Restriction map for pF1KB5555
 (Linear) (Six-base) MAPSORT of: KIBB5555.seq  Check: 5782  from: 1  to: 1356



KIBB5555.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:27  ..

AhdI GACnn_n'nnGTC

Cuts at:      0      45     809    1356 
   Size:         45     764     547

  Fragments arranged by size: 

                 764     547      45
 
AleI CACnn'nnGTG

Cuts at:      0     553    1327    1356 
   Size:        553     774      29

  Fragments arranged by size: 

                 774     553      29
 
AlwNI CAG_nnn'CTG

Cuts at:      0     795     959    1104    1356 
   Size:        795     164     145     252

  Fragments arranged by size: 

                 795     252     164     145
 
ApaI G_GGCC'C

Cuts at:      0     802    1014    1356 
   Size:        802     212     342

  Fragments arranged by size: 

                 802     342     212
 
ApaLI G'TGCA_C

Cuts at:      0    1051    1356 
   Size:       1051     305
 
ApoI r'AATT_y

Cuts at:      0     702    1075    1356 
   Size:        702     373     281

  Fragments arranged by size: 

                 702     373     281
 
AvaI C'yCGr_G

Cuts at:      0     108    1356 
   Size:        108    1248
 
BamHI G'GATC_C

Cuts at:      0     223    1356 
   Size:        223    1133
 
BanII G_rGCy'C

Cuts at:      0     802    1014    1356 
   Size:        802     212     342

  Fragments arranged by size: 

                 802     342     212
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     659    1356 
   Size:        659     697
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      85     119    1356 
   Size:         85      34    1237

  Fragments arranged by size: 

                1237      85      34
 
BlpI GC'TnA_GC

Cuts at:      0    1003    1356 
   Size:       1003     353
 
Bme1580I G_kGCm'C

Cuts at:      0     741     802    1014    1055    1356 
   Size:        741      61     212      41     301

  Fragments arranged by size: 

                 741     301     212      61      41
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     888    1356 
   Size:        888     468
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     445     883    1221    1356 
   Size:        445     438     338     135

  Fragments arranged by size: 

                 445     438     338     135
 
BsaAI yAC'GTr

Cuts at:      0    1329    1356 
   Size:       1329      27
 
BsaBI GATnn'nnATC

Cuts at:      0     457     785    1356 
   Size:        457     328     571

  Fragments arranged by size: 

                 571     457     328
 
BsaWI w'CCGG_w

Cuts at:      0     237    1356 
   Size:        237    1119
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      10      40    1255    1285    1356 
   Size:         10      30    1215      30      71

  Fragments arranged by size: 

                1215      71      30      30      10
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     329     378     695    1356 
   Size:        329      49     317     661

  Fragments arranged by size: 

                 661     329     317      49
 
BseYI C'CCAG_C

Cuts at:      0     773     959    1356 
   Size:        773     186     397

  Fragments arranged by size: 

                 773     397     186
 
BsiEI CG_ry'CG

Cuts at:      0    1121    1356 
   Size:       1121     235
 
BsiHKAI G_wGCw'C

Cuts at:      0    1055    1356 
   Size:       1055     301
 
BsiWI C'GTAC_G

Cuts at:      0      65    1356 
   Size:         65    1291
 
Bsp1286I G_dGCh'C

Cuts at:      0     741     802    1014    1055    1356 
   Size:        741      61     212      41     301

  Fragments arranged by size: 

                 741     301     212      61      41
 
BsrBI CCG'CTC

Cuts at:      0      73     969    1356 
   Size:         73     896     387

  Fragments arranged by size: 

                 896     387      73
 
BsrDI GCAATG_nn'

Cuts at:      0     424    1091    1356 
   Size:        424     667     265

  Fragments arranged by size: 

                 667     424     265
 
BsrGI T'GTAC_A

Cuts at:      0    1133    1356 
   Size:       1133     223
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     220    1356 
   Size:        220    1136
 
BstYI r'GATC_y

Cuts at:      0     223     780     791     839    1356 
   Size:        223     557      11      48     517

  Fragments arranged by size: 

                 557     517     223      48      11
 
Bsu36I CC'TnA_GG

Cuts at:      0     580    1356 
   Size:        580     776
 
BtgI C'CryG_G

Cuts at:      0    1086    1277    1356 
   Size:       1086     191      79

  Fragments arranged by size: 

                1086     191      79
 
BtsI GCAGTG_nn'

Cuts at:      0     592    1356 
   Size:        592     764
 
DraIII CAC_nnn'GTG

Cuts at:      0     395    1356 
   Size:        395     961
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1125    1356 
   Size:       1125     231
 
EaeI y'GGCC_r

Cuts at:      0     950    1356 
   Size:        950     406
 
EarI CTCTTCn'nnn_

Cuts at:      0     672    1356 
   Size:        672     684
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     293    1356 
   Size:        293    1063
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     445     883    1221    1356 
   Size:        445     438     338     135

  Fragments arranged by size: 

                 445     438     338     135
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1011    1356 
   Size:       1011     345
 
EcoRI G'AATT_C

Cuts at:      0     702    1356 
   Size:        702     654
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     537     701    1085    1212    1356 
   Size:        537     164     384     127     144

  Fragments arranged by size: 

                 537     384     164     144     127
 
HaeII r_GCGC'y

Cuts at:      0     884     960    1356 
   Size:        884      76     396

  Fragments arranged by size: 

                 884     396      76
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      10      31      42      63     359     391     616     629 
   Size:         10      21      11      21     296      32     225      13

Cuts at:    629     648     661     795     827    1356 
   Size:         19      13     134      32     529

  Fragments arranged by size: 

                 529     296     225     134      32      32      21      21
                  19      13      13      11      10
 
HincII GTy'rAC

Cuts at:      0     192    1356 
   Size:        192    1164
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     314     899    1141    1356 
   Size:        314     585     242     215

  Fragments arranged by size: 

                 585     314     242     215
 
MscI TGG'CCA

Cuts at:      0     952    1356 
   Size:        952     404
 
MslI CAynn'nnrTG

Cuts at:      0     218     553    1327    1356 
   Size:        218     335     774      29

  Fragments arranged by size: 

                 774     335     218      29
 
MspA1I CmG'CkG

Cuts at:      0     846     893    1356 
   Size:        846      47     463

  Fragments arranged by size: 

                 846     463      47
 
NspI r_CATG'y

Cuts at:      0     412    1164    1356 
   Size:        412     752     192

  Fragments arranged by size: 

                 752     412     192
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     395     483     959    1356 
   Size:        395      88     476     397

  Fragments arranged by size: 

                 476     397     395      88
 
PfoI T'CCnGG_A

Cuts at:      0     860    1356 
   Size:        860     496
 
PmlI CAC'GTG

Cuts at:      0    1329    1356 
   Size:       1329      27
 
PspOMI G'GGCC_C

Cuts at:      0     798    1010    1356 
   Size:        798     212     346

  Fragments arranged by size: 

                 798     346     212
 
PstI C_TGCA'G

Cuts at:      0     250    1109    1356 
   Size:        250     859     247

  Fragments arranged by size: 

                 859     250     247
 
PvuII CAG'CTG

Cuts at:      0     846     893    1356 
   Size:        846      47     463

  Fragments arranged by size: 

                 846     463      47
 
SfcI C'TryA_G

Cuts at:      0     246    1105    1356 
   Size:        246     859     251

  Fragments arranged by size: 

                 859     251     246
 
StyI C'CwwG_G

Cuts at:      0      89     729    1356 
   Size:         89     640     627

  Fragments arranged by size: 

                 640     627      89
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1135    1339    1356 
   Size:       1135     204      17

  Fragments arranged by size: 

                1135     204      17
 
TatI w'GTAC_w

Cuts at:      0     443    1133    1356 
   Size:        443     690     223

  Fragments arranged by size: 

                 690     443     223
 
Tth111I GACn'n_nGTC

Cuts at:      0     670    1122    1356 
   Size:        670     452     234

  Fragments arranged by size: 

                 670     452     234
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     164     484    1356 
   Size:        164     320     872

  Fragments arranged by size: 

                 872     320     164
 

 Enzymes that do cut:

     AhdI     AleI    AlwNI     ApaI    ApaLI     ApoI     AvaI    BamHI
    BanII     BbsI     BcgI     BlpI Bme1580I     BplI     BpmI    BsaAI
    BsaBI    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI
 Bsp1286I    BsrBI    BsrDI    BsrGI    BstXI    BstYI   Bsu36I     BtgI
     BtsI   DraIII     DrdI     EaeI     EarI     EciI  Eco57MI EcoO109I
    EcoRI     FalI    HaeII    Hin4I   HincII     MmeI     MscI     MslI
   MspA1I     NspI    PflMI     PfoI     PmlI   PspOMI     PstI    PvuII
     SfcI     StyI    TaqII     TatI  Tth111I     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AlfI     AloI     AscI     AseI    AsiSI    AvrII
     BaeI     BanI     BbeI    BbvCI    BciVI     BclI    BfrBI     BglI
    BglII    BmgBI     BmrI     BmtI   Bpu10I    BpuEI     BsaI    BsaHI
     BsgI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrFI   BssHII
    BssSI   BstAPI    BstBI   BstEII  BstZ17I    BtgZI     ClaI    CspCI
     DraI     EagI  EcoICRI    EcoNI    EcoRV     FseI     FspI    FspAI
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI     PmeI     PpiI    PpuMI    PshAI     PsiI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
     TstI     XbaI     XhoI     XmaI     XmnI     ZraI
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