTGG

Cuts at:    450     450 
   Size:       4391
 
PfoI T'CCnGG_A

Cuts at:    944    1162     944 
   Size:        218    4173
 
PmeI GTTT'AAAC

Cuts at:   1390    1390 
   Size:       4391
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2837    2869    2837 
   Size:         32    4359
 
PspOMI G'GGCC_C

Cuts at:    516     516 
   Size:       4391
 
PstI C_TGCA'G

Cuts at:    511     777    1241    1438    2123     511 
   Size:        266     464     197     685    2779

  Fragments arranged by size: 

                2779     685     464     266     197
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4391
 
PvuII CAG'CTG

Cuts at:    429    1032    1074    1349    1816    2176     429 
   Size:        603      42     275     467     360    2644

  Fragments arranged by size: 

                2644     603     467     360     275      42
 
RsrII CG'GwC_CG

Cuts at:   2586    2586 
   Size:       4391
 
SacI G_AGCT'C

Cuts at:   1405    1405 
   Size:       4391
 
SacII CC_GC'GG

Cuts at:    347     462     347 
   Size:        115    4276
 
SalI G'TCGA_C

Cuts at:   1428    1428 
   Size:       4391
 
SapI GCTCTTCn'nnn_

Cuts at:   2414    2624    2414 
   Size:        210    4181
 
SbfI CC_TGCA'GG

Cuts at:   1438    1438 
   Size:       4391
 
ScaI AGT'ACT

Cuts at:   3908    3908 
   Size:       4391
 
SfcI C'TryA_G

Cuts at:     33     507     773    1237    1434    2119    3087    3278      33 
   Size:        474     266     464     197     685     968     191    1146

  Fragments arranged by size: 

                1146     968     685     474     464     266     197     191
            
 
SfoI GGC'GCC

Cuts at:    351     369     527    1823    2071     351 
   Size:         18     158    1296     248    2671

  Fragments arranged by size: 

                2671    1296     248     158      18
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4391
 
SgrAI Cr'CCGG_yG

Cuts at:    352     532     352 
   Size:        180    4211
 
SmaI CCC'GGG

Cuts at:    272     521    1413     272 
   Size:        249     892    3250

  Fragments arranged by size: 

                3250     892     249
 
SmlI C'TyrA_G

Cuts at:    406    1546    2461    3175    3437     406 
   Size:       1140     915     714     262    1360

  Fragments arranged by size: 

                1360    1140     915     714     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4391
 
SphI G_CATG'C

Cuts at:   1444    1944    1444 
   Size:        500    3891
 
SrfI GCCC'GGGC

Cuts at:    272     272 
   Size:       4391
 
StyI C'CwwG_G

Cuts at:   1525    1879    1525 
   Size:        354    4037
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    869    1089    1974    2107    2566    2775     869 
   Size:        220     885     133     459     209    2485

  Fragments arranged by size: 

                2485     885     459     220     209     133
 
TatI w'GTAC_w

Cuts at:   1103    3906    1103 
   Size:       2803    1588
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    141     173    1081    1113    3908    3940     141 
   Size:         32     908      32    2795      32     592

  Fragments arranged by size: 

                2795     908     592      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2188    2188 
   Size:       4391
 
XbaI T'CTAG_A

Cuts at:   1422    1422 
   Size:       4391
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    750    1170    1886     750 
   Size:        420     716    3255

  Fragments arranged by size: 

                3255     716     420
 
XmaI C'CCGG_G

Cuts at:    270     519    1411     270 
   Size:        249     892    3250

  Fragments arranged by size: 

                3250     892     249
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AleI     AlfI    AlwNI
     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI
   Bsu36I     BtgI    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV
     NspI     PasI     PciI    PflMI     PfoI     PmeI     PpiI   PspOMI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI     SapI
     SbfI     ScaI     SfcI     SfoI     SgfI    SgrAI     SmaI     SmlI
     SpeI     SphI     SrfI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AgeI     AhdI     AloI     AscI
    AvrII     BaeI    BbvCI     BcgI    BfrBI    BmgBI     BmtI    BsiWI
    BspEI    BstXI  BstZ17I     ClaI    CspCI     FseI    FspAI  HindIII
     HpaI     MfeI     NcoI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PmlI    PpuMI    PshAI     PsiI    PspXI     PsrI    SanDI
    SexAI     SfiI    SnaBI     SspI     StuI     SwaI     XhoI     XmnI
     ZraI
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