Restriction map for pF1KB5662
 (Linear) (Six-base) MAPSORT of: KIBB5662.seq  Check: 9812  from: 1  to: 1116



KIBB5662.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:28  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     637    1043    1116 
   Size:        637     406      73

  Fragments arranged by size: 

                 637     406      73
 
ApaI G_GGCC'C

Cuts at:      0     263    1116 
   Size:        263     853
 
ApoI r'AATT_y

Cuts at:      0     884    1116 
   Size:        884     232
 
AvaI C'yCGr_G

Cuts at:      0      77     317     988    1030    1116 
   Size:         77     240     671      42      86

  Fragments arranged by size: 

                 671     240      86      77      42
 
BanI G'GyrC_C

Cuts at:      0      34     935    1116 
   Size:         34     901     181

  Fragments arranged by size: 

                 901     181      34
 
BanII G_rGCy'C

Cuts at:      0     263     751     968    1116 
   Size:        263     488     217     148

  Fragments arranged by size: 

                 488     263     217     148
 
BbeI G_GCGC'C

Cuts at:      0      38    1116 
   Size:         38    1078
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     465     627     686    1116 
   Size:        465     162      59     430

  Fragments arranged by size: 

                 465     430     162      59
 
BbvCI CC'TCA_GC

Cuts at:      0     178    1116 
   Size:        178     938
 
BglI GCCn_nnn'nGGC

Cuts at:      0     188    1116 
   Size:        188     928
 
Bme1580I G_kGCm'C

Cuts at:      0     142     263     873    1116 
   Size:        142     121     610     243

  Fragments arranged by size: 

                 610     243     142     121
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     779     860    1116 
   Size:        779      81     256

  Fragments arranged by size: 

                 779     256      81
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     144    1116 
   Size:        144     972
 
Bpu10I CC'TnA_GC

Cuts at:      0     178     622     911    1116 
   Size:        178     444     289     205

  Fragments arranged by size: 

                 444     289     205     178
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     975    1116 
   Size:        975     141
 
BsaAI yAC'GTr

Cuts at:      0     744    1116 
   Size:        744     372
 
BsaHI Gr'CG_yC

Cuts at:      0      35      83     107    1116 
   Size:         35      48      24    1009

  Fragments arranged by size: 

                1009      48      35      24
 
BsaWI w'CCGG_w

Cuts at:      0      12     781    1116 
   Size:         12     769     335

  Fragments arranged by size: 

                 769     335      12
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     487     517    1116 
   Size:        487      30     599

  Fragments arranged by size: 

                 599     487      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     661    1116 
   Size:        661     455
 
BseYI C'CCAG_C

Cuts at:      0      69     262     606     656     735    1116 
   Size:         69     193     344      50      79     381

  Fragments arranged by size: 

                 381     344     193      79      69      50
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     985    1116 
   Size:        985     131
 
BsiHKAI G_wGCw'C

Cuts at:      0     160     917    1116 
   Size:        160     757     199

  Fragments arranged by size: 

                 757     199     160
 
BsmI GAATG_Cn'

Cuts at:      0     905    1116 
   Size:        905     211
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     951    1116 
   Size:        951     165
 
Bsp1286I G_dGCh'C

Cuts at:      0     142     160     263     751     873     917     968    1116 
   Size:        142      18     103     488     122      44      51     148

  Fragments arranged by size: 

                 488     148     142     122     103      51      44      18
            
 
BspEI T'CCGG_A

Cuts at:      0     781    1116 
   Size:        781     335
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     290    1116 
   Size:        290     826
 
BsrBI CCG'CTC

Cuts at:      0      32     223    1116 
   Size:         32     191     893

  Fragments arranged by size: 

                 893     191      32
 
BsrFI r'CCGG_y

Cuts at:      0     232    1116 
   Size:        232     884
 
BssHII G'CGCG_C

Cuts at:      0     251    1116 
   Size:        251     865
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     606    1116 
   Size:        606     510
 
BstYI r'GATC_y

Cuts at:      0     755     992    1116 
   Size:        755     237     124

  Fragments arranged by size: 

                 755     237     124
 
BtgI C'CryG_G

Cuts at:      0      48     641    1116 
   Size:         48     593     475

  Fragments arranged by size: 

                 593     475      48
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     255     398    1116 
   Size:        255     143     718

  Fragments arranged by size: 

                 718     255     143
 
BtsI GCAGTG_nn'

Cuts at:      0     582     980    1116 
   Size:        582     398     136

  Fragments arranged by size: 

                 582     398     136
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     671    1116 
   Size:        671     445
 
EaeI y'GGCC_r

Cuts at:      0      89     131    1116 
   Size:         89      42     985

  Fragments arranged by size: 

                 985      89      42
 
EarI CTCTTCn'nnn_

Cuts at:      0      70     517     530     559    1116 
   Size:         70     447      13      29     557

  Fragments arranged by size: 

                 557     447      70      29      13
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     251     561    1116 
   Size:        251     310     555

  Fragments arranged by size: 

                 555     310     251
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     144     637    1043    1116 
   Size:        144     493     406      73

  Fragments arranged by size: 

                 493     406     144      73
 
EcoO109I rG'GnC_Cy

Cuts at:      0     259     612     773    1116 
   Size:        259     353     161     343

  Fragments arranged by size: 

                 353     343     259     161
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     466     651    1116 
   Size:        466     185     465

  Fragments arranged by size: 

                 466     465     185
 
HaeII r_GCGC'y

Cuts at:      0      38     325    1116 
   Size:         38     287     791

  Fragments arranged by size: 

                 791     287      38
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     298     330     487     519     700     732    1116 
   Size:        298      32     157      32     181      32     384

  Fragments arranged by size: 

                 384     298     181     157      32      32      32
 
HindIII A'AGCT_T

Cuts at:      0     619    1116 
   Size:        619     497
 
KasI G'GCGC_C

Cuts at:      0      34    1116 
   Size:         34    1082
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     816    1116 
   Size:        816     300
 
MslI CAynn'nnrTG

Cuts at:      0     506    1116 
   Size:        506     610
 
MspA1I CmG'CkG

Cuts at:      0      50     408    1116 
   Size:         50     358     708

  Fragments arranged by size: 

                 708     358      50
 
NaeI GCC'GGC

Cuts at:      0     234    1116 
   Size:        234     882
 
NarI GG'CG_CC

Cuts at:      0      35    1116 
   Size:         35    1081
 
NgoMIV G'CCGG_C

Cuts at:      0     232    1116 
   Size:        232     884
 
NspI r_CATG'y

Cuts at:      0     313     501    1116 
   Size:        313     188     615

  Fragments arranged by size: 

                 615     313     188
 
PfoI T'CCnGG_A

Cuts at:      0     244    1116 
   Size:        244     872
 
PpuMI rG'GwC_Cy

Cuts at:      0     612     773    1116 
   Size:        612     161     343

  Fragments arranged by size: 

                 612     343     161
 
PspOMI G'GGCC_C

Cuts at:      0     259    1116 
   Size:        259     857
 
PstI C_TGCA'G

Cuts at:      0     202    1116 
   Size:        202     914
 
PvuII CAG'CTG

Cuts at:      0     408    1116 
   Size:        408     708
 
RsrII CG'GwC_CG

Cuts at:      0      10    1116 
   Size:         10    1106
 
SacII CC_GC'GG

Cuts at:      0      51    1116 
   Size:         51    1065
 
SapI GCTCTTCn'nnn_

Cuts at:      0      70     559    1116 
   Size:         70     489     557

  Fragments arranged by size: 

                 557     489      70
 
SfcI C'TryA_G

Cuts at:      0     198    1116 
   Size:        198     918
 
SfoI GGC'GCC

Cuts at:      0      36    1116 
   Size:         36    1080
 
SmaI CCC'GGG

Cuts at:      0     319    1032    1116 
   Size:        319     713      84

  Fragments arranged by size: 

                 713     319      84
 
SmlI C'TyrA_G

Cuts at:      0     954    1116 
   Size:        954     162
 
StuI AGG'CCT

Cuts at:      0     396     466    1116 
   Size:        396      70     650

  Fragments arranged by size: 

                 650     396      70
 
StyI C'CwwG_G

Cuts at:      0     450     461    1116 
   Size:        450      11     655

  Fragments arranged by size: 

                 655     450      11
 
XbaI T'CTAG_A

Cuts at:      0     675    1116 
   Size:        675     441
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      99    1116 
   Size:         99    1017
 
XmaI C'CCGG_G

Cuts at:      0     317    1030    1116 
   Size:        317     713      86

  Fragments arranged by size: 

                 713     317      86
 

 Enzymes that do cut:

     AcuI     ApaI     ApoI     AvaI     BanI    BanII     BbeI     BbsI
    BbvCI     BglI Bme1580I     BmrI     BpmI   Bpu10I    BpuEI    BsaAI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrFI   BssHII    BstXI
    BstYI     BtgI    BtgZI     BtsI     DrdI     EaeI     EarI     EciI
  Eco57MI EcoO109I     FalI    HaeII    Hin4I  HindIII     KasI     MmeI
     MslI   MspA1I     NaeI     NarI   NgoMIV     NspI     PfoI    PpuMI
   PspOMI     PstI    PvuII    RsrII    SacII     SapI     SfcI     SfoI
     SmaI     SmlI     StuI     StyI     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BcgI    BciVI     BclI
    BfrBI    BglII     BlpI    BmgBI     BmtI     BplI     BsaI    BsaBI
    BsiEI    BsiWI    BspHI    BsrDI    BsrGI    BssSI   BstAPI    BstBI
   BstEII  BstZ17I   Bsu36I     ClaI    CspCI     DraI   DraIII     EagI
  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI   HincII
     HpaI     KpnI     MfeI     MluI     MscI     NcoI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PmeI
     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI     SacI
     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI    TaqII     TatI
     TstI  Tth111I     XhoI     XmnI     ZraI
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