Restriction map for pF1KB5599
 (Linear) (Six-base) MAPSORT of: KIBB5599.seq  Check: 1765  from: 1  to: 2796



KIBB5599.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:27  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1065    2242    2796 
   Size:       1065    1177     554

  Fragments arranged by size: 

                1177    1065     554
 
Acc65I G'GTAC_C

Cuts at:      0     688    2796 
   Size:        688    2108
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1795    1886    2796 
   Size:       1795      91     910

  Fragments arranged by size: 

                1795     910      91
 
AleI CACnn'nnGTG

Cuts at:      0     103     340    2796 
   Size:        103     237    2456

  Fragments arranged by size: 

                2456     237     103
 
AlwNI CAG_nnn'CTG

Cuts at:      0     658    1932    2677    2796 
   Size:        658    1274     745     119

  Fragments arranged by size: 

                1274     745     658     119
 
ApoI r'AATT_y

Cuts at:      0     304     364     703    1213    1293    2511    2520    2796 
   Size:        304      60     339     510      80    1218       9     276

  Fragments arranged by size: 

                1218     510     339     304     276      80      60       9
            
 
AseI AT'TA_AT

Cuts at:      0     614    2796 
   Size:        614    2182
 
BanI G'GyrC_C

Cuts at:      0     688    2796 
   Size:        688    2108
 
BanII G_rGCy'C

Cuts at:      0     128    1194    1643    2796 
   Size:        128    1066     449    1153

  Fragments arranged by size: 

                1153    1066     449     128
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1285    1439    2492    2796 
   Size:       1285     154    1053     304

  Fragments arranged by size: 

                1285    1053     304     154
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2259    2796 
   Size:       2259     537
 
BclI T'GATC_A

Cuts at:      0     180    2490    2796 
   Size:        180    2310     306

  Fragments arranged by size: 

                2310     306     180
 
Bme1580I G_kGCm'C

Cuts at:      0     476    2796 
   Size:        476    2320
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1197    2796 
   Size:       1197    1599
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     854    2198    2796 
   Size:        854    1344     598

  Fragments arranged by size: 

                1344     854     598
 
Bpu10I CC'TnA_GC

Cuts at:      0    1757    2796 
   Size:       1757    1039
 
BsaBI GATnn'nnATC

Cuts at:      0    2387    2690    2796 
   Size:       2387     303     106

  Fragments arranged by size: 

                2387     303     106
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     827     857    2208    2238    2796 
   Size:        827      30    1351      30     558

  Fragments arranged by size: 

                1351     827     558      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     154    1708    2353    2362    2796 
   Size:        154    1554     645       9     434

  Fragments arranged by size: 

                1554     645     434     154       9
 
BseYI C'CCAG_C

Cuts at:      0      41    2705    2796 
   Size:         41    2664      91

  Fragments arranged by size: 

                2664      91      41
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     704    2796 
   Size:        704    2092
 
BsiEI CG_ry'CG

Cuts at:      0      18      77    2796 
   Size:         18      59    2719

  Fragments arranged by size: 

                2719      59      18
 
BsiHKAI G_wGCw'C

Cuts at:      0    2363    2796 
   Size:       2363     433
 
BsmI GAATG_Cn'

Cuts at:      0    1032    2178    2796 
   Size:       1032    1146     618

  Fragments arranged by size: 

                1146    1032     618
 
Bsp1286I G_dGCh'C

Cuts at:      0     128     476    1194    1643    2363    2796 
   Size:        128     348     718     449     720     433

  Fragments arranged by size: 

                 720     718     449     433     348     128
 
BspHI T'CATG_A

Cuts at:      0    1113    1359    2796 
   Size:       1113     246    1437

  Fragments arranged by size: 

                1437    1113     246
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     676    1065    1107    2242    2796 
   Size:        676     389      42    1135     554

  Fragments arranged by size: 

                1135     676     554     389      42
 
BsrFI r'CCGG_y

Cuts at:      0      10     573    2796 
   Size:         10     563    2223

  Fragments arranged by size: 

                2223     563      10
 
BsrGI T'GTAC_A

Cuts at:      0     673    1947    2638    2796 
   Size:        673    1274     691     158

  Fragments arranged by size: 

                1274     691     673     158
 
BssSI C'ACGA_G

Cuts at:      0    1940    2796 
   Size:       1940     856
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2176    2796 
   Size:       2176     620
 
BstYI r'GATC_y

Cuts at:      0    1254    1595    2016    2282    2388    2796 
   Size:       1254     341     421     266     106     408

  Fragments arranged by size: 

                1254     421     408     341     266     106
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      66    2796 
   Size:         66    2730
 
BtsI GCAGTG_nn'

Cuts at:      0     383     523    2108    2229    2796 
   Size:        383     140    1585     121     567

  Fragments arranged by size: 

                1585     567     383     140     121
 
DraI TTT'AAA

Cuts at:      0     350     620    1298    1401    2796 
   Size:        350     270     678     103    1395

  Fragments arranged by size: 

                1395     678     350     270     103
 
EaeI y'GGCC_r

Cuts at:      0      15      74      95    1745    2796 
   Size:         15      59      21    1650    1051

  Fragments arranged by size: 

                1650    1051      59      21      15
 
EagI C'GGCC_G

Cuts at:      0      15      74    2796 
   Size:         15      59    2722

  Fragments arranged by size: 

                2722      59      15
 
EarI CTCTTCn'nnn_

Cuts at:      0     995    2796 
   Size:        995    1801
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     854    1795    1886    2198    2796 
   Size:        854     941      91     312     598

  Fragments arranged by size: 

                 941     854     598     312      91
 
EcoO109I rG'GnC_Cy

Cuts at:      0      29    2796 
   Size:         29    2767
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1667    1699    2482    2514    2580    2607    2612    2639 
   Size:       1667      32     783      32      66      27       5      27

Cuts at:   2639    2796 
   Size:        157

  Fragments arranged by size: 

                1667     783     157      66      32      32      27      27
                   5
 
HindIII A'AGCT_T

Cuts at:      0     151    2796 
   Size:        151    2645
 
KpnI G_GTAC'C

Cuts at:      0     692    2796 
   Size:        692    2104
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     843    1854    2796 
   Size:        843    1011     942

  Fragments arranged by size: 

                1011     942     843
 
MscI TGG'CCA

Cuts at:      0    1747    2796 
   Size:       1747    1049
 
MslI CAynn'nnrTG

Cuts at:      0     103     188     340     956    2796 
   Size:        103      85     152     616    1840

  Fragments arranged by size: 

                1840     616     152     103      85
 
MspA1I CmG'CkG

Cuts at:      0    1932    2796 
   Size:       1932     864
 
NaeI GCC'GGC

Cuts at:      0      12    2796 
   Size:         12    2784
 
NgoMIV G'CCGG_C

Cuts at:      0      10    2796 
   Size:         10    2786
 
NspI r_CATG'y

Cuts at:      0     211    1837    2145    2796 
   Size:        211    1626     308     651

  Fragments arranged by size: 

                1626     651     308     211
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1972    2071    2796 
   Size:       1972      99     725

  Fragments arranged by size: 

                1972     725      99
 
PfoI T'CCnGG_A

Cuts at:      0    2336    2796 
   Size:       2336     460
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1522    1554    2796 
   Size:       1522      32    1242

  Fragments arranged by size: 

                1522    1242      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      29    2796 
   Size:         29    2767
 
PvuII CAG'CTG

Cuts at:      0    1932    2796 
   Size:       1932     864
 
SanDI GG'GwC_CC

Cuts at:      0      29    2796 
   Size:         29    2767
 
ScaI AGT'ACT

Cuts at:      0     167    2796 
   Size:        167    2629
 
SfcI C'TryA_G

Cuts at:      0     243    1247    2726    2796 
   Size:        243    1004    1479      70

  Fragments arranged by size: 

                1479    1004     243      70
 
SpeI A'CTAG_T

Cuts at:      0      60    2796 
   Size:         60    2736
 
SspI AAT'ATT

Cuts at:      0     740     825    2796 
   Size:        740      85    1971

  Fragments arranged by size: 

                1971     740      85
 
StyI C'CwwG_G

Cuts at:      0     296    2796 
   Size:        296    2500
 
SwaI ATTT'AAAT

Cuts at:      0    1298    2796 
   Size:       1298    1498
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     457    1979    2543    2796 
   Size:        457    1522     564     253

  Fragments arranged by size: 

                1522     564     457     253
 
TatI w'GTAC_w

Cuts at:      0     165     673    1182    1947    2638    2796 
   Size:        165     508     509     765     691     158

  Fragments arranged by size: 

                 765     691     509     508     165     158
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2150    2182    2208    2240    2796 
   Size:       2150      32      26      32     556

  Fragments arranged by size: 

                2150     556      32      32      26
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1740    1969    2796 
   Size:       1740     229     827

  Fragments arranged by size: 

                1740     827     229
 
XmnI GAAnn'nnTTC

Cuts at:      0     306    1374    2796 
   Size:        306    1068    1422

  Fragments arranged by size: 

                1422    1068     306
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI     AleI    AlwNI     ApoI     AseI     BanI
    BanII     BbsI    BciVI     BclI Bme1580I     BmrI     BpmI   Bpu10I
    BsaBI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspHI    BspMI    BsrFI    BsrGI    BssSI    BstXI    BstYI
    BtgZI     BtsI     DraI     EaeI     EagI     EarI  Eco57MI EcoO109I
    Hin4I  HindIII     KpnI     MmeI     MscI     MslI   MspA1I     NaeI
   NgoMIV     NspI    PflMI     PfoI     PpiI    PpuMI    PvuII    SanDI
     ScaI     SfcI     SpeI     SspI     StyI     SwaI    TaqII     TatI
     TstI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BbeI    BbvCI     BcgI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaHI
    BsaWI    BsiWI    BsmBI    BspEI    BsrBI    BsrDI   BssHII   BstAPI
    BstBI   BstEII  BstZ17I   Bsu36I     BtgI     ClaI    CspCI   DraIII
     DrdI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII   HincII     HpaI     KasI     MfeI     MluI
     NarI     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI     PmeI     PmlI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI     SapI
     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SphI     SrfI     StuI  Tth111I     XbaI     XhoI     XmaI
     ZraI
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