Restriction map for pF1KB5346
##################################
# NO CUT ENZYMES (  104 enzymes) #
##################################

Acc65I      AccI        AflII       AgeI        AocI        
Aor51HI     ApaI        AscI        AseI        AsnI        
Asp700I     Asp718I     AvrII       BamHI       BanI        
BbeI        BbrPI       BbvCI       BciVI       BclI        
BfaI        BfrI        BglI        BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsiEI       BsiWI       
BspDI       BspHI       BsrBI       BsrDI       BsrFI       
BssHII      Bst1107I    BstBI       BstEII      BstPI       
BstXI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DraIII      DrdI        EagI        
EclXI       Eco47III    Eco52I      Eco81I      EcoO65I     
EcoRI       EcoRV       FbaI        FseI        HincII      
HindII      HpaI        KasI        KpnI        MaeI        
MamI        MfeI        MluI        MunI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
PvuI        PvuII       RcaI        RsrII       SalI        
SapI        SbfI        ScaI        SfiI        SfoI        
SfuI        SgrAI       SnaBI       SpeI        Sse8387I    
StuI        SwaI        XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AatII        1424  GACGTC               GACGT^C
AccIII        215  TCCGGA               T^CCGGA
AclI         1452  AACGTT               AA^CGTT
AcsI         1435  AAATTT               [GA]^AATT[CT]
AflIII         11  ACATGT               A^C[GA][CT]GT
AhdI            7  GACAACATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1229  GTGCAC               G^TGCAC
ApaLI        1229  GTGCAC               G^TGCAC
ApoI         1435  AAATTT               [GA]^AATT[CT]
AspEI           7  GACAACATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1582  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         321  TGCGCA               TGC^GCA
BcgI          324  GCAGGTTCGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII        1100  AGATCT               A^GATCT
BsaI         1475  GGTCTC               GGTCTC(1/5)
BsaWI         215  TCCGGA               [AT]^CCGG[AT]
BseAI         215  TCCGGA               T^CCGGA
BsgI          590  GTGCAG               GTGCAG(16/14)
BsmBI        1784  CGTCTC               CGTCTC(1/5)
BspEI         215  TCCGGA               T^CCGGA
BspLU11I       11  ACATGT               A^CATGT
BspMI         324  GCAGGT               ACCTGC(4/8)
DraI         1665  TTTAAA               TTT^AAA
DsaI          596  CCGCGG               C^C[GA][CT]GG
Eam1105I        7  GACAACATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          396  GAAGAG               CTCTTC(1/4)
EcoT22I      1282  ATGCAT               ATGCA^T
FauI          628  CCCGC                CCCGC(4/6)
FspI          321  TGCGCA               TGC^GCA
HaeII         680  GGCGCT               [GA]GCGC^[CT]
Ksp632I       396  GAAGAG               CTCTTC(1/4)
KspI          596  CCGCGG               CCGC^GG
MroI          215  TCCGGA               T^CCGGA
MspA1I        596  CCGCGG               C[AC]G^C[GT]G
NsiI         1282  ATGCAT               ATGCA^T
PaeR7I       1096  CTCGAG               C^TCGAG
PflFI        1582  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         648  CCACAACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          298  GAGTC                GAGTC(4/5)
Psp1406I     1452  AACGTT               AA^CGTT
SacII         596  CCGCGG               CCGC^GG
SexAI         653  ACCTGGT              A^CC[AT]GGT
SmaI         1777  CCCGGG               CCC^GGG
SmlI         1096  CTCGAG               C^T[CT][GA]AG
SphI         1284  GCATGC               GCATG^C
SspI         1076  AATATT               AAT^ATT
TaqI         1097  TCGA                 T^CGA
Tsp45I        222  GTCAC                ^GT[GC]AC
Tth111I      1582  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I      1097  TCGA                 T^CGA
Van91I        648  CCACAACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          515  CCAAAGAGCCTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         1096  CTCGAG               C^TCGAG
XmaCI        1777  CCCGGG               C^CCGGG
XmaI         1777  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AvaI         1096  CTCGAG               C^[CT]CG[GA]G
             1777  CCCGGG              
AvaII         723  GGACC                G^G[AT]CC
             1120  GGACC               
BalI          371  TGGCCA               TGG^CCA
              761  TGGCCA              
BsmFI        1119  GGGAC                GGGAC(10/14)
             1327  GGGAC               
BsmI          288  GAATGC               GAATGC(1/-1)
             1303  GAATGC              
BsoBI        1096  CTCGAG               C^[CT]CG[GA]G
             1777  CCCGGG              
BssSI        1754  CACGAG               CACGAG(-5/-1)
             1226  CTCGTG              
BstAPI        309  GCAGAAACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              384  GCAGAAACTGC         
EcoNI         246  CCTGAGTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1606  CCTGACGGAGG         
HindIII        52  AAGCTT               A^AGCTT
              107  AAGCTT              
MaeIII        222  GTCAC                ^GT[ACGT]AC
             1387  GTTAC               
MluNI         371  TGGCCA               TGG^CCA
              761  TGGCCA              
MscI          371  TGGCCA               TGG^CCA
              761  TGGCCA              
MseI         1024  TTAA                 T^TAA
             1666  TTAA                
NspI           11  ACATGT               [GA]CATG^[CT]
             1284  GCATGC              
PpuMI         722  AGGACCT              [GA]G^G[AT]CC[CT]
             1119  GGGACCT             
PstI          391  CTGCAG               CTGCA^G
             1445  CTGCAG              
SacI          238  GAGCTC               GAGCT^C
              965  GAGCTC              
Tru9I        1024  TTAA                 T^TAA
             1666  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   80 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       AspHI       BanII       BbsI        
BbvI        BcnI        BmyI        BpmI        BpuAI       
BsaHI       BsaJI       BseRI       BsiHKAI     BsiYI       
BslI        BsmAI       Bsp1286I    Bsp1407I    BsrGI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       EaeI        EcoO109I    EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        MaeII       MboI        
MboII       MflI        MnlI        MslI        MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SspBI       StyI        
TfiI        TseI        Tsp509I     TspRI       XhoII       
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