Restriction map for pF1KB5691
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# NO CUT ENZYMES (  121 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AcyI        
AflII       AhdI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AviII       AvrII       BamHI       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BclI        BfrI        BglI        BlnI        BpuAI       
BsaBI       BsaHI       BsaI        BseAI       BseRI       
BsiEI       BsiWI       BsmBI       BspDI       BspEI       
BspLU11I    BsrBI       BssHII      Bst1107I    BstBI       
BstUI       BstZ17I     Bsu36I      CfoI        ClaI        
CpoI        DraI        DraIII      DrdI        DsaI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoRI       EcoRV       EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HhaI        Hin1I       HinP1I      HindIII     HpaI        
KasI        KpnI        KspI        MamI        MfeI        
MluI        MroI        MunI        MvnI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PaeR7I      PmaCI       PmeI        PmlI        PshAI       
PshBI       PvuI        RsrII       SacII       SbfI        
ScaI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    StuI        SwaI        XhoI        XmaCI       
XmaI        


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# ONE CUT ENZYMES (   60 enzymes) #
###################################

AccI          569  GTCGAC               GT^[AC][GT]AC
AclI          370  AACGTT               AA^CGTT
AcsI          106  GAATTT               [GA]^AATT[CT]
AflIII        412  ACGTGT               A^C[GA][CT]GT
AgeI         2519  ACCGGT               A^CCGGT
ApoI          106  GAATTT               [GA]^AATT[CT]
Asp700I      1216  GAACAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI        1575  GAGCTC               G[AT]GC[AT]^C
AspI         1978  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         2220  TGGCCA               TGG^CCA
BanI          275  GGCACC               G^G[CT][GA]CC
BcnI           74  CCCGG                CC^[GC]GG
BsaAI         411  TACGTG               [CT]AC^GT[GA]
BsaWI        2519  ACCGGT               [AT]^CCGG[AT]
BsgI          528  CTGCAC               GTGCAG(16/14)
BsiHKAI      1575  GAGCTC               G[AT]GC[AT]^C
BsmI          665  GAATGC               GAATGC(1/-1)
Bsp1407I      313  TGTACA               T^GTACA
BspHI        1920  TCATGA               T^CATGA
BspMI         697  GCAGGT               ACCTGC(4/8)
BsrFI        2519  ACCGGT               [GA]^CCGG[CT]
BsrGI         313  TGTACA               T^GTACA
BssSI         860  CACGAG               CACGAG(-5/-1)
BstAPI       2256  GCATTTGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       2363  GGTCACC              G^GT[ACGT]ACC
BstPI        2363  GGTCACC              G^GT[ACGT]ACC
BstXI        1721  CCAAGCCATTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I        2297  GCCTGC               GC[ACGT]^[ACGT]GC
Cfr10I       2519  ACCGGT               [GA]^CCGG[CT]
EaeI         2220  TGGCCA               [CT]^GGCC[GA]
EcoO65I      2363  GGTCACC              G^GT[ACGT]ACC
EcoT14I      1814  CCAAGG               C^C[AT][AT]GG
HgaI         2027  GACGC                GACGC(5/10)
HincII        569  GTCGAC               GT[CT]^[GA]AC
HindII        569  GTCGAC               GT[CT]^[GA]AC
MluNI        2220  TGGCCA               TGG^CCA
MscI         2220  TGGCCA               TGG^CCA
MslI          770  CATCTCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I       1211  CAGCTG               C[AC]G^C[GT]G
MwoI         2256  GCATTTGATGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI           74  CCCGG                CC^[GC]GG
PacI          559  TTAATTAA             TTAAT^TAA
PflFI        1978  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        2177  CCAATTTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI        2519  ACCGGT               A^CCGGT
Psp1406I      370  AACGTT               AA^CGTT
PvuII        1211  CAGCTG               CAG^CTG
RcaI         1920  TCATGA               T^CATGA
SacI         1575  GAGCTC               GAGCT^C
SalI          569  GTCGAC               G^TCGAC
SapI         1948  GCTCTTC              GCTCTTC(1/4)
SexAI        2016  ACCTGGT              A^CC[AT]GGT
SspBI         313  TGTACA               T^GTACA
SspI          357  AATATT               AAT^ATT
StyI         1814  CCAAGG               C^C[AT][AT]GG
Tth111I      1978  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       2177  CCAATTTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         1516  TCTAGA               T^CTAGA
XcmI           22  CCACACCTGTTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI         1216  GAACAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AlwI         1989  GATCC                GGATC(4/5)
             2238  GGATC               
AlwNI         950  CAGATACTG            CAG[ACGT][ACGT][ACGT]^CTG
             2012  CAGGACCTG           
AvaI            5  CTCGGG               C^[CT]CG[GA]G
               55  CTCGGG              
BanII        1398  GAGCCC               G[GA]GC[CT]^C
             1575  GAGCTC              
BciVI        2097  GGATAC               GTATCC(6/5)
              334  GTATCC              
BglII        1102  AGATCT               A^GATCT
             2357  AGATCT              
BlpI         1395  GCTGAGC              GC^T[ACGT]AGC
             2251  GCTGAGC             
Bpu1102I     1395  GCTGAGC              GC^T[ACGT]AGC
             2251  GCTGAGC             
BsoBI           5  CTCGGG               C^[CT]CG[GA]G
               55  CTCGGG              
BsrDI        1534  CATTGC               GCAATG(2/0)
             1192  GCAATG              
BstYI        1102  AGATCT               [GA]^GATC[CT]
             2357  AGATCT              
CelII        1395  GCTGAGC              GC^T[ACGT]AGC
             2251  GCTGAGC             
MflI         1102  AGATCT               [GA]^GATC[CT]
             2357  AGATCT              
XhoII        1102  AGATCT               [GA]^GATC[CT]
             2357  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   63 enzymes) #
#########################################

AciI        AfaI        AluI        AvaII       BbvI        
BfaI        BmyI        BpmI        BsaJI       BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       Cfr13I      DdeI        
DpnII       DraII       EarI        EcoO109I    EcoRII      
Fnu4HI      FokI        HaeIII      HapII       HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MnlI        
MseI        MspI        MvaI        NdeII       NlaIII      
NlaIV       PleI        PpuMI       PstI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     
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