Restriction map for pF1KB5702
##################################
# NO CUT ENZYMES (   99 enzymes) #
##################################

AatII       Acc65I      AccIII      AcyI        AflII       
AgeI        AhdI        Alw44I      ApaLI       AscI        
Asp718I     AspEI       AspHI       AspI        AviII       
AvrII       BbeI        BcgI        BciVI       BfrI        
BglII       BlnI        BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsiEI       BsiHKAI     BsiWI       
BsmBI       Bsp1407I    BspEI       BspHI       BspLU11I    
BsrBI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstEII      BstPI       BstXI       BstZ17I     
Cfr10I      CpoI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco52I      EcoO65I     EcoRI       
EcoRV       EcoT22I     FseI        FspI        Hin1I       
HincII      HindII      HpaI        KasI        KpnI        
KspI        MamI        MfeI        MroI        MunI        
NaeI        NarI        NgoMIV      NheI        NotI        
NruI        NsiI        PacI        PflFI       PinAI       
PmeI        PvuI        RcaI        RsrII       SacI        
SacII       SalI        ScaI        SexAI       SfiI        
SfoI        SgrAI       SnaBI       SpeI        SspBI       
SspI        SwaI        Tth111I     XcmI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccII         974  CGCG                 CG^CG
AclI          649  AACGTT               AA^CGTT
AcsI          210  GAATTT               [GA]^AATT[CT]
AfaI         1050  GTAC                 GT^AC
AflIII        973  ACGCGT               A^C[GA][CT]GT
Aor51HI       896  AGCGCT               AGC^GCT
ApaI          136  GGGCCC               GGGCC^C
ApoI          210  GAATTT               [GA]^AATT[CT]
AseI         1393  ATTAAT               AT^TAAT
AsnI         1393  ATTAAT               AT^TAAT
Asp700I       283  GAAGATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI         2586  GGCACC               G^G[CT][GA]CC
BanII         136  GGGCCC               G[GA]GC[CT]^C
BbrPI        2668  CACGTG               CAC^GTG
BclI          612  TGATCA               T^GATCA
BlpI         2020  GCTCAGC              GC^T[ACGT]AGC
BpmI          913  CTGGAG               CTGGAG(16/14)
Bpu1102I     2020  GCTCAGC              GC^T[ACGT]AGC
BspDI        1834  ATCGAT               AT^CGAT
BstUI         974  CGCG                 CG^CG
CelII        2020  GCTCAGC              GC^T[ACGT]AGC
ClaI         1834  ATCGAT               AT^CGAT
DraI         1459  TTTAAA               TTT^AAA
Eco47III      896  AGCGCT               AGC^GCT
EcoNI        2451  CCTGATCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I      1814  CCATGG               C^C[AT][AT]GG
FbaI          612  TGATCA               T^GATCA
MluI          973  ACGCGT               A^CGCGT
MvnI          974  CGCG                 CG^CG
NcoI         1814  CCATGG               C^CATGG
NdeI          836  CATATG               CA^TATG
PaeR7I        383  CTCGAG               C^TCGAG
PmaCI        2668  CACGTG               CAC^GTG
PmlI         2668  CACGTG               CAC^GTG
PshBI        1393  ATTAAT               AT^TAAT
Psp1406I      649  AACGTT               AA^CGTT
RsaI         1050  GTAC                 GT^AC
SapI          347  GCTCTTC              GCTCTTC(1/4)
SbfI         1029  CCTGCAGG             CCTGCA^GG
SphI          679  GCATGC               GCATG^C
Sse8387I     1029  CCTGCAGG             CCTGCA^GG
StuI          904  AGGCCT               AGG^CCT
StyI         1814  CCATGG               C^C[AT][AT]GG
XbaI           51  TCTAGA               T^CTAGA
XhoI          383  CTCGAG               C^TCGAG
XmnI          283  GAAGATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AocI         1384  CCTCAGG              CC^T[ACGT]AGG
             1903  CCTGAGG             
BamHI          39  GGATCC               G^GATCC
             2257  GGATCC              
BbsI         1119  GAAGAC               GAAGAC(2/6)
             1975  GAAGAC              
BfaI           52  CTAG                 C^TAG
              629  CTAG                
BmyI          136  GGGCCC               G[AGT]GC[ACT]^C
             2003  GTGCCC              
BpuAI        1119  GAAGAC               GAAGAC(2/6)
             1975  GAAGAC              
BsaAI        1660  CACGTA               [CT]AC^GT[GA]
             2668  CACGTG              
BsmI          495  GAATGC               GAATGC(1/-1)
             2478  GAATGC              
Bsp1286I      136  GGGCCC               G[AGT]GC[ACT]^C
             2003  GTGCCC              
BstAPI       1032  GCAGGACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2319  GCAGGACCTGC         
BstBI         290  TTCGAA               TT^CGAA
             1349  TTCGAA              
Bsu36I       1384  CCTCAGG              CC^T[ACGT]AGG
             1903  CCTGAGG             
CfoI          897  GCGC                 GCG^C
             2441  GCGC                
DsaI         1781  CCGTGG               C^C[GA][CT]GG
             1814  CCATGG              
Eco81I       1384  CCTCAGG              CC^T[ACGT]AGG
             1903  CCTGAGG             
FauI         1844  CCCGC                CCCGC(4/6)
             2411  GCGGG               
HaeII         896  AGCGCT               [GA]GCGC^[CT]
             2440  AGCGCC              
HgaI          921  GCGTC                GACGC(5/10)
             1964  GCGTC               
HhaI          897  GCGC                 GCG^C
             2441  GCGC                
HinP1I        897  GCGC                 G^CGC
             2441  GCGC                
HindIII      1342  AAGCTT               A^AGCTT
             2272  AAGCTT              
MaeI           52  CTAG                 C^TAG
              629  CTAG                
NspI          679  GCATGC               [GA]CATG^[CT]
             1275  ACATGC              
NspV          290  TTCGAA               TT^CGAA
             1349  TTCGAA              
PflMI         174  CCATGTTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1809  CCAGCCCATGG         
PshAI           3  GACTGCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             1959  GACGAGCGTC          
SfuI          290  TTCGAA               TT^CGAA
             1349  TTCGAA              
SmaI         1649  CCCGGG               CCC^GGG
             2006  CCCGGG              
Van91I        174  CCATGTTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1809  CCAGCCCATGG         
XmaCI        1649  CCCGGG               C^CCGGG
             2006  CCCGGG              
XmaI         1649  CCCGGG               C^CCGGG
             2006  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   82 enzymes) #
#########################################

AccI        AciI        AluI        AlwI        AlwNI       
AvaI        AvaII       BalI        BbvCI       BbvI        
BcnI        BglI        BsaJI       BseRI       BsgI        
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
BspMI       BsrDI       BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       DraII       EaeI        EarI        EcoO109I    
EcoRII      Fnu4HI      FokI        HaeIII      HapII       
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        PpuMI       
PstI        PvuII       Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XhoII       
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