Restriction map for pF1KB5663
##################################
# NO CUT ENZYMES (  154 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AviII       AvrII       BamHI       BanI        BbeI        
BbrPI       BbvCI       BcgI        BciVI       BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsaWI       
BseAI       BsiHKAI     BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrFI       BsrGI       BsrI        BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstXI       BstYI       BstZ17I     CelII       Cfr10I      
ClaI        CpoI        DraI        DraIII      DrdI        
Eam1105I    Eco47III    EcoO65I     EcoRI       EcoRV       
EcoT22I     FseI        FspI        HaeII       HgaI        
Hin1I       HincII      HindII      HindIII     HpaI        
KasI        KpnI        KspI        MaeI        MamI        
MfeI        MflI        MluI        MroI        MseI        
MunI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        TfiI        Tru9I       
Tsp509I     Tth111I     Van91I      XbaI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AfaI           48  GTAC                 GT^AC
AocI          648  CCTGAGG              CC^T[ACGT]AGG
AvaI          400  CCCGAG               C^[CT]CG[GA]G
BalI          239  TGGCCA               TGG^CCA
BbsI          229  GTCTTC               GAAGAC(2/6)
BclI          707  TGATCA               T^GATCA
BpuAI         229  GTCTTC               GAAGAC(2/6)
BsgI          142  GTGCAG               GTGCAG(16/14)
BsmFI         872  GGGAC                GGGAC(10/14)
BsoBI         400  CCCGAG               C^[CT]CG[GA]G
BspMI         109  ACCTGC               ACCTGC(4/8)
BsrDI         460  GCAATG               GCAATG(2/0)
BssHII        113  GCGCGC               G^CGCGC
Bsu36I        648  CCTGAGG              CC^T[ACGT]AGG
DraII         152  AGGTCCT              [GA]G^G[ACGT]CC[CT]
DsaI          602  CCATGG               C^C[GA][CT]GG
EagI          359  CGGCCG               C^GGCCG
EarI          823  GAAGAG               CTCTTC(1/4)
EclXI         359  CGGCCG               C^GGCCG
Eco52I        359  CGGCCG               C^GGCCG
Eco81I        648  CCTGAGG              CC^T[ACGT]AGG
EcoNI         774  CCTGCGCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      152  AGGTCCT              [GA]G^G[ACGT]CC[CT]
FbaI          707  TGATCA               T^GATCA
HinfI         766  GAGTC                G^A[ACGT]TC
HphI          432  GGTGA                GGTGA(8/7)
Ksp632I       823  GAAGAG               CTCTTC(1/4)
MaeIII        716  GTGAC                ^GT[ACGT]AC
MluNI         239  TGGCCA               TGG^CCA
MscI          239  TGGCCA               TGG^CCA
NcoI          602  CCATGG               C^CATGG
NspI          317  GCATGT               [GA]CATG^[CT]
PleI          766  GAGTC                GAGTC(4/5)
PpuMI         152  AGGTCCT              [GA]G^G[AT]CC[CT]
PstI          295  CTGCAG               CTGCA^G
PvuII         298  CAGCTG               CAG^CTG
RsaI           48  GTAC                 GT^AC
SfcI          295  CTGCAG               C^T[GA][CT]AG
SmlI          442  CTCAAG               C^T[CT][GA]AG
StuI          639  AGGCCT               AGG^CCT
TaqI          752  TCGA                 T^CGA
Tsp45I        716  GTGAC                ^GT[GC]AC
TspRI         632  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       752  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AlwNI          10  CAGCTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              298  CAGCTGCTG           
BanII         274  GAGCCC               G[GA]GC[CT]^C
              654  GAGCCC              
BcnI           75  CCGGG                CC^[GC]GG
              732  CCGGG               
BmyI          274  GAGCCC               G[AGT]GC[ACT]^C
              654  GAGCCC              
BsaI          106  GAGACC               GGTCTC(1/5)
              343  GAGACC              
BsiEI          72  CGACCG               CG[GA][CT]^CG
              359  CGGCCG              
BsmAI         106  GAGAC                GTCTC(1/5)
              343  GAGAC               
Bsp1286I      274  GAGCCC               G[AGT]GC[ACT]^C
              654  GAGCCC              
DdeI          332  CTAAG                C^T[ACGT]AG
              649  CTGAG               
EcoT14I       602  CCATGG               C^C[AT][AT]GG
              803  CCAAGG              
MaeII         866  ACGT                 A^CGT
              875  ACGT                
NciI           75  CCGGG                CC^[GC]GG
              732  CCGGG               
StyI          602  CCATGG               C^C[AT][AT]GG
              803  CCAAGG              
XcmI          182  CCACCTGGATGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              242  CCATGAACTACCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   46 enzymes) #
#########################################

AccII       AciI        AluI        AvaII       BbvI        
BpmI        BsaJI       BseRI       BsiYI       BslI        
BssKI       BstF5I      BstNI       BstUI       Cac8I       
CfoI        Cfr13I      DpnII       EaeI        EcoRII      
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HpaII       ItaI        MboI        
MboII       MnlI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NdeII       NlaIII      
NlaIV       Sau3AI      Sau96I      ScrFI       SfaNI       
TseI        
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