Restriction map for pF1KB5493
 (Linear) (Six-base) MAPSORT of: KIBB5493.seq  Check: 7906  from: 1  to: 2724



KIBB5493.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:27  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     627    1534    2724 
   Size:        627     907    1190

  Fragments arranged by size: 

                1190     907     627
 
Acc65I G'GTAC_C

Cuts at:      0    2203    2724 
   Size:       2203     521
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     158     254     337     809    1600    1840    2724 
   Size:        158      96      83     472     791     240     884

  Fragments arranged by size: 

                 884     791     472     240     158      96      83
 
AfeI AGC'GCT

Cuts at:      0     221    2724 
   Size:        221    2503
 
AleI CACnn'nnGTG

Cuts at:      0     559    1780    2724 
   Size:        559    1221     944

  Fragments arranged by size: 

                1221     944     559
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     702    2724 
   Size:        702    2022
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1102    1134    1943    1975    2724 
   Size:       1102      32     809      32     749

  Fragments arranged by size: 

                1102     809     749      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     132     516    1642    1807    1833    2301    2527    2724 
   Size:        132     384    1126     165      26     468     226     197

  Fragments arranged by size: 

                1126     468     384     226     197     165     132      26
            
 
ApoI r'AATT_y

Cuts at:      0     289    2134    2724 
   Size:        289    1845     590

  Fragments arranged by size: 

                1845     590     289
 
AvaI C'yCGr_G

Cuts at:      0    1001    2724 
   Size:       1001    1723
 
BanI G'GyrC_C

Cuts at:      0      24    1029    2203    2320    2724 
   Size:         24    1005    1174     117     404

  Fragments arranged by size: 

                1174    1005     404     117      24
 
BanII G_rGCy'C

Cuts at:      0     439    1025    1332    1649    2724 
   Size:        439     586     307     317    1075

  Fragments arranged by size: 

                1075     586     439     317     307
 
BbeI G_GCGC'C

Cuts at:      0      28    2724 
   Size:         28    2696
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     842    1886    2584    2724 
   Size:        842    1044     698     140

  Fragments arranged by size: 

                1044     842     698     140
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     192    1647    2115    2724 
   Size:        192    1455     468     609

  Fragments arranged by size: 

                1455     609     468     192
 
BfrBI ATG'CAT

Cuts at:      0     673    2724 
   Size:        673    2051
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1720    2221    2567    2724 
   Size:       1720     501     346     157

  Fragments arranged by size: 

                1720     501     346     157
 
Bme1580I G_kGCm'C

Cuts at:      0     278     756    2323    2724 
   Size:        278     478    1567     401

  Fragments arranged by size: 

                1567     478     401     278
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1693    2605    2724 
   Size:       1693     912     119

  Fragments arranged by size: 

                1693     912     119
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      32     187     244     670     934    1759    2724 
   Size:         32     155      57     426     264     825     965

  Fragments arranged by size: 

                 965     825     426     264     155      57      32
 
Bpu10I CC'TnA_GC

Cuts at:      0     500     722     996    2128    2724 
   Size:        500     222     274    1132     596

  Fragments arranged by size: 

                1132     596     500     274     222
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     403    1633    2724 
   Size:        403    1230    1091

  Fragments arranged by size: 

                1230    1091     403
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1693    2724 
   Size:       1693    1031
 
BsaAI yAC'GTr

Cuts at:      0     717    2724 
   Size:        717    2007
 
BsaBI GATnn'nnATC

Cuts at:      0    2150    2724 
   Size:       2150     574
 
BsaHI Gr'CG_yC

Cuts at:      0      25    2724 
   Size:         25    2699
 
BsaWI w'CCGG_w

Cuts at:      0    1364    2057    2724 
   Size:       1364     693     667

  Fragments arranged by size: 

                1364     693     667
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     361     391     397     427    1102    1132    1285    1315 
   Size:        361      30       6      30     675      30     153      30

Cuts at:   1315    1732    1762    1846    1876    1943    1973    2724 
   Size:        417      30      84      30      67      30     751

  Fragments arranged by size: 

                 751     675     417     361     153      84      67      30
                  30      30      30      30      30      30       6
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      19     949    1110    1293    2005    2518    2668    2724 
   Size:         19     930     161     183     712     513     150      56

  Fragments arranged by size: 

                 930     712     513     183     161     150      56      19
            
 
BseYI C'CCAG_C

Cuts at:      0      38    2724 
   Size:         38    2686
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52    1247    1718    1846    2021    2724 
   Size:         52    1195     471     128     175     703

  Fragments arranged by size: 

                1195     703     471     175     128      52
 
BsiEI CG_ry'CG

Cuts at:      0    2352    2724 
   Size:       2352     372
 
BsiHKAI G_wGCw'C

Cuts at:      0    1332    1649    1944    2724 
   Size:       1332     317     295     780

  Fragments arranged by size: 

                1332     780     317     295
 
BsmI GAATG_Cn'

Cuts at:      0     883    1149    2489    2724 
   Size:        883     266    1340     235

  Fragments arranged by size: 

                1340     883     266     235
 
Bsp1286I G_dGCh'C

Cuts at:      0     278     439     756    1025    1332    1649    1944    2323 
   Size:        278     161     317     269     307     317     295     379

Cuts at:   2323    2724 
   Size:        401

  Fragments arranged by size: 

                 401     379     317     317     307     295     278     269
                 161
 
BspEI T'CCGG_A

Cuts at:      0    1364    2057    2724 
   Size:       1364     693     667

  Fragments arranged by size: 

                1364     693     667
 
BspHI T'CATG_A

Cuts at:      0     465    2142    2724 
   Size:        465    1677     582

  Fragments arranged by size: 

                1677     582     465
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     626     627    1534    2724 
   Size:        626       1     907    1190

  Fragments arranged by size: 

                1190     907     626       1
 
BsrBI CCG'CTC

Cuts at:      0      86      96    2724 
   Size:         86      10    2628

  Fragments arranged by size: 

                2628      86      10
 
BsrDI GCAATG_nn'

Cuts at:      0    1596    2234    2515    2724 
   Size:       1596     638     281     209

  Fragments arranged by size: 

                1596     638     281     209
 
BsrFI r'CCGG_y

Cuts at:      0      27     896    2351    2724 
   Size:         27     869    1455     373

  Fragments arranged by size: 

                1455     869     373      27
 
BsrGI T'GTAC_A

Cuts at:      0     969    2724 
   Size:        969    1755
 
BssSI C'ACGA_G

Cuts at:      0     175    2724 
   Size:        175    2549
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1833    2724 
   Size:       1833     891
 
BstBI TT'CG_AA

Cuts at:      0    2418    2724 
   Size:       2418     306
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1779    2190    2724 
   Size:       1779     411     534

  Fragments arranged by size: 

                1779     534     411
 
BtgI C'CryG_G

Cuts at:      0    1212    2183    2375    2625    2724 
   Size:       1212     971     192     250      99

  Fragments arranged by size: 

                1212     971     250     192      99
 
BtsI GCAGTG_nn'

Cuts at:      0    1086    1390    1495    2205    2552    2724 
   Size:       1086     304     105     710     347     172

  Fragments arranged by size: 

                1086     710     347     304     172     105
 
EaeI y'GGCC_r

Cuts at:      0     386    1155    2025    2372    2512    2645    2724 
   Size:        386     769     870     347     140     133      79

  Fragments arranged by size: 

                 870     769     386     347     140     133      79
 
EarI CTCTTCn'nnn_

Cuts at:      0     134    1337    2281    2724 
   Size:        134    1203     944     443

  Fragments arranged by size: 

                1203     944     443     134
 
EcoICRI GAG'CTC

Cuts at:      0    1330    1647    2724 
   Size:       1330     317    1077

  Fragments arranged by size: 

                1330    1077     317
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      32     158     187     244     254     337     670     809 
   Size:         32     126      29      57      10      83     333     139

Cuts at:    809     934    1600    1759    1840    2724 
   Size:        125     666     159      81     884

  Fragments arranged by size: 

                 884     666     333     159     139     126     125      83
                  81      57      32      29      10
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1702    2724 
   Size:       1702    1022
 
EcoO109I rG'GnC_Cy

Cuts at:      0     945    1583    2262    2298    2724 
   Size:        945     638     679      36     426

  Fragments arranged by size: 

                 945     679     638     426      36
 
HaeII r_GCGC'y

Cuts at:      0      28     223    2724 
   Size:         28     195    2501

  Fragments arranged by size: 

                2501     195      28
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     361     393     946     978    1510    1542    1673    1705 
   Size:        361      32     553      32     532      32     131      32

Cuts at:   1705    1732    1764    2099    2131    2724 
   Size:         27      32     335      32     593

  Fragments arranged by size: 

                 593     553     532     361     335     131      32      32
                  32      32      32      32      27
 
KasI G'GCGC_C

Cuts at:      0      24    2724 
   Size:         24    2700
 
KpnI G_GTAC'C

Cuts at:      0    2207    2724 
   Size:       2207     517
 
MscI TGG'CCA

Cuts at:      0     388    1157    2027    2647    2724 
   Size:        388     769     870     620      77

  Fragments arranged by size: 

                 870     769     620     388      77
 
MslI CAynn'nnrTG

Cuts at:      0     394     470     559    1537    1780    2188    2724 
   Size:        394      76      89     978     243     408     536

  Fragments arranged by size: 

                 978     536     408     394     243      89      76
 
MspA1I CmG'CkG

Cuts at:      0      52     540    1231    1833    2724 
   Size:         52     488     691     602     891

  Fragments arranged by size: 

                 891     691     602     488      52
 
NaeI GCC'GGC

Cuts at:      0     898    2724 
   Size:        898    1826
 
NarI GG'CG_CC

Cuts at:      0      25    2724 
   Size:         25    2699
 
NcoI C'CATG_G

Cuts at:      0    2183    2724 
   Size:       2183     541
 
NdeI CA'TA_TG

Cuts at:      0     675    1797    2724 
   Size:        675    1122     927

  Fragments arranged by size: 

                1122     927     675
 
NgoMIV G'CCGG_C

Cuts at:      0     896    2724 
   Size:        896    1828
 
NsiI A_TGCA'T

Cuts at:      0     675    2724 
   Size:        675    2049
 
NspI r_CATG'y

Cuts at:      0     648    2724 
   Size:        648    2076
 
PasI CC'CwG_GG

Cuts at:      0     343     756    2724 
   Size:        343     413    1968

  Fragments arranged by size: 

                1968     413     343
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     307    2547    2724 
   Size:        307    2240     177

  Fragments arranged by size: 

                2240     307     177
 
PfoI T'CCnGG_A

Cuts at:      0     911    2117    2408    2724 
   Size:        911    1206     291     316

  Fragments arranged by size: 

                1206     911     316     291
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1102    1134    1422    1454    1943    1975    2724 
   Size:       1102      32     288      32     489      32     749

  Fragments arranged by size: 

                1102     749     489     288      32      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     945    1583    2262    2724 
   Size:        945     638     679     462

  Fragments arranged by size: 

                 945     679     638     462
 
PvuII CAG'CTG

Cuts at:      0    1231    1833    2724 
   Size:       1231     602     891

  Fragments arranged by size: 

                1231     891     602
 
SacI G_AGCT'C

Cuts at:      0    1332    1649    2724 
   Size:       1332     317    1075

  Fragments arranged by size: 

                1332    1075     317
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1337    2724 
   Size:       1337    1387
 
SfoI GGC'GCC

Cuts at:      0      26    2724 
   Size:         26    2698
 
SgrAI Cr'CCGG_yG

Cuts at:      0      27    2724 
   Size:         27    2697
 
SmlI C'TyrA_G

Cuts at:      0     418    1648    2724 
   Size:        418    1230    1076

  Fragments arranged by size: 

                1230    1076     418
 
StyI C'CwwG_G

Cuts at:      0    1025    2183    2258    2724 
   Size:       1025    1158      75     466

  Fragments arranged by size: 

                1158    1025     466      75
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     748    1294    2724 
   Size:        748     546    1430

  Fragments arranged by size: 

                1430     748     546
 
TatI w'GTAC_w

Cuts at:      0     867     969    1298    2724 
   Size:        867     102     329    1426

  Fragments arranged by size: 

                1426     867     329     102
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     397     429    1359    1391    1846    1878    2724 
   Size:        397      32     930      32     455      32     846

  Fragments arranged by size: 

                 930     846     455     397      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1685    2724 
   Size:       1685    1039
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1536    1779    2181    2190    2724 
   Size:       1536     243     402       9     534

  Fragments arranged by size: 

                1536     534     402     243       9
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI     AfeI     AleI     AlfI     AloI    AlwNI
     ApoI     AvaI     BanI    BanII     BbeI     BbsI    BciVI    BfrBI
     BglI Bme1580I     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI    BssSI   BstAPI    BstBI    BstXI     BtgI     BtsI
     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I
     KasI     KpnI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NsiI     NspI     PasI    PflMI     PfoI     PpiI
    PpuMI    PvuII     SacI     SapI     SfoI    SgrAI     SmlI     StyI
    TaqII     TatI     TstI  Tth111I     XcmI

 Enzymes that do not cut: 

    AatII     AccI     AclI    AflII   AflIII     AgeI     AhdI     ApaI
    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BbvCI
     BcgI     BclI    BglII     BlpI    BmgBI     BmtI     BplI    BsiWI
    BsmBI   BssHII   BstEII    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EciI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI     MfeI
     MluI     MmeI     NheI     NotI     NruI     PacI     PciI     PmeI
     PmlI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfcI
     SfiI     SgfI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     XbaI     XhoI     XmaI     XmnI     ZraI
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