################################## # NO CUT ENZYMES ( 94 enzymes) # ################################## AatII Acc65I AccI AclI AcyI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AviII AvrII BamHI BbeI BbrPI BcgI BciVI BglI BglII BlnI BsaBI BsaHI BsgI BsiWI BspDI BspHI BspLU11I BspMI BsrDI BssHII Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I ClaI CpoI DraI DrdI Eco47III Eco81I EcoO65I EcoRV EcoT22I FseI FspI Hin1I KasI KpnI KspI MamI MfeI MunI NarI NheI NotI NruI NsiI NspV PacI PflMI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SmaI SnaBI SphI Sse8387I SspI StuI SwaI TfiI Van91I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 68 enzymes) # ################################### AccIII 929 TCCGGA T^CCGGA AflIII 161 ACGCGT A^C[GA][CT]GT AgeI 1891 ACCGGT A^CCGGT AhdI 1464 GACAATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 1490 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 103 GGGCCC GGGCC^C Asp700I 1774 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 1464 GACAATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1295 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 1789 CTCGAG C^[CT]CG[GA]G BalI 1073 TGGCCA TGG^CCA BanI 1321 GGTGCC G^G[CT][GA]CC BbsI 598 GAAGAC GAAGAC(2/6) BbvCI 1558 CCTCAGC CCTCAGC(-5/-2) BclI 137 TGATCA T^GATCA BlpI 1102 GCTCAGC GC^T[ACGT]AGC BpmI 1796 CTCCAG CTGGAG(16/14) Bpu1102I 1102 GCTCAGC GC^T[ACGT]AGC BpuAI 598 GAAGAC GAAGAC(2/6) BsaAI 40 TACGTG [CT]AC^GT[GA] BseAI 929 TCCGGA T^CCGGA BsiEI 98 CGGCCG CG[GA][CT]^CG BsmI 479 GAATGC GAATGC(1/-1) BsoBI 1789 CTCGAG C^[CT]CG[GA]G BspEI 929 TCCGGA T^CCGGA BsrBI 147 CCGCTC CCGCTC(-3/-3) BssSI 1421 CACGAG CACGAG(-5/-1) BstAPI 1489 GCAGCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 624 GGATCT [GA]^GATC[CT] CelII 1102 GCTCAGC GC^T[ACGT]AGC DraII 47 GGGACCT [GA]G^G[ACGT]CC[CT] DraIII 1436 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 26 CCATGG C^C[GA][CT]GG EagI 98 CGGCCG C^GGCCG Eam1105I 1464 GACAATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1114 GAAGAG CTCTTC(1/4) EclXI 98 CGGCCG C^GGCCG Eco52I 98 CGGCCG C^GGCCG EcoNI 1210 CCTCCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 47 GGGACCT [GA]G^G[ACGT]CC[CT] EcoRI 565 GAATTC G^AATTC FauI 143 CCCGC CCCGC(4/6) FbaI 137 TGATCA T^GATCA HindIII 708 AAGCTT A^AGCTT HpaI 1879 GTTAAC GTT^AAC Ksp632I 1114 GAAGAG CTCTTC(1/4) MflI 624 GGATCT [GA]^GATC[CT] MluI 161 ACGCGT A^CGCGT MluNI 1073 TGGCCA TGG^CCA MroI 929 TCCGGA T^CCGGA MscI 1073 TGGCCA TGG^CCA NaeI 183 GCCGGC GCC^GGC NcoI 26 CCATGG C^CATGG NdeI 1783 CATATG CA^TATG NgoMIV 183 GCCGGC G^CCGGC NspI 1748 GCATGT [GA]CATG^[CT] PaeR7I 1789 CTCGAG C^TCGAG PflFI 1295 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 1891 ACCGGT A^CCGGT PpuMI 47 GGGACCT [GA]G^G[AT]CC[CT] SapI 1114 GAAGAGC GCTCTTC(1/4) ScaI 1390 AGTACT AGT^ACT SgrAI 1890 CACCGGTG C[GA]^CCGG[CT]G SpeI 961 ACTAGT A^CTAGT Tth111I 1295 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 1789 CTCGAG C^TCGAG XhoII 624 GGATCT [GA]^GATC[CT] XmnI 1774 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccII 162 CGCG CG^CG 192 CGCG AspHI 763 GAGCTC G[AT]GC[AT]^C 794 GAGCTC AvaII 48 GGACC G^G[AT]CC 1471 GGTCC BsaI 1398 GAGACC GGTCTC(1/5) 257 GGTCTC BsaWI 929 TCCGGA [AT]^CCGG[AT] 1891 ACCGGT BseRI 1020 CTCCTC GAGGAG(10/8) 1661 CTCCTC BsiHKAI 763 GAGCTC G[AT]GC[AT]^C 794 GAGCTC BsmBI 925 CGTCTC CGTCTC(1/5) 436 GAGACG BsmFI 47 GGGAC GGGAC(10/14) 131 GGGAC Bsp1407I 426 TGTACA T^GTACA 1614 TGTACA BsrFI 183 GCCGGC [GA]^CCGG[CT] 1891 ACCGGT BsrGI 426 TGTACA T^GTACA 1614 TGTACA BstNI 873 CCAGG CC^[AT]GG 1122 CCAGG BstUI 162 CGCG CG^CG 192 CGCG Cfr10I 183 GCCGGC [GA]^CCGG[CT] 1891 ACCGGT EaeI 98 CGGCCG [CT]^GGCC[GA] 1073 TGGCCA EcoRII 873 CCAGG ^CC[AT]GG 1122 CCAGG HaeII 614 AGCGCC [GA]GCGC^[CT] 1118 AGCGCC HgaI 190 GACGC GACGC(5/10) 438 GACGC HincII 1431 GTCAAC GT[CT]^[GA]AC 1879 GTTAAC HindII 1431 GTCAAC GT[CT]^[GA]AC 1879 GTTAAC MvaI 873 CCAGG CC^[AT]GG 1122 CCAGG MvnI 162 CGCG CG^CG 192 CGCG SacI 763 GAGCTC GAGCT^C 794 GAGCTC SspBI 426 TGTACA T^GTACA 1614 TGTACA TaqI 792 TCGA T^CGA 1790 TCGA Tsp45I 64 GTGAC ^GT[GC]AC 1803 GTCAC TthHB8I 792 TCGA T^CGA 1790 TCGA XcmI 17 CCAGCTACCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1297 CCAAGTCCTCGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AciI AcsI AfaI AflII AluI AlwI ApoI BanII BbvI BcnI BfaI BfrI BmyI BsaJI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I Cac8I CfoI Cfr13I DdeI DpnII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MwoI NciI NdeII NlaIII NlaIV PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI Tru9I TseI Tsp509I TspRI