Restriction map for pF1KB5625
 (Linear) (Six-base) MAPSORT of: KIBB5625.seq  Check: 9839  from: 1  to: 3759



KIBB5625.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:27  ..

AccI GT'mk_AC

Cuts at:      0     482    2951    3759 
   Size:        482    2469     808

  Fragments arranged by size: 

                2469     808     482
 
AclI AA'CG_TT

Cuts at:      0    3486    3759 
   Size:       3486     273
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     977    2029    2143    3759 
   Size:        977    1052     114    1616

  Fragments arranged by size: 

                1616    1052     977     114
 
AflII C'TTAA_G

Cuts at:      0    1810    3759 
   Size:       1810    1949
 
AleI CACnn'nnGTG

Cuts at:      0    2599    3759 
   Size:       2599    1160
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3152    3759 
   Size:       3152     607
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     531     563    3759 
   Size:        531      32    3196

  Fragments arranged by size: 

                3196     531      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1347    1367    2241    2938    3759 
   Size:       1347      20     874     697     821

  Fragments arranged by size: 

                1347     874     821     697      20
 
ApoI r'AATT_y

Cuts at:      0    1165    2291    2917    3759 
   Size:       1165    1126     626     842

  Fragments arranged by size: 

                1165    1126     842     626
 
AseI AT'TA_AT

Cuts at:      0    2510    3759 
   Size:       2510    1249
 
AvaI C'yCGr_G

Cuts at:      0    1089    1801    3759 
   Size:       1089     712    1958

  Fragments arranged by size: 

                1958    1089     712
 
AvrII C'CTAG_G

Cuts at:      0    2784    3759 
   Size:       2784     975
 
BanI G'GyrC_C

Cuts at:      0    1520    3759 
   Size:       1520    2239
 
BanII G_rGCy'C

Cuts at:      0     925    1504    3198    3759 
   Size:        925     579    1694     561

  Fragments arranged by size: 

                1694     925     579     561
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      65    2074    2174    3359    3436    3759 
   Size:         65    2009     100    1185      77     323

  Fragments arranged by size: 

                2009    1185     323     100      77      65
 
BbvCI CC'TCA_GC

Cuts at:      0     112    3759 
   Size:        112    3647
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     503     537    3759 
   Size:        503      34    3222

  Fragments arranged by size: 

                3222     503      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1717    3759 
   Size:       1717    2042
 
BclI T'GATC_A

Cuts at:      0     384    1062    1836    3597    3759 
   Size:        384     678     774    1761     162

  Fragments arranged by size: 

                1761     774     678     384     162
 
BglII A'GATC_T

Cuts at:      0    1747    3759 
   Size:       1747    2012
 
Bme1580I G_kGCm'C

Cuts at:      0    3312    3759 
   Size:       3312     447
 
BmgBI CAC'GTC

Cuts at:      0    1264    3759 
   Size:       1264    2495
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1415    3759 
   Size:       1415    2344
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      78    2073    3463    3759 
   Size:         78    1995    1390     296

  Fragments arranged by size: 

                1995    1390     296      78
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     708    1130    1369    2194    2418    3759 
   Size:        708     422     239     825     224    1341

  Fragments arranged by size: 

                1341     825     708     422     239     224
 
Bpu10I CC'TnA_GC

Cuts at:      0     112     252     924    3759 
   Size:        112     140     672    2835

  Fragments arranged by size: 

                2835     672     140     112
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     438     441    1468    3589    3759 
   Size:        438       3    1027    2121     170

  Fragments arranged by size: 

                2121    1027     438     170       3
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1345    3031    3759 
   Size:       1345    1686     728

  Fragments arranged by size: 

                1686    1345     728
 
BsaHI Gr'CG_yC

Cuts at:      0    3216    3759 
   Size:       3216     543
 
BsaWI w'CCGG_w

Cuts at:      0    1526    1952    3344    3759 
   Size:       1526     426    1392     415

  Fragments arranged by size: 

                1526    1392     426     415
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1465    1495    1560    1590    1632    1662    3759 
   Size:       1465      30      65      30      42      30    2097

  Fragments arranged by size: 

                2097    1465      65      42      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1029    1486    1640    3759 
   Size:       1029     457     154    2119

  Fragments arranged by size: 

                2119    1029     457     154
 
BseYI C'CCAG_C

Cuts at:      0    1214    2072    3759 
   Size:       1214     858    1687

  Fragments arranged by size: 

                1687    1214     858
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1447    3759 
   Size:       1447    2312
 
BsiHKAI G_wGCw'C

Cuts at:      0     925    1612    1881    2633    2816    3759 
   Size:        925     687     269     752     183     943

  Fragments arranged by size: 

                 943     925     752     687     269     183
 
BsmI GAATG_Cn'

Cuts at:      0      72     635    3615    3759 
   Size:         72     563    2980     144

  Fragments arranged by size: 

                2980     563     144      72
 
Bsp1286I G_dGCh'C

Cuts at:      0     925    1504    1612    1881    2633    2816    3198    3312 
   Size:        925     579     108     269     752     183     382     114

Cuts at:   3312    3759 
   Size:        447

  Fragments arranged by size: 

                 925     752     579     447     382     269     183     114
                 108
 
BspEI T'CCGG_A

Cuts at:      0    3344    3759 
   Size:       3344     415
 
BspHI T'CATG_A

Cuts at:      0    1496    3657    3759 
   Size:       1496    2161     102

  Fragments arranged by size: 

                2161    1496     102
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    3344    3442    3759 
   Size:       3344      98     317

  Fragments arranged by size: 

                3344     317      98
 
BsrDI GCAATG_nn'

Cuts at:      0    1772    3759 
   Size:       1772    1987
 
BsrFI r'CCGG_y

Cuts at:      0    1286    3759 
   Size:       1286    2473
 
BsrGI T'GTAC_A

Cuts at:      0     776    3759 
   Size:        776    2983
 
BssSI C'ACGA_G

Cuts at:      0    1004    3759 
   Size:       1004    2755
 
BstYI r'GATC_y

Cuts at:      0     867    1156    1747    3704    3759 
   Size:        867     289     591    1957      55

  Fragments arranged by size: 

                1957     867     591     289      55
 
Bsu36I CC'TnA_GG

Cuts at:      0    1203    3759 
   Size:       1203    2556
 
BtgI C'CryG_G

Cuts at:      0    1415    2834    3759 
   Size:       1415    1419     925

  Fragments arranged by size: 

                1419    1415     925
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    3671    3759 
   Size:       3671      88
 
BtsI GCAGTG_nn'

Cuts at:      0    1590    2745    3759 
   Size:       1590    1155    1014

  Fragments arranged by size: 

                1590    1155    1014
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    3441    3476    3759 
   Size:       3441      35     283

  Fragments arranged by size: 

                3441     283      35
 
DraI TTT'AAA

Cuts at:      0     147    2480    3759 
   Size:        147    2333    1279

  Fragments arranged by size: 

                2333    1279     147
 
EaeI y'GGCC_r

Cuts at:      0     396     530    2837    3387    3759 
   Size:        396     134    2307     550     372

  Fragments arranged by size: 

                2307     550     396     372     134
 
EarI CTCTTCn'nnn_

Cuts at:      0     135     366    2119    3759 
   Size:        135     231    1753    1640

  Fragments arranged by size: 

                1753    1640     231     135
 
EcoICRI GAG'CTC

Cuts at:      0     923    3759 
   Size:        923    2836
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     708     977    1130    1369    2029    2143    2194    2418 
   Size:        708     269     153     239     660     114      51     224

Cuts at:   2418    3759 
   Size:       1341

  Fragments arranged by size: 

                1341     708     660     269     239     224     153     114
                  51
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1195    3759 
   Size:       1195    2564
 
EcoO109I rG'GnC_Cy

Cuts at:      0     413    3759 
   Size:        413    3346
 
EcoRV GAT'ATC

Cuts at:      0     361    3759 
   Size:        361    3398
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     620    2241    3372    3759 
   Size:        620    1621    1131     387

  Fragments arranged by size: 

                1621    1131     620     387
 
HaeII r_GCGC'y

Cuts at:      0     655    3759 
   Size:        655    3104
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     673     705    1266    1298    1329    1361    1459    1491 
   Size:        673      32     561      32      31      32      98      32

Cuts at:   1491    1560    1592    1926    1958    2836    2868    3055    3087 
   Size:         69      32     334      32     878      32     187      32

Cuts at:   3087    3434    3466    3612    3644    3759 
   Size:        347      32     146      32     115

  Fragments arranged by size: 

                 878     673     561     347     334     187     146     115
                  98      69      32      32      32      32      32      32
                  32      32      32      32      31
 
HincII GTy'rAC

Cuts at:      0     668    2466    3759 
   Size:        668    1798    1293

  Fragments arranged by size: 

                1798    1293     668
 
HindIII A'AGCT_T

Cuts at:      0    1679    2011    3759 
   Size:       1679     332    1748

  Fragments arranged by size: 

                1748    1679     332
 
HpaI GTT'AAC

Cuts at:      0     668    2466    3759 
   Size:        668    1798    1293

  Fragments arranged by size: 

                1798    1293     668
 
MfeI C'AATT_G

Cuts at:      0    2082    3759 
   Size:       2082    1677
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     346     487    1844    2123    3759 
   Size:        346     141    1357     279    1636

  Fragments arranged by size: 

                1636    1357     346     279     141
 
MscI TGG'CCA

Cuts at:      0     532    2839    3389    3759 
   Size:        532    2307     550     370

  Fragments arranged by size: 

                2307     550     532     370
 
MslI CAynn'nnrTG

Cuts at:      0     476    2599    3454    3759 
   Size:        476    2123     855     305

  Fragments arranged by size: 

                2123     855     476     305
 
MspA1I CmG'CkG

Cuts at:      0     798    2076    2599    2801    3759 
   Size:        798    1278     523     202     958

  Fragments arranged by size: 

                1278     958     798     523     202
 
NcoI C'CATG_G

Cuts at:      0    2834    3759 
   Size:       2834     925
 
NdeI CA'TA_TG

Cuts at:      0    1074    3759 
   Size:       1074    2685
 
NspI r_CATG'y

Cuts at:      0     603    3759 
   Size:        603    3156
 
PfoI T'CCnGG_A

Cuts at:      0    3227    3759 
   Size:       3227     532
 
PshAI GACnn'nnGTC

Cuts at:      0    2084    3759 
   Size:       2084    1675
 
PsiI TTA'TAA

Cuts at:      0    2876    2924    3759 
   Size:       2876      48     835

  Fragments arranged by size: 

                2876     835      48
 
PspXI vC'TCGA_Gb

Cuts at:      0    1801    3759 
   Size:       1801    1958
 
PstI C_TGCA'G

Cuts at:      0    1460    3759 
   Size:       1460    2299
 
PvuII CAG'CTG

Cuts at:      0     798    2076    2599    2801    3759 
   Size:        798    1278     523     202     958

  Fragments arranged by size: 

                1278     958     798     523     202
 
SacI G_AGCT'C

Cuts at:      0     925    3759 
   Size:        925    2834
 
SapI GCTCTTCn'nnn_

Cuts at:      0     366    3759 
   Size:        366    3393
 
ScaI AGT'ACT

Cuts at:      0     721    3759 
   Size:        721    3038
 
SfcI C'TryA_G

Cuts at:      0     753    1456    3759 
   Size:        753     703    2303

  Fragments arranged by size: 

                2303     753     703
 
SgrAI Cr'CCGG_yG

Cuts at:      0    1286    3759 
   Size:       1286    2473
 
SmlI C'TyrA_G

Cuts at:      0     417     456    1447    1801    1810    3604    3759 
   Size:        417      39     991     354       9    1794     155

  Fragments arranged by size: 

                1794     991     417     354     155      39       9
 
SphI G_CATG'C

Cuts at:      0     603    3759 
   Size:        603    3156
 
SspI AAT'ATT

Cuts at:      0     306    3093    3396    3759 
   Size:        306    2787     303     363

  Fragments arranged by size: 

                2787     363     306     303
 
StuI AGG'CCT

Cuts at:      0    1201    2675    2998    3759 
   Size:       1201    1474     323     761

  Fragments arranged by size: 

                1474    1201     761     323
 
StyI C'CwwG_G

Cuts at:      0    1997    2784    2834    3759 
   Size:       1997     787      50     925

  Fragments arranged by size: 

                1997     925     787      50
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    3448    3759 
   Size:       3448     311
 
TatI w'GTAC_w

Cuts at:      0     719     776    3759 
   Size:        719      57    2983

  Fragments arranged by size: 

                2983     719      57
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1632    1664    2541    2573    3759 
   Size:       1632      32     877      32    1186

  Fragments arranged by size: 

                1632    1186     877      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     478    1343    3759 
   Size:        478     865    2416

  Fragments arranged by size: 

                2416     865     478
 
XbaI T'CTAG_A

Cuts at:      0    2205    2278    3759 
   Size:       2205      73    1481

  Fragments arranged by size: 

                2205    1481      73
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1005    2601    3759 
   Size:       1005    1596    1158

  Fragments arranged by size: 

                1596    1158    1005
 
XhoI C'TCGA_G

Cuts at:      0    1801    3759 
   Size:       1801    1958
 
XmnI GAAnn'nnTTC

Cuts at:      0     213    2962    3759 
   Size:        213    2749     797

  Fragments arranged by size: 

                2749     797     213
 

 Enzymes that do cut:

     AccI     AclI     AcuI    AflII     AleI     AlfI     AloI    AlwNI
     ApoI     AseI     AvaI    AvrII     BanI    BanII     BbsI    BbvCI
     BcgI    BciVI     BclI    BglII Bme1580I    BmgBI     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI
    BsrDI    BsrFI    BsrGI    BssSI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI    CspCI     DraI     EaeI     EarI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRV     FalI    HaeII    Hin4I   HincII  HindIII     HpaI
     MfeI     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NspI
     PfoI    PshAI     PsiI    PspXI     PstI    PvuII     SacI     SapI
     ScaI     SfcI    SgrAI     SmlI     SphI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AfeI   AflIII     AgeI     AhdI     ApaI
    ApaLI     AscI    AsiSI     BaeI    BamHI     BbeI    BfrBI     BglI
     BlpI     BmtI    BsaAI    BsaBI    BsiEI    BsiWI    BsmBI    BsrBI
   BssHII   BstAPI    BstBI   BstEII    BstXI  BstZ17I     ClaI   DraIII
     DrdI     EagI     EciI    EcoRI     FseI     FspI    FspAI     KasI
     KpnI     MluI     NaeI     NarI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI
    PpuMI   PspOMI     PsrI     PvuI    RsrII    SacII     SalI    SanDI
     SbfI    SexAI     SfiI     SfoI     SgfI     SmaI    SnaBI     SpeI
     SrfI     SwaI     XmaI     ZraI
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