Restriction map for pF1KB5625
##################################
# NO CUT ENZYMES (   87 enzymes) #
##################################

AatII       Acc65I      AflIII      AgeI        AhdI        
Alw44I      Aor51HI     ApaI        ApaLI       AscI        
Asp718I     AspEI       AviII       BamHI       BbeI        
BbrPI       BglI        BlpI        Bpu1102I    BsaAI       
BsaBI       BsiEI       BsiWI       BsmBI       BspDI       
BspLU11I    BsrBI       BssHII      Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstXI       BstZ17I     
CelII       ClaI        CpoI        DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoO65I     EcoRI       EcoT22I     FseI        FspI        
KasI        KpnI        KspI        MamI        MluI        
NaeI        NarI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PvuI        RsrII       SacII       SalI        SbfI        
SexAI       SfiI        SfoI        SfuI        SmaI        
SnaBI       SpeI        Sse8387I    SwaI        Van91I      
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   57 enzymes) #
###################################

AccII        3149  CGCG                 CG^CG
AccIII       3344  TCCGGA               T^CCGGA
AclI         3485  AACGTT               AA^CGTT
AcyI         3215  GACGCC               G[GA]^CG[CT]C
AflII        1810  CTTAAG               C^TTAAG
AocI         1202  CCTCAGG              CC^T[ACGT]AGG
AseI         2509  ATTAAT               AT^TAAT
AsnI         2509  ATTAAT               AT^TAAT
AvrII        2784  CCTAGG               C^CTAGG
BanI         1520  GGTGCC               G^G[CT][GA]CC
BbvCI         111  CCTCAGC              CCTCAGC(-5/-2)
BcgI          514  GCATCTGGCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1706  GTATCC               GTATCC(6/5)
BfrI         1810  CTTAAG               C^TTAAG
BglII        1747  AGATCT               A^GATCT
BlnI         2784  CCTAGG               C^CTAGG
BsaHI        3215  GACGCC               G[GA]^CG[CT]C
BseAI        3344  TCCGGA               T^CCGGA
BsgI         1426  GTGCAG               GTGCAG(16/14)
Bsp1407I      776  TGTACA               T^GTACA
BspEI        3344  TCCGGA               T^CCGGA
BsrDI        1765  GCAATG               GCAATG(2/0)
BsrFI        1286  ACCGGC               [GA]^CCGG[CT]
BsrGI         776  TGTACA               T^GTACA
BssSI        1004  CACGAG               CACGAG(-5/-1)
BstUI        3149  CGCG                 CG^CG
Bsu36I       1202  CCTCAGG              CC^T[ACGT]AGG
CfoI          652  GCGC                 GCG^C
Cfr10I       1286  ACCGGC               [GA]^CCGG[CT]
DraII         412  AGGGCCT              [GA]G^G[ACGT]CC[CT]
Eco81I       1202  CCTCAGG              CC^T[ACGT]AGG
EcoNI        1191  CCTCAGACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      412  AGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoRV         359  GATATC               GAT^ATC
FauI          567  CCCGC                CCCGC(4/6)
HaeII         651  GGCGCT               [GA]GCGC^[CT]
HhaI          652  GCGC                 GCG^C
Hin1I        3215  GACGCC               G[GA]^CG[CT]C
HinP1I        652  GCGC                 G^CGC
MfeI         2082  CAATTG               C^AATTG
MroI         3344  TCCGGA               T^CCGGA
MunI         2082  CAATTG               C^AATTG
MvnI         3149  CGCG                 CG^CG
NcoI         2834  CCATGG               C^CATGG
NdeI         1073  CATATG               CA^TATG
NspI          599  GCATGC               [GA]CATG^[CT]
PaeR7I       1801  CTCGAG               C^TCGAG
PshAI        2080  GACAATTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        2509  ATTAAT               AT^TAAT
Psp1406I     3485  AACGTT               AA^CGTT
PstI         1456  CTGCAG               CTGCA^G
SacI          921  GAGCTC               GAGCT^C
ScaI          719  AGTACT               AGT^ACT
SgrAI        1285  CACCGGCG             C[GA]^CCGG[CT]G
SphI          599  GCATGC               GCATG^C
SspBI         776  TGTACA               T^GTACA
XhoI         1801  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AccI          481  GTCTAC               GT^[AC][GT]AC
             2950  GTCTAC              
AfaI          720  GTAC                 GT^AC
              777  GTAC                
Asp700I       209  GAAGCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2958  GAATAGTTTC          
AspI          475  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1340  GACAGCGTC           
AvaI         1089  CCCGAG               C^[CT]CG[GA]G
             1801  CTCGAG              
AvaII        1409  GGACC                G^G[AT]CC
             2991  GGACC               
BsaI         1351  GAGACC               GGTCTC(1/5)
             3037  GAGACC              
BsoBI        1089  CCCGAG               C^[CT]CG[GA]G
             1801  CTCGAG              
BspHI        1496  TCATGA               T^CATGA
             3657  TCATGA              
BspMI        3335  ACCTGC               ACCTGC(4/8)
             3433  ACCTGC              
DraI          145  TTTAAA               TTT^AAA
             2478  TTTAAA              
DsaI         1415  CCGTGG               C^C[GA][CT]GG
             2834  CCATGG              
HincII        666  GTTAAC               GT[CT]^[GA]AC
             2464  GTTAAC              
HindII        666  GTTAAC               GT[CT]^[GA]AC
             2464  GTTAAC              
HindIII      1679  AAGCTT               A^AGCTT
             2011  AAGCTT              
HpaI          666  GTTAAC               GTT^AAC
             2464  GTTAAC              
PflFI         475  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1340  GACAGCGTC           
RcaI         1496  TCATGA               T^CATGA
             3657  TCATGA              
RsaI          720  GTAC                 GT^AC
              777  GTAC                
SapI            4  GAAGAGC              GCTCTTC(1/4)
              371  GAAGAGC             
SfcI          753  CTACAG               C^T[GA][CT]AG
             1456  CTGCAG              
Tsp45I        740  GTCAC                ^GT[GC]AC
             1634  GTGAC               
Tth111I       475  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1340  GACAGCGTC           
XbaI         2205  TCTAGA               T^CTAGA
             2278  TCTAGA              
XcmI          998  CCAGTCCACGAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2594  CCACAGCTGTGCTGG     
XmnI          209  GAAGCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2958  GAATAGTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   88 enzymes) #
#########################################

AciI        AcsI        AluI        AlwI        AlwNI       
ApoI        AspHI       BalI        BanII       BbsI        
BbvI        BclI        BcnI        BfaI        BmyI        
BpmI        BpuAI       BsaJI       BsaWI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsmI        Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       EaeI        EarI        EcoRII      EcoT14I     
FbaI        Fnu4HI      FokI        HaeIII      HapII       
HgaI        HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PleI        PvuII       Sau3AI      Sau96I      ScrFI       
SfaNI       SmlI        SspI        StuI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp509I     
TspRI       TthHB8I     XhoII       
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