Restriction map for pF1KB5676
 (Linear) (Six-base) MAPSORT of: KIBB5676.seq  Check: 3664  from: 1  to: 2418



KIBB5676.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:28  ..

AccI GT'mk_AC

Cuts at:      0     398    2384    2418 
   Size:        398    1986      34

  Fragments arranged by size: 

                1986     398      34
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1126    1531    2269    2295    2306    2418 
   Size:       1126     405     738      26      11     112

  Fragments arranged by size: 

                1126     738     405     112      26      11
 
AflII C'TTAA_G

Cuts at:      0     403    2418 
   Size:        403    2015
 
AflIII A'CryG_T

Cuts at:      0    1017    2418 
   Size:       1017    1401
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1238    1257    2418 
   Size:       1238      19    1161

  Fragments arranged by size: 

                1238    1161      19
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2002    2034    2418 
   Size:       2002      32     384

  Fragments arranged by size: 

                2002     384      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1252    1473    1812    2185    2418 
   Size:       1252     221     339     373     233

  Fragments arranged by size: 

                1252     373     339     233     221
 
ApoI r'AATT_y

Cuts at:      0    1109    1504    2418 
   Size:       1109     395     914

  Fragments arranged by size: 

                1109     914     395
 
AvaI C'yCGr_G

Cuts at:      0     764    2418 
   Size:        764    1654
 
AvrII C'CTAG_G

Cuts at:      0     285    2418 
   Size:        285    2133
 
BanI G'GyrC_C

Cuts at:      0    1410    2418 
   Size:       1410    1008
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1436    2399    2418 
   Size:       1436     963      19

  Fragments arranged by size: 

                1436     963      19
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     349    1493    2418 
   Size:        349    1144     925

  Fragments arranged by size: 

                1144     925     349
 
BclI T'GATC_A

Cuts at:      0     803    1917    2418 
   Size:        803    1114     501

  Fragments arranged by size: 

                1114     803     501
 
Bme1580I G_kGCm'C

Cuts at:      0    1017    2418 
   Size:       1017    1401
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1987    2342    2418 
   Size:       1987     355      76

  Fragments arranged by size: 

                1987     355      76
 
BmtI G_CTAG'C

Cuts at:      0     640    2418 
   Size:        640    1778
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      29     515     806    2069    2418 
   Size:         29     486     291    1263     349

  Fragments arranged by size: 

                1263     486     349     291      29
 
Bpu10I CC'TnA_GC

Cuts at:      0    1634    2418 
   Size:       1634     784
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1386    1954    2418 
   Size:       1386     568     464

  Fragments arranged by size: 

                1386     568     464
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1297    2418 
   Size:       1297    1121
 
BsaWI w'CCGG_w

Cuts at:      0     437    1355    2418 
   Size:        437     918    1063

  Fragments arranged by size: 

                1063     918     437
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1603    1633    2418 
   Size:       1603      30     785

  Fragments arranged by size: 

                1603     785      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     886    2418 
   Size:        886    1532
 
BseYI C'CCAG_C

Cuts at:      0     125    1062    2418 
   Size:        125     937    1356

  Fragments arranged by size: 

                1356     937     125
 
BsiHKAI G_wGCw'C

Cuts at:      0    1246    1496    2418 
   Size:       1246     250     922

  Fragments arranged by size: 

                1246     922     250
 
BsmI GAATG_Cn'

Cuts at:      0     891    1606    2418 
   Size:        891     715     812

  Fragments arranged by size: 

                 891     812     715
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     183    2418 
   Size:        183    2235
 
Bsp1286I G_dGCh'C

Cuts at:      0    1017    1246    1496    2418 
   Size:       1017     229     250     922

  Fragments arranged by size: 

                1017     922     250     229
 
BspEI T'CCGG_A

Cuts at:      0     437    2418 
   Size:        437    1981
 
BspHI T'CATG_A

Cuts at:      0     821    1322    2418 
   Size:        821     501    1096

  Fragments arranged by size: 

                1096     821     501
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1988    2418 
   Size:       1988     430
 
BsrBI CCG'CTC

Cuts at:      0     963    2418 
   Size:        963    1455
 
BsrDI GCAATG_nn'

Cuts at:      0    1589    2418 
   Size:       1589     829
 
BsrFI r'CCGG_y

Cuts at:      0    1871    2418 
   Size:       1871     547
 
BssHII G'CGCG_C

Cuts at:      0    2227    2418 
   Size:       2227     191
 
BssSI C'ACGA_G

Cuts at:      0    1754    2418 
   Size:       1754     664
 
BstEII G'GTnAC_C

Cuts at:      0    1419    2418 
   Size:       1419     999
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2007    2418 
   Size:       2007     411
 
BstYI r'GATC_y

Cuts at:      0     504    1145    1281    1808    2189    2418 
   Size:        504     641     136     527     381     229

  Fragments arranged by size: 

                 641     527     504     381     229     136
 
BstZ17I GTA'TAC

Cuts at:      0     399    2385    2418 
   Size:        399    1986      33

  Fragments arranged by size: 

                1986     399      33
 
BtgI C'CryG_G

Cuts at:      0     462    1400    2156    2418 
   Size:        462     938     756     262

  Fragments arranged by size: 

                 938     756     462     262
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     909    2418 
   Size:        909    1509
 
BtsI GCAGTG_nn'

Cuts at:      0     546    1387    2364    2385    2418 
   Size:        546     841     977      21      33

  Fragments arranged by size: 

                 977     841     546      33      21
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1737    1772    2418 
   Size:       1737      35     646

  Fragments arranged by size: 

                1737     646      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    1649    2418 
   Size:       1649     769
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1910    2418 
   Size:       1910     508
 
EaeI y'GGCC_r

Cuts at:      0    1580    1908    2090    2418 
   Size:       1580     328     182     328

  Fragments arranged by size: 

                1580     328     328     182
 
EarI CTCTTCn'nnn_

Cuts at:      0     181     394     578    2162    2418 
   Size:        181     213     184    1584     256

  Fragments arranged by size: 

                1584     256     213     184     181
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      29     515     806    1126    1531    2069    2269    2295 
   Size:         29     486     291     320     405     538     200      26

Cuts at:   2295    2306    2418 
   Size:         11     112

  Fragments arranged by size: 

                 538     486     405     320     291     200     112      29
                  26      11
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     688     713     743    1189    2418 
   Size:        688      25      30     446    1229

  Fragments arranged by size: 

                1229     688     446      30      25
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1440    1630    2418 
   Size:       1440     190     788

  Fragments arranged by size: 

                1440     788     190
 
EcoRI G'AATT_C

Cuts at:      0    1109    2418 
   Size:       1109    1309
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     429    2418 
   Size:        429    1989
 
FspI TGC'GCA

Cuts at:      0    1984    2418 
   Size:       1984     434
 
HaeII r_GCGC'y

Cuts at:      0    1865    2418 
   Size:       1865     553
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      19      51     571     603     795     827    1816    1848 
   Size:         19      32     520      32     192      32     989      32

Cuts at:   1848    1936    1968    2418 
   Size:         88      32     450

  Fragments arranged by size: 

                 989     520     450     192      88      32      32      32
                  32      32      19
 
HincII GTy'rAC

Cuts at:      0     986    2418 
   Size:        986    1432
 
MfeI C'AATT_G

Cuts at:      0     695    2418 
   Size:        695    1723
 
MscI TGG'CCA

Cuts at:      0    1582    2092    2418 
   Size:       1582     510     326

  Fragments arranged by size: 

                1582     510     326
 
MslI CAynn'nnrTG

Cuts at:      0    1214    1220    2418 
   Size:       1214       6    1198

  Fragments arranged by size: 

                1214    1198       6
 
NcoI C'CATG_G

Cuts at:      0    2156    2418 
   Size:       2156     262
 
NheI G'CTAG_C

Cuts at:      0     636    2418 
   Size:        636    1782
 
NspI r_CATG'y

Cuts at:      0    1021    1219    1833    2418 
   Size:       1021     198     614     585

  Fragments arranged by size: 

                1021     614     585     198
 
PciI A'CATG_T

Cuts at:      0    1017    2418 
   Size:       1017    1401
 
PfoI T'CCnGG_A

Cuts at:      0     416     738    2418 
   Size:        416     322    1680

  Fragments arranged by size: 

                1680     416     322
 
PpuMI rG'GwC_Cy

Cuts at:      0    1630    2418 
   Size:       1630     788
 
PspXI vC'TCGA_Gb

Cuts at:      0     764    2418 
   Size:        764    1654
 
PstI C_TGCA'G

Cuts at:      0     628    2418 
   Size:        628    1790
 
SfcI C'TryA_G

Cuts at:      0     624     770    1121    1465    2418 
   Size:        624     146     351     344     953

  Fragments arranged by size: 

                 953     624     351     344     146
 
SmlI C'TyrA_G

Cuts at:      0     403     764    1365    1969    2418 
   Size:        403     361     601     604     449

  Fragments arranged by size: 

                 604     601     449     403     361
 
StyI C'CwwG_G

Cuts at:      0     285    1532    1748    2000    2156    2418 
   Size:        285    1247     216     252     156     262

  Fragments arranged by size: 

                1247     285     262     252     216     156
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1210    1511    1808    2418 
   Size:       1210     301     297     610

  Fragments arranged by size: 

                1210     610     301     297
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2319    2351    2418 
   Size:       2319      32      67

  Fragments arranged by size: 

                2319      67      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1795    2418 
   Size:       1795     623
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1421    2338    2418 
   Size:       1421     917      80

  Fragments arranged by size: 

                1421     917      80
 
XhoI C'TCGA_G

Cuts at:      0     764    2418 
   Size:        764    1654
 

 Enzymes that do cut:

     AccI     AcuI    AflII   AflIII     AlfI     AloI    AlwNI     ApoI
     AvaI    AvrII     BanI     BbsI    BciVI     BclI Bme1580I     BmrI
     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaWI    BsaXI    BseRI
    BseYI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstEII    BstXI    BstYI
  BstZ17I     BtgI    BtgZI     BtsI    CspCI   DraIII     DrdI     EaeI
     EarI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII     MfeI     MscI     MslI     NcoI     NheI     NspI
     PciI     PfoI    PpuMI    PspXI     PstI     SfcI     SmlI     StyI
    TaqII     TstI  Tth111I     XcmI     XhoI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI     AgeI     AhdI     AleI
     ApaI    ApaLI     AscI     AseI    AsiSI     BaeI    BamHI    BanII
     BbeI    BbvCI     BcgI    BfrBI     BglI    BglII     BlpI    BmgBI
     BplI    BsaAI    BsaBI    BsaHI     BsgI    BsiEI    BsiWI    BsrGI
   BstAPI    BstBI   Bsu36I     ClaI     DraI     EagI     EciI  EcoICRI
    EcoRV     FseI    FspAI  HindIII     HpaI     KasI     KpnI     MluI
     MmeI   MspA1I     NaeI     NarI     NdeI   NgoMIV     NotI     NruI
     NsiI     PacI     PasI    PflMI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI     PsrI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     TatI     XbaI     XmaI     XmnI     ZraI
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