Restriction map for pF1KB5448
##################################
# NO CUT ENZYMES (  176 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvaI        
AviII       AvrII       BalI        BamHI       BanII       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BcnI        BfrI        BglI        BglII       
BlnI        BlpI        BpmI        Bpu1102I    BsaAI       
BsaHI       BsaI        BsaJI       BseRI       BsgI        
BsiEI       BsiWI       BsmAI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspDI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstZ17I     CelII       ClaI        CpoI        
DraI        DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      EcoO65I     EcoRI       EcoT14I     EcoT22I     
FbaI        FseI        FspI        HgaI        Hin1I       
HincII      HindII      HindIII     HinfI       HpaI        
KasI        KpnI        Ksp632I     KspI        MaeIII      
MfeI        MluI        MluNI       MscI        MseI        
MslI        MunI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfaNI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        TfiI        
Tru9I       Tsp45I      Tsp509I     Tth111I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccIII         11  TCCGGA               T^CCGGA
AciI          384  GCGG                 CCGC(-3/-1)
AfaI          465  GTAC                 GT^AC
AlwI          357  GGATC                GGATC(4/5)
AocI          204  CCTGAGG              CC^T[ACGT]AGG
AspHI         373  GAGCAC               G[AT]GC[AT]^C
BanI          566  GGTGCC               G^G[CT][GA]CC
BbsI           70  GTCTTC               GAAGAC(2/6)
BpuAI          70  GTCTTC               GAAGAC(2/6)
BsaBI         193  GATTATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI          11  TCCGGA               [AT]^CCGG[AT]
BseAI          11  TCCGGA               T^CCGGA
BsiHKAI       373  GAGCAC               G[AT]GC[AT]^C
BsiYI         421  CCTGAAGAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          421  CCTGAAGAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspEI          11  TCCGGA               T^CCGGA
BspMI         153  GCAGGT               ACCTGC(4/8)
BsrFI         411  GCCGGT               [GA]^CCGG[CT]
BssKI         339  CCTGG                ^CC[ACGT]GG
BstNI         339  CCTGG                CC^[AT]GG
BstYI         357  GGATCT               [GA]^GATC[CT]
Bsu36I        204  CCTGAGG              CC^T[ACGT]AGG
CfoI          455  GCGC                 GCG^C
Cfr10I        411  GCCGGT               [GA]^CCGG[CT]
DdeI          205  CTGAG                C^T[ACGT]AG
DraII         208  AGGCCCC              [GA]G^G[ACGT]CC[CT]
Eco81I        204  CCTGAGG              CC^T[ACGT]AGG
EcoNI         421  CCTGAAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      208  AGGCCCC              [GA]G^G[ACGT]CC[CT]
EcoRII        339  CCTGG                ^CC[AT]GG
EcoRV         133  GATATC               GAT^ATC
FauI          384  GCGGG                CCCGC(4/6)
HaeII         454  GGCGCT               [GA]GCGC^[CT]
HaeIII        209  GGCC                 GG^CC
HhaI          455  GCGC                 GCG^C
HinP1I        455  GCGC                 G^CGC
MamI          193  GATTATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          357  GGATCT               [GA]^GATC[CT]
MroI           11  TCCGGA               T^CCGGA
MspA1I        179  CAGCTG               C[AC]G^C[GT]G
MvaI          339  CCTGG                CC^[AT]GG
NheI          390  GCTAGC               G^CTAGC
NspI           55  ACATGC               [GA]CATG^[CT]
PvuII         179  CAGCTG               CAG^CTG
RsaI          465  GTAC                 GT^AC
ScrFI         339  CCTGG                CC^[ACGT]GG
TspRI         110  CAGTG                CA[GC]TG[ACGT][ACGT]^
XhoII         357  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AvaII          81  GGACC                G^G[AT]CC
              296  GGACC               
BbvI            4  GCTGC                GCAGC(8/12)
              529  GCTGC               
BfaI          391  CTAG                 C^TAG
              542  CTAG                
BsmFI         173  GGGAC                GGGAC(10/14)
              320  GTCCC               
BsrI           21  ACTGG                ACTGG(1/-1)
               84  CCAGT               
BstXI         286  CCAGAAAAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              332  CCATCATCCTGG        
DpnII         199  GATC                 ^GATC
              358  GATC                
Fnu4HI          4  GCTGC                GC^[ACGT]GC
              529  GCTGC               
HapII          12  CCGG                 C^CGG
              412  CCGG                
HpaII          12  CCGG                 C^CGG
              412  CCGG                
ItaI            4  GCTGC                GC^[ACGT]GC
              529  GCTGC               
MaeI          391  CTAG                 C^TAG
              542  CTAG                
MaeII         326  ACGT                 A^CGT
              546  ACGT                
MboI          199  GATC                 ^GATC
              358  GATC                
MspI           12  CCGG                 C^CGG
              412  CCGG                
NdeII         199  GATC                 ^GATC
              358  GATC                
NlaIII         56  CATG                 CATG^
              468  CATG                
Sau3AI        199  GATC                 ^GATC
              358  GATC                
TaqI          137  TCGA                 T^CGA
              257  TCGA                
TseI            4  GCTGC                G^C[AT]GC
              529  GCTGC               
TthHB8I       137  TCGA                 T^CGA
              257  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   13 enzymes) #
#########################################

AluI        BmyI        Bsp1286I    BstF5I      Cac8I       
Cfr13I      FokI        HphI        MboII       MnlI        
MwoI        NlaIV       Sau96I      
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