Restriction map for pF1KB5639
 (Linear) (Six-base) MAPSORT of: KIBB5639.seq  Check: 5758  from: 1  to: 1320



KIBB5639.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:28  ..

AflIII A'CryG_T

Cuts at:      0     902    1320 
   Size:        902     418
 
AlwNI CAG_nnn'CTG

Cuts at:      0     949    1309    1320 
   Size:        949     360      11

  Fragments arranged by size: 

                 949     360      11
 
ApaI G_GGCC'C

Cuts at:      0      42    1320 
   Size:         42    1278
 
AvaI C'yCGr_G

Cuts at:      0     913    1320 
   Size:        913     407
 
BanI G'GyrC_C

Cuts at:      0     424    1044    1320 
   Size:        424     620     276

  Fragments arranged by size: 

                 620     424     276
 
BanII G_rGCy'C

Cuts at:      0      42      95    1273    1320 
   Size:         42      53    1178      47

  Fragments arranged by size: 

                1178      53      47      42
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     272    1320 
   Size:        272    1048
 
BbvCI CC'TCA_GC

Cuts at:      0     800    1118    1320 
   Size:        800     318     202

  Fragments arranged by size: 

                 800     318     202
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1015    1049    1320 
   Size:       1015      34     271

  Fragments arranged by size: 

                1015     271      34
 
BclI T'GATC_A

Cuts at:      0     164    1320 
   Size:        164    1156
 
Bme1580I G_kGCm'C

Cuts at:      0      42    1049    1320 
   Size:         42    1007     271

  Fragments arranged by size: 

                1007     271      42
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     537     582    1320 
   Size:        537      45     738

  Fragments arranged by size: 

                 738     537      45
 
Bpu10I CC'TnA_GC

Cuts at:      0     744     764     800     854    1118    1320 
   Size:        744      20      36      54     264     202

  Fragments arranged by size: 

                 744     264     202      54      36      20
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     150     962    1320 
   Size:        150     812     358

  Fragments arranged by size: 

                 812     358     150
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     641     671    1320 
   Size:        641      30     649

  Fragments arranged by size: 

                 649     641      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     598     978     981    1320 
   Size:        598     380       3     339

  Fragments arranged by size: 

                 598     380     339       3
 
BseYI C'CCAG_C

Cuts at:      0     188     573    1224    1320 
   Size:        188     385     651      96

  Fragments arranged by size: 

                 651     385     188      96
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     330     369    1320 
   Size:        330      39     951

  Fragments arranged by size: 

                 951     330      39
 
BsiEI CG_ry'CG

Cuts at:      0      29    1320 
   Size:         29    1291
 
BsiHKAI G_wGCw'C

Cuts at:      0     119    1273    1320 
   Size:        119    1154      47

  Fragments arranged by size: 

                1154     119      47
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     580    1213    1320 
   Size:        580     633     107

  Fragments arranged by size: 

                 633     580     107
 
Bsp1286I G_dGCh'C

Cuts at:      0      42      95     119    1049    1273    1320 
   Size:         42      53      24     930     224      47

  Fragments arranged by size: 

                 930     224      53      47      42      24
 
BspHI T'CATG_A

Cuts at:      0    1070    1320 
   Size:       1070     250
 
BsrBI CCG'CTC

Cuts at:      0     199    1068    1320 
   Size:        199     869     252

  Fragments arranged by size: 

                 869     252     199
 
BsrFI r'CCGG_y

Cuts at:      0     939    1320 
   Size:        939     381
 
BsrGI T'GTAC_A

Cuts at:      0     508    1145    1320 
   Size:        508     637     175

  Fragments arranged by size: 

                 637     508     175
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     287    1309    1320 
   Size:        287    1022      11

  Fragments arranged by size: 

                1022     287      11
 
BstEII G'GTnAC_C

Cuts at:      0     591    1320 
   Size:        591     729
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     489    1320 
   Size:        489     831
 
BtgI C'CryG_G

Cuts at:      0     128    1320 
   Size:        128    1192
 
DraIII CAC_nnn'GTG

Cuts at:      0     353     407     476    1320 
   Size:        353      54      69     844

  Fragments arranged by size: 

                 844     353      69      54
 
EaeI y'GGCC_r

Cuts at:      0      26    1320 
   Size:         26    1294
 
EagI C'GGCC_G

Cuts at:      0      26    1320 
   Size:         26    1294
 
EcoICRI GAG'CTC

Cuts at:      0    1271    1320 
   Size:       1271      49
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     537     582    1320 
   Size:        537      45     738

  Fragments arranged by size: 

                 738     537      45
 
EcoO109I rG'GnC_Cy

Cuts at:      0      38      61      99     759     849    1320 
   Size:         38      23      38     660      90     471

  Fragments arranged by size: 

                 660     471      90      38      38      23
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     242     266     268     274     298     300     476     508 
   Size:        242      24       2       6      24       2     176      32

Cuts at:    508     766     798    1320 
   Size:        258      32     522

  Fragments arranged by size: 

                 522     258     242     176      32      32      24      24
                   6       2       2
 
HincII GTy'rAC

Cuts at:      0     900    1170    1320 
   Size:        900     270     150

  Fragments arranged by size: 

                 900     270     150
 
MslI CAynn'nnrTG

Cuts at:      0     320    1320 
   Size:        320    1000
 
MspA1I CmG'CkG

Cuts at:      0     130     151     577    1126    1320 
   Size:        130      21     426     549     194

  Fragments arranged by size: 

                 549     426     194     130      21
 
NspI r_CATG'y

Cuts at:      0     906    1320 
   Size:        906     414
 
PasI CC'CwG_GG

Cuts at:      0      65     604    1320 
   Size:         65     539     716

  Fragments arranged by size: 

                 716     539      65
 
PciI A'CATG_T

Cuts at:      0     902    1320 
   Size:        902     418
 
PfoI T'CCnGG_A

Cuts at:      0     431     872    1320 
   Size:        431     441     448

  Fragments arranged by size: 

                 448     441     431
 
PpuMI rG'GwC_Cy

Cuts at:      0      61      99     759     849    1320 
   Size:         61      38     660      90     471

  Fragments arranged by size: 

                 660     471      90      61      38
 
PspOMI G'GGCC_C

Cuts at:      0      38    1320 
   Size:         38    1282
 
PvuII CAG'CTG

Cuts at:      0     151     577    1320 
   Size:        151     426     743

  Fragments arranged by size: 

                 743     426     151
 
SacI G_AGCT'C

Cuts at:      0    1273    1320 
   Size:       1273      47
 
SacII CC_GC'GG

Cuts at:      0     131    1320 
   Size:        131    1189
 
SanDI GG'GwC_CC

Cuts at:      0      99     759    1320 
   Size:         99     660     561

  Fragments arranged by size: 

                 660     561      99
 
SexAI A'CCwGG_T

Cuts at:      0     349    1320 
   Size:        349     971
 
SfcI C'TryA_G

Cuts at:      0     306    1320 
   Size:        306    1014
 
SmaI CCC'GGG

Cuts at:      0     915    1320 
   Size:        915     405
 
StuI AGG'CCT

Cuts at:      0      87    1320 
   Size:         87    1233
 
StyI C'CwwG_G

Cuts at:      0    1214    1320 
   Size:       1214     106
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     184     859    1320 
   Size:        184     675     461

  Fragments arranged by size: 

                 675     461     184
 
TatI w'GTAC_w

Cuts at:      0     508    1145    1320 
   Size:        508     637     175

  Fragments arranged by size: 

                 637     508     175
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     462     494    1320 
   Size:        462      32     826

  Fragments arranged by size: 

                 826     462      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     808    1320 
   Size:        808     512
 
XbaI T'CTAG_A

Cuts at:      0    1290    1320 
   Size:       1290      30
 
XmaI C'CCGG_G

Cuts at:      0     913    1320 
   Size:        913     407
 

 Enzymes that do cut:

   AflIII    AlwNI     ApaI     AvaI     BanI    BanII     BbsI    BbvCI
     BcgI     BclI Bme1580I     BpmI   Bpu10I     BsaI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspHI    BsrBI
    BsrFI    BsrGI   BstAPI   BstEII    BstXI     BtgI   DraIII     EaeI
     EagI  EcoICRI  Eco57MI EcoO109I    Hin4I   HincII     MslI   MspA1I
     NspI     PasI     PciI     PfoI    PpuMI   PspOMI    PvuII     SacI
    SacII    SanDI    SexAI     SfcI     SmaI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI    BciVI    BfrBI
     BglI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI    BpuEI
    BsaAI    BsaBI    BsaHI    BsaWI    BsiWI     BsmI    BspEI    BspMI
    BsrDI   BssHII    BssSI    BstBI    BstYI  BstZ17I   Bsu36I    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EarI     EciI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI
     PsrI     PstI     PvuI    RsrII     SalI     SapI     SbfI     ScaI
     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI     XcmI     XhoI     XmnI     ZraI
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