Restriction map for pF1KB5639
##################################
# NO CUT ENZYMES (  129 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AviII       AvrII       BalI        BamHI       
BbeI        BbrPI       BciVI       BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BsaWI       BseAI       BsiWI       
BsmI        BspDI       BspEI       BspMI       BsrDI       
BssHII      BssSI       Bst1107I    BstBI       BstYI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraI        DrdI        Eam1105I    EarI        Eco47III    
Eco81I      EcoNI       EcoRI       EcoRV       EcoT22I     
FseI        FspI        HaeII       Hin1I       HindIII     
HpaI        KasI        KpnI        Ksp632I     MaeII       
MamI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MseI        MunI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PstI        
PvuI        RsrII       SalI        SapI        SbfI        
ScaI        SfiI        SfoI        SfuI        SgrAI       
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspI        SwaI        Tru9I       Tsp509I     Van91I      
XcmI        XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AflIII        902  ACATGT               A^C[GA][CT]GT
AlwI          997  GATCC                GGATC(4/5)
ApaI           38  GGGCCC               GGGCC^C
AspI          805  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          913  CCCGGG               C^[CT]CG[GA]G
BbsI          265  GAAGAC               GAAGAC(2/6)
BcgI         1026  CGATGACTTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          164  TGATCA               T^GATCA
BpuAI         265  GAAGAC               GAAGAC(2/6)
BsiEI          26  CGGCCG               CG[GA][CT]^CG
BsoBI         913  CCCGGG               C^[CT]CG[GA]G
BspHI        1070  TCATGA               T^CATGA
BspLU11I      902  ACATGT               A^CATGT
BsrFI         939  ACCGGC               [GA]^CCGG[CT]
BsrI          317  ACTGG                ACTGG(1/-1)
BstEII        591  GGTGACC              G^GT[ACGT]ACC
BstPI         591  GGTGACC              G^GT[ACGT]ACC
BstXI         482  CCAGAGGGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I        939  ACCGGC               [GA]^CCGG[CT]
DsaI          128  CCGCGG               C^C[GA][CT]GG
EaeI           26  CGGCCG               [CT]^GGCC[GA]
EagI           26  CGGCCG               C^GGCCG
EclXI          26  CGGCCG               C^GGCCG
Eco52I         26  CGGCCG               C^GGCCG
EcoO65I       591  GGTGACC              G^GT[ACGT]ACC
EcoT14I      1214  CCTTGG               C^C[AT][AT]GG
FbaI          164  TGATCA               T^GATCA
HgaI         1221  GACGC                GACGC(5/10)
KspI          128  CCGCGG               CCGC^GG
MslI          316  CACTGGAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI          902  ACATGT               [GA]CATG^[CT]
PflFI         805  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
RcaI         1070  TCATGA               T^CATGA
SacI         1269  GAGCTC               GAGCT^C
SacII         128  CCGCGG               CCGC^GG
SexAI         349  ACCAGGT              A^CC[AT]GGT
SfcI          306  CTATAG               C^T[GA][CT]AG
SmaI          913  CCCGGG               CCC^GGG
StuI           85  AGGCCT               AGG^CCT
StyI         1214  CCTTGG               C^C[AT][AT]GG
Tth111I       805  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         1290  TCTAGA               T^CTAGA
XmaCI         913  CCCGGG               C^CCGGG
XmaI          913  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AccII          74  CGCG                 CG^CG
              129  CGCG                
AlwNI         944  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1304  CAGACTCTG           
AspHI         115  GTGCTC               G[AT]GC[AT]^C
             1269  GAGCTC              
BanI          424  GGCACC               G^G[CT][GA]CC
             1044  GGTGCC              
BbvCI         799  GCTGAGG              CCTCAGC(-5/-2)
             1117  GCTGAGG             
BfaI          181  CTAG                 C^TAG
             1291  CTAG                
BpmI          597  CTCCAG               CTGGAG(16/14)
              516  CTGGAG              
BsaI          144  GGTCTC               GGTCTC(1/5)
              956  GGTCTC              
BsgI          345  CTGCAC               GTGCAG(16/14)
              384  CTGCAC              
BsiHKAI       115  GTGCTC               G[AT]GC[AT]^C
             1269  GAGCTC              
BsmBI         586  GAGACG               CGTCTC(1/5)
             1219  GAGACG              
Bsp1407I      508  TGTACA               T^GTACA
             1145  TGTACA              
BsrBI        1066  CCGCTC               CCGCTC(-3/-3)
              197  GAGCGG              
BsrGI         508  TGTACA               T^GTACA
             1145  TGTACA              
BstAPI        281  GCATCTCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1303  GCAGACTCTGC         
BstF5I        488  GGATG                GGATG(2/0)
             1209  GGATG               
BstUI          74  CGCG                 CG^CG
              129  CGCG                
CfoI           73  GCGC                 GCG^C
              342  GCGC                
FauI           75  GCGGG                CCCGC(4/6)
             1126  GCGGG               
FokI          488  GGATG                GGATG(9/13)
             1209  GGATG               
HhaI           73  GCGC                 GCG^C
              342  GCGC                
HinP1I         73  GCGC                 G^CGC
              342  GCGC                
HincII        898  GTCAAC               GT[CT]^[GA]AC
             1168  GTCAAC              
HindII        898  GTCAAC               GT[CT]^[GA]AC
             1168  GTCAAC              
MaeI          181  CTAG                 C^TAG
             1291  CTAG                
MvnI           74  CGCG                 CG^CG
              129  CGCG                
PvuII         149  CAGCTG               CAG^CTG
              575  CAGCTG              
SspBI         508  TGTACA               T^GTACA
             1145  TGTACA              
TaqI          527  TCGA                 T^CGA
             1025  TCGA                
TthHB8I       527  TCGA                 T^CGA
             1025  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   55 enzymes) #
#########################################

AciI        AfaI        AluI        AvaII       BanII       
BbvI        BcnI        BmyI        BsaJI       BseRI       
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BssKI       BstNI       Cac8I       Cfr13I      DdeI        
DpnII       DraII       DraIII      EcoO109I    EcoRII      
Fnu4HI      HaeIII      HapII       HinfI       HpaII       
HphI        ItaI        MaeIII      MboI        MboII       
MnlI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PleI        
PpuMI       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       TfiI        TseI        Tsp45I      TspRI       
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