Restriction map for pF1KB5528
##################################
# NO CUT ENZYMES (  101 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AfaI        AflIII      AgeI        
AhdI        Aor51HI     ApaI        AscI        AseI        
AsnI        Asp718I     AspEI       AvaI        AviII       
AvrII       BanI        BbeI        BbrPI       BglI        
BglII       BlnI        BsaAI       BsaBI       BsaHI       
BseAI       BsiEI       BsiWI       BsoBI       Bsp1407I    
BspEI       BspLU11I    BsrBI       BsrGI       BssHII      
Bst1107I    BstBI       BstEII      BstPI       BstUI       
BstZ17I     CpoI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoO65I     FseI        FspI        
HaeII       HgaI        Hin1I       KasI        KpnI        
KspI        MamI        MfeI        MluI        MroI        
MunI        MvnI        NarI        NdeI        NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PinAI       PmaCI       PmeI        PmlI        PshBI       
Psp1406I    PvuI        RsaI        RsrII       SacII       
SalI        SapI        SbfI        ScaI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
Sse8387I    SspBI       SwaI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AflII         508  CTTAAG               C^TTAAG
AlwNI        3526  CAGAGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I      2498  GAACTAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI           77  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI          24  GGATCC               G^GATCC
BbvCI        2086  GCTGAGG              CCTCAGC(-5/-2)
BciVI        1999  GTATCC               GTATCC(6/5)
BclI         4254  TGATCA               T^GATCA
BfrI          508  CTTAAG               C^TTAAG
BlpI          145  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      145  GCTGAGC              GC^T[ACGT]AGC
BsaWI        2045  ACCGGA               [AT]^CCGG[AT]
BspDI         998  ATCGAT               AT^CGAT
BsrFI        2779  GCCGGC               [GA]^CCGG[CT]
CelII         145  GCTGAGC              GC^T[ACGT]AGC
CfoI          651  GCGC                 GCG^C
Cfr10I       2779  GCCGGC               [GA]^CCGG[CT]
ClaI          998  ATCGAT               AT^CGAT
DraI          213  TTTAAA               TTT^AAA
DraIII       2858  CACTGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          230  GACAGATACGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV         153  GATATC               GAT^ATC
EcoT22I      2017  ATGCAT               ATGCA^T
FauI         4047  CCCGC                CCCGC(4/6)
FbaI         4254  TGATCA               T^GATCA
HhaI          651  GCGC                 GCG^C
HinP1I        651  GCGC                 G^CGC
HincII        457  GTTAAC               GT[CT]^[GA]AC
HindII        457  GTTAAC               GT[CT]^[GA]AC
HindIII       962  AAGCTT               A^AGCTT
HpaI          457  GTTAAC               GTT^AAC
NaeI         2779  GCCGGC               GCC^GGC
NgoMIV       2779  GCCGGC               G^CCGGC
NsiI         2017  ATGCAT               ATGCA^T
PflFI          77  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI        1891  GACCGGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI         3642  GAGCTC               GAGCT^C
SpeI         2422  ACTAGT               A^CTAGT
SspI         1663  AATATT               AAT^ATT
Tth111I        77  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         2397  TCTAGA               T^CTAGA
XmnI         2498  GAACTAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AocI          335  CCTTAGG              CC^T[ACGT]AGG
             2747  CCTCAGG             
BcgI         2535  GCAGTCTATTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             3997  GCAATGATGTCG        
BcnI         1893  CCGGG                CC^[GC]GG
             3485  CCCGG               
BsmBI         108  GAGACG               CGTCTC(1/5)
              389  GAGACG              
BspHI        1439  TCATGA               T^CATGA
             3476  TCATGA              
BsrDI        2879  CATTGC               GCAATG(2/0)
             3997  GCAATG              
BssSI         695  CACGAG               CACGAG(-5/-1)
             3464  CACGAG              
BstXI        2969  CCATGGGAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3197  CCACAGGCCTGG        
Bsu36I        335  CCTTAGG              CC^T[ACGT]AGG
             2747  CCTCAGG             
Eco81I        335  CCTTAGG              CC^T[ACGT]AGG
             2747  CCTCAGG             
MaeII         237  ACGT                 A^CGT
              948  ACGT                
MspA1I       2084  CAGCTG               C[AC]G^C[GT]G
             3166  CAGCTG              
NciI         1893  CCGGG                CC^[GC]GG
             3485  CCCGG               
PflMI        3934  CCAAATTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3964  CCAGCCTTTGG         
PvuII        2084  CAGCTG               CAG^CTG
             3166  CAGCTG              
RcaI         1439  TCATGA               T^CATGA
             3476  TCATGA              
SexAI         452  ACCTGGT              A^CC[AT]GGT
             2904  ACCAGGT             
SphI         1340  GCATGC               GCATG^C
             4238  GCATGC              
Van91I       3934  CCAAATTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3964  CCAGCCTTTGG         
XcmI         1700  CCAAGAGTCCTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2849  CCAGACCTGCACTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   95 enzymes) #
#########################################

AciI        AcsI        AluI        Alw44I      AlwI        
ApaLI       ApoI        AspHI       AvaII       BalI        
BanII       BbsI        BbvI        BfaI        BmyI        
BpmI        BpuAI       BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        Bsp1286I    BspMI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       BstYI       
Cac8I       Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        EcoNI       EcoO109I    
EcoRI       EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HapII       HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspI        MvaI        MwoI        
NcoI        NdeII       NlaIII      NlaIV       NspI        
PleI        PpuMI       PstI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StuI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XhoII       
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