Restriction map for pF1KB7023
 (Linear) (Six-base) MAPSORT of: KIBB7023.seq  Check: 3214  from: 1  to: 3318



KIBB7023.orf

 With 182 enzymes: SgfI * 

                        July 23, 2007 10:03  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     877    1827    2904    3318 
   Size:        877     950    1077     414

  Fragments arranged by size: 

                1077     950     877     414
 
AatII G_ACGT'C

Cuts at:      0    2282    3318 
   Size:       2282    1036
 
AccI GT'mk_AC

Cuts at:      0     617    3318 
   Size:        617    2701
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1975    2400    3318 
   Size:       1975     425     918

  Fragments arranged by size: 

                1975     918     425
 
AfeI AGC'GCT

Cuts at:      0    1083    3318 
   Size:       1083    2235
 
AflIII A'CryG_T

Cuts at:      0    1112    2654    3318 
   Size:       1112    1542     664

  Fragments arranged by size: 

                1542    1112     664
 
AhdI GACnn_n'nnGTC

Cuts at:      0    2298    2658    3318 
   Size:       2298     360     660

  Fragments arranged by size: 

                2298     660     360
 
AleI CACnn'nnGTG

Cuts at:      0     527    3318 
   Size:        527    2791
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2173    2608    3318 
   Size:       2173     435     710

  Fragments arranged by size: 

                2173     710     435
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1283    1315    3318 
   Size:       1283      32    2003

  Fragments arranged by size: 

                2003    1283      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      96    1171    3318 
   Size:         96    1075    2147

  Fragments arranged by size: 

                2147    1075      96
 
ApaI G_GGCC'C

Cuts at:      0    1979    3109    3318 
   Size:       1979    1130     209

  Fragments arranged by size: 

                1979    1130     209
 
AvaI C'yCGr_G

Cuts at:      0     111     162     855    2543    2872    3088    3290    3318 
   Size:        111      51     693    1688     329     216     202      28

  Fragments arranged by size: 

                1688     693     329     216     202     111      51      28
            
 
BamHI G'GATC_C

Cuts at:      0    2669    3318 
   Size:       2669     649
 
BanI G'GyrC_C

Cuts at:      0     235    1569    2701    3278    3318 
   Size:        235    1334    1132     577      40

  Fragments arranged by size: 

                1334    1132     577     235      40
 
BanII G_rGCy'C

Cuts at:      0     206    1720    1979    2144    2171    3109    3197    3221 
   Size:        206    1514     259     165      27     938      88      24

Cuts at:   3221    3233    3318 
   Size:         12      85

  Fragments arranged by size: 

                1514     938     259     206     165      88      85      27
                  24      12
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     857    3318 
   Size:        857    2461
 
BbvCI CC'TCA_GC

Cuts at:      0    1208    3318 
   Size:       1208    2110
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     693     727    1483    1517    3318 
   Size:        693      34     756      34    1801

  Fragments arranged by size: 

                1801     756     693      34      34
 
BclI T'GATC_A

Cuts at:      0     519    2372    3318 
   Size:        519    1853     946

  Fragments arranged by size: 

                1853     946     519
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1615    2166    3318 
   Size:       1615     551    1152

  Fragments arranged by size: 

                1615    1152     551
 
BglII A'GATC_T

Cuts at:      0      83    2522    2684    2795    3318 
   Size:         83    2439     162     111     523

  Fragments arranged by size: 

                2439     523     162     111      83
 
BlpI GC'TnA_GC

Cuts at:      0     983    2392    3318 
   Size:        983    1409     926

  Fragments arranged by size: 

                1409     983     926
 
Bme1580I G_kGCm'C

Cuts at:      0     970    1979    2258    3109    3283    3318 
   Size:        970    1009     279     851     174      35

  Fragments arranged by size: 

                1009     970     851     279     174      35
 
BmgBI CAC'GTC

Cuts at:      0    2655    3318 
   Size:       2655     663
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     281     830    1455    3318 
   Size:        281     549     625    1863

  Fragments arranged by size: 

                1863     625     549     281
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1889    3318 
   Size:       1889    1429
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     123     228     343     610    1062    1279    1447    2369 
   Size:        123     105     115     267     452     217     168     922

Cuts at:   2369    3133    3211    3217    3318 
   Size:        764      78       6     101

  Fragments arranged by size: 

                 922     764     452     267     217     168     123     115
                 105     101      78       6
 
Bpu10I CC'TnA_GC

Cuts at:      0    1208    1864    2647    3318 
   Size:       1208     656     783     671

  Fragments arranged by size: 

                1208     783     671     656
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    3247    3318 
   Size:       3247      71
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     319    1114    1349    2054    2468    3318 
   Size:        319     795     235     705     414     850

  Fragments arranged by size: 

                 850     795     705     414     319     235
 
BsaAI yAC'GTr

Cuts at:      0     945    1375    1497    1743    1775    3318 
   Size:        945     430     122     246      32    1543

  Fragments arranged by size: 

                1543     945     430     246     122      32
 
BsaHI Gr'CG_yC

Cuts at:      0    2279    3318 
   Size:       2279    1039
 
BsaWI w'CCGG_w

Cuts at:      0     174    2613    3318 
   Size:        174    2439     705

  Fragments arranged by size: 

                2439     705     174
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     295     325     802     832    1111    1141    1447    1477 
   Size:        295      30     477      30     279      30     306      30

Cuts at:   1477    2051    2081    2998    3028    3318 
   Size:        574      30     917      30     290

  Fragments arranged by size: 

                 917     574     477     306     295     290     279      30
                  30      30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1429    1465    1468    2938    2949    3153    3318 
   Size:       1429      36       3    1470      11     204     165

  Fragments arranged by size: 

                1470    1429     204     165      36      11       3
 
BseYI C'CCAG_C

Cuts at:      0    1325    1395    1803    2131    2179    2771    2819    2855 
   Size:       1325      70     408     328      48     592      48      36

Cuts at:   2855    2946    3124    3221    3318 
   Size:         91     178      97      97

  Fragments arranged by size: 

                1325     592     408     328     178      97      97      91
                  70      48      48      36
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     463     682     811    1506    1995    2062    3055    3318 
   Size:        463     219     129     695     489      67     993     263

  Fragments arranged by size: 

                 993     695     489     463     263     219     129      67
            
 
BsiHKAI G_wGCw'C

Cuts at:      0     254    1480    1631    1720    2144    2498    3233    3318 
   Size:        254    1226     151      89     424     354     735      85

  Fragments arranged by size: 

                1226     735     424     354     254     151      89      85
            
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     651    2270    3318 
   Size:        651    1619    1048

  Fragments arranged by size: 

                1619    1048     651
 
Bsp1286I G_dGCh'C

Cuts at:      0     206     254     970    1480    1631    1720    1979    2144 
   Size:        206      48     716     510     151      89     259     165

Cuts at:   2144    2171    2258    2498    3109    3197    3221    3233    3283 
   Size:         27      87     240     611      88      24      12      50

Cuts at:   3283    3318 
   Size:         35

  Fragments arranged by size: 

                 716     611     510     259     240     206     165     151
                  89      88      87      50      48      35      27      24
                  12
 
BspEI T'CCGG_A

Cuts at:      0    2613    3318 
   Size:       2613     705
 
BspHI T'CATG_A

Cuts at:      0    1961    3318 
   Size:       1961    1357
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     166     877     999    1827    2084    2365    2904    3318 
   Size:        166     711     122     828     257     281     539     414

  Fragments arranged by size: 

                 828     711     539     414     281     257     166     122
            
 
BsrBI CCG'CTC

Cuts at:      0     336     818     938    3318 
   Size:        336     482     120    2380

  Fragments arranged by size: 

                2380     482     336     120
 
BsrDI GCAATG_nn'

Cuts at:      0     879    2157    3318 
   Size:        879    1278    1161

  Fragments arranged by size: 

                1278    1161     879
 
BsrFI r'CCGG_y

Cuts at:      0     777    3318 
   Size:        777    2541
 
BssSI C'ACGA_G

Cuts at:      0     481    2407    3318 
   Size:        481    1926     911

  Fragments arranged by size: 

                1926     911     481
 
BstEII G'GTnAC_C

Cuts at:      0    2758    3318 
   Size:       2758     560
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1395    2313    2439    3318 
   Size:       1395     918     126     879

  Fragments arranged by size: 

                1395     918     879     126
 
BstYI r'GATC_y

Cuts at:      0      83     363    1688    2522    2669    2684    2795    3318 
   Size:         83     280    1325     834     147      15     111     523

  Fragments arranged by size: 

                1325     834     523     280     147     111      83      15
            
 
BtgI C'CryG_G

Cuts at:      0     315     371     926    1786    1850    2985    3318 
   Size:        315      56     555     860      64    1135     333

  Fragments arranged by size: 

                1135     860     555     333     315      64      56
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      31    3318 
   Size:         31    3287
 
BtsI GCAGTG_nn'

Cuts at:      0     475     502     665    1003    1480    1932    3318 
   Size:        475      27     163     338     477     452    1386

  Fragments arranged by size: 

                1386     477     475     452     338     163      27
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     741     776    1562    1597    3318 
   Size:        741      35     786      35    1721

  Fragments arranged by size: 

                1721     786     741      35      35
 
EaeI y'GGCC_r

Cuts at:      0     218    1173    1311    1504    1725    1892    2429    3318 
   Size:        218     955     138     193     221     167     537     889

  Fragments arranged by size: 

                 955     889     537     221     218     193     167     138
            
 
EarI CTCTTCn'nnn_

Cuts at:      0    1406    2819    3318 
   Size:       1406    1413     499

  Fragments arranged by size: 

                1413    1406     499
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     664    3310    3318 
   Size:        664    2646       8

  Fragments arranged by size: 

                2646     664       8
 
EcoICRI GAG'CTC

Cuts at:      0    1718    2142    3231    3318 
   Size:       1718     424    1089      87

  Fragments arranged by size: 

                1718    1089     424      87
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     123     228     343     610    1062    1279    1447    1975 
   Size:        123     105     115     267     452     217     168     528

Cuts at:   1975    2369    2400    3133    3211    3217    3318 
   Size:        394      31     733      78       6     101

  Fragments arranged by size: 

                 733     528     452     394     267     217     168     123
                 115     105     101      78      31       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     958    3318 
   Size:        958    2360
 
EcoO109I rG'GnC_Cy

Cuts at:      0      93     860    1826    1976    2006    2023    2354    3105 
   Size:         93     767     966     150      30      17     331     751

Cuts at:   3105    3318 
   Size:        213

  Fragments arranged by size: 

                 966     767     751     331     213     150      93      30
                  17
 
FspI TGC'GCA

Cuts at:      0    1531    3318 
   Size:       1531    1787
 
HaeII r_GCGC'y

Cuts at:      0    1085    2124    3318 
   Size:       1085    1039    1194

  Fragments arranged by size: 

                1194    1085    1039
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       9      41    1799    1831    1950    1982    2012    2044 
   Size:          9      32    1758      32     119      32      30      32

Cuts at:   2044    2284    2316    2644    2676    2998    3030    3318 
   Size:        240      32     328      32     322      32     288

  Fragments arranged by size: 

                1758     328     322     288     240     119      32      32
                  32      32      32      32      32      30       9
 
HincII GTy'rAC

Cuts at:      0     729    1995    3156    3318 
   Size:        729    1266    1161     162

  Fragments arranged by size: 

                1266    1161     729     162
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1097    1928    2138    2330    2813    3318 
   Size:       1097     831     210     192     483     505

  Fragments arranged by size: 

                1097     831     505     483     210     192
 
MscI TGG'CCA

Cuts at:      0     220    1175    2431    3318 
   Size:        220     955    1256     887

  Fragments arranged by size: 

                1256     955     887     220
 
MslI CAynn'nnrTG

Cuts at:      0     355     527     697     902     925    1202    1567    1741 
   Size:        355     172     170     205      23     277     365     174

Cuts at:   1741    2224    3318 
   Size:        483    1094

  Fragments arranged by size: 

                1094     483     365     355     277     205     174     172
                 170      23
 
MspA1I CmG'CkG

Cuts at:      0     465    1304    1425    1758    1788    2859    3318 
   Size:        465     839     121     333      30    1071     459

  Fragments arranged by size: 

                1071     839     465     459     333     121      30
 
NcoI C'CATG_G

Cuts at:      0    2985    3318 
   Size:       2985     333
 
NspI r_CATG'y

Cuts at:      0    1323    3318 
   Size:       1323    1995
 
PasI CC'CwG_GG

Cuts at:      0    1068    3318 
   Size:       1068    2250
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2916    3318 
   Size:       2916     402
 
PmlI CAC'GTG

Cuts at:      0    1375    1497    1775    3318 
   Size:       1375     122     278    1543

  Fragments arranged by size: 

                1543    1375     278     122
 
PpuMI rG'GwC_Cy

Cuts at:      0     860    2023    2354    3318 
   Size:        860    1163     331     964

  Fragments arranged by size: 

                1163     964     860     331
 
PshAI GACnn'nnGTC

Cuts at:      0     670    3318 
   Size:        670    2648
 
PspOMI G'GGCC_C

Cuts at:      0    1975    3105    3318 
   Size:       1975    1130     213

  Fragments arranged by size: 

                1975    1130     213
 
PstI C_TGCA'G

Cuts at:      0    1010    1354    1493    2105    3318 
   Size:       1010     344     139     612    1213

  Fragments arranged by size: 

                1213    1010     612     344     139
 
PvuII CAG'CTG

Cuts at:      0     465    1425    1758    2859    3318 
   Size:        465     960     333    1101     459

  Fragments arranged by size: 

                1101     960     465     459     333
 
SacI G_AGCT'C

Cuts at:      0    1720    2144    3233    3318 
   Size:       1720     424    1089      85

  Fragments arranged by size: 

                1720    1089     424      85
 
SacII CC_GC'GG

Cuts at:      0    1789    3318 
   Size:       1789    1529
 
ScaI AGT'ACT

Cuts at:      0    2047    3318 
   Size:       2047    1271
 
SexAI A'CCwGG_T

Cuts at:      0    2063    2423    3318 
   Size:       2063     360     895

  Fragments arranged by size: 

                2063     895     360
 
SfcI C'TryA_G

Cuts at:      0     618    1006    1231    1350    1489    2101    2145    3318 
   Size:        618     388     225     119     139     612      44    1173

  Fragments arranged by size: 

                1173     618     612     388     225     139     119      44
            
 
SmaI CCC'GGG

Cuts at:      0     113    3318 
   Size:        113    3205
 
SmlI C'TyrA_G

Cuts at:      0    2543    2872    3226    3318 
   Size:       2543     329     354      92

  Fragments arranged by size: 

                2543     354     329      92
 
SphI G_CATG'C

Cuts at:      0    1323    3318 
   Size:       1323    1995
 
StuI AGG'CCT

Cuts at:      0    1024    3318 
   Size:       1024    2294
 
StyI C'CwwG_G

Cuts at:      0     221    1971    2693    2985    3318 
   Size:        221    1750     722     292     333

  Fragments arranged by size: 

                1750     722     333     292     221
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     316     343    2686    2948    2960    3318 
   Size:        316      27    2343     262      12     358

  Fragments arranged by size: 

                2343     358     316     262      27      12
 
TatI w'GTAC_w

Cuts at:      0    1733    2045    3318 
   Size:       1733     312    1273

  Fragments arranged by size: 

                1733    1273     312
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     295     327     494     526     802     834    2051    2083 
   Size:        295      32     167      32     276      32    1217      32

Cuts at:   2083    3262    3294    3318 
   Size:       1179      32      24

  Fragments arranged by size: 

                1217    1179     295     276     167      32      32      32
                  32      32      24
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1282    1617    1887    2431    3318 
   Size:       1282     335     270     544     887

  Fragments arranged by size: 

                1282     887     544     335     270
 
XhoI C'TCGA_G

Cuts at:      0    2543    2872    3318 
   Size:       2543     329     446

  Fragments arranged by size: 

                2543     446     329
 
XmaI C'CCGG_G

Cuts at:      0     111    3318 
   Size:        111    3207
 
ZraI GAC'GTC

Cuts at:      0    2280    3318 
   Size:       2280    1038
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI     AfeI   AflIII     AhdI     AleI
     AlfI     AloI    AlwNI     ApaI     AvaI    BamHI     BanI    BanII
     BbsI    BbvCI     BcgI     BclI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI
   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI    CspCI     EaeI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FspI    HaeII
    Hin4I   HincII     MmeI     MscI     MslI   MspA1I     NcoI     NspI
     PasI    PflMI     PmlI    PpuMI    PshAI   PspOMI     PstI    PvuII
     SacI    SacII     ScaI    SexAI     SfcI     SmaI     SmlI     SphI
     StuI     StyI    TaqII     TatI     TstI     XcmI     XhoI     XmaI
     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI    AflII     AgeI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI     BbeI    BciVI    BfrBI     BmtI    BsaBI
    BsiEI    BsiWI     BsmI    BsrGI   BssHII   BstAPI    BstBI  BstZ17I
   Bsu36I     ClaI     DraI   DraIII     DrdI     EagI    EcoRI    EcoRV
     FalI     FseI    FspAI  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PciI     PfoI     PmeI     PpiI     PsiI    PspXI
     PsrI     PvuI    RsrII     SalI    SanDI     SapI     SbfI     SfiI
     SfoI     SgfI    SgrAI    SnaBI     SpeI     SrfI     SspI     SwaI
  Tth111I     XbaI     XmnI
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