Restriction map for pF1KB5697
##################################
# NO CUT ENZYMES (   95 enzymes) #
##################################

AatII       AccIII      AclI        AflII       AflIII      
AhdI        Alw44I      Aor51HI     ApaLI       AscI        
AseI        AsnI        AspEI       AspI        AviII       
AvrII       BbeI        BbvCI       BcgI        BfrI        
BlnI        BsaBI       BseAI       BsiWI       BsmBI       
Bsp1407I    BspDI       BspEI       BspLU11I    BsrBI       
BsrDI       BsrGI       BstAPI      BstBI       BstEII      
BstPI       ClaI        CpoI        DraI        DraIII      
Eam1105I    Eco47III    EcoO65I     EcoRI       EcoRV       
EcoT22I     FauI        FseI        FspI        HincII      
HindII      HindIII     HpaI        KasI        KspI        
MamI        MfeI        MluI        MroI        MunI        
NarI        NdeI        NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacII       SalI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmlI        
SnaBI       SpeI        SphI        SspBI       SspI        
StuI        SwaI        Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   63 enzymes) #
###################################

Acc65I       1652  GGTACC               G^GTACC
AccI         1567  GTATAC               GT^[AC][GT]AC
AcsI         1759  AAATTC               [GA]^AATT[CT]
AcyI         1491  GACGCC               G[GA]^CG[CT]C
AgeI         1469  ACCGGT               A^CCGGT
AlwNI         590  CAGGCACTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI           39  CCTGAGG              CC^T[ACGT]AGG
ApoI         1759  AAATTC               [GA]^AATT[CT]
Asp700I       162  GAACACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1652  GGTACC               G^GTACC
AvaI           17  CCCGGG               C^[CT]CG[GA]G
BamHI        1794  GGATCC               G^GATCC
BbrPI        1496  CACGTG               CAC^GTG
BciVI         541  GGATAC               GTATCC(6/5)
BclI          509  TGATCA               T^GATCA
BfaI         1972  CTAG                 C^TAG
BlpI           69  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I       69  GCTGAGC              GC^T[ACGT]AGC
BsaAI        1496  CACGTG               [CT]AC^GT[GA]
BsaHI        1491  GACGCC               G[GA]^CG[CT]C
BsaWI        1469  ACCGGT               [AT]^CCGG[AT]
BseRI        1585  GAGGAG               GAGGAG(10/8)
BsoBI          17  CCCGGG               C^[CT]CG[GA]G
BspHI         506  TCATGA               T^CATGA
BspMI        1865  ACCTGC               ACCTGC(4/8)
BssHII         23  GCGCGC               G^CGCGC
Bst1107I     1567  GTATAC               GTA^TAC
BstZ17I      1567  GTATAC               GTA^TAC
Bsu36I         39  CCTGAGG              CC^T[ACGT]AGG
CelII          69  GCTGAGC              GC^T[ACGT]AGC
DrdI         1002  GACCGCCATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI         1935  GAAGAG               CTCTTC(1/4)
Eco81I         39  CCTGAGG              CC^T[ACGT]AGG
EcoNI        1171  CCTGACCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI          509  TGATCA               T^GATCA
HgaI         1491  GACGC                GACGC(5/10)
Hin1I        1491  GACGCC               G[GA]^CG[CT]C
KpnI         1652  GGTACC               GGTAC^C
Ksp632I      1935  GAAGAG               CTCTTC(1/4)
MaeI         1972  CTAG                 C^TAG
MaeII        1497  ACGT                 A^CGT
MseI         1715  TTAA                 T^TAA
NaeI         1096  GCCGGC               GCC^GGC
NgoMIV       1096  GCCGGC               G^CCGGC
PinAI        1469  ACCGGT               A^CCGGT
PmaCI        1496  CACGTG               CAC^GTG
PmlI         1496  CACGTG               CAC^GTG
PpuMI          33  GGGTCCC              [GA]G^G[AT]CC[CT]
PstI         1867  CTGCAG               CTGCA^G
RcaI          506  TCATGA               T^CATGA
SapI         1935  GAAGAGC              GCTCTTC(1/4)
SbfI         1866  CCTGCAGG             CCTGCA^GG
ScaI          692  AGTACT               AGT^ACT
SfcI         1867  CTGCAG               C^T[GA][CT]AG
SmaI           17  CCCGGG               CCC^GGG
Sse8387I     1866  CCTGCAGG             CCTGCA^GG
TfiI          482  GAATC                G^A[AT]TC
Tru9I        1715  TTAA                 T^TAA
Tsp509I      1760  AATT                 ^AATT
XcmI         1370  CCACCCCCACGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI          17  CCCGGG               C^CCGGG
XmaI           17  CCCGGG               C^CCGGG
XmnI          162  GAACACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

BbsI         1734  GAAGAC               GAAGAC(2/6)
             1048  GTCTTC              
BglI         1255  GCCCTGATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1506  GCCCATGGGGC         
BglII        1400  AGATCT               A^GATCT
             1451  AGATCT              
BpuAI        1734  GAAGAC               GAAGAC(2/6)
             1048  GTCTTC              
BsaI          283  GAGACC               GGTCTC(1/5)
             1000  GAGACC              
BsmI         1830  GAATGC               GAATGC(1/-1)
             1319  GCATTC              
EagI          750  CGGCCG               C^GGCCG
             1098  CGGCCG              
EclXI         750  CGGCCG               C^GGCCG
             1098  CGGCCG              
Eco52I        750  CGGCCG               C^GGCCG
             1098  CGGCCG              
MslI          532  CATGGCGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              616  CATTTTGATG          
PleI          566  GACTC                GAGTC(4/5)
              610  GACTC               
PvuII         735  CAGCTG               CAG^CTG
              909  CAGCTG              
SacI         1327  GAGCTC               GAGCT^C
             1543  GAGCTC              
TaqI          281  TCGA                 T^CGA
              480  TCGA                
TthHB8I       281  TCGA                 T^CGA
              480  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   85 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
ApaI        AspHI       AvaII       BalI        BanI        
BanII       BbvI        BcnI        BmyI        BpmI        
BsaJI       BsgI        BsiEI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BsrFI       
BsrI        BssKI       BssSI       BstF5I      BstNI       
BstUI       BstXI       BstYI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EcoO109I    EcoRII      EcoT14I     
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NcoI        
NdeII       NlaIII      NlaIV       PflMI       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       StyI        
TseI        Tsp45I      TspRI       Van91I      XhoII       
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