Restriction map for pF1KB5642
##################################
# NO CUT ENZYMES (  143 enzymes) #
##################################

AccI        AccIII      AclI        AcsI        AflII       
AflIII      AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     AspEI       AspHI       
AspI        AviII       AvrII       BalI        BamHI       
BanII       BbrPI       BbvCI       BcgI        BciVI       
BclI        BfrI        BglII       BlnI        BlpI        
BmyI        Bpu1102I    BsaAI       BsaBI       BsaWI       
BseAI       BsiHKAI     BsiWI       BsmI        Bsp1286I    
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrGI       BssHII      Bst1107I    
BstBI       BstEII      BstNI       BstPI       BstXI       
BstYI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DdeI        DraI        DraIII      DrdI        
DsaI        Eam1105I    Eco47III    Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRII      EcoRV       EcoT22I     
FbaI        FseI        FspI        HincII      HindII      
HindIII     HpaI        KspI        MamI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MslI        MunI        MvaI        NcoI        NdeI        
NheI        NruI        NsiI        NspV        PacI        
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfcI        SfiI        SfuI        SnaBI       
SpeI        Sse8387I    SspBI       SspI        SwaI        
Tsp509I     TspRI       Tth111I     Van91I      XbaI        
XcmI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   30 enzymes) #
###################################

AatII         262  GACGTC               GACGT^C
Acc65I        551  GGTACC               G^GTACC
AlwI          750  GATCC                GGATC(4/5)
AlwNI         529  CAGTCGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp718I       551  GGTACC               G^GTACC
BglI          814  GCCTTTGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI          631  GAGACC               GGTCTC(1/5)
BsgI          658  GTGCAG               GTGCAG(16/14)
BsmBI         673  GAGACG               CGTCTC(1/5)
BssSI         670  CACGAG               CACGAG(-5/-1)
BstAPI       1006  GCAGGTGTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DpnII         750  GATC                 ^GATC
EarI          367  GAAGAG               CTCTTC(1/4)
KpnI          551  GGTACC               GGTAC^C
Ksp632I       367  GAAGAG               CTCTTC(1/4)
MboI          750  GATC                 ^GATC
MseI          920  TTAA                 T^TAA
NdeII         750  GATC                 ^GATC
NotI          231  GCGGCCGC             GC^GGCCGC
NspI          689  GCATGC               [GA]CATG^[CT]
PaeR7I        740  CTCGAG               C^TCGAG
Sau3AI        750  GATC                 ^GATC
SmaI           90  CCCGGG               CCC^GGG
SphI          689  GCATGC               GCATG^C
StuI          122  AGGCCT               AGG^CCT
TfiI          445  GAATC                G^A[AT]TC
Tru9I         920  TTAA                 T^TAA
XhoI          740  CTCGAG               C^TCGAG
XmaCI          90  CCCGGG               C^CCGGG
XmaI           90  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AvaII         255  GGTCC                G^G[AT]CC
              966  GGACC               
BbsI          718  GAAGAC               GAAGAC(2/6)
              834  GAAGAC              
BfaI          916  CTAG                 C^TAG
              970  CTAG                
BpuAI         718  GAAGAC               GAAGAC(2/6)
              834  GAAGAC              
BsmFI         908  GGGAC                GGGAC(10/14)
              256  GTCCC               
BspMI         599  GCAGGT               ACCTGC(4/8)
             1006  GCAGGT              
BstF5I        651  GGATG                GGATG(2/0)
              936  GGATG               
DraII         254  AGGTCCC              [GA]G^G[ACGT]CC[CT]
              965  AGGACCT             
EcoO109I      254  AGGTCCC              [GA]G^G[ACGT]CC[CT]
              965  AGGACCT             
EcoT14I       578  CCAAGG               C^C[AT][AT]GG
              684  CCAAGG              
FokI          651  GGATG                GGATG(9/13)
              936  GGATG               
HgaI          349  GACGC                GACGC(5/10)
              486  GACGC               
HphI          165  GGTGA                GGTGA(8/7)
              266  TCACC               
MaeI          916  CTAG                 C^TAG
              970  CTAG                
MaeII         263  ACGT                 A^CGT
              887  ACGT                
PpuMI         254  AGGTCCC              [GA]G^G[AT]CC[CT]
              965  AGGACCT             
SfaNI         857  GCATC                GCATC(5/9)
              980  GCATC               
SgrAI         180  CGCCGGCG             C[GA]^CCGG[CT]G
              570  CACCGGCG            
SmlI          322  CTTGAG               C^T[CT][GA]AG
              740  CTCGAG              
StyI          578  CCAAGG               C^C[AT][AT]GG
              684  CCAAGG              


#########################################
# 3 OR MORE CUT ENZYMES (   65 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AvaI        BanI        BbeI        BbvI        BcnI        
BpmI        BsaHI       BsaJI       BseRI       BsiEI       
BsiYI       BslI        BsmAI       BsoBI       BsrFI       
BsrI        BssKI       BstUI       Cac8I       CfoI        
Cfr10I      Cfr13I      EaeI        EagI        EclXI       
Eco52I      FauI        Fnu4HI      HaeII       HaeIII      
HapII       HhaI        Hin1I       HinP1I      HinfI       
HpaII       ItaI        KasI        MaeIII      MboII       
MnlI        MspA1I      MspI        MvnI        MwoI        
NaeI        NarI        NciI        NgoMIV      NlaIII      
NlaIV       PleI        RsaI        Sau96I      ScrFI       
SfoI        TaqI        TseI        Tsp45I      TthHB8I     
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