Restriction map for
pF1KB5439
(Circular) (Six-base) MAPSORT of: pF1KB5439.seq Check: 6472 from: 1 to: 3734
pF1KB5439 3734 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 772 772
Size: 3734
Acc65I G'GTAC_C
Cuts at: 750 750
Size: 3734
AclI AA'CG_TT
Cuts at: 3574 3574
Size: 3734
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 1559 1991 2344 3006 154
Size: 1405 432 353 662 882
Fragments arranged by size:
1405 882 662 432 353
AflII C'TTAA_G
Cuts at: 628 628
Size: 3734
AflIII A'CryG_T
Cuts at: 1086 2886 3167 1086
Size: 1800 281 1653
Fragments arranged by size:
1800 1653 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 372 735 372
Size: 363 3371
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 586 618 586
Size: 32 3702
AlwNI CAG_nnn'CTG
Cuts at: 1039 2477 1039
Size: 1438 2296
ApaLI G'TGCA_C
Cuts at: 2572 2572
Size: 3734
ApoI r'AATT_y
Cuts at: 202 661 738 3087 3343 202
Size: 459 77 2349 256 593
Fragments arranged by size:
2349 593 459 256 77
AseI AT'TA_AT
Cuts at: 20 20
Size: 3734
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3734
AvaI C'yCGr_G
Cuts at: 443 754 443
Size: 311 3423
BamHI G'GATC_C
Cuts at: 759 759
Size: 3734
BanI G'GyrC_C
Cuts at: 750 1164 1412 1447 750
Size: 414 248 35 3037
Fragments arranged by size:
3037 414 248 35
BanII G_rGCy'C
Cuts at: 748 1778 748
Size: 1030 2704
BbeI G_GCGC'C
Cuts at: 1168 1416 1168
Size: 248 3486
BbsI GAAGACnn'nnnn_
Cuts at: 175 175
Size: 3734
BciVI GTATCCnnnnn_n'
Cuts at: 1632 2688 3734 1632
Size: 1056 1046 1632
Fragments arranged by size:
1632 1056 1046
BclI T'GATC_A
Cuts at: 1254 1254
Size: 3734
BglI GCCn_nnn'nGGC
Cuts at: 580 580
Size: 3734
BglII A'GATC_T
Cuts at: 1249 3286 1249
Size: 2037 1697
BlpI GC'TnA_GC
Cuts at: 846 846
Size: 3734
Bme1580I G_kGCm'C
Cuts at: 525 1359 1452 2576 525
Size: 834 93 1124 1683
Fragments arranged by size:
1683 1124 834 93
BmrI ACTGGGnnnn_n'
Cuts at: 946 1027 1362 3506 946
Size: 81 335 2144 1174
Fragments arranged by size:
2144 1174 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2101 2101
Size: 3734
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 179 432 179
Size: 253 3481
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 910 1789 2503 2801 910
Size: 879 714 298 1843
Fragments arranged by size:
1843 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 559 3438 559
Size: 2879 855
BsaAI yAC'GTr
Cuts at: 1087 1087
Size: 3734
BsaBI GATnn'nnATC
Cuts at: 6 1248 3285 6
Size: 1242 2037 455
Fragments arranged by size:
2037 1242 455
BsaHI Gr'CG_yC
Cuts at: 1165 1413 2115 3611 1165
Size: 248 702 1496 1288
Fragments arranged by size:
1496 1288 702 248
BsaWI w'CCGG_w
Cuts at: 923 1444 2122 2533 2680 2954 923
Size: 521 678 411 147 274 1703
Fragments arranged by size:
1703 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 586 616 586
Size: 30 3704
BseYI C'CCAG_C
Cuts at: 1159 1917 2582 2990 1159
Size: 758 665 408 1903
Fragments arranged by size:
1903 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 368 368
Size: 3734
BsiEI CG_ry'CG
Cuts at: 104 1276 1322 2552 104
Size: 1172 46 1230 1286
Fragments arranged by size:
1286 1230 1172 46
BsiHKAI G_wGCw'C
Cuts at: 748 1526 1716 2576 748
Size: 778 190 860 1906
Fragments arranged by size:
1906 860 778 190
BsmI GAATG_Cn'
Cuts at: 627 627
Size: 3734
Bsp1286I G_dGCh'C
Cuts at: 525 748 1359 1452 1526 1716 1778 2576 525
Size: 223 611 93 74 190 62 798 1683
Fragments arranged by size:
1683 798 611 223 190 93 74 62
BspHI T'CATG_A
Cuts at: 179 2166 179
Size: 1987 1747
BspMI ACCTGCnnnn'nnnn_
Cuts at: 784 998 1300 1681 784
Size: 214 302 381 2837
Fragments arranged by size:
2837 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2026 2080 3564 3729 50
Size: 1976 54 1484 165 55
Fragments arranged by size:
1976 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1646 1646
Size: 3734
BsrFI r'CCGG_y
Cuts at: 1732 1913 1732
Size: 181 3553
BsrGI T'GTAC_A
Cuts at: 653 653
Size: 3734
BssSI C'ACGA_G
Cuts at: 2005 2713 2005
Size: 708 3026
BstAPI GCAn_nnn'nTGC
Cuts at: 622 1111 622
Size: 489 3245
BstBI TT'CG_AA
Cuts at: 2095 2095
Size: 3734
BstXI CCAn_nnnn'nTGG
Cuts at: 591 591
Size: 3734
BstYI r'GATC_y
Cuts at: 1 447 759 1226 1249 1584 1830 2136 2148
Size: 446 312 467 23 335 246 306 12
Cuts at: 2148 2234 3286 1
Size: 86 1052 449
Fragments arranged by size:
1052 467 449 446 335 312 306 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1684 1865 2028 1684
Size: 181 163 3390
Fragments arranged by size:
3390 181 163
BtsI GCAGTG_nn'
Cuts at: 977 977
Size: 3734
DraI TTT'AAA
Cuts at: 733 733
Size: 3734
DrdI GACnn_nn'nnGTC
Cuts at: 274 1440 2784 274
Size: 1166 1344 1224
Fragments arranged by size:
1344 1224 1166
EaeI y'GGCC_r
Cuts at: 509 1319 1493 1884 1911 3256 509
Size: 810 174 391 27 1345 987
Fragments arranged by size:
1345 987 810 391 174 27
EagI C'GGCC_G
Cuts at: 1319 1319
Size: 3734
EarI CTCTTCn'nnn_
Cuts at: 1757 1967 1757
Size: 210 3524
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1942 2684 2830 3544 1942
Size: 742 146 714 2132
Fragments arranged by size:
2132 742 714 146
EcoICRI GAG'CTC
Cuts at: 746 746
Size: 3734
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 179 432 1559 1991 2344 3006 154
Size: 25 253 1127 432 353 662 882
Fragments arranged by size:
1127 882 662 432 353 253 25
EcoO109I rG'GnC_Cy
Cuts at: 873 873
Size: 3734
EcoRI G'AATT_C
Cuts at: 738 738
Size: 3734
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1753 1753
Size: 3734
FspI TGC'GCA
Cuts at: 1515 1515
Size: 3734
HaeII r_GCGC'y
Cuts at: 1168 1416 2646 3417 1168
Size: 248 1230 771 1485
Fragments arranged by size:
1485 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1576 1606 1608 1638 1576
Size: 30 2 30 3672
Fragments arranged by size:
3672 30 30 2
HincII GTy'rAC
Cuts at: 773 773
Size: 3734
KasI G'GCGC_C
Cuts at: 1164 1412 1164
Size: 248 3486
KpnI G_GTAC'C
Cuts at: 754 754
Size: 3734
MluI A'CGCG_T
Cuts at: 3167 3167
Size: 3734
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2493 2677 2493
Size: 184 3550
MscI TGG'CCA
Cuts at: 511 1495 511
Size: 984 2750
MslI CAynn'nnrTG
Cuts at: 589 1723 1850 589
Size: 1134 127 2473
Fragments arranged by size:
2473 1134 127
MspA1I CmG'CkG
Cuts at: 845 1159 1519 2303 2548 845
Size: 314 360 784 245 2031
Fragments arranged by size:
2031 784 360 314 245
NaeI GCC'GGC
Cuts at: 1915 1915
Size: 3734
NarI GG'CG_CC
Cuts at: 1165 1413 1165
Size: 248 3486
NgoMIV G'CCGG_C
Cuts at: 1913 1913
Size: 3734
NspI r_CATG'y
Cuts at: 787 1287 2890 787
Size: 500 1603 1631
Fragments arranged by size:
1631 1603 500
PciI A'CATG_T
Cuts at: 2886 2886
Size: 3734
PmeI GTTT'AAAC
Cuts at: 733 733
Size: 3734
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 586 618 2180 2212 586
Size: 32 1562 32 2108
Fragments arranged by size:
2108 1562 32 32
PstI C_TGCA'G
Cuts at: 724 781 1466 724
Size: 57 685 2992
Fragments arranged by size:
2992 685 57
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3734
PvuII CAG'CTG
Cuts at: 1159 1519 1159
Size: 360 3374
RsrII CG'GwC_CG
Cuts at: 1929 1929
Size: 3734
SacI G_AGCT'C
Cuts at: 748 748
Size: 3734
SalI G'TCGA_C
Cuts at: 771 771
Size: 3734
SapI GCTCTTCn'nnn_
Cuts at: 1757 1967 1757
Size: 210 3524
SbfI CC_TGCA'GG
Cuts at: 781 781
Size: 3734
ScaI AGT'ACT
Cuts at: 3251 3251
Size: 3734
SfcI C'TryA_G
Cuts at: 33 339 537 720 777 1462 2430 2621 33
Size: 306 198 183 57 685 968 191 1146
Fragments arranged by size:
1146 968 685 306 198 191 183 57
SfoI GGC'GCC
Cuts at: 1166 1414 1166
Size: 248 3486
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3734
SmaI CCC'GGG
Cuts at: 756 756
Size: 3734
SmlI C'TyrA_G
Cuts at: 628 889 1804 2518 2780 628
Size: 261 915 714 262 1582
Fragments arranged by size:
1582 915 714 262 261
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3734
SphI G_CATG'C
Cuts at: 787 1287 787
Size: 500 3234
StuI AGG'CCT
Cuts at: 529 706 529
Size: 177 3557
StyI C'CwwG_G
Cuts at: 248 575 701 868 1222 248
Size: 327 126 167 354 2760
Fragments arranged by size:
2760 354 327 167 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1317 1450 1909 2118 1317
Size: 133 459 209 2933
Fragments arranged by size:
2933 459 209 133
TatI w'GTAC_w
Cuts at: 215 653 3249 215
Size: 438 2596 700
Fragments arranged by size:
2596 700 438
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3251 3283 3251
Size: 32 3702
Tth111I GACn'n_nGTC
Cuts at: 1531 1531
Size: 3734
XbaI T'CTAG_A
Cuts at: 765 765
Size: 3734
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 255 1229 255
Size: 974 2760
XmaI C'CCGG_G
Cuts at: 754 754
Size: 3734
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AlfI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AleI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BseRI BsiWI BsmBI BspEI BssHII BstEII BstZ17I Bsu36I
BtgI ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI