Restriction map for pF1KB5676
##################################
# NO CUT ENZYMES (  108 enzymes) #
##################################

AatII       Acc65I      AclI        AcyI        AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       BamHI       BanII       BbeI        
BbrPI       BbvCI       BcgI        BcnI        BglI        
BglII       BlpI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsgI        BsiEI       BsiWI       Bsp1407I    
BspDI       BsrGI       BstAPI      BstBI       Bsu36I      
CelII       ClaI        CpoI        DraI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoRV       EcoT22I     FseI        Hin1I       HindIII     
HpaI        KasI        KpnI        KspI        MamI        
MluI        MspA1I      NaeI        NarI        NciI        
NdeI        NgoMIV      NotI        NruI        NsiI        
NspV        PacI        PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        PvuII       RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        Van91I      XbaI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccII        2228  CGCG                 CG^CG
AccIII        437  TCCGGA               T^CCGGA
AflII         403  CTTAAG               C^TTAAG
AflIII       1017  ACATGT               A^C[GA][CT]GT
AspI         1792  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          764  CTCGAG               C^[CT]CG[GA]G
AviII        1982  TGCGCA               TGC^GCA
AvrII         285  CCTAGG               C^CTAGG
BanI         1410  GGTGCC               G^G[CT][GA]CC
BfrI          403  CTTAAG               C^TTAAG
BlnI          285  CCTAGG               C^CTAGG
BsaI         1303  GAGACC               GGTCTC(1/5)
BseAI         437  TCCGGA               T^CCGGA
BseRI         871  GAGGAG               GAGGAG(10/8)
BsmBI         189  GAGACG               CGTCTC(1/5)
BsoBI         764  CTCGAG               C^[CT]CG[GA]G
BspEI         437  TCCGGA               T^CCGGA
BspLU11I     1017  ACATGT               A^CATGT
BspMI        1979  ACCTGC               ACCTGC(4/8)
BsrBI         961  GAGCGG               CCGCTC(-3/-3)
BsrDI        1590  CATTGC               GCAATG(2/0)
BsrFI        1871  ACCGGC               [GA]^CCGG[CT]
BssHII       2227  GCGCGC               G^CGCGC
BssSI        1754  CTCGTG               CACGAG(-5/-1)
BstEII       1419  GGTAACC              G^GT[ACGT]ACC
BstPI        1419  GGTAACC              G^GT[ACGT]ACC
BstUI        2228  CGCG                 CG^CG
BstXI        2000  CCAAGGATGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I       1871  ACCGGC               [GA]^CCGG[CT]
DraIII       1644  CACCATGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1904  GACCTGGCCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I      1419  GGTAACC              G^GT[ACGT]ACC
EcoRI        1109  GAATTC               G^AATTC
FspI         1982  TGCGCA               TGC^GCA
HaeII        1861  GGCGCT               [GA]GCGC^[CT]
HgaI         1127  GACGC                GACGC(5/10)
HincII        984  GTTGAC               GT[CT]^[GA]AC
HindII        984  GTTGAC               GT[CT]^[GA]AC
MfeI          695  CAATTG               C^AATTG
MroI          437  TCCGGA               T^CCGGA
MunI          695  CAATTG               C^AATTG
MvnI         2228  CGCG                 CG^CG
NcoI         2156  CCATGG               C^CATGG
NheI          636  GCTAGC               G^CTAGC
PaeR7I        764  CTCGAG               C^TCGAG
PflFI        1792  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI        1629  GGGTCCT              [GA]G^G[AT]CC[CT]
PstI          624  CTGCAG               CTGCA^G
Tth111I      1792  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI          764  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AccI          397  GTATAC               GT^[AC][GT]AC
             2383  GTATAC              
AcsI         1109  GAATTC               [GA]^AATT[CT]
             1504  AAATTC              
ApoI         1109  GAATTC               [GA]^AATT[CT]
             1504  AAATTC              
AspHI        1242  GTGCTC               G[AT]GC[AT]^C
             1492  GAGCAC              
BalI         1580  TGGCCA               TGG^CCA
             2090  TGGCCA              
BbsI         1429  GAAGAC               GAAGAC(2/6)
             2392  GAAGAC              
BciVI         338  GTATCC               GTATCC(6/5)
             1482  GTATCC              
BclI          803  TGATCA               T^GATCA
             1917  TGATCA              
BpuAI        1429  GAAGAC               GAAGAC(2/6)
             2392  GAAGAC              
BsaWI         437  TCCGGA               [AT]^CCGG[AT]
             1355  TCCGGT              
BsiHKAI      1242  GTGCTC               G[AT]GC[AT]^C
             1492  GAGCAC              
BsmI          885  GAATGC               GAATGC(1/-1)
             1600  GAATGC              
BspHI         821  TCATGA               T^CATGA
             1322  TCATGA              
Bst1107I      397  GTATAC               GTA^TAC
             2383  GTATAC              
BstZ17I       397  GTATAC               GTA^TAC
             2383  GTATAC              
DraII        1439  GGGGCCT              [GA]G^G[ACGT]CC[CT]
             1629  GGGTCCT             
EcoO109I     1439  GGGGCCT              [GA]G^G[ACGT]CC[CT]
             1629  GGGTCCT             
FauI         1697  CCCGC                CCCGC(4/6)
             1392  GCGGG               
FbaI          803  TGATCA               T^GATCA
             1917  TGATCA              
MaeII         336  ACGT                 A^CGT
             1458  ACGT                
MluNI        1580  TGGCCA               TGG^CCA
             2090  TGGCCA              
MscI         1580  TGGCCA               TGG^CCA
             2090  TGGCCA              
MslI         1210  CACGGGCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1216  CATGTGGGTG          
RcaI          821  TCATGA               T^CATGA
             1322  TCATGA              
Tsp45I        161  GTGAC                ^GT[GC]AC
             2243  GTGAC               
XcmI         1414  CCACAGGTAACCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2331  CCAGGGTGGAGCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   74 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AvaII       BbvI        BfaI        BmyI        BpmI        
BsaJI       BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DsaI        EaeI        EarI        EcoNI       
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MspI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       NspI        PleI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp509I     TspRI       TthHB8I     XhoII       
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