Restriction map for pF1KB5697
 (Linear) (Six-base) MAPSORT of: KIBB5697.seq  Check: 5097  from: 1  to: 1980



KIBB5697.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:28  ..

AccI GT'mk_AC

Cuts at:      0    1568    1980 
   Size:       1568     412
 
Acc65I G'GTAC_C

Cuts at:      0    1652    1980 
   Size:       1652     328
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     229     878    1933    1954    1980 
   Size:        229     649    1055      21      26

  Fragments arranged by size: 

                1055     649     229      26      21
 
AgeI A'CCGG_T

Cuts at:      0    1469    1980 
   Size:       1469     511
 
AlwNI CAG_nnn'CTG

Cuts at:      0     595    1980 
   Size:        595    1385
 
ApaI G_GGCC'C

Cuts at:      0    1257    1387    1517    1980 
   Size:       1257     130     130     463

  Fragments arranged by size: 

                1257     463     130     130
 
ApoI r'AATT_y

Cuts at:      0    1759    1980 
   Size:       1759     221
 
AvaI C'yCGr_G

Cuts at:      0      17    1980 
   Size:         17    1963
 
BamHI G'GATC_C

Cuts at:      0    1794    1980 
   Size:       1794     186
 
BanI G'GyrC_C

Cuts at:      0     952    1036    1263    1360    1652    1900    1980 
   Size:        952      84     227      97     292     248      80

  Fragments arranged by size: 

                 952     292     248     227      97      84      80
 
BanII G_rGCy'C

Cuts at:      0    1257    1331    1387    1517    1547    1980 
   Size:       1257      74      56     130      30     433

  Fragments arranged by size: 

                1257     433     130      74      56      30
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1041    1741    1980 
   Size:       1041     700     239

  Fragments arranged by size: 

                1041     700     239
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     535    1980 
   Size:        535    1445
 
BclI T'GATC_A

Cuts at:      0     509    1980 
   Size:        509    1471
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1261    1512    1980 
   Size:       1261     251     468

  Fragments arranged by size: 

                1261     468     251
 
BglII A'GATC_T

Cuts at:      0    1400    1451    1980 
   Size:       1400      51     529

  Fragments arranged by size: 

                1400     529      51
 
BlpI GC'TnA_GC

Cuts at:      0      70    1980 
   Size:         70    1910
 
Bme1580I G_kGCm'C

Cuts at:      0     898    1041    1257    1387    1517    1980 
   Size:        898     143     216     130     130     463

  Fragments arranged by size: 

                 898     463     216     143     130     130
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     471    1252    1663    1740    1772    1785    1939    1980 
   Size:        471     781     411      77      32      13     154      41

  Fragments arranged by size: 

                 781     471     411     154      77      41      32      13
            
 
Bpu10I CC'TnA_GC

Cuts at:      0    1541    1980 
   Size:       1541     439
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     277     994    1980 
   Size:        277     717     986

  Fragments arranged by size: 

                 986     717     277
 
BsaAI yAC'GTr

Cuts at:      0    1498    1980 
   Size:       1498     482
 
BsaHI Gr'CG_yC

Cuts at:      0    1492    1980 
   Size:       1492     488
 
BsaWI w'CCGG_w

Cuts at:      0    1469    1980 
   Size:       1469     511
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1636    1666    1980 
   Size:       1636      30     314

  Fragments arranged by size: 

                1636     314      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1600    1980 
   Size:       1600     380
 
BseYI C'CCAG_C

Cuts at:      0     524    1380    1980 
   Size:        524     856     600

  Fragments arranged by size: 

                 856     600     524
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     723     897    1071    1980 
   Size:        723     174     174     909

  Fragments arranged by size: 

                 909     723     174     174
 
BsiEI CG_ry'CG

Cuts at:      0     753     972    1101    1152    1980 
   Size:        753     219     129      51     828

  Fragments arranged by size: 

                 828     753     219     129      51
 
BsiHKAI G_wGCw'C

Cuts at:      0     778    1238    1331    1547    1980 
   Size:        778     460      93     216     433

  Fragments arranged by size: 

                 778     460     433     216      93
 
BsmI GAATG_Cn'

Cuts at:      0    1319    1836    1980 
   Size:       1319     517     144

  Fragments arranged by size: 

                1319     517     144
 
Bsp1286I G_dGCh'C

Cuts at:      0     778     898    1041    1238    1257    1331    1387    1517 
   Size:        778     120     143     197      19      74      56     130

Cuts at:   1517    1547    1980 
   Size:         30     433

  Fragments arranged by size: 

                 778     433     197     143     130     120      74      56
                  30      19
 
BspHI T'CATG_A

Cuts at:      0     506    1980 
   Size:        506    1474
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1874    1980 
   Size:       1874     106
 
BsrFI r'CCGG_y

Cuts at:      0     748    1096    1363    1469    1980 
   Size:        748     348     267     106     511

  Fragments arranged by size: 

                 748     511     348     267     106
 
BssHII G'CGCG_C

Cuts at:      0      23    1980 
   Size:         23    1957
 
BssSI C'ACGA_G

Cuts at:      0     434    1195    1207    1980 
   Size:        434     761      12     773

  Fragments arranged by size: 

                 773     761     434      12
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1213    1230    1249    1641    1980 
   Size:       1213      17      19     392     339

  Fragments arranged by size: 

                1213     392     339      19      17
 
BstYI r'GATC_y

Cuts at:      0    1400    1439    1451    1622    1794    1980 
   Size:       1400      39      12     171     172     186

  Fragments arranged by size: 

                1400     186     172     171      39      12
 
BstZ17I GTA'TAC

Cuts at:      0    1569    1980 
   Size:       1569     411
 
Bsu36I CC'TnA_GG

Cuts at:      0      40    1980 
   Size:         40    1940
 
BtgI C'CryG_G

Cuts at:      0     842    1223    1443    1508    1980 
   Size:        842     381     220      65     472

  Fragments arranged by size: 

                 842     472     381     220      65
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     131     470     551     773    1416    1800    1980 
   Size:        131     339      81     222     643     384     180

  Fragments arranged by size: 

                 643     384     339     222     180     131      81
 
BtsI GCAGTG_nn'

Cuts at:      0     155     466    1110    1980 
   Size:        155     311     644     870

  Fragments arranged by size: 

                 870     644     311     155
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1008    1980 
   Size:       1008     972
 
EaeI y'GGCC_r

Cuts at:      0     320     519     750    1098    1215    1526    1980 
   Size:        320     199     231     348     117     311     454

  Fragments arranged by size: 

                 454     348     320     311     231     199     117
 
EagI C'GGCC_G

Cuts at:      0     750    1098    1980 
   Size:        750     348     882

  Fragments arranged by size: 

                 882     750     348
 
EarI CTCTTCn'nnn_

Cuts at:      0    1930    1980 
   Size:       1930      50
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1497    1980 
   Size:       1497     483
 
EcoICRI GAG'CTC

Cuts at:      0    1329    1545    1980 
   Size:       1329     216     435

  Fragments arranged by size: 

                1329     435     216
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     229     471     878    1252    1663    1740    1772    1785 
   Size:        229     242     407     374     411      77      32      13

Cuts at:   1785    1933    1939    1954    1980 
   Size:        148       6      15      26

  Fragments arranged by size: 

                 411     407     374     242     229     148      77      32
                  26      15      13       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1175    1980 
   Size:       1175     805
 
EcoO109I rG'GnC_Cy

Cuts at:      0      34    1253    1254    1337    1384    1513    1514    1575 
   Size:         34    1219       1      83      47     129       1      61

Cuts at:   1575    1980 
   Size:        405

  Fragments arranged by size: 

                1219     405     129      83      61      47      34       1
                   1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     723     831    1005    1179    1980 
   Size:        723     108     174     174     801

  Fragments arranged by size: 

                 801     723     174     174     108
 
HaeII r_GCGC'y

Cuts at:      0      10      49    1097    1980 
   Size:         10      39    1048     883

  Fragments arranged by size: 

                1048     883      39      10
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     246     278    1980 
   Size:        246      32    1702

  Fragments arranged by size: 

                1702     246      32
 
KpnI G_GTAC'C

Cuts at:      0    1656    1980 
   Size:       1656     324
 
MscI TGG'CCA

Cuts at:      0     322    1217    1528    1980 
   Size:        322     895     311     452

  Fragments arranged by size: 

                 895     452     322     311
 
MslI CAynn'nnrTG

Cuts at:      0     536     620    1980 
   Size:        536      84    1360

  Fragments arranged by size: 

                1360     536      84
 
MspA1I CmG'CkG

Cuts at:      0     524     737     911     944    1980 
   Size:        524     213     174      33    1036

  Fragments arranged by size: 

                1036     524     213     174      33
 
NaeI GCC'GGC

Cuts at:      0    1098    1980 
   Size:       1098     882
 
NcoI C'CATG_G

Cuts at:      0     842    1223    1508    1980 
   Size:        842     381     285     472

  Fragments arranged by size: 

                 842     472     381     285
 
NgoMIV G'CCGG_C

Cuts at:      0    1096    1980 
   Size:       1096     884
 
PasI CC'CwG_GG

Cuts at:      0    1249    1980 
   Size:       1249     731
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     595    1224    1535    1701    1980 
   Size:        595     629     311     166     279

  Fragments arranged by size: 

                 629     595     311     279     166
 
PfoI T'CCnGG_A

Cuts at:      0    1547    1980 
   Size:       1547     433
 
PmlI CAC'GTG

Cuts at:      0    1498    1980 
   Size:       1498     482
 
PpuMI rG'GwC_Cy

Cuts at:      0      34    1980 
   Size:         34    1946
 
PspOMI G'GGCC_C

Cuts at:      0    1253    1383    1513    1980 
   Size:       1253     130     130     467

  Fragments arranged by size: 

                1253     467     130     130
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     685     717    1174    1206    1980 
   Size:        685      32     457      32     774

  Fragments arranged by size: 

                 774     685     457      32      32
 
PstI C_TGCA'G

Cuts at:      0    1871    1980 
   Size:       1871     109
 
PvuII CAG'CTG

Cuts at:      0     737     911    1980 
   Size:        737     174    1069

  Fragments arranged by size: 

                1069     737     174
 
SacI G_AGCT'C

Cuts at:      0    1331    1547    1980 
   Size:       1331     216     433

  Fragments arranged by size: 

                1331     433     216
 
SanDI GG'GwC_CC

Cuts at:      0      34    1980 
   Size:         34    1946
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1930    1980 
   Size:       1930      50
 
SbfI CC_TGCA'GG

Cuts at:      0    1871    1980 
   Size:       1871     109
 
ScaI AGT'ACT

Cuts at:      0     694    1980 
   Size:        694    1286
 
SfcI C'TryA_G

Cuts at:      0    1867    1980 
   Size:       1867     113
 
SmaI CCC'GGG

Cuts at:      0      19    1980 
   Size:         19    1961
 
StyI C'CwwG_G

Cuts at:      0     182     641     842    1176    1223    1356    1508    1980 
   Size:        182     459     201     334      47     133     152     472

  Fragments arranged by size: 

                 472     459     334     201     182     152     133      47
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1281    1980 
   Size:       1281     699
 
TatI w'GTAC_w

Cuts at:      0     692     728    1980 
   Size:        692      36    1252

  Fragments arranged by size: 

                1252     692      36
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1377    1980 
   Size:       1377     603
 
XmaI C'CCGG_G

Cuts at:      0      17    1980 
   Size:         17    1963
 
XmnI GAAnn'nnTTC

Cuts at:      0     166    1980 
   Size:        166    1814
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI     AgeI    AlwNI     ApaI     ApoI     AvaI
    BamHI     BanI    BanII     BbsI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I     BpmI   Bpu10I     BsaI    BsaAI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspHI    BspMI    BsrFI   BssHII    BssSI    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI    HaeII    Hin4I
     KpnI     MscI     MslI   MspA1I     NaeI     NcoI   NgoMIV     PasI
    PflMI     PfoI     PmlI    PpuMI   PspOMI     PsrI     PstI    PvuII
     SacI    SanDI     SapI     SbfI     ScaI     SfcI     SmaI     StyI
    TaqII     TatI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI    AflII   AflIII     AhdI     AleI
     AlfI     AloI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
     BbeI    BbvCI     BcgI    BfrBI    BmgBI     BmrI     BmtI     BplI
    BpuEI    BsaBI    BsiWI    BsmBI    BspEI    BsrBI    BsrDI    BsrGI
   BstAPI    BstBI   BstEII     ClaI    CspCI     DraI   DraIII    EcoRI
    EcoRV     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI
     MfeI     MluI     MmeI     NarI     NdeI     NheI     NotI     NruI
     NsiI     NspI     PacI     PciI     PmeI     PpiI    PshAI     PsiI
    PspXI     PvuI    RsrII    SacII     SalI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     TstI  Tth111I     XbaI     XhoI     ZraI
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