Restriction map for
pF1KB5626
(Linear) (Six-base) MAPSORT of: KIBB5626.seq Check: 2886 from: 1 to: 1386
KIBB5626.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AatII G_ACGT'C
Cuts at: 0 1173 1386
Size: 1173 213
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 431 449 1386
Size: 431 18 937
Fragments arranged by size:
937 431 18
AleI CACnn'nnGTG
Cuts at: 0 385 1386
Size: 385 1001
ApaI G_GGCC'C
Cuts at: 0 1162 1386
Size: 1162 224
AvaI C'yCGr_G
Cuts at: 0 615 907 1386
Size: 615 292 479
Fragments arranged by size:
615 479 292
BanII G_rGCy'C
Cuts at: 0 97 1162 1386
Size: 97 1065 224
Fragments arranged by size:
1065 224 97
BbsI GAAGACnn'nnnn_
Cuts at: 0 437 823 1386
Size: 437 386 563
Fragments arranged by size:
563 437 386
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 732 766 826 860 1386
Size: 732 34 60 34 526
Fragments arranged by size:
732 526 60 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 483 1386
Size: 483 903
BclI T'GATC_A
Cuts at: 0 341 1386
Size: 341 1045
BfrBI ATG'CAT
Cuts at: 0 1319 1386
Size: 1319 67
BglII A'GATC_T
Cuts at: 0 1351 1386
Size: 1351 35
BlpI GC'TnA_GC
Cuts at: 0 1283 1386
Size: 1283 103
Bme1580I G_kGCm'C
Cuts at: 0 677 1162 1386
Size: 677 485 224
Fragments arranged by size:
677 485 224
BmrI ACTGGGnnnn_n'
Cuts at: 0 530 1163 1386
Size: 530 633 223
Fragments arranged by size:
633 530 223
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 72 1386
Size: 72 1314
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 341 671 939 1083 1386
Size: 341 330 268 144 303
Fragments arranged by size:
341 330 303 268 144
Bpu10I CC'TnA_GC
Cuts at: 0 1288 1386
Size: 1288 98
BsaAI yAC'GTr
Cuts at: 0 936 1386
Size: 936 450
BsaHI Gr'CG_yC
Cuts at: 0 1170 1386
Size: 1170 216
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 425 455 697 727 937 967 1032 1062
Size: 425 30 242 30 210 30 65 30
Cuts at: 1062 1386
Size: 324
Fragments arranged by size:
425 324 242 210 65 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 180 721 1244 1386
Size: 180 541 523 142
Fragments arranged by size:
541 523 180 142
BseYI C'CCAG_C
Cuts at: 0 96 1386
Size: 96 1290
Bsp1286I G_dGCh'C
Cuts at: 0 97 677 1162 1386
Size: 97 580 485 224
Fragments arranged by size:
580 485 224 97
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 136 1386
Size: 136 1250
BsrDI GCAATG_nn'
Cuts at: 0 872 1386
Size: 872 514
BsrFI r'CCGG_y
Cuts at: 0 739 1386
Size: 739 647
BssSI C'ACGA_G
Cuts at: 0 1193 1386
Size: 1193 193
BstYI r'GATC_y
Cuts at: 0 1309 1325 1351 1386
Size: 1309 16 26 35
Fragments arranged by size:
1309 35 26 16
Bsu36I CC'TnA_GG
Cuts at: 0 1213 1386
Size: 1213 173
BtgI C'CryG_G
Cuts at: 0 77 1386
Size: 77 1309
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 735 1386
Size: 735 651
EarI CTCTTCn'nnn_
Cuts at: 0 715 1386
Size: 715 671
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 718 1386
Size: 718 668
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 341 431 449 671 939 1083 1386
Size: 341 90 18 222 268 144 303
Fragments arranged by size:
341 303 268 222 144 90 18
EcoO109I rG'GnC_Cy
Cuts at: 0 1158 1189 1386
Size: 1158 31 197
Fragments arranged by size:
1158 197 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 352 384 516 548 1386
Size: 352 32 132 32 838
Fragments arranged by size:
838 352 132 32 32
MslI CAynn'nnrTG
Cuts at: 0 385 412 778 1386
Size: 385 27 366 608
Fragments arranged by size:
608 385 366 27
MspA1I CmG'CkG
Cuts at: 0 79 810 921 1061 1386
Size: 79 731 111 140 325
Fragments arranged by size:
731 325 140 111 79
NdeI CA'TA_TG
Cuts at: 0 438 1386
Size: 438 948
NsiI A_TGCA'T
Cuts at: 0 1321 1386
Size: 1321 65
NspI r_CATG'y
Cuts at: 0 1222 1323 1386
Size: 1222 101 63
Fragments arranged by size:
1222 101 63
PspOMI G'GGCC_C
Cuts at: 0 1158 1386
Size: 1158 228
PstI C_TGCA'G
Cuts at: 0 147 1386
Size: 147 1239
PvuII CAG'CTG
Cuts at: 0 921 1061 1386
Size: 921 140 325
Fragments arranged by size:
921 325 140
SacII CC_GC'GG
Cuts at: 0 80 1386
Size: 80 1306
SfcI C'TryA_G
Cuts at: 0 143 1386
Size: 143 1243
SmlI C'TyrA_G
Cuts at: 0 615 1386
Size: 615 771
SnaBI TAC'GTA
Cuts at: 0 936 1386
Size: 936 450
SpeI A'CTAG_T
Cuts at: 0 156 1386
Size: 156 1230
SphI G_CATG'C
Cuts at: 0 1222 1323 1386
Size: 1222 101 63
Fragments arranged by size:
1222 101 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 836 1057 1386
Size: 836 221 329
Fragments arranged by size:
836 329 221
TatI w'GTAC_w
Cuts at: 0 661 1386
Size: 661 725
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 425 457 997 1029 1032 1064 1386
Size: 425 32 540 32 3 32 322
Fragments arranged by size:
540 425 322 32 32 32 3
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1368 1386
Size: 1368 18
XhoI C'TCGA_G
Cuts at: 0 615 1386
Size: 615 771
XmnI GAAnn'nnTTC
Cuts at: 0 562 1386
Size: 562 824
ZraI GAC'GTC
Cuts at: 0 1171 1386
Size: 1171 215
Enzymes that do cut:
AatII AcuI AleI ApaI AvaI BanII BbsI BcgI
BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BsaAI BsaHI BsaXI BseRI BseYI Bsp1286I
BspMI BsrDI BsrFI BssSI BstYI Bsu36I BtgI BtgZI
EarI EciI Eco57MI EcoO109I Hin4I MslI MspA1I NdeI
NsiI NspI PspOMI PstI PvuII SacII SfcI SmlI
SnaBI SpeI SphI TaqII TatI TstI XcmI XhoI
XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BglI
BmgBI BmtI BpuEI BsaI BsaBI BsaWI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrGI
BssHII BstAPI BstBI BstEII BstXI BstZ17I BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
NaeI NarI NcoI NgoMIV NheI NotI NruI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SrfI SspI StuI StyI SwaI Tth111I
XbaI XmaI