Restriction map for pF1KB5383
 (Linear) (Six-base) MAPSORT of: KIBB5383.seq  Check: 8229  from: 1  to: 1617



KIBB5383.orf

 With 182 enzymes: SgfI * 

                        September 18, 2009 17:00  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      87     355    1617 
   Size:         87     268    1262

  Fragments arranged by size: 

                1262     268      87
 
AfeI AGC'GCT

Cuts at:      0    1207    1617 
   Size:       1207     410
 
AflIII A'CryG_T

Cuts at:      0     449    1617 
   Size:        449    1168
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1508    1540    1617 
   Size:       1508      32      77

  Fragments arranged by size: 

                1508      77      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1533    1617 
   Size:       1533      84
 
ApoI r'AATT_y

Cuts at:      0     702     852    1000    1617 
   Size:        702     150     148     617

  Fragments arranged by size: 

                 702     617     150     148
 
AseI AT'TA_AT

Cuts at:      0    1478    1617 
   Size:       1478     139
 
AvaI C'yCGr_G

Cuts at:      0       4    1617 
   Size:          4    1613
 
BanI G'GyrC_C

Cuts at:      0      15      64     313    1617 
   Size:         15      49     249    1304

  Fragments arranged by size: 

                1304     249      49      15
 
BanII G_rGCy'C

Cuts at:      0    1260    1617 
   Size:       1260     357
 
BbeI G_GCGC'C

Cuts at:      0      68    1617 
   Size:         68    1549
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     998    1617 
   Size:        998     619
 
BbvCI CC'TCA_GC

Cuts at:      0    1085    1617 
   Size:       1085     532
 
BglII A'GATC_T

Cuts at:      0     609    1617 
   Size:        609    1008
 
BlpI GC'TnA_GC

Cuts at:      0    1202    1617 
   Size:       1202     415
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1265    1617 
   Size:       1265     352
 
Bpu10I CC'TnA_GC

Cuts at:      0    1085    1617 
   Size:       1085     532
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     275    1105    1617 
   Size:        275     830     512

  Fragments arranged by size: 

                 830     512     275
 
BsaHI Gr'CG_yC

Cuts at:      0      65    1617 
   Size:         65    1552
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1102    1617 
   Size:       1102     515
 
BseYI C'CCAG_C

Cuts at:      0     588    1065    1617 
   Size:        588     477     552

  Fragments arranged by size: 

                 588     552     477
 
BsiEI CG_ry'CG

Cuts at:      0      54    1617 
   Size:         54    1563
 
BsiHKAI G_wGCw'C

Cuts at:      0    1281    1554    1617 
   Size:       1281     273      63

  Fragments arranged by size: 

                1281     273      63
 
BsmI GAATG_Cn'

Cuts at:      0     272    1583    1617 
   Size:        272    1311      34

  Fragments arranged by size: 

                1311     272      34
 
Bsp1286I G_dGCh'C

Cuts at:      0    1260    1281    1554    1617 
   Size:       1260      21     273      63

  Fragments arranged by size: 

                1260     273      63      21
 
BspHI T'CATG_A

Cuts at:      0    1215    1617 
   Size:       1215     402
 
BsrBI CCG'CTC

Cuts at:      0      26    1617 
   Size:         26    1591
 
BsrDI GCAATG_nn'

Cuts at:      0     332    1617 
   Size:        332    1285
 
BsrFI r'CCGG_y

Cuts at:      0      46    1405    1445    1617 
   Size:         46    1359      40     172

  Fragments arranged by size: 

                1359     172      46      40
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     270    1509    1617 
   Size:        270    1239     108

  Fragments arranged by size: 

                1239     270     108
 
BstYI r'GATC_y

Cuts at:      0      95     609    1617 
   Size:         95     514    1008

  Fragments arranged by size: 

                1008     514      95
 
BtgI C'CryG_G

Cuts at:      0      54    1617 
   Size:         54    1563
 
BtsI GCAGTG_nn'

Cuts at:      0     575    1617 
   Size:        575    1042
 
DraI TTT'AAA

Cuts at:      0     851    1100    1617 
   Size:        851     249     517

  Fragments arranged by size: 

                 851     517     249
 
DraIII CAC_nnn'GTG

Cuts at:      0    1330    1617 
   Size:       1330     287
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     450    1617 
   Size:        450    1167
 
EaeI y'GGCC_r

Cuts at:      0      51    1617 
   Size:         51    1566
 
EagI C'GGCC_G

Cuts at:      0      51    1617 
   Size:         51    1566
 
EarI CTCTTCn'nnn_

Cuts at:      0     827    1246    1617 
   Size:        827     419     371

  Fragments arranged by size: 

                 827     419     371
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     105    1617 
   Size:        105    1512
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      87     355    1265    1617 
   Size:         87     268     910     352

  Fragments arranged by size: 

                 910     352     268      87
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     424    1617 
   Size:        424    1193
 
EcoO109I rG'GnC_Cy

Cuts at:      0     417    1617 
   Size:        417    1200
 
EcoRI G'AATT_C

Cuts at:      0    1000    1617 
   Size:       1000     617
 
HaeII r_GCGC'y

Cuts at:      0      68    1209    1617 
   Size:         68    1141     408

  Fragments arranged by size: 

                1141     408      68
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     872     904    1617 
   Size:        872      32     713

  Fragments arranged by size: 

                 872     713      32
 
HindIII A'AGCT_T

Cuts at:      0     364     637    1617 
   Size:        364     273     980

  Fragments arranged by size: 

                 980     364     273
 
KasI G'GCGC_C

Cuts at:      0      64    1617 
   Size:         64    1553
 
MfeI C'AATT_G

Cuts at:      0     650    1016    1160    1617 
   Size:        650     366     144     457

  Fragments arranged by size: 

                 650     457     366     144
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     412    1526    1617 
   Size:        412    1114      91

  Fragments arranged by size: 

                1114     412      91
 
MspA1I CmG'CkG

Cuts at:      0      56    1617 
   Size:         56    1561
 
NaeI GCC'GGC

Cuts at:      0      48    1407    1617 
   Size:         48    1359     210

  Fragments arranged by size: 

                1359     210      48
 
NarI GG'CG_CC

Cuts at:      0      65    1617 
   Size:         65    1552
 
NgoMIV G'CCGG_C

Cuts at:      0      46    1405    1617 
   Size:         46    1359     212

  Fragments arranged by size: 

                1359     212      46
 
NotI GC'GGCC_GC

Cuts at:      0      51    1617 
   Size:         51    1566
 
NspI r_CATG'y

Cuts at:      0     453    1065    1453    1617 
   Size:        453     612     388     164

  Fragments arranged by size: 

                 612     453     388     164
 
PasI CC'CwG_GG

Cuts at:      0    1455    1617 
   Size:       1455     162
 
PciI A'CATG_T

Cuts at:      0     449    1617 
   Size:        449    1168
 
PstI C_TGCA'G

Cuts at:      0    1473    1617 
   Size:       1473     144
 
SacII CC_GC'GG

Cuts at:      0      57    1617 
   Size:         57    1560
 
SfcI C'TryA_G

Cuts at:      0    1424    1469    1617 
   Size:       1424      45     148

  Fragments arranged by size: 

                1424     148      45
 
SfoI GGC'GCC

Cuts at:      0      66    1617 
   Size:         66    1551
 
SmlI C'TyrA_G

Cuts at:      0     290    1120    1617 
   Size:        290     830     497

  Fragments arranged by size: 

                 830     497     290
 
SphI G_CATG'C

Cuts at:      0    1453    1617 
   Size:       1453     164
 
SspI AAT'ATT

Cuts at:      0    1315    1617 
   Size:       1315     302
 
StyI C'CwwG_G

Cuts at:      0     153    1617 
   Size:        153    1464
 
SwaI ATTT'AAAT

Cuts at:      0    1100    1617 
   Size:       1100     517
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     703     833    1617 
   Size:        703     130     784

  Fragments arranged by size: 

                 784     703     130
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     270     774    1617 
   Size:        270     504     843

  Fragments arranged by size: 

                 843     504     270
 
XmnI GAAnn'nnTTC

Cuts at:      0    1574    1617 
   Size:       1574      43
 

 Enzymes that do cut:

     AcuI     AfeI   AflIII     AloI    AlwNI     ApoI     AseI     AvaI
     BanI    BanII     BbeI     BbsI    BbvCI    BglII     BlpI     BpmI
   Bpu10I    BpuEI    BsaHI    BseRI    BseYI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspHI    BsrBI    BsrDI    BsrFI    BstXI    BstYI     BtgI
     BtsI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  Eco57MI    EcoNI EcoO109I    EcoRI    HaeII    Hin4I  HindIII     KasI
     MfeI     MmeI   MspA1I     NaeI     NarI   NgoMIV     NotI     NspI
     PasI     PciI     PstI    SacII     SfcI     SfoI     SmlI     SphI
     SspI     StyI     SwaI    TaqII     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII     AgeI     AhdI
     AleI     AlfI     ApaI    ApaLI     AscI    AsiSI    AvrII     BaeI
    BamHI     BcgI    BciVI     BclI    BfrBI     BglI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI
     BsgI    BsiWI    BsmBI    BspEI    BspMI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI
  EcoICRI    EcoRV     FalI     FseI     FspI    FspAI   HincII     HpaI
     KpnI     MluI     MscI     MslI     NcoI     NdeI     NheI     NruI
     NsiI     PacI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     StuI     TatI
     TstI  Tth111I     XbaI     XhoI     XmaI     ZraI
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