Restriction map for pF1KB6005
 (Linear) (Six-base) MAPSORT of: KIBB6005.seq  Check: 2420  from: 1  to: 3249



KIBB6005.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:28  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2128    3249 
   Size:       2128    1121
 
AatII G_ACGT'C

Cuts at:      0    2864    3249 
   Size:       2864     385
 
AccI GT'mk_AC

Cuts at:      0     322    1612    3249 
   Size:        322    1290    1637

  Fragments arranged by size: 

                1637    1290     322
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     236     533    1089    1154    1789    1838    1862    3205 
   Size:        236     297     556      65     635      49      24    1343

Cuts at:   3205    3249 
   Size:         44

  Fragments arranged by size: 

                1343     635     556     297     236      65      49      44
                  24
 
AflII C'TTAA_G

Cuts at:      0    3154    3249 
   Size:       3154      95
 
AflIII A'CryG_T

Cuts at:      0    2165    3249 
   Size:       2165    1084
 
AleI CACnn'nnGTG

Cuts at:      0    1120    1885    2954    3249 
   Size:       1120     765    1069     295

  Fragments arranged by size: 

                1120    1069     765     295
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2027    2059    3249 
   Size:       2027      32    1190

  Fragments arranged by size: 

                2027    1190      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     706    1126    2401    2497    3249 
   Size:        706     420    1275      96     752

  Fragments arranged by size: 

                1275     752     706     420      96
 
ApaI G_GGCC'C

Cuts at:      0       7     662    2434    3249 
   Size:          7     655    1772     815

  Fragments arranged by size: 

                1772     815     655       7
 
ApoI r'AATT_y

Cuts at:      0    1769    3249 
   Size:       1769    1480
 
AvaI C'yCGr_G

Cuts at:      0     263     692    2090    3249 
   Size:        263     429    1398    1159

  Fragments arranged by size: 

                1398    1159     429     263
 
AvrII C'CTAG_G

Cuts at:      0    2128    3249 
   Size:       2128    1121
 
BamHI G'GATC_C

Cuts at:      0    1331    1679    3249 
   Size:       1331     348    1570

  Fragments arranged by size: 

                1570    1331     348
 
BanI G'GyrC_C

Cuts at:      0      14    1299    2140    2590    3249 
   Size:         14    1285     841     450     659

  Fragments arranged by size: 

                1285     841     659     450      14
 
BanII G_rGCy'C

Cuts at:      0       7     111     662     894    1990    2434    3249 
   Size:          7     104     551     232    1096     444     815

  Fragments arranged by size: 

                1096     815     551     444     232     104       7
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2692    3249 
   Size:       2692     557
 
BbvCI CC'TCA_GC

Cuts at:      0    1835    2066    3249 
   Size:       1835     231    1183

  Fragments arranged by size: 

                1835    1183     231
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1559    1720    1733    2217    2931    3249 
   Size:       1559     161      13     484     714     318

  Fragments arranged by size: 

                1559     714     484     318     161      13
 
BclI T'GATC_A

Cuts at:      0    2223    3249 
   Size:       2223    1026
 
BfrBI ATG'CAT

Cuts at:      0    1654    3249 
   Size:       1654    1595
 
BlpI GC'TnA_GC

Cuts at:      0     124     782    3249 
   Size:        124     658    2467

  Fragments arranged by size: 

                2467     658     124
 
Bme1580I G_kGCm'C

Cuts at:      0       7     662    1304    2434    3249 
   Size:          7     655     642    1130     815

  Fragments arranged by size: 

                1130     815     655     642       7
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     587    1002    1279    2767    2958    3249 
   Size:        587     415     277    1488     191     291

  Fragments arranged by size: 

                1488     587     415     291     277     191
 
BmtI G_CTAG'C

Cuts at:      0    1967    3249 
   Size:       1967    1282
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     241     884    1907    2360    3249 
   Size:        241     643    1023     453     889

  Fragments arranged by size: 

                1023     889     643     453     241
 
Bpu10I CC'TnA_GC

Cuts at:      0    1562    1835    2066    3249 
   Size:       1562     273     231    1183

  Fragments arranged by size: 

                1562    1183     273     231
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     527    2918    3249 
   Size:        527    2391     331

  Fragments arranged by size: 

                2391     527     331
 
BsaAI yAC'GTr

Cuts at:      0    2166    3249 
   Size:       2166    1083
 
BsaBI GATnn'nnATC

Cuts at:      0    1372    3062    3249 
   Size:       1372    1690     187

  Fragments arranged by size: 

                1690    1372     187
 
BsaHI Gr'CG_yC

Cuts at:      0     385    1063    2861    3249 
   Size:        385     678    1798     388

  Fragments arranged by size: 

                1798     678     388     385
 
BsaWI w'CCGG_w

Cuts at:      0    1378    2012    2181    3249 
   Size:       1378     634     169    1068

  Fragments arranged by size: 

                1378    1068     634     169
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1191    1221    1959    1989    2514    2544    3249 
   Size:       1191      30     738      30     525      30     705

  Fragments arranged by size: 

                1191     738     705     525      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     283    1316    1371    2054    2720    2890    3249 
   Size:        283    1033      55     683     666     170     359

  Fragments arranged by size: 

                1033     683     666     359     283     170      55
 
BseYI C'CCAG_C

Cuts at:      0     177    1304    2255    3249 
   Size:        177    1127     951     994

  Fragments arranged by size: 

                1127     994     951     177
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     331     719    1733    1796    1963    2366    3249 
   Size:        331     388    1014      63     167     403     883

  Fragments arranged by size: 

                1014     883     403     388     331     167      63
 
BsiHKAI G_wGCw'C

Cuts at:      0     236     894    2934    3249 
   Size:        236     658    2040     315

  Fragments arranged by size: 

                2040     658     315     236
 
Bsp1286I G_dGCh'C

Cuts at:      0       7     111     236     662     894    1304    1990    2434 
   Size:          7     104     125     426     232     410     686     444

Cuts at:   2434    2934    3249 
   Size:        500     315

  Fragments arranged by size: 

                 686     500     444     426     410     315     232     125
                 104       7
 
BspEI T'CCGG_A

Cuts at:      0    1378    2012    3249 
   Size:       1378     634    1237

  Fragments arranged by size: 

                1378    1237     634
 
BspHI T'CATG_A

Cuts at:      0     611    2483    3249 
   Size:        611    1872     766

  Fragments arranged by size: 

                1872     766     611
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     501     608    2031    2128    3026    3249 
   Size:        501     107    1423      97     898     223

  Fragments arranged by size: 

                1423     898     501     223     107      97
 
BsrBI CCG'CTC

Cuts at:      0    2344    3249 
   Size:       2344     905
 
BsrDI GCAATG_nn'

Cuts at:      0     458     654    2234    2372    2542    3249 
   Size:        458     196    1580     138     170     707

  Fragments arranged by size: 

                1580     707     458     196     170     138
 
BsrFI r'CCGG_y

Cuts at:      0      25    1716    3249 
   Size:         25    1691    1533

  Fragments arranged by size: 

                1691    1533      25
 
BssSI C'ACGA_G

Cuts at:      0    1013    3249 
   Size:       1013    2236
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     706    1660    3249 
   Size:        706     954    1589

  Fragments arranged by size: 

                1589     954     706
 
BstEII G'GTnAC_C

Cuts at:      0     513    2123    3249 
   Size:        513    1610    1126

  Fragments arranged by size: 

                1610    1126     513
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     561    1404    1887    3249 
   Size:        561     843     483    1362

  Fragments arranged by size: 

                1362     843     561     483
 
BstYI r'GATC_y

Cuts at:      0    1186    1331    1679    3249 
   Size:       1186     145     348    1570

  Fragments arranged by size: 

                1570    1186     348     145
 
Bsu36I CC'TnA_GG

Cuts at:      0     745    3249 
   Size:        745    2504
 
BtgI C'CryG_G

Cuts at:      0      10     524    1409    1937    1996    2195    3249 
   Size:         10     514     885     528      59     199    1054

  Fragments arranged by size: 

                1054     885     528     514     199      59      10
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1640    1976    3249 
   Size:       1640     336    1273

  Fragments arranged by size: 

                1640    1273     336
 
BtsI GCAGTG_nn'

Cuts at:      0     365     398    1865    2687    3249 
   Size:        365      33    1467     822     562

  Fragments arranged by size: 

                1467     822     562     365      33
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     336     371    1919    1954    3249 
   Size:        336      35    1548      35    1295

  Fragments arranged by size: 

                1548    1295     336      35      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    2590    3249 
   Size:       2590     659
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2866    3249 
   Size:       2866     383
 
EaeI y'GGCC_r

Cuts at:      0     826    1406    1889    1999    2174    3249 
   Size:        826     580     483     110     175    1075

  Fragments arranged by size: 

                1075     826     580     483     175     110
 
EarI CTCTTCn'nnn_

Cuts at:      0     671    3249 
   Size:        671    2578
 
EcoICRI GAG'CTC

Cuts at:      0     892    3249 
   Size:        892    2357
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     236     241     533     884    1089    1154    1789    1838 
   Size:        236       5     292     351     205      65     635      49

Cuts at:   1838    1862    1907    2360    3205    3249 
   Size:         24      45     453     845      44

  Fragments arranged by size: 

                 845     635     453     351     292     236     205      65
                  49      45      44      24       5
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     101    1055    2598    3249 
   Size:        101     954    1543     651

  Fragments arranged by size: 

                1543     954     651     101
 
EcoO109I rG'GnC_Cy

Cuts at:      0       4     132     296     659    1730    2431    3249 
   Size:          4     128     164     363    1071     701     818

  Fragments arranged by size: 

                1071     818     701     363     164     128       4
 
EcoRI G'AATT_C

Cuts at:      0    1769    3249 
   Size:       1769    1480
 
EcoRV GAT'ATC

Cuts at:      0    2481    3249 
   Size:       2481     768
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    2191    2223    2668    2700    3249 
   Size:       2191      32     445      32     549

  Fragments arranged by size: 

                2191     549     445      32      32
 
HincII GTy'rAC

Cuts at:      0     323    3249 
   Size:        323    2926
 
HindIII A'AGCT_T

Cuts at:      0     545    3249 
   Size:        545    2704
 
MfeI C'AATT_G

Cuts at:      0    1883    3249 
   Size:       1883    1366
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1566    2272    2302    3249 
   Size:       1566     706      30     947

  Fragments arranged by size: 

                1566     947     706      30
 
MscI TGG'CCA

Cuts at:      0    1408    1891    2176    3249 
   Size:       1408     483     285    1073

  Fragments arranged by size: 

                1408    1073     483     285
 
MslI CAynn'nnrTG

Cuts at:      0     559    1120    1885    2954    3249 
   Size:        559     561     765    1069     295

  Fragments arranged by size: 

                1069     765     561     559     295
 
MspA1I CmG'CkG

Cuts at:      0      12     181     703     762     919    1183    2380    2976 
   Size:         12     169     522      59     157     264    1197     596

Cuts at:   2976    3249 
   Size:        273

  Fragments arranged by size: 

                1197     596     522     273     264     169     157      59
                  12
 
NaeI GCC'GGC

Cuts at:      0      27    1718    3249 
   Size:         27    1691    1531

  Fragments arranged by size: 

                1691    1531      27
 
NcoI C'CATG_G

Cuts at:      0     524    1409    2195    3249 
   Size:        524     885     786    1054

  Fragments arranged by size: 

                1054     885     786     524
 
NgoMIV G'CCGG_C

Cuts at:      0      25    1716    3249 
   Size:         25    1691    1533

  Fragments arranged by size: 

                1691    1533      25
 
NheI G'CTAG_C

Cuts at:      0    1963    3249 
   Size:       1963    1286
 
NsiI A_TGCA'T

Cuts at:      0    1656    3249 
   Size:       1656    1593
 
NspI r_CATG'y

Cuts at:      0     928    1663    2629    3249 
   Size:        928     735     966     620

  Fragments arranged by size: 

                 966     928     735     620
 
PasI CC'CwG_GG

Cuts at:      0    1704    1735    1983    3249 
   Size:       1704      31     248    1266

  Fragments arranged by size: 

                1704    1266     248      31
 
PfoI T'CCnGG_A

Cuts at:      0    2337    3249 
   Size:       2337     912
 
PmlI CAC'GTG

Cuts at:      0    2166    3249 
   Size:       2166    1083
 
PpuMI rG'GwC_Cy

Cuts at:      0    1730    3249 
   Size:       1730    1519
 
PspOMI G'GGCC_C

Cuts at:      0       3     658    2430    3249 
   Size:          3     655    1772     819

  Fragments arranged by size: 

                1772     819     655       3
 
PspXI vC'TCGA_Gb

Cuts at:      0     692    2090    3249 
   Size:        692    1398    1159

  Fragments arranged by size: 

                1398    1159     692
 
PstI C_TGCA'G

Cuts at:      0     984    1131    2139    2502    2897    3023    3249 
   Size:        984     147    1008     363     395     126     226

  Fragments arranged by size: 

                1008     984     395     363     226     147     126
 
PvuII CAG'CTG

Cuts at:      0     181     703     762    1183    2380    2976    3249 
   Size:        181     522      59     421    1197     596     273

  Fragments arranged by size: 

                1197     596     522     421     273     181      59
 
SacI G_AGCT'C

Cuts at:      0     894    3249 
   Size:        894    2355
 
SacII CC_GC'GG

Cuts at:      0      13    3249 
   Size:         13    3236
 
SalI G'TCGA_C

Cuts at:      0     321    3249 
   Size:        321    2928
 
SanDI GG'GwC_CC

Cuts at:      0    1730    3249 
   Size:       1730    1519
 
SbfI CC_TGCA'GG

Cuts at:      0    2139    3023    3249 
   Size:       2139     884     226

  Fragments arranged by size: 

                2139     884     226
 
ScaI AGT'ACT

Cuts at:      0    1110    3249 
   Size:       1110    2139
 
SexAI A'CCwGG_T

Cuts at:      0    2586    3249 
   Size:       2586     663
 
SfcI C'TryA_G

Cuts at:      0      67     185     980    1127    2135    2498    2893    2971 
   Size:         67     118     795     147    1008     363     395      78

Cuts at:   2971    3019    3249 
   Size:         48     230

  Fragments arranged by size: 

                1008     795     395     363     230     147     118      78
                  67      48
 
SmaI CCC'GGG

Cuts at:      0     265    3249 
   Size:        265    2984
 
SmlI C'TyrA_G

Cuts at:      0     542     692    2090    2933    3154    3249 
   Size:        542     150    1398     843     221      95

  Fragments arranged by size: 

                1398     843     542     221     150      95
 
SphI G_CATG'C

Cuts at:      0     928    1663    3249 
   Size:        928     735    1586

  Fragments arranged by size: 

                1586     928     735
 
SspI AAT'ATT

Cuts at:      0    1459    3249 
   Size:       1459    1790
 
StuI AGG'CCT

Cuts at:      0    2133    3249 
   Size:       2133    1116
 
StyI C'CwwG_G

Cuts at:      0     497     524    1292    1409    2128    2195    2599    2735 
   Size:        497      27     768     117     719      67     404     136

Cuts at:   2735    3249 
   Size:        514

  Fragments arranged by size: 

                 768     719     514     497     404     136     117      67
                  27
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1707    2376    2395    3249 
   Size:       1707     669      19     854

  Fragments arranged by size: 

                1707     854     669      19
 
TatI w'GTAC_w

Cuts at:      0     792    1108    3249 
   Size:        792     316    2141

  Fragments arranged by size: 

                2141     792     316
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     226     258    1191    1223    1959    1991    2514    2546 
   Size:        226      32     933      32     736      32     523      32

Cuts at:   2546    3249 
   Size:        703

  Fragments arranged by size: 

                 933     736     703     523     226      32      32      32
                  32
 
Tth111I GACn'n_nGTC

Cuts at:      0    3178    3249 
   Size:       3178      71
 
XbaI T'CTAG_A

Cuts at:      0    2985    3249 
   Size:       2985     264
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2034    2700    3249 
   Size:       2034     666     549

  Fragments arranged by size: 

                2034     666     549
 
XhoI C'TCGA_G

Cuts at:      0     692    2090    3249 
   Size:        692    1398    1159

  Fragments arranged by size: 

                1398    1159     692
 
XmaI C'CCGG_G

Cuts at:      0     263    3249 
   Size:        263    2986
 
XmnI GAAnn'nnTTC

Cuts at:      0    1851    3249 
   Size:       1851    1398
 
ZraI GAC'GTC

Cuts at:      0    2862    3249 
   Size:       2862     387
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI    AflII   AflIII     AleI     AloI
    AlwNI     ApaI     ApoI     AvaI    AvrII    BamHI     BanI    BanII
     BbsI    BbvCI    BciVI     BclI    BfrBI     BlpI Bme1580I     BmrI
     BmtI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI   BstEII    BstXI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI    CspCI   DraIII     DrdI
     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
    Hin4I   HincII  HindIII     MfeI     MmeI     MscI     MslI   MspA1I
     NaeI     NcoI   NgoMIV     NheI     NsiI     NspI     PasI     PfoI
     PmlI    PpuMI   PspOMI    PspXI     PstI    PvuII     SacI    SacII
     SalI    SanDI     SbfI     ScaI    SexAI     SfcI     SmaI     SmlI
     SphI     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AfeI     AgeI     AhdI     AlfI    ApaLI     AscI
     AseI    AsiSI     BaeI     BbeI     BcgI     BglI    BglII    BmgBI
     BplI     BsaI    BsiEI    BsiWI     BsmI    BsmBI    BsrGI   BssHII
    BstBI  BstZ17I     ClaI     DraI     EagI     EciI     FalI     FseI
     FspI    FspAI    HaeII     HpaI     KasI     KpnI     MluI     NarI
     NdeI     NotI     NruI     PacI     PciI    PflMI     PmeI     PpiI
    PshAI     PsiI     PsrI     PvuI    RsrII     SapI     SfiI     SfoI
     SgfI    SgrAI    SnaBI     SpeI     SrfI     SwaI
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