Restriction map for pF1KB5567
##################################
# NO CUT ENZYMES (  122 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AgeI        AhdI        AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BamHI       BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BcnI        BglI        
BlnI        BlpI        Bpu1102I    BseRI       BsiEI       
BsiWI       BsoBI       Bsp1407I    BspDI       BsrBI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstUI       BstZ17I     
CelII       Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        DsaI        EagI        Eam1105I    
EclXI       Eco52I      EcoNI       EcoO65I     EcoRI       
EcoT22I     FauI        FbaI        FseI        FspI        
HincII      HindII      HindIII     HpaI        KasI        
KpnI        KspI        MfeI        MluI        MslI        
MunI        MvnI        NaeI        NarI        NciI        
NcoI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
SwaI        Tsp45I      Tth111I     XbaI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AcyI          597  GGCGTC               G[GA]^CG[CT]C
AflII        1128  CTTAAG               C^TTAAG
AflIII        129  ACATGT               A^C[GA][CT]GT
Alw44I        926  GTGCAC               G^TGCAC
Aor51HI      1616  AGCGCT               AGC^GCT
ApaI         1509  GGGCCC               GGGCC^C
ApaLI         926  GTGCAC               G^TGCAC
Asp700I        85  GAAGCTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI          721  TGGCCA               TGG^CCA
BanI          705  GGCACC               G^G[CT][GA]CC
BfrI         1128  CTTAAG               C^TTAAG
BglII           4  AGATCT               A^GATCT
BsaAI        1324  CACGTA               [CT]AC^GT[GA]
BsaBI         641  GATTAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         597  GGCGTC               G[GA]^CG[CT]C
BsgI         1694  GTGCAG               GTGCAG(16/14)
BspHI         849  TCATGA               T^CATGA
BspLU11I      129  ACATGT               A^CATGT
BspMI        1663  GCAGGT               ACCTGC(4/8)
BsrDI        1348  GCAATG               GCAATG(2/0)
BstAPI        928  GCACAGCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI        1949  CCAAGAGAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EaeI          721  TGGCCA               [CT]^GGCC[GA]
Eco47III     1616  AGCGCT               AGC^GCT
EcoRV        1355  GATATC               GAT^ATC
HgaI          598  GCGTC                GACGC(5/10)
Hin1I         597  GGCGTC               G[GA]^CG[CT]C
MaeII        1325  ACGT                 A^CGT
MamI          641  GATTAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         721  TGGCCA               TGG^CCA
MscI          721  TGGCCA               TGG^CCA
PleI          692  GAGTC                GAGTC(4/5)
PpuMI         837  GGGTCCT              [GA]G^G[AT]CC[CT]
PstI         1802  CTGCAG               CTGCA^G
RcaI          849  TCATGA               T^CATGA
SacI         1549  GAGCTC               GAGCT^C
SphI          933  GCATGC               GCATG^C
TfiI          384  GATTC                G^A[AT]TC
XcmI          709  CCATTGAAAGGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI           85  GAAGCTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AccIII         72  TCCGGA               T^CCGGA
              558  TCCGGA              
AfaI          755  GTAC                 GT^AC
             1487  GTAC                
AlwNI        1028  CAGTTACTG            CAG[ACGT][ACGT][ACGT]^CTG
             1592  CAGAGACTG           
AocI         1292  CCTCAGG              CC^T[ACGT]AGG
             1768  CCTCAGG             
BfaI          727  CTAG                 C^TAG
              998  CTAG                
BsaWI          72  TCCGGA               [AT]^CCGG[AT]
              558  TCCGGA              
BseAI          72  TCCGGA               T^CCGGA
              558  TCCGGA              
BsmBI         599  CGTCTC               CGTCTC(1/5)
              792  GAGACG              
BsmFI         509  GGGAC                GGGAC(10/14)
              694  GTCCC               
BsmI         1167  GAATGC               GAATGC(1/-1)
              668  GCATTC              
BspEI          72  TCCGGA               T^CCGGA
              558  TCCGGA              
BstYI           4  AGATCT               [GA]^GATC[CT]
              552  AGATCC              
Bsu36I       1292  CCTCAGG              CC^T[ACGT]AGG
             1768  CCTCAGG             
Eco81I       1292  CCTCAGG              CC^T[ACGT]AGG
             1768  CCTCAGG             
HaeII         775  GGCGCT               [GA]GCGC^[CT]
             1616  AGCGCT              
HinfI         384  GATTC                G^A[ACGT]TC
              692  GAGTC               
MaeI          727  CTAG                 C^TAG
              998  CTAG                
MflI            4  AGATCT               [GA]^GATC[CT]
              552  AGATCC              
MroI           72  TCCGGA               T^CCGGA
              558  TCCGGA              
NdeI         1283  CATATG               CA^TATG
             1910  CATATG              
PflMI          36  CCATGTTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1894  CCAGGCTATGG         
RsaI          755  GTAC                 GT^AC
             1487  GTAC                
StuI          785  AGGCCT               AGG^CCT
             1195  AGGCCT              
TspRI         924  CAGTG                CA[GC]TG[ACGT][ACGT]^
             1433  CAGTG               
Van91I         36  CCATGTTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1894  CCAGGCTATGG         
XhoII           4  AGATCT               [GA]^GATC[CT]
              552  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   70 enzymes) #
#########################################

AciI        AcsI        AluI        AlwI        ApoI        
AspHI       AvaII       BanII       BbsI        BbvI        
BmyI        BpmI        BpuAI       BsaI        BsaJI       
BsiHKAI     BsiYI       BslI        BsmAI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
EarI        EcoO109I    EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HpaII       HphI        ItaI        Ksp632I     MaeIII      
MboI        MboII       MnlI        MseI        MspA1I      
MspI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       NspI        PvuII       Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StyI        
TaqI        Tru9I       TseI        Tsp509I     TthHB8I     
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