Restriction map for pF1KB5485
 (Linear) (Six-base) MAPSORT of: KIBB5485.seq  Check: 7475  from: 1  to: 1551



KIBB5485.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:27  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1071    1551 
   Size:       1071     480
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     227     486    1294    1529    1551 
   Size:        227     259     808     235      22

  Fragments arranged by size: 

                 808     259     235     227      22
 
AleI CACnn'nnGTG

Cuts at:      0    1510    1551 
   Size:       1510      41
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1272    1551 
   Size:       1272     279
 
ApaI G_GGCC'C

Cuts at:      0      29     592    1551 
   Size:         29     563     959

  Fragments arranged by size: 

                 959     563      29
 
ApoI r'AATT_y

Cuts at:      0     555    1551 
   Size:        555     996
 
AvaI C'yCGr_G

Cuts at:      0      94     248    1022    1034    1551 
   Size:         94     154     774      12     517

  Fragments arranged by size: 

                 774     517     154      94      12
 
BanI G'GyrC_C

Cuts at:      0     278    1551 
   Size:        278    1273
 
BanII G_rGCy'C

Cuts at:      0      29     271     592     871    1434    1551 
   Size:         29     242     321     279     563     117

  Fragments arranged by size: 

                 563     321     279     242     117      29
 
BbeI G_GCGC'C

Cuts at:      0     282    1551 
   Size:        282    1269
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      93    1335    1551 
   Size:         93    1242     216

  Fragments arranged by size: 

                1242     216      93
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1215    1249    1551 
   Size:       1215      34     302

  Fragments arranged by size: 

                1215     302      34
 
BclI T'GATC_A

Cuts at:      0     671    1551 
   Size:        671     880
 
BglI GCCn_nnn'nGGC

Cuts at:      0      67     250     454    1390    1551 
   Size:         67     183     204     936     161

  Fragments arranged by size: 

                 936     204     183     161      67
 
BglII A'GATC_T

Cuts at:      0    1247    1551 
   Size:       1247     304
 
BlpI GC'TnA_GC

Cuts at:      0     632    1551 
   Size:        632     919
 
Bme1580I G_kGCm'C

Cuts at:      0      29     592    1399    1551 
   Size:         29     563     807     152

  Fragments arranged by size: 

                 807     563     152      29
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1428    1551 
   Size:       1428     123
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     203    1372    1551 
   Size:        203    1169     179

  Fragments arranged by size: 

                1169     203     179
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     820    1551 
   Size:        820     731
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     361    1551 
   Size:        361    1190
 
BsaAI yAC'GTr

Cuts at:      0    1322    1551 
   Size:       1322     229
 
BsaBI GATnn'nnATC

Cuts at:      0    1267    1551 
   Size:       1267     284
 
BsaHI Gr'CG_yC

Cuts at:      0     279    1551 
   Size:        279    1272
 
BsaWI w'CCGG_w

Cuts at:      0     344     437    1551 
   Size:        344      93    1114

  Fragments arranged by size: 

                1114     344      93
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1258    1306    1551 
   Size:       1258      48     245

  Fragments arranged by size: 

                1258     245      48
 
BseYI C'CCAG_C

Cuts at:      0     264     452     585     591     733     820     855    1551 
   Size:        264     188     133       6     142      87      35     696

  Fragments arranged by size: 

                 696     264     188     142     133      87      35       6
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1000    1237    1551 
   Size:       1000     237     314

  Fragments arranged by size: 

                1000     314     237
 
BsiWI C'GTAC_G

Cuts at:      0    1322    1551 
   Size:       1322     229
 
BsmI GAATG_Cn'

Cuts at:      0     775    1551 
   Size:        775     776
 
Bsp1286I G_dGCh'C

Cuts at:      0      29     271     592     871    1399    1434    1551 
   Size:         29     242     321     279     528      35     117

  Fragments arranged by size: 

                 528     321     279     242     117      35      29
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1071    1551 
   Size:       1071     480
 
BsrBI CCG'CTC

Cuts at:      0     339     564    1011    1551 
   Size:        339     225     447     540

  Fragments arranged by size: 

                 540     447     339     225
 
BsrDI GCAATG_nn'

Cuts at:      0     743    1166    1551 
   Size:        743     423     385

  Fragments arranged by size: 

                 743     423     385
 
BsrFI r'CCGG_y

Cuts at:      0     305     448     961    1551 
   Size:        305     143     513     590

  Fragments arranged by size: 

                 590     513     305     143
 
BssHII G'CGCG_C

Cuts at:      0       6    1551 
   Size:          6    1545
 
BstYI r'GATC_y

Cuts at:      0    1106    1247    1268    1551 
   Size:       1106     141      21     283

  Fragments arranged by size: 

                1106     283     141      21
 
BtgI C'CryG_G

Cuts at:      0     468     550     728     908    1179    1551 
   Size:        468      82     178     180     271     372

  Fragments arranged by size: 

                 468     372     271     180     178      82
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1218    1551 
   Size:       1218     333
 
BtsI GCAGTG_nn'

Cuts at:      0     242    1551 
   Size:        242    1309
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     777     812    1188    1223    1551 
   Size:        777      35     376      35     328

  Fragments arranged by size: 

                 777     376     328      35      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    1403    1551 
   Size:       1403     148
 
EaeI y'GGCC_r

Cuts at:      0     323     662     798    1331    1470    1551 
   Size:        323     339     136     533     139      81

  Fragments arranged by size: 

                 533     339     323     139     136      81
 
EarI CTCTTCn'nnn_

Cuts at:      0     859    1551 
   Size:        859     692
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     203     227     486    1294    1372    1529    1551 
   Size:        203      24     259     808      78     157      22

  Fragments arranged by size: 

                 808     259     203     157      78      24      22
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     673    1378    1551 
   Size:        673     705     173

  Fragments arranged by size: 

                 705     673     173
 
EcoO109I rG'GnC_Cy

Cuts at:      0      26    1383    1551 
   Size:         26    1357     168

  Fragments arranged by size: 

                1357     168      26
 
HaeII r_GCGC'y

Cuts at:      0     282    1551 
   Size:        282    1269
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     176     208    1551 
   Size:        176      32    1343

  Fragments arranged by size: 

                1343     176      32
 
HincII GTy'rAC

Cuts at:      0     705    1410    1551 
   Size:        705     705     141

  Fragments arranged by size: 

                 705     705     141
 
KasI G'GCGC_C

Cuts at:      0     278    1551 
   Size:        278    1273
 
MscI TGG'CCA

Cuts at:      0     664    1551 
   Size:        664     887
 
MslI CAynn'nnrTG

Cuts at:      0     758     880    1510    1551 
   Size:        758     122     630      41

  Fragments arranged by size: 

                 758     630     122      41
 
MspA1I CmG'CkG

Cuts at:      0     264    1339    1551 
   Size:        264    1075     212

  Fragments arranged by size: 

                1075     264     212
 
NaeI GCC'GGC

Cuts at:      0     307     450     963    1551 
   Size:        307     143     513     588

  Fragments arranged by size: 

                 588     513     307     143
 
NarI GG'CG_CC

Cuts at:      0     279    1551 
   Size:        279    1272
 
NgoMIV G'CCGG_C

Cuts at:      0     305     448     961    1551 
   Size:        305     143     513     590

  Fragments arranged by size: 

                 590     513     305     143
 
PfoI T'CCnGG_A

Cuts at:      0     967    1551 
   Size:        967     584
 
PspOMI G'GGCC_C

Cuts at:      0      25     588    1551 
   Size:         25     563     963

  Fragments arranged by size: 

                 963     563      25
 
PstI C_TGCA'G

Cuts at:      0    1499    1551 
   Size:       1499      52
 
PvuII CAG'CTG

Cuts at:      0     264    1339    1551 
   Size:        264    1075     212

  Fragments arranged by size: 

                1075     264     212
 
SapI GCTCTTCn'nnn_

Cuts at:      0     859    1551 
   Size:        859     692
 
SexAI A'CCwGG_T

Cuts at:      0     404    1551 
   Size:        404    1147
 
SfcI C'TryA_G

Cuts at:      0    1495    1551 
   Size:       1495      56
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     250    1551 
   Size:        250    1301
 
SfoI GGC'GCC

Cuts at:      0     280    1551 
   Size:        280    1271
 
SmaI CCC'GGG

Cuts at:      0     250    1024    1551 
   Size:        250     774     527

  Fragments arranged by size: 

                 774     527     250
 
SmlI C'TyrA_G

Cuts at:      0     835    1551 
   Size:        835     716
 
SrfI GCCC'GGGC

Cuts at:      0     250    1024    1551 
   Size:        250     774     527

  Fragments arranged by size: 

                 774     527     250
 
StyI C'CwwG_G

Cuts at:      0     274     362     596     848    1238    1551 
   Size:        274      88     234     252     390     313

  Fragments arranged by size: 

                 390     313     274     252     234      88
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      79    1081    1551 
   Size:         79    1002     470

  Fragments arranged by size: 

                1002     470      79
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     156     188    1551 
   Size:        156      32    1363

  Fragments arranged by size: 

                1363     156      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     421    1551 
   Size:        421    1130
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     817    1035    1326    1441    1551 
   Size:        817     218     291     115     110

  Fragments arranged by size: 

                 817     291     218     115     110
 
XmaI C'CCGG_G

Cuts at:      0     248    1022    1551 
   Size:        248     774     529

  Fragments arranged by size: 

                 774     529     248
 
XmnI GAAnn'nnTTC

Cuts at:      0     157    1551 
   Size:        157    1394
 

 Enzymes that do cut:

     AarI     AcuI     AleI    AlwNI     ApaI     ApoI     AvaI     BanI
    BanII     BbeI     BbsI     BcgI     BclI     BglI    BglII     BlpI
 Bme1580I     BmrI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseRI    BseYI     BsgI    BsiWI     BsmI Bsp1286I    BspMI
    BsrBI    BsrDI    BsrFI   BssHII    BstYI     BtgI    BtgZI     BtsI
    CspCI   DraIII     EaeI     EarI  Eco57MI    EcoNI EcoO109I    HaeII
    Hin4I   HincII     KasI     MscI     MslI   MspA1I     NaeI     NarI
   NgoMIV     PfoI   PspOMI     PstI    PvuII     SapI    SexAI     SfcI
     SfiI     SfoI     SmaI     SmlI     SrfI     StyI    TaqII     TstI
  Tth111I     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI    BbvCI    BciVI    BfrBI    BmgBI     BmtI     BplI
   Bpu10I    BsaXI    BsiEI  BsiHKAI    BsmBI    BspEI    BspHI    BsrGI
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     ClaI
     DraI     DrdI     EagI     EciI  EcoICRI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI  HindIII     HpaI     KpnI     MfeI     MluI
     MmeI     NcoI     NdeI     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SbfI     ScaI     SgfI    SgrAI    SnaBI     SpeI
     SphI     SspI     StuI     SwaI     TatI     XbaI     XhoI     ZraI
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