Restriction map for pF1KB5662
##################################
# NO CUT ENZYMES (  119 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AviII       AvrII       BalI        BamHI       BbrPI       
BcgI        BciVI       BclI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BsaBI       BsaI        
BsiEI       BsiWI       Bsp1407I    BspDI       BspHI       
BspLU11I    BsrDI       BsrGI       BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstZ17I     
Bsu36I      CelII       ClaI        DraI        DraIII      
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HincII      
HindII      HpaI        KpnI        MamI        MfeI        
MluI        MluNI       MscI        MunI        NcoI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        SacI        
SalI        SbfI        ScaI        SexAI       SfiI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        TfiI        
Tth111I     Van91I      XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccIII        781  TCCGGA               T^CCGGA
AcsI          884  AAATTC               [GA]^AATT[CT]
ApaI          259  GGGCCC               GGGCC^C
ApoI          884  AAATTC               [GA]^AATT[CT]
BbeI           34  GGCGCC               GGCGC^C
BbvCI         177  CCTCAGC              CCTCAGC(-5/-2)
BglI          182  GCCCCCCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI          159  CTCCAG               CTGGAG(16/14)
BsaAI         742  TACGTG               [CT]AC^GT[GA]
BseAI         781  TCCGGA               T^CCGGA
BseRI         646  GAGGAG               GAGGAG(10/8)
BsgI         1000  CTGCAC               GTGCAG(16/14)
BsmBI         957  GAGACG               CGTCTC(1/5)
BsmI          905  GCATTC               GAATGC(1/-1)
BspEI         781  TCCGGA               T^CCGGA
BspMI         281  ACCTGC               ACCTGC(4/8)
BsrFI         232  GCCGGC               [GA]^CCGG[CT]
BssHII        251  GCGCGC               G^CGCGC
BstXI         599  CCATCGTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I        232  GCCGGC               [GA]^CCGG[CT]
CpoI            9  CGGTCCG              CG^G[AT]CCG
DrdI          665  GACTAGAAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HindIII       619  AAGCTT               A^AGCTT
HphI          813  GGTGA                GGTGA(8/7)
KasI           34  GGCGCC               G^GCGCC
KspI           48  CCGCGG               CCGC^GG
MaeIII       1094  GTGAC                ^GT[ACGT]AC
MroI          781  TCCGGA               T^CCGGA
MseI          371  TTAA                 T^TAA
MslI          502  CACTCCTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI          232  GCCGGC               GCC^GGC
NarI           34  GGCGCC               GG^CGCC
NgoMIV        232  GCCGGC               G^CCGGC
PstI          198  CTGCAG               CTGCA^G
PvuII         406  CAGCTG               CAG^CTG
RsrII           9  CGGTCCG              CG^G[AT]CCG
SacII          48  CCGCGG               CCGC^GG
SfcI          198  CTGCAG               C^T[GA][CT]AG
SfoI           34  GGCGCC               GGC^GCC
SmlI          954  CTTGAG               C^T[CT][GA]AG
TaqI         1040  TCGA                 T^CGA
Tru9I         371  TTAA                 T^TAA
Tsp45I       1094  GTGAC                ^GT[GC]AC
Tsp509I       885  AATT                 ^AATT
TthHB8I      1040  TCGA                 T^CGA
XbaI          675  TCTAGA               T^CTAGA
XcmI           92  CCAAACGCCTGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AfaI         1035  GTAC                 GT^AC
             1080  GTAC                
AspHI         156  GTGCTC               G[AT]GC[AT]^C
              913  GAGCAC              
BanI           34  GGCGCC               G^G[CT][GA]CC
              935  GGTGCC              
BsaWI          12  TCCGGT               [AT]^CCGG[AT]
              781  TCCGGA              
BsiHKAI       156  GTGCTC               G[AT]GC[AT]^C
              913  GAGCAC              
BsrBI         221  CCGCTC               CCGCTC(-3/-3)
               30  GAGCGG              
BstF5I        330  CATCC                GGATG(2/0)
              402  CATCC               
BstYI         755  AGATCC               [GA]^GATC[CT]
              992  AGATCC              
DsaI           48  CCGCGG               C^C[GA][CT]GG
              641  CCACGG              
EaeI           89  CGGCCA               [CT]^GGCC[GA]
              131  CGGCCA              
EcoT14I       450  CCTTGG               C^C[AT][AT]GG
              461  CCAAGG              
FokI          330  CATCC                GGATG(9/13)
              402  CATCC               
HaeII          34  GGCGCC               [GA]GCGC^[CT]
              321  GGCGCT              
MaeII         743  ACGT                 A^CGT
              797  ACGT                
MflI          755  AGATCC               [GA]^GATC[CT]
              992  AGATCC              
MspA1I         48  CCGCGG               C[AC]G^C[GT]G
              406  CAGCTG              
NspI          309  GCATGT               [GA]CATG^[CT]
              497  ACATGC              
PpuMI         611  GGGACCT              [GA]G^G[AT]CC[CT]
              772  GGGACCT             
RsaI         1035  GTAC                 GT^AC
             1080  GTAC                
SapI           63  GCTCTTC              GCTCTTC(1/4)
              552  GCTCTTC             
SmaI          317  CCCGGG               CCC^GGG
             1030  CCCGGG              
StuI          394  AGGCCT               AGG^CCT
              464  AGGCCT              
StyI          450  CCTTGG               C^C[AT][AT]GG
              461  CCAAGG              
XhoII         755  AGATCC               [GA]^GATC[CT]
              992  AGATCC              
XmaCI         317  CCCGGG               C^CCGGG
             1030  CCCGGG              
XmaI          317  CCCGGG               C^CCGGG
             1030  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   66 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        AlwI        
AvaI        AvaII       BanII       BbsI        BbvI        
BcnI        BfaI        BmyI        BpuAI       BsaHI       
BsaJI       BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BsrI        BssKI       BstNI       
BstUI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       EarI        EcoO109I    EcoRII      
FauI        Fnu4HI      HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
ItaI        Ksp632I     MaeI        MboI        MboII       
MnlI        MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PleI        
Sau3AI      Sau96I      ScrFI       SfaNI       TseI        
TspRI       
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