Restriction map for pF1KB5642
 (Linear) (Six-base) MAPSORT of: KIBB5642.seq  Check: 7829  from: 1  to: 1050



KIBB5642.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:28  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     997    1050 
   Size:        997      53
 
AatII G_ACGT'C

Cuts at:      0     266    1050 
   Size:        266     784
 
Acc65I G'GTAC_C

Cuts at:      0     551    1050 
   Size:        551     499
 
AlwNI CAG_nnn'CTG

Cuts at:      0     534    1050 
   Size:        534     516
 
AvaI C'yCGr_G

Cuts at:      0      90     400     740    1050 
   Size:         90     310     340     310

  Fragments arranged by size: 

                 340     310     310      90
 
BanI G'GyrC_C

Cuts at:      0      79     247     315     551     574     811    1050 
   Size:         79     168      68     236      23     237     239

  Fragments arranged by size: 

                 239     237     236     168      79      68      23
 
BbeI G_GCGC'C

Cuts at:      0      83     251     319     578    1050 
   Size:         83     168      68     259     472

  Fragments arranged by size: 

                 472     259     168      83      68
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     725     841    1050 
   Size:        725     116     209

  Fragments arranged by size: 

                 725     209     116
 
BglI GCCn_nnn'nGGC

Cuts at:      0     820    1050 
   Size:        820     230
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     789    1050 
   Size:        789     261
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     561    1050 
   Size:        561     489
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     451     556     649    1037    1050 
   Size:        451     105      93     388      13

  Fragments arranged by size: 

                 451     388     105      93      13
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     343    1050 
   Size:        343     707
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     625    1050 
   Size:        625     425
 
BsaHI Gr'CG_yC

Cuts at:      0      80     248     263     316     350     487     575    1050 
   Size:         80     168      15      53      34     137      88     475

  Fragments arranged by size: 

                 475     168     137      88      80      53      34      15
            
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     502     532    1050 
   Size:        502      30     518

  Fragments arranged by size: 

                 518     502      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     340     385     517     520     523    1050 
   Size:        340      45     132       3       3     527

  Fragments arranged by size: 

                 527     340     132      45       3       3
 
BseYI C'CCAG_C

Cuts at:      0     932    1050 
   Size:        932     118
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     679    1050 
   Size:        679     371
 
BsiEI CG_ry'CG

Cuts at:      0     235     344     412    1050 
   Size:        235     109      68     638

  Fragments arranged by size: 

                 638     235     109      68
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     667    1050 
   Size:        667     383
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     590     997    1050 
   Size:        590     407      53

  Fragments arranged by size: 

                 590     407      53
 
BsrFI r'CCGG_y

Cuts at:      0      55      67     157     181     411     524     571    1050 
   Size:         55      12      90      24     230     113      47     479

  Fragments arranged by size: 

                 479     230     113      90      55      47      24      12
            
 
BssSI C'ACGA_G

Cuts at:      0     670    1050 
   Size:        670     380
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1012    1050 
   Size:       1012      38
 
EaeI y'GGCC_r

Cuts at:      0     232     341     409    1050 
   Size:        232     109      68     641

  Fragments arranged by size: 

                 641     232     109      68
 
EagI C'GGCC_G

Cuts at:      0     232     341     409    1050 
   Size:        232     109      68     641

  Fragments arranged by size: 

                 641     232     109      68
 
EarI CTCTTCn'nnn_

Cuts at:      0     362    1050 
   Size:        362     688
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     188    1050 
   Size:        188     862
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     451     556     649    1037    1050 
   Size:        451     105      93     388      13

  Fragments arranged by size: 

                 451     388     105      93      13
 
EcoO109I rG'GnC_Cy

Cuts at:      0     255     966    1050 
   Size:        255     711      84

  Fragments arranged by size: 

                 711     255      84
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     709    1050 
   Size:        709     341
 
HaeII r_GCGC'y

Cuts at:      0      83     182     251     319     578     628    1050 
   Size:         83      99      69      68     259      50     422

  Fragments arranged by size: 

                 422     259      99      83      69      68      50
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      23      55     531     563     715     734     747     766 
   Size:         23      32     476      32     152      19      13      19

Cuts at:    766     780     812    1050 
   Size:         14      32     238

  Fragments arranged by size: 

                 476     238     152      32      32      32      23      19
                  19      14      13
 
KasI G'GCGC_C

Cuts at:      0      79     247     315     574    1050 
   Size:         79     168      68     259     476

  Fragments arranged by size: 

                 476     259     168      79      68
 
KpnI G_GTAC'C

Cuts at:      0     555    1050 
   Size:        555     495
 
MspA1I CmG'CkG

Cuts at:      0      74     346     426    1050 
   Size:         74     272      80     624

  Fragments arranged by size: 

                 624     272      80      74
 
NaeI GCC'GGC

Cuts at:      0      57      69     159     183     413    1050 
   Size:         57      12      90      24     230     637

  Fragments arranged by size: 

                 637     230      90      57      24      12
 
NarI GG'CG_CC

Cuts at:      0      80     248     316     575    1050 
   Size:         80     168      68     259     475

  Fragments arranged by size: 

                 475     259     168      80      68
 
NgoMIV G'CCGG_C

Cuts at:      0      55      67     157     181     411    1050 
   Size:         55      12      90      24     230     639

  Fragments arranged by size: 

                 639     230      90      55      24      12
 
NotI GC'GGCC_GC

Cuts at:      0     232    1050 
   Size:        232     818
 
NspI r_CATG'y

Cuts at:      0     693    1050 
   Size:        693     357
 
PfoI T'CCnGG_A

Cuts at:      0     217    1050 
   Size:        217     833
 
PpuMI rG'GwC_Cy

Cuts at:      0     255     966    1050 
   Size:        255     711      84

  Fragments arranged by size: 

                 711     255      84
 
SfoI GGC'GCC

Cuts at:      0      81     249     317     576    1050 
   Size:         81     168      68     259     474

  Fragments arranged by size: 

                 474     259     168      81      68
 
SgrAI Cr'CCGG_yG

Cuts at:      0     181     571    1050 
   Size:        181     390     479

  Fragments arranged by size: 

                 479     390     181
 
SmaI CCC'GGG

Cuts at:      0      92    1050 
   Size:         92     958
 
SmlI C'TyrA_G

Cuts at:      0     322     740    1050 
   Size:        322     418     310

  Fragments arranged by size: 

                 418     322     310
 
SphI G_CATG'C

Cuts at:      0     693    1050 
   Size:        693     357
 
StuI AGG'CCT

Cuts at:      0     124    1050 
   Size:        124     926
 
StyI C'CwwG_G

Cuts at:      0     578     684    1050 
   Size:        578     106     366

  Fragments arranged by size: 

                 578     366     106
 
XhoI C'TCGA_G

Cuts at:      0     740    1050 
   Size:        740     310
 
XmaI C'CCGG_G

Cuts at:      0      90    1050 
   Size:         90     960
 
ZraI GAC'GTC

Cuts at:      0     264    1050 
   Size:        264     786
 

 Enzymes that do cut:

     AarI    AatII   Acc65I    AlwNI     AvaI     BanI     BbeI     BbsI
     BglI     BmrI     BplI     BpmI    BpuEI     BsaI    BsaHI    BsaXI
    BseRI    BseYI     BsgI    BsiEI    BsmBI    BspMI    BsrFI    BssSI
   BstAPI     EaeI     EagI     EarI     EciI  Eco57MI EcoO109I     FalI
    HaeII    Hin4I     KasI     KpnI   MspA1I     NaeI     NarI   NgoMIV
     NotI     NspI     PfoI    PpuMI     SfoI    SgrAI     SmaI     SmlI
     SphI     StuI     StyI     XhoI     XmaI     ZraI

 Enzymes that do not cut: 

     AccI     AclI     AcuI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI    BanII    BbvCI     BcgI    BciVI
     BclI    BfrBI    BglII     BlpI Bme1580I    BmgBI     BmtI   Bpu10I
    BsaAI    BsaBI    BsaWI  BsiHKAI    BsiWI     BsmI Bsp1286I    BspEI
    BspHI    BsrBI    BsrDI    BsrGI   BssHII    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI
     FspI    FspAI   HincII  HindIII     HpaI     MfeI     MluI     MmeI
     MscI     MslI     NcoI     NdeI     NheI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SgfI    SnaBI     SpeI     SrfI     SspI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmnI
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