Restriction map for pF1KB5456
 (Linear) (Six-base) MAPSORT of: KIBB5456.seq  Check: 1645  from: 1  to: 1257



KIBB5456.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:27  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     128     582     681    1257 
   Size:        128     454      99     576

  Fragments arranged by size: 

                 576     454     128      99
 
AatII G_ACGT'C

Cuts at:      0     442    1257 
   Size:        442     815
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     110     361     403     871     922    1048    1257 
   Size:        110     251      42     468      51     126     209

  Fragments arranged by size: 

                 468     251     209     126     110      51      42
 
AfeI AGC'GCT

Cuts at:      0     157     223    1257 
   Size:        157      66    1034

  Fragments arranged by size: 

                1034     157      66
 
AflIII A'CryG_T

Cuts at:      0    1236    1257 
   Size:       1236      21
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     256     288    1257 
   Size:        256      32     969

  Fragments arranged by size: 

                 969     256      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     213     363     507    1257 
   Size:        213     150     144     750

  Fragments arranged by size: 

                 750     213     150     144
 
ApaI G_GGCC'C

Cuts at:      0     522    1257 
   Size:        522     735
 
AvaI C'yCGr_G

Cuts at:      0     448     521     953    1165    1257 
   Size:        448      73     432     212      92

  Fragments arranged by size: 

                 448     432     212      92      73
 
BanI G'GyrC_C

Cuts at:      0       8    1257 
   Size:          8    1249
 
BanII G_rGCy'C

Cuts at:      0     182     522     983    1162    1257 
   Size:        182     340     461     179      95

  Fragments arranged by size: 

                 461     340     182     179      95
 
BbvCI CC'TCA_GC

Cuts at:      0     407    1257 
   Size:        407     850
 
BglI GCCn_nnn'nGGC

Cuts at:      0     526     814     823    1257 
   Size:        526     288       9     434

  Fragments arranged by size: 

                 526     434     288       9
 
BglII A'GATC_T

Cuts at:      0     783    1257 
   Size:        783     474
 
Bme1580I G_kGCm'C

Cuts at:      0     522    1257 
   Size:        522     735
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      41      88     678    1257 
   Size:         41      47     590     579

  Fragments arranged by size: 

                 590     579      47      41
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     136     184     346     529     571     784    1018    1257 
   Size:        136      48     162     183      42     213     234     239

  Fragments arranged by size: 

                 239     234     213     183     162     136      48      42
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     407    1257 
   Size:        407     850
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     139     880    1092    1257 
   Size:        139     741     212     165

  Fragments arranged by size: 

                 741     212     165     139
 
BsaHI Gr'CG_yC

Cuts at:      0     439    1257 
   Size:        439     818
 
BsaWI w'CCGG_w

Cuts at:      0     894    1257 
   Size:        894     363
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     256     286     782     812    1257 
   Size:        256      30     496      30     445

  Fragments arranged by size: 

                 496     445     256      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      83     181     244     277     679     826     871    1116 
   Size:         83      98      63      33     402     147      45     245

Cuts at:   1116    1167    1170    1183    1257 
   Size:         51       3      13      74

  Fragments arranged by size: 

                 402     245     147      98      83      74      63      51
                  45      33      13       3
 
BseYI C'CCAG_C

Cuts at:      0    1144    1257 
   Size:       1144     113
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      61     100     610    1257 
   Size:         61      39     510     647

  Fragments arranged by size: 

                 647     510      61      39
 
BsiHKAI G_wGCw'C

Cuts at:      0     182     380     983    1257 
   Size:        182     198     603     274

  Fragments arranged by size: 

                 603     274     198     182
 
BsmI GAATG_Cn'

Cuts at:      0     502     776    1257 
   Size:        502     274     481

  Fragments arranged by size: 

                 502     481     274
 
Bsp1286I G_dGCh'C

Cuts at:      0     182     380     522     983    1162    1257 
   Size:        182     198     142     461     179      95

  Fragments arranged by size: 

                 461     198     182     179     142      95
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     128     582     681    1257 
   Size:        128     454      99     576

  Fragments arranged by size: 

                 576     454     128      99
 
BsrBI CCG'CTC

Cuts at:      0     759    1257 
   Size:        759     498
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     363     588    1257 
   Size:        363     225     669

  Fragments arranged by size: 

                 669     363     225
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     306    1257 
   Size:        306     951
 
BstYI r'GATC_y

Cuts at:      0     783    1257 
   Size:        783     474
 
BtsI GCAGTG_nn'

Cuts at:      0     759    1257 
   Size:        759     498
 
ClaI AT'CG_AT

Cuts at:      0     842    1257 
   Size:        842     415
 
DraIII CAC_nnn'GTG

Cuts at:      0     588    1257 
   Size:        588     669
 
EaeI y'GGCC_r

Cuts at:      0     527     824     965    1049    1257 
   Size:        527     297     141      84     208

  Fragments arranged by size: 

                 527     297     208     141      84
 
EarI CTCTTCn'nnn_

Cuts at:      0      13      80     337     379     664     715    1257 
   Size:         13      67     257      42     285      51     542

  Fragments arranged by size: 

                 542     285     257      67      51      42      13
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     928     943    1257 
   Size:        928      15     314

  Fragments arranged by size: 

                 928     314      15
 
EcoICRI GAG'CTC

Cuts at:      0     180     981    1257 
   Size:        180     801     276

  Fragments arranged by size: 

                 801     276     180
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     110     136     184     346     361     403     529     571 
   Size:        110      26      48     162      15      42     126      42

Cuts at:    571     784     871     922    1018    1048    1257 
   Size:        213      87      51      96      30     209

  Fragments arranged by size: 

                 213     209     162     126     110      96      87      51
                  48      42      42      30      26      15
 
EcoO109I rG'GnC_Cy

Cuts at:      0     816    1095    1209    1257 
   Size:        816     279     114      48

  Fragments arranged by size: 

                 816     279     114      48
 
EcoRV GAT'ATC

Cuts at:      0     918    1257 
   Size:        918     339
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     948    1257 
   Size:        948     309
 
FspI TGC'GCA

Cuts at:      0     607    1257 
   Size:        607     650
 
HaeII r_GCGC'y

Cuts at:      0     140     159     225     571     651    1257 
   Size:        140      19      66     346      80     606

  Fragments arranged by size: 

                 606     346     140      80      66      19
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     176     208     403     435     456     488    1257 
   Size:        176      32     195      32      21      32     769

  Fragments arranged by size: 

                 769     195     176      32      32      32      21
 
MscI TGG'CCA

Cuts at:      0     826    1257 
   Size:        826     431
 
MspA1I CmG'CkG

Cuts at:      0     549     603     777     996    1005    1037    1257 
   Size:        549      54     174     219       9      32     220

  Fragments arranged by size: 

                 549     220     219     174      54      32       9
 
NspI r_CATG'y

Cuts at:      0     612    1081    1240    1257 
   Size:        612     469     159      17

  Fragments arranged by size: 

                 612     469     159      17
 
PciI A'CATG_T

Cuts at:      0    1236    1257 
   Size:       1236      21
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     507     624    1257 
   Size:        507     117     633

  Fragments arranged by size: 

                 633     507     117
 
PfoI T'CCnGG_A

Cuts at:      0     290    1257 
   Size:        290     967
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     256     288    1257 
   Size:        256      32     969

  Fragments arranged by size: 

                 969     256      32
 
PspOMI G'GGCC_C

Cuts at:      0     518    1257 
   Size:        518     739
 
PstI C_TGCA'G

Cuts at:      0     461     575     989    1010    1232    1257 
   Size:        461     114     414      21     222      25

  Fragments arranged by size: 

                 461     414     222     114      25      21
 
PvuII CAG'CTG

Cuts at:      0     549     603     777     996    1005    1037    1257 
   Size:        549      54     174     219       9      32     220

  Fragments arranged by size: 

                 549     220     219     174      54      32       9
 
SacI G_AGCT'C

Cuts at:      0     182     983    1257 
   Size:        182     801     274

  Fragments arranged by size: 

                 801     274     182
 
SapI GCTCTTCn'nnn_

Cuts at:      0      13     715    1257 
   Size:         13     702     542

  Fragments arranged by size: 

                 702     542      13
 
SbfI CC_TGCA'GG

Cuts at:      0     989    1257 
   Size:        989     268
 
SfcI C'TryA_G

Cuts at:      0     457     571     985    1006    1228    1257 
   Size:        457     114     414      21     222      29

  Fragments arranged by size: 

                 457     414     222     114      29      21
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     526     814     823    1257 
   Size:        526     288       9     434

  Fragments arranged by size: 

                 526     434     288       9
 
SmaI CCC'GGG

Cuts at:      0     523     955    1257 
   Size:        523     432     302

  Fragments arranged by size: 

                 523     432     302
 
SphI G_CATG'C

Cuts at:      0     612    1257 
   Size:        612     645
 
SrfI GCCC'GGGC

Cuts at:      0     523    1257 
   Size:        523     734
 
StuI AGG'CCT

Cuts at:      0     418    1257 
   Size:        418     839
 
TatI w'GTAC_w

Cuts at:      0    1016    1257 
   Size:       1016     241
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     306    1257 
   Size:        306     951
 
XmaI C'CCGG_G

Cuts at:      0     521     953    1257 
   Size:        521     432     304

  Fragments arranged by size: 

                 521     432     304
 
XmnI GAAnn'nnTTC

Cuts at:      0     271    1257 
   Size:        271     986
 
ZraI GAC'GTC

Cuts at:      0     440    1257 
   Size:        440     817
 

 Enzymes that do cut:

     AarI    AatII     AcuI     AfeI   AflIII     AloI    AlwNI     ApaI
     AvaI     BanI    BanII    BbvCI     BglI    BglII Bme1580I     BmrI
     BpmI   Bpu10I     BsaI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrBI   BstAPI    BstXI
    BstYI     BtsI     ClaI   DraIII     EaeI     EarI     EciI  EcoICRI
  Eco57MI EcoO109I    EcoRV     FalI     FspI    HaeII    Hin4I     MscI
   MspA1I     NspI     PciI    PflMI     PfoI     PpiI   PspOMI     PstI
    PvuII     SacI     SapI     SbfI     SfcI     SfiI     SmaI     SphI
     SrfI     StuI     TatI     XcmI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI    AflII     AgeI     AhdI     AleI     AlfI
    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI     BlpI    BmgBI
     BmtI     BplI    BpuEI    BsaAI    BsaBI    BsiEI    BsiWI    BsmBI
    BspEI    BspHI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI
   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI    CspCI     DraI     DrdI
     EagI    EcoNI    EcoRI     FseI    FspAI   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MslI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PmeI     PmlI    PpuMI    PshAI     PsiI    PspXI     PsrI
     PvuI    RsrII    SacII     SalI    SanDI     ScaI    SexAI     SfoI
     SgfI    SgrAI     SmlI    SnaBI     SpeI     SspI     StyI     SwaI
    TaqII     TstI  Tth111I     XbaI     XhoI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com